Antimicrobial Activity of Nanostructured Lipid Carriers Loaded Punica granatum Seed Oil against Staphylococcus epidermidis

2020 ◽  
Vol 8 (6) ◽  
pp. 485-494
Author(s):  
Nazanin Fathi ◽  
Farzaneh Lotfipour ◽  
Solmaz M. Dizaj ◽  
Hamed Hamishehkar ◽  
Maryam Mohammadi
Keyword(s):  
Antimicrobial Activity ◽  
Particle Size ◽  
Size Distribution ◽  
Staphylococcus Epidermidis ◽  
Seed Oil ◽  
Antimicrobial Agents ◽  
Antioxidant Properties ◽  
Punica Granatum ◽  
Nanostructured Lipid Carriers ◽  
Oil Emulsion

Background: Nanostructured lipid carriers (NLCs), due to their impressive benefits, have recently been considered in different areas. Besides, NLC loaded with essential oils is attractive for finding more effective antimicrobial products, especially against common bacteria such as Staphylococcus epidermidis (S. epidermidis). Objective: This study aims to prepare and characterize NLCs encapsulated with Punica granatum (P. granatum) seed oil (PGS oil-loaded NLCs) and examine the antimicrobial effect of this combination against S. epidermidis. Methods: PGS oil-loaded NLCs were prepared using a hot melt homogenization method. Later, they were characterized by determining particle size distribution (particle size analyzer), morphology (scanning electron microscopy (SEM)), and zeta potential (surface charge of NLCs). Minimum inhibitory concentrations (MIC) of PGS oil-loaded NLCs were assessed and compared with seed oil emulsion of P. granatum against S. epidermidis. Results: PGS oil-loaded NLCs were spherical shaped nanoparticles, with a mean size of 102.10 nm and narrow size distribution (PDI = 0.26). The antibacterial assay showed PGS oil-loaded NLCs to have a higher in vitro antimicrobial activity than seed oil emulsion of P. granatum. Conclusion: To conclude, NLCs may be a favorable carrier to develop new antimicrobial agents. Lay Summary: The lipid nanoparticles such as nanostructured lipid carriers (NLCs) appeared as products first on the cosmetic market. Their advantages help them to be used in different healthcare and cosmetic products. With regard to previous studies, Punica granatum (P. granatum) extract shows antimicrobial and antioxidant properties that could be a valuable natural source against the wide ranges of bacteria. Then, P. granatum seed oil (PGS oil-loaded NLCs) prepared in this study can be used in dental and skin-related materials as a new natural antimicrobial product.

Role of ZnO Nanoparticles for improvement of Antibacterial Activity in Food Packaging

2021 ◽  
pp. 128-131
Author(s):  
Saira Sehar ◽  
Amiza Amiza ◽  
I. H Khan
Keyword(s):  
Antimicrobial Activity ◽  
Particle Size ◽  
Surface Area ◽  
Zno Nanoparticles ◽  
Food Packaging ◽  
Antimicrobial Agents ◽  
Uv Light ◽  
Zno Nps ◽  
Long Time

Nanotechnology advancement leads to development of antimicrobial agents like ZnO nanoparticles. These nanoparticle have their main applications in food packaging. when these nanoparticles incorporate into the food surface, it will kill all bacterias residing on the surface and food become free of bacteria. In this way, food can be stored for a long time because its shelf life is improved. Antimicrobial activity of ZnO nanoparticles can be improved by increasing surface area, reducing particle size and large concentration of ZnO –NPS. Antimicrobial activity increases by increasing intensity of UV light. As UV light fall on ZnO nanoparticles, it increases ZnO surface area and hence anrtimicrobial activity will be increased. Exact mechanism of Antimicrobial activity is still unknown but some processes have been presented.

scholarly journals Micronized Oat Husk: Particle Size Distribution, Phenolic Acid Profile and Antioxidant Properties

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5443
Author(s):  
Dariusz Dziki ◽  
Wojciech Tarasiuk ◽  
Urszula Gawlik-Dziki
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Phenolic Acids ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffraction Method ◽  
Total Phenolic ◽  
Oat Husk

Oat husk (OH; hull) is a by-product generated from oat processing and is rich in insoluble fibre and phenolic compounds. The aim of this work was to study the particle size distribution, antioxidant activity, and phenolic profile of micronized OH. For this purpose, the hull was first sterilized using superheated steam and was then ground using an impact classifier mill. The particle size distribution (PSD) of the ground husk was determined using the laser diffraction method and the parameters characterizing the PSD of the ground husk, and its antioxidant activity were calculated. In addition, UPLC-MS/MS analysis of phenolic acids was also performed. Micronization of the sterilized husk effectively decreased the size of the particles, and with the increasing speed of the rotor and classifier, the median size of the particles (d50) decreased from 63.8 to 16.7 µm. The following phenolic acids were identified in OH: ferulic, caffeic, p-hydroxybenzoic, vanillic, syringic, and synapic acid. Ferulic acid constituted about 95% of total phenolic acids. The antioxidant activity of the obtained extracts increased as the particle size of the micronized husk decreased. The highest half maximal inhibitory concentration (EC50 index) was found for chelating power, and the lowest was found in the case of radical scavenging activity against DPPH.

Chemical screening and biological activities of extracts from Cistus munbyi Pomel

2020 ◽  
Vol 06 ◽  
Author(s):  
Fethi Benbelaïd ◽  
Abdelmounaїm Khadir ◽  
Yacine Benziane ◽  
Asma Bennaissa ◽  
Mourad Bendahou
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Antimicrobial Agents ◽  
Biological Activities ◽  
Local Population ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Scavenging Activity ◽  
Chemical Screening

Background: Cistus munbyi Pomel is a well-known medicinal plant in Western Algeria for its several applications in medicine and food. This endemic species to North Africa is poorly studied among the genus of Cistus, which gives the opportunity to carry out original pharmacological studies to value this medicinal plant. Objective: To investigate the antioxidant and the antimicrobial activities of Cistus munbyi, an endemic medicinal plant to Algeria. Methods: The antioxidant properties of C. munbyi crudes extracts were evaluated using the DPPH radical-scavenging activity tests. While their antimicrobial activity was studied by the agar diffusion method and MICs determination against eighteen bacterial and fungal strains, some of which were clinical. Results: The obtained results showed that aqueous and ethanol extracts possess a strong scavenging activity against DPPH radicals with EC50 values estimated at 1.12 and 8.31 µg/mL, respectively. On the other hand, extracts from C. munbyi revealed homogeneous and good antimicrobial activity, in which the inhibition zone diameters observed were ranging from 08 to 19 mm, and the MICs values were included between 0.312 and 5.000 mg/mL. Aqueous extract was the less active except against Proteus mirabilis and Staphylococcus aureus. Conclusion: The findings of this study indicate that C. munbyi possess a strong antioxidant activity and an interesting antimicrobial potential, which allow justifying its uses by the local population in several applications. Therefore, C. munbyi could be as a good source of natural antioxidants and antimicrobial agents.

Rape Seed Oil B100 Diesel Engine Particulate Emissions: The Influence of Intake Oxygen on Particle Size Distribution

2012 ◽  
Author(s):  
Seyed Ali. Hadavi ◽  
Hu Li ◽  
Patrick Biller ◽  
Amanda Lea-Langton ◽  
Gordon Andrews ◽  
...  
Keyword(s):  
Particle Size ◽  
Diesel Engine ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Seed Oil ◽  
Particulate Emissions ◽  
Rape Seed

Factors Evalution of Particle Size Distribution of Heavy Oil Emulsion

2013 ◽  
Vol 800 ◽  
pp. 389-392
Author(s):  
Nan Ning Wei
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Heavy Oil ◽  
Oil Field ◽  
Impact Factors ◽  
Average Particle ◽  
Agitation Rate ◽  
Oil Emulsion ◽  
The Impact

Used the heavy oil of Du 84 well 53-55 in Liaohe oil field, the impact factors of the heavy oil emulsion size distribution were analyzed. The higher concentration of emulsifier, the smaller size of average particle emulsion and the particle size distribution tends to be more uniform. With the stirring rate increasing the size distribution tends to be uniform first then tend to be disperse, and the time used would be shorter to reach equilibrium with agitation rate being faster.With prolong of the stirring time the particle size distribution of the emulsion trends to be more uniform. After emulsifier 0.3%LZC-C-02 being added in heavy oil it will become O/W emulsion and the external phase is water and the viscosity of the emulsion is small with good flowability.

scholarly journals PHYSICAL, CHEMICAL CHARACTERISTICS AND RELEASE RATE OF MELOXICAM FROM NANOSTRUCTURED LIPID CARRIERS (NLC) SYSTEM USING SOME CONCENTRATION RATIOS OF MONOSTEARIN AND ALPHA TOCOPHEROL ACETATE LIPID MATRIX

2017 ◽  
Vol 10 (2) ◽  
pp. 132
Author(s):  
Yulia Anggraeni ◽  
Isnaeni Isnaeni ◽  
Esti Hendradi
Keyword(s):  
Particle Size ◽  
Size Distribution ◽  
Entrapment Efficiency ◽  
Nanostructured Lipid Carriers ◽  
Alpha Tocopherol ◽  
Chemical Characteristics ◽  
Lipid Matrix ◽  
Physical And Chemical Characteristics ◽  
Tocopherol Acetate ◽  
Physical And Chemical

Objective: The objective of this research is to develop nanostructured lipid carriers (NLC) of meloxicam (MLX) for topical application using monostearin and alpha-tocopherol as lipid matrix.Methods: MLX-NLC was prepared by high-shear homogenization, and it was characterized for organoleptic, pH, viscosity, particle size and size distribution, zeta potential, crystallinity, morphology, and entrapment efficiency. The six different ratios of solid and liquid lipid matrix were70:30, 75:25, 80:20, 85:15, 90:10, and 95:5, respectively.Results: The pH value of all formulas met the pH range of topical dosage at 4.5-6.5. Viscosity test showed that a trend toward the decrease in viscosity with the increase in alpha-tocopherol content was observed. The results of particle size test proved that all six NLC system formulas have <1000 nm particle size with a quite narrow particle size distribution. Scanning electron microscope revealed nearly spherical shape NLC with negligible effect ratios of solid and liquid lipid on the particle morphology. The X-ray diffraction result showed the decreasing of peaks intensity of MLX-NLC system. It showed that MLX in NLC system is amorphous. The result showed that the increasing concentration of liquid lipid in NLC system caused the rising entrapment efficiency of MLX.Conclusion: The result of this research indicates that the concentration ratio of monostearin and alpha-tocopherol acetate affects the physical and chemical characteristics of NLC system.Keywords: Nanostructured lipid carriers, Meloxicam, Monostearin, Physical and chemical characteristics, Entrapment efficiency. 

scholarly journals A Novel Functional Emulsifier Prepared with Modified Cassava Amylose with Octenyl Succinic Anhydride and Quercetin: Preparation and Application in the Pickering Emulsion

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6884
Author(s):  
Hailing Zhang ◽  
Haiming Chen ◽  
Shan Jiang ◽  
Xiaoning Kang
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Succinic Anhydride ◽  
Structural Characteristics ◽  
Antioxidant Properties ◽  
Oxidation Stability ◽  
Infrared Spectrometry ◽  
Octenyl Succinic Anhydride ◽  
Carbohydrate Polymer

An emulsifier with a targeted antioxidant effect was prepared using the inclusion complexes of octenyl succinic anhydride (OSA)-modified cassava amylose (CA) and quercetin (Q). The designed emulsifier, a carbohydrate polymer-flavonoid complex, exhibited both amphiphilic and antioxidant properties. To investigate the physical and oxidation stabilities of the prepared emulsion, three types of emulsions were prepared: primary emulsions stabilized by enzyme-modified starch, secondary emulsions stabilized by OSA-CA, and tertiary emulsions stabilized by Q-encapsulated complexes (OSA-CA/Q). The structural characteristics of CA, OSA-CA, and OSA-CA/Q were investigated by scanning electron microscopy, Fourier transform infrared spectrometry, and small-angle X-ray scattering analysis. The stabilities of the emulsions were evaluated based on their particle size distribution, zeta potential, creaming stability, and peroxide value. The results showed that the secondary and tertiary emulsions exhibited a relatively narrower particle size distribution than the primary emulsions, but the particle size distribution of the tertiary emulsions was the narrowest (10.42 μm). Moreover, the secondary and tertiary emulsions had lower delamination indices than the primary emulsions after 7 days of storage. The results obtained from the antioxidant experiments indicated that OSA-CA/Q exhibited good oxidation stability for application in emulsion systems.

Sign in / Sign up

Export Citation Format

Share Document