Pengaruh pengencer air kelapa tua yang berbeda varietas terhadap kualitas semen cair Kambing Boer pada penyimpanan 3-50C

Artificial Insemination using liquid semen is technology reproduction can improve goat productivity without liquid nitrogen. The Purpose of this research was to determine differences of old coconut water varieties of red (Cocos rubescens) and green (Cocos viridis) as diluent Boer goat semen, based on motility, viability and spermabnormalities during storage at 3-50C. The materials used for this research was Boer goat fresh semen as much as 3 heads were collected 2 times a week. Experimental Design. The treatment were devided into three Treatmen, there were: P0 (Tris+ 20% egg yolk); P1 (old coconut water varieties red + 20% egg yolk); P2 (old coconut water varieties green + 20% egg yolk). The data were analyzed by randomized block design, if the result are different or significantly different then continued to be tested with Duncan's multiple range test. The result showed that during storage until days 4, P0 has the best result on motility (40,5 + 1,1 %), viability (45,18+ 1,56 %), and abnormality (1,89+ 0,59 %). While on the diluent using old coconut water green variety better then red variety. On day of 2 P1 and P2 have motility (61 + 1,3%) and (51+ 7,5%); Viability (67,14+17,99%) and (55,37+10,66%), abnormality (1,23 + 1,02%) dan (1,70+ 0,99%).

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Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.5449 ◽

2019 ◽

Vol 6 (1) ◽

pp. 78

Author(s):

Muhammad Ade Salim ◽

Muhammad Nur Ihsan ◽

Nur Isnaini ◽

Trinil Susilawati

Keyword(s):

Semen Quality ◽

Block Design ◽

Animal Husbandry ◽

Egg Yolk ◽

Coconut Water ◽

Boer Goat ◽

Randomized Block Design ◽

Frozen Semen ◽

Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari 3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1: young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%), while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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Viability of Peranakan Etawah Liquid Semen Preserved in Tris Substituted with Various Energy Sources

Jurnal Ilmu Ternak dan Veteriner ◽

10.14334/jitv.v25i2.2026 ◽

2020 ◽

Vol 25 (2) ◽

pp. 68

Author(s):

Nurul Afzan Hilda Zakiya ◽

A H Yanti ◽

T R Setyawati

Keyword(s):

Energy Source ◽

Artificial Insemination ◽

Block Design ◽

Egg Yolk ◽

Energy Sources ◽

Randomized Block Design ◽

Frozen Semen ◽

Semen Preservation ◽

Semen Extender ◽

Fresh Semen

The use of liquid semen for artificial insemination program of Etawah crossbreed goat (PE) is an alternative to replace frozen semen which is constrained by limited and expensive facilities. Production of liquid semen is faster than frozen semen, but the viability of liquid semen which preserved with a standard extender such as tris egg yolk is very short. The purpose of this study was to determine the viability of PE goat semen in egg yolk tris substituted with energy sources such as glucose, galactose, and mannose and to determine the most efficient energy source for semen preservation. This research was conducted from August to September 2018 at the Artificial Insemination Center in Lembang, West Java. This study was designed in a randomized block design (RBD) consist of three experimental groups divided into five groups. Fresh semen of PE goats were preserved using extender which energy source has been modified. Results showed that using glucose in PE goat semen extender produced the best motility among other groups (64.29 ± 9.2%). The highest viability was found in extender with fructose substitution (86.76 ± 2.3%). The longest viability of liquid semen was found in the extender with glucose substitution. It lasted for six days.

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PEMBERIAN BEBERAPA ZPT ORGANIK TERHADAP BEBERAPA VARIETAS BIBIT KELAPA SAWIT (Elaeis guineensis Jacq.) DI PRE-NURSERY

Jurnal Agrotek Lestari ◽

10.35308/jal.v5i1.1966 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Yazid Habiby Lubis ◽

Mita Setyowati ◽

Aboe B. Saidi

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Block Design ◽

Coconut Water ◽

Factor V ◽

Organic Growth ◽

Randomized Block Design ◽

Elaeis Guineensis Jacq ◽

Materials Used ◽

Experimental Garden

The aims of this study was to determine the effect of several organic growth regulatory on several varieties of oil palm seedlings (Elaeis guineensis Jacq) in Pre Nursery. The research was carried out in the experimental garden of the Faculty of Agriculture, Teuku Umar University, West Aceh, from March until finished. The materials used were Organic growth regulatory in the form of 50 cc coconut water, 50 cc union extract and 50 cc bamboo shoot extract. While the varieties of oil palm seedlings are varieties that yangambi, simalungun and 239 from IOPRI Medan. This study used a factorial randomized block design (RBD) 4 X 3 with 3 replications. Giving growth regulatory (Z) consists of 4 levels, namely: Z0 = Control, Z1 = Coconut water, Z2 = Shallot Extract, Z3 = Bamboo Extract, while Variety factor (V) consists of 3 levels: V1 = Yangambi, V2 = Simalungun, V3 = 239. Observation parameters are increase in seedling height (cm), stem diameter (mm), number of leaf midribs (midrib), wet stover weight (g), number of roots (number) and root length (cm). Keywords: Growth Regulatory, Varieties, Oil Palm, Pre Nursery

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Kidding rate of artificial insemination with Boer goat liquid semen during chilled preservation using coconut water-based diluent

Jurnal Ilmu-Ilmu Peternakan ◽

10.21776/ub.jiip.2020.030.03.02 ◽

2020 ◽

Vol 30 (3) ◽

pp. 184-189

Author(s):

Muhammad Ade Salim ◽

Muhammad Nur Ihsan ◽

Nurul Isnaini ◽

Trinil Susilawati

Keyword(s):

Egg Yolk ◽

Return Rate ◽

Coconut Water ◽

Chi Square ◽

Boer Goat ◽

Estrous Synchronization ◽

Water Based ◽

Materials Used ◽

Chi Square Analysis

The quality of liquid semen with coconut water as diluent could be preserved up to 3 to 4 days during cold storage at 4 - 5oC. In this research, the successful insemination rate of coconut water-based diluent Boer goat liquid semen was observed to understand the application of coconut water as semen diluent. The materials used in this research were 5 to 7 months Viridis coconut water, liquid semen from 3 years old Boer buck, 30 does for acceptor, and Tris-aminomethane added with 10% egg yolk (EY) as a control diluent. The Boer semen was collected once a week by using artificial vagina, diluted and then stored before used for insemination. The insemination was done by using intra-cervical method at position 3, started with estrous synchronization by using 2 mL PGF2α. The research was conducted as experimental method under randomized group design with two treatments, that were Tris-aminomethane + 10% EY (P0) and coconut water + 10% EY (P1). All of the treatments were repeated for 15 times and the collected data were analyzed with Pearson’s chi square with Genstat 18 program. The observed variables include non-return rate and kidding rate. The results showed that the first non-return rate of P1 were 93.33% and in P0 were 73.33%, while the second non-return rate of P1 were 93.33% and in P0 were 80%. Furthermore, the kidding rate of P1 were 40% and P0 were 66.66%. The Pearson’s chi square analysis showed that there was no effect of coconut water as semen diluent to the kidding rate. The research concludes that the usage of coconut water as semen diluent did not affect the successful insemination rate of Boer goat.

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KUALITAS SEMEN CAIR SAPI SIMENTAL MENGGUNAKAN LARUTAN ISOTONIS KOMERSIAL PADA KONSENTRASI DAN LAMA PENYIMPANAN BERBEDA

JURNAL PETERNAKAN ◽

10.24014/jupet.v14i2.3676 ◽

2017 ◽

Vol 14 (2) ◽

pp. 70

Author(s):

Bayu Anke Mardian ◽

Zumarni Zumarni ◽

Anwar Effendi Harahap

Keyword(s):

Artificial Insemination ◽

Block Design ◽

Egg Yolk ◽

Isotonic Solution ◽

Factor B ◽

Randomized Block Design ◽

Duration Of Storage ◽

Alternative Materials ◽

Selection Of

Artificial insemination (AI) is a technique to insert the sperm or semen of bull thawed and processed first into the genital tract of female methods and a special tool called insemination gun, efforts to optimize the management of the cement in order to obtain the quality of cement is optimal to do with the selection of diluent cement. One alternative materials cement diluent is an isotonic solution Commercial (LIK). This study aimed to examine the interaction between the concentration and duration of storage of diluent solution isotonic different to the quality of spermatozoa cow simental. This research was conducted at the Regional Technical Implementation Unit of Artificial Insemination Centres (UPTD BIB) Tuah Sakato, Payakumbuh. In April 2016. This study used a randomized block design (RAK) factorial design to Factor A (Concentration LIK) ie A1 = 100 ml Tris Egg Yolk, A2 = (60 ml) Tris Egg Yolk + (40 ml) LIK, A3 = (55 ml) Tris Egg Yolk + (45 ml) LIK, A4 = (50 ml) Tris Egg Yolk + (50 ml) LIK, and factor B (retention) is B1 = 0 day, B2 = 1, B3 = 2 day, days, B4 = 3 days. Each treatment consists of three replicates. Parameters measured were motility, percentage of survival, abrnormalitas, and MPU. These results indicate that an isotonic solution can be used as an alternative Commercial diluent with the best results on the percentage LIK 40 ml, but the effectiveness of the efficiency of storage use was only on the first day (24 hours).

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Growth Response of Mucuna Cuttings to Concentration and Duration of Soaking Shallot Extract

AGRINULA: Jurnal Agroteknologi dan Perkebunan ◽

10.36490/agri.v4i2.162 ◽

2021 ◽

Vol 4 (2) ◽

pp. 122-129

Author(s):

Enda Sabda Gentri Sembiring ◽

Julaili Irni ◽

Rama Riana Sitinjak ◽

Bayu Pratomo

Keyword(s):

Growth Response ◽

Block Design ◽

Duncan Multiple Range Test ◽

Soaking Time ◽

Extract Concentration ◽

Survival Percentage ◽

Randomized Block Design ◽

Two Factors ◽

Number Of Leaves ◽

Range Test

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors

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PENGARUH KONSENTRASI PUPUK ORGANIK CAIR SERTA VARIETAS DAN GALUR TERHADAP PERTUMBUHAN DAN PRODUKSI BENIH TANAMAN KACANG PANJANG (Vigna sinensis (L.) Savi ex Hassk.)

ZIRAA'AH MAJALAH ILMIAH PERTANIAN ◽

10.31602/zmip.v46i3.4578 ◽

2021 ◽

Vol 46 (3) ◽

pp. 278

Author(s):

Arnandha Desya Refaldi ◽

Prasodjo Soedomo ◽

Muharam Muharam ◽

Yayu Sri Rahayu

Keyword(s):

Seed Weight ◽

Block Design ◽

Organic Fertilizer ◽

Agricultural Technology ◽

Vegetable Crops ◽

Duncan Multiple Range Test ◽

Randomized Block Design ◽

Main Plot ◽

Range Test ◽

Plot Design

Long bean production in Indonesia continues to decline every year, this is due to land conversion and the lack of application of agricultural technology. One of the efforts to increase long bean production is by multiplying quality seeds using organic materials as nutrients needed by plants. This study aims to determine the response of the growth and production of long bean seeds to the interaction of strains, varieties with a concentration of liquid organic fertilizer. The research was conducted at the Vegetable Crops Research Institute, Lembang, West Bandung Regency from July 2020 to January 2021. The research method used was an experimental method with a Randomized Block Design (RBD) with a split-plot design consisting of 2 factors: the first factor (main plot) (A) was the variety and strain with 2 levels, a1 (Tavi canton variety) and a2 (pras-1 strain).The second factor (subplot) (B) is concentration of biopras-1 with 5 levels, b1 (0 cc/l-1 water), b2 (1 cc/l-1 water), b3 (2 cc/l-1 water), b4 (3 cc/l-1 water) and b5 (4 cc/l-1 water). The effect of the treatment was analyzed by means of variance and if the f test at 5% level was significantly different, then to find out the best treatment was continued with the DMRT (Duncan Multiple Range Test) at the 5% level. The results showed that there was an interaction between varieties, lines with various concentrations of biopras-1 liquid organic fertilizer on dry seed weight per plant, dry seed weight per plot, and 1000 seeds weight. Treatment of pras-1 lines with the concentration of liquid organic fertilizer biopras-1 4 cc / l of water was able to give the best results on dry seed weight per plant (35.31 gr), dry seed weight per plot (1.298.20 gr or 2.16 Ton/ha-1) and the weight of 1000 seeds (153.10 gr).

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Kecernaan Nutrien pada Babi Lokal Periode Pertumbuhan yang Diberi Ransum Mengandung Biji Asam Biokonversi Spontan (NUTRIENTS DIGESTIBILITY IN GROWING LOCAL PIG FED WITH DIET COMPOSED OF SPONTENOUS BIOCONVERTED-TAMARIND SEED)

jurnal veteriner ◽

10.19087/jveteriner.2017.18.4.610 ◽

2018 ◽

Vol 18 (4) ◽

pp. 610

Author(s):

Redempta Wea ◽

I Gusti Komang Oka Wirawan ◽

Bernadete Barek Koten

Keyword(s):

Body Weight ◽

Crude Protein ◽

Dry Matter ◽

Animal Feed ◽

Block Design ◽

Nutrient Digestibility ◽

Tamarind Seed ◽

Randomized Block Design ◽

Range Test ◽

Nutrients Digestibility

A research aimed to evaluate nutrients digestibility in growing local male pig fed spontaneous bioconverted-tamarind seed has been done in animal feed technology laboratory of Kupang State Agricultural Polytechnic.The research used 25 local male grower pigs with body weight around 3-6 kg. The study used a randomized block design with five treatments, i.e. R0 = ration using tamarind seeds without bioconversion, R1 = ration using spontaneous bioconversion tamarinds for 24 hours, R2 = rations using spontaneous bioconversion tamarinds for 48 hours, R3 = rations using bioconversion tamarinds spontaneous for 72 hours, and R4 = rations using spontaneous bioconversion tamarinds for 96 hours. Each treatment was repeated five times in which each of them used five pigs. Parameters observed were nutrient digestibility (dry matter, crude protein, crude fat, crude fiber, and ash). The data were analyzed by using variance and continued by Duncan’s multiple range test to determine the differences between treatments. The results showed that the time of spontaneous bioconversion of tamarind seeds increased the digestibility of tamarind seed nutrients and it was concluded that the best time of spontaneous bioconversion was for 72 hours.

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Optimasi Waktu Inkubasi Lactobacillus rhamnosus SKG 34 Dalam Produksi Enzim Penggumpal Susu

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2020.v09.i02.p01 ◽

2020 ◽

Vol 9 (2) ◽

pp. 108

Author(s):

Wulandari Setiadarma ◽

Dewa Gede Mayun Permana ◽

Komang Ayu Nocianitri

Keyword(s):

Incubation Time ◽

Protease Activity ◽

Block Design ◽

Lactobacillus Rhamnosus ◽

Duncan Multiple Range Test ◽

Milk Clotting ◽

Randomized Block Design ◽

Milk Clotting Enzyme ◽

Specific Protease ◽

Range Test

This study aims to determine the effect of incubation time on milk clotting enzyme (MCE) activity produced by L. rhamnosus SKG 34 and determine the optimum incubation time to produce the highest its activity. This study used a randomized block design (RBD) with incubation time as a treatment consisting of 8 levels, that were 6 hours, 12 hours, 18 hours, 24 hours, 30 hours, 36 hours, 42 hours, and 48 hours. Data were analyzed with Variance Analysis (ANOVA) than followed by Duncan Multiple Range Test (DMRT). The analyzed were repeated 3 times resulting in 24 experimental units. The results showed that the incubation time significantly affected protease activity, MCE activity, specific protease activity and ratio of MCE to protease but did not affect the total LAB. The optimum incubation time of L. rhamnosus SKG 34 is 12 hours with total LAB 1.83 x 109 CFU/ml and protease activity 180.67 U/ml, MCE activity 595.06 SU, protease spesific activity 73.149 U/mg and ratio of MCE to protease 3.29 SU/U. Keywords : incubation time, Enzyme protease, Lactobacillus rhamnosus SKG 34, Milk clotting enzyme.

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PENGARUH HERBISIDA BERBAHAN AKTIF PARAQUAT TERHADAP PERSENTASE KEMATIAN GULMA DAN JUMLAH MIKROORGANISME TANAH

Jurnal Agro Estate ◽

10.47199/jae.v4i2.182 ◽

2020 ◽

Vol 4 (2) ◽

pp. 99-108

Author(s):

Sakiah Sakiah ◽

Guntoro Guntoro ◽

Adri Moses Manullang

Keyword(s):

Soil Microorganisms ◽

Block Design ◽

Mimosa Pudica ◽

Duncan Multiple Range Test ◽

Ageratum Conyzoides ◽

Randomized Block Design ◽

Range Test

Paraquat is an active herbicide used to control weeds chemically. This research aim is to determine the effect of herbicide applications with active paraquat matters on the percentage of weed mortality and the number of soil microorganisms. This research used a non-factorial randomized block design with five levels of treatment, that is P0: control; P1: 5 ml of paraquat in 1 litre of water, frequency of application is once a week; P2: 10 ml of paraquat in 1 litre of water, frequency of application is once a week; P3: 10 ml of paraquat in 1 litre of water, frequency of application is once in two weeks; and P4: 5 ml of paraquat in 1 litre of water, frequency of application is once in two weeks. The results of the observations were compiled in a Variety Checklist and continued with the Duncan Multiple Range Test. The results showed that the dominant weeds in the research plot were Ageratum conyzoides, Mimosa pudica, and Paspalum commersonii.The application of 5 ml/l paraquat herbicide, the frequency of application once a week was effective in reducing weed mortality. However, the use of paraquat did not significantly affect the number of soil microorganisms

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