scholarly journals Laser-Induced Spectral-Selective Autofluorescent Microscopy as a Prospective Method of Research in Biomedicine

2020 ◽  
Vol 27 (4) ◽  
pp. E202048
Author(s):  
Nataliia Kozan ◽  
Ivan Savka ◽  
Viktor Kryvetskyi ◽  
Igor Oliynyk
Keyword(s):  
Diagnostic Error ◽  
Medical Diagnostics ◽  
Optical Biopsy ◽  
Optical Methods ◽  
Additional Information ◽  
The Past ◽  
Endogenous Fluorophores ◽  
Functional Features ◽  
Living Tissues ◽  
Analytical Tools

In modern medical diagnostics, optical methods of studying living tissues have become widespread and are collectively called "optical biopsy". One such method is autofluorescence microscopy, which provides additional information about the structural and functional features of the sample. In this paper, an analysis of existing data was performed on the properties of autofluorescence of cells and tissues to evaluate the available instrumental systems and methods for monitoring autofluorescence and the potential for its application in the biomedical field. Over the past few years, advanced optical-electronic methods have become available to detect various pathological conditions of tissues and environments of the human body by evaluating signals emitted by endogenous fluorophores. Because these molecules are often involved in basic biological processes, they are important parameters for checking the condition of cells and tissues. In our opinion, analytical methods based on autofluorescence monitoring have great potential in both research and diagnosis, and interest in the use of these new analytical tools is constantly growing. Methods based on autofluorescence can give more information about the object under study with relatively lower costs and less diagnostic error.

scholarly journals Spontaneous Raman spectroscopy for intracranial tumors diagnostics ex vivo

2020 ◽  
Vol 9 (3) ◽  
pp. 4-12
Author(s):  
I. D. Romanishkin ◽  
L. R. Bikmukhametova ◽  
T. A. Savelieva ◽  
S. A. Goryaynov ◽  
A. V. Kosyrkova ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectra ◽  
Ex Vivo ◽  
Principal Component ◽  
Intraoperative Navigation ◽  
Optical Biopsy ◽  
Optical Methods ◽  
Intracranial Tumors ◽  
Tissue Samples ◽  
Endogenous Fluorophores

Neurosurgery of intracranial tumors, especially of glial origin, is a non-trivial task due to their infiltrative growth. In recent years, optical methods of intraoperative navigation have been actively used in neurosurgery. However, one of the most widely used approaches based on the selective accumulation of fluorescent contrast medium (5-ALA-induced protoporphyrin IX) by the tumor cannot be applied to a significant number of tumors due to its low accumulation. On the contrary, Raman spectroscopy, which allows analyzing the molecular composition of tissues while preserving all the advantages of the method of fluorescence spectroscopy, does not require the use of an exogenous dye and may become a method of choice when composing a system for intraoperative navigation or optical biopsy. This work presents the first results of using the principal component method to classify Raman spectra of human glioblastoma with intermediate processing of spectra to minimize possible errors from the fluorescence of both endogenous fluorophores and photosensitizers used in fluorescence navigation. As a result, differences were found in the principal component space, corresponding to tissue samples with microcystic components, extensive areas of necrosis, and foci of fresh hemorrhages. It is shown that this approach can serve as the basis for constructing a system for automatic intraoperative tissue classification based on the analysis of Raman spectra.

scholarly journals The emerging technology of biohybrid micro-robots: a review

2021 ◽  
Author(s):  
Zening Lin ◽  
Tao Jiang ◽  
Jianzhong Shang
Keyword(s):  
Self Assembly ◽  
Degrees Of Freedom ◽  
High Energy ◽  
Living Cells ◽  
Multiple Degrees Of Freedom ◽  
The Past ◽  
Great Prevalence ◽  
Performance Decline ◽  
High Energy Efficiency ◽  
Living Tissues

Abstract In the past few decades, robotics research has witnessed an increasingly high interest in miniaturized, intelligent, and integrated robots. The imperative component of a robot is the actuator that determines its performance. Although traditional rigid drives such as motors and gas engines have shown great prevalence in most macroscale circumstances, the reduction of these drives to the millimeter or even lower scale results in a significant increase in manufacturing difficulty accompanied by a remarkable performance decline. Biohybrid robots driven by living cells can be a potential solution to overcome these drawbacks by benefiting from the intrinsic microscale self-assembly of living tissues and high energy efficiency, which, among other unprecedented properties, also feature flexibility, self-repair, and even multiple degrees of freedom. This paper systematically reviews the development of biohybrid robots. First, the development of biological flexible drivers is introduced while emphasizing on their advantages over traditional drivers. Second, up-to-date works regarding biohybrid robots are reviewed in detail from three aspects: biological driving sources, actuator materials, and structures with associated control methodologies. Finally, the potential future applications and major challenges of biohybrid robots are explored. Graphic abstract

scholarly journals Protein L—More Than Just an Affinity Ligand

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 874
Author(s):  
Stefan Kittler ◽  
Mihail Besleaga ◽  
Julian Ebner ◽  
Oliver Spadiut
Keyword(s):  
Antibody Fragments ◽  
Downstream Process ◽  
Affinity Ligands ◽  
The Past ◽  
Affinity Ligand ◽  
Recombinant Production ◽  
Production Processes ◽  
Monitoring Tools ◽  
Platform Technology ◽  
Analytical Tools

In the past 30 years, highly specific drugs, known as antibodies, have conquered the biopharmaceutical market. In addition to monoclonal antibodies (mAbs), antibody fragments are successfully applied. However, recombinant production faces challenges. Process analytical tools for monitoring and controlling production processes are scarce and time-intensive. In the downstream process (DSP), affinity ligands are established as the primary and most important step, while the application of other methods is challenging. The use of these affinity ligands as monitoring tools would enable a platform technology to monitor process steps in the USP and DSP. In this review, we highlight the current applications of affinity ligands (proteins A, G, and L) and discuss further applications as process analytical tools.

scholarly journals Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

2021 ◽  
Author(s):  
Jijo Lukose ◽  
Sanoop Pavithran M. ◽  
Mithun N. ◽  
Ajaya Kumar Barik ◽  
Keerthilatha M. Pai ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Viral Infections ◽  
Clinical Specimen ◽  
Human Saliva ◽  
Medical Diagnostics ◽  
Optical Methods ◽  
Health Conditions ◽  
Personal Health ◽  
Fast Detection ◽  
Non Invasive

AbstractHuman saliva can be treated as a pool of biological markers able to reflect on the state of personal health. Recent years have witnessed an increase in the use of optical devices for the analysis of body fluids. Several groups have carried out studies investigating the potential of saliva as a non-invasive and reliable clinical specimen for use in medical diagnostics. This brief review aims to highlight the optical technologies, mainly surface plasmon resonance (SPR), Raman, and Fourier transform infrared (FTIR) spectroscopy, which are being used for the probing of saliva for diverse biomedical applications. Advances in bio photonics offer the promise of unambiguous, objective and fast detection of abnormal health conditions and viral infections (such as COVID-19) from the analysis of saliva.

If things go South: The renewed policy of sport mega events allocation and its implications for future research

2021 ◽  
pp. 101269022098134
Author(s):  
Billy Graeff ◽  
Jorge Knijnik
Keyword(s):  
21St Century ◽  
Public Expenditure ◽  
Global South ◽  
International Olympic Committee ◽  
Future Research ◽  
Construction Costs ◽  
Mega Events ◽  
The Past ◽  
Global North ◽  
Analytical Tools

The past few decades have seen an increase of sport mega events (SMEs) held outside the Global North. This tendency has been accompanied by a growing public expenditure in these events. This paper employs selected Global South SMEs to discuss this trend. By critically analysing public documents, biddings and reports, the study traces comparisons between 21st-century Global South and Global North SMEs expenditures, in the revenue of franchise owners (FIFA and the International Olympic Committee), in construction costs within the budgets and in the costs related to security. This comprehensive and intertwined investigation shows the need for new analytical tools – such as the Renewed Policy of Sport Mega Events Allocation, a concept developed here - to better capture the central questions posed by the challenges of ‘SMEs going South’.

Statistics and Economic History

1941 ◽  
Vol 1 (1) ◽  
pp. 26-41 ◽  
Author(s):  
Simon Kuznets
Keyword(s):  
Statistical Analysis ◽  
Economic History ◽  
Raw Materials ◽  
The Other ◽  
Economic Study ◽  
Statistical Research ◽  
Historical Approach ◽  
The Past ◽  
Economic Inquiry ◽  
Analytical Tools

This paper deals with the relation between statistical analysis as applied in economic inquiry and history as written or interpreted by economic historians. Although both these branches of economic study derive from the same body of raw materials of inquiry—the recordable past and present of economic society—each has developed in comparative isolation from the other. Statistical economists have failed to utilize adequately the contributions that economic historians have made to our knowledge of the past; and historians have rarely employed either the analytical tools or the basic theoretical hypotheses of statistical research. It is the thesis of this essay that such failure to effect a close interrelation between historical approach and statistical analysis needs to be corrected in the light of the final goal of economic study.

scholarly journals An Efficient Multicolor Two-Photon Imaging of Endogenous Fluorophores in Living Tissues by Wavelength Mixing

2017 ◽  
Vol 112 (3) ◽  
pp. 186a ◽  
Author(s):  
Chiara Stringari ◽  
Lamiae Abdeladim ◽  
Guy Malkinson ◽  
Willy Supatto ◽  
Sébastien Brizion ◽  
...  
Keyword(s):  
Two Photon ◽  
Endogenous Fluorophores ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Living Tissues

scholarly journals Periodicity: Interpreting Waves of Information in Osundare’s Harvestcall

2017 ◽  
Vol 10 ◽  
pp. 42-70
Author(s):  
Taofeek Olaiwola Dalamu
Keyword(s):  
Time Frame ◽  
The Past ◽  
Systemic Analysis ◽  
Literary Device ◽  
Different Shapes ◽  
Analytical Tools ◽  
The Waves

The study investigated the waves of information of Osundara’s Harvestcall to show textual movements as layered by the poet. To achieve that objective, the poem of about 76 lines was collapsed into 44 clauses as a tradition that paves a way for systemic analysis of texts in different shapes, sizes and constructs. Halliday’s Theme and Rheme served as analytical tools that processed the text after which the investigation utilized tables and graphs as indicators of waves of information of Harvestcall. The analysis reveals three separate waves of the text, namely: (i) sectional organization – perceives Sections I, II and III are the Theme while Section IV is the Rheme; (ii) clause constructs – demonstrates multiple Themes that flow to rhematic structures; and (iii) time frame exposition – espouses the past farming commitment as Theme and its current neglect as Rheme. In addition, observation shows waves interference. That is, the switching of Theme 2 and Subject Theme in Sections I, II and III for Subject Theme and Theme 2 respectively in Section IV. As linguistic concepts can reveal so much meanings of a literary device, the study suggests their applications across the board of genres of literature.

scholarly journals Innovative Strategy for Counterfeit Analysis

2017 ◽  
Vol 1 ◽  
pp. maapoc.0000013 ◽  
Author(s):  
Klara Dégardin ◽  
Yves Roggo
Keyword(s):  
Chemical Composition ◽  
Law Enforcement ◽  
Near Infrared ◽  
Analytical Data ◽  
Analytical Strategy ◽  
Innovative Strategy ◽  
X Ray ◽  
Additional Information ◽  
Counterfeit Medicine ◽  
Analytical Tools

Currently, counterfeit medicine is a significant issue for the pharmaceutical world, and it targets all types of therapeutic areas. The health consequences are appalling, since counterfeit medicines can contain impurities and the wrong chemical composition, and can be manufactured and/or stored in dreadful conditions. The provision of fast and reliable analytical tools can contribute to an efficient fight against this phenomenon. In this paper, an analytical strategy based on mobile and forensic laboratories is presented. The mobile equipment, composed of handheld x-ray fluorescence, Raman, infrared, and near-infrared spectrometers, and a handheld microscope, can be used as a first screening tool to detect counterfeits. The counterfeits can then be confirmed in a forensic-dedicated lab in which the chemical composition of the counterfeits is determined to evaluate the danger encountered by the patients. Relevant links with former counterfeit cases then can be revealed based on the analytical data, and can be interpreted from a forensic intelligence perspective in order to provide additional information for law enforcement.

Piramal Diagnostics

2011 ◽  
Vol 15 (01) ◽  
pp. 97-121
Author(s):  
Neeraj Dwivedi ◽  
Arvinder Singh
Keyword(s):  
Strategic Planning ◽  
Structural Characteristics ◽  
Vice President ◽  
Medical Diagnostics ◽  
Growth Strategy ◽  
Decision Situation ◽  
The Past ◽  
Strategic Option ◽  
The Future ◽  
Future Growth

The case presents a decision situation facing the Vice President of strategic planning at Piramal Diagnostics Limited, who has to formulate the future growth strategy and decide on the roadmap. The company is the largest player in the organized medical diagnostics industry in India and has shown attractive growth in the past few years. The case describes the structural characteristics of the medical diagnostics industry in India and follows it with a description of the strengths and weaknesses of Piramal Diagnostics and the strategies adopted by it. The Vice President is expected to choose an appropriate strategic option to help the company achieve its ambitious growth target.

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