Blood Biochemical, Hormonal and Mineral Status of Cyclic, Acyclic, Endometriotic and Pregnant Crossbred Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
Chirag I Patel ◽  
A J Dhami ◽  
M T Panchal ◽  
N P Sarvaiya ◽  
S V Shah
Keyword(s):  
Total Protein ◽  
Clinical Status ◽  
Inorganic Phosphorus ◽  
Late Gestation ◽  
Plasma Progesterone ◽  
Crossbred Cows ◽  
Blood Biochemical ◽  
Cholesterol Levels ◽  
Three Stages ◽  
The Mean

A study was carried out on infertile (acyclic and endometriotic) crossbred cows under field and normal cyclic (all 4 phases of cycle) as well as pregnant (3, 6, 9 month) crossbred cows of University farm to evaluate the plasma progesterone (P4) and estradiol (E2) hormones by RIA, and plasma total protein, cholesterol, calcium and phosphorus concentrations by using assay kits on chemistry analyzer. The mean progesterone levels in cows during the diestrus phase and in pregnancy were significantly (p less than 0.05) higher than those during proestrus, estrus, metestrus, anestrus, and endometritis status. At six month of gestation, the mean P4 level was significantly (p less than 0.05) higher than at early or late gestation. The mean E2 values at estrus and 9th month of gestation were highest (p less than 0.01) as compared to another status. The mean plasma total protein and cholesterol levels were significantly (p less than 0.05) lower during six and nine months of pregnancy than during cyclic and acyclic stages. The cholesterol profile of all three stages of pregnancy and endometriotic cows were statistically similar, though distinctly low at 9 month of pregnancy. Plasma levels of P4 and E2 thus correlated with the physiological and clinical status of cows, while cholesterol levels reflected steroidogenic status. The mean plasma calcium and inorganic phosphorus concentrations of cyclic, acyclic, pregnant and endometriotic cows, however, did not differ significantly.

scholarly journals Effect of Rumen Mega Mineral Bolus Insertion at Calving on Blood Biochemical and Minerals Profile and Postpartum Fertility in Kankrej Cows

2020 ◽  
Vol 16 (01) ◽  
pp. 01-06
Author(s):  
Mehrajuddin Naikoo ◽  
Arjun J Dhami ◽  
Bhupendra C Parmar
Keyword(s):  
Inorganic Phosphorus ◽  
Valid Conclusion ◽  
Plasma Progesterone ◽  
Mean Values ◽  
Blood Biochemical ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Rumen Bolus ◽  
The University

A study was conducted on 12 pluriparous parturient Kankrej cows of the University Farm, in Anand, randomly divided into two equal groups (n = 6 each). Group-I cows were administered on the day of calving with a sustained release 80 g mega mineral rumen bolus (Prepavel® 1 bolus/animal, Neolait, France) intra-ruminal through a specially designed applicator, while group-II animals served as untreated control. The animals were monitored periodically from the day of calving till 140 days postpartum along with other herd mates. Blood samples were obtained at 10 days interval in heparinized vacutainers for plasma progesterone, biochemical, and macro-micro mineral profiling. Among the six Kankrej cows of group-I, only one animal exhibited prominent estrus signs on day 96 postpartum and conceived at first service (AI) giving CR of 16.66%, while in group-II three cows exhibited estrus between day 73 and 86 postpartum, and conceived with one or two AI giving CR of 50% by 150 days postpartum. In both the groups, all other cows remained subestrus for more than 150 days postpartum and conceived very late, though mostly with single service at spontaneous estrus. The mean values of plasma progesterone (0.74 ± 0.32 to 5.57 ± 0.78 ng/mL), total cholesterol (103.88 ± 3.20 to 237.17 ± 24.66 mg/dL) and triglycerides (17.07 ± 1.16 to 28.29 ± 1.75 mg/dL) differed significantly (p less than 0.01) between postpartum intervals in both the groups, but not between groups at any of the intervals. The values of plasma total protein, calcium, inorganic phosphorus, and magnesium as well as trace elements, viz., zinc, iron, copper, cobalt, and manganese, however varied insignificantly and inconsistently between intervals and also between groups from day 0 to day 140 postpartum. Thus, the insertion of Mega mineral ruminal bolus on the day of calving did not influence the plasma profile of biochemical/ metabolic constituents and macro-micro minerals profile in lactating postpartum Kankrej cows and was not beneficial in improving postpartum fertility. However, further study on a larger sample size is required to draw a valid conclusion.

Ovarian Dynamics, Plasma Endocrine Profile and Fertility Response Following Synchronization Protocols in Crossbred Cows with Cystic Ovaries

2020 ◽  
Author(s):  
A. J. Dhami ◽  
J. H. Chauhan ◽  
K. K. Hadiya ◽  
N. P. Sarvaiya
Keyword(s):  
Estrogenic Activity ◽  
Treatment Modalities ◽  
Ovarian Cysts ◽  
Control Group ◽  
Therapeutic Success ◽  
Plasma Progesterone ◽  
Lh Surge ◽  
Crossbred Cows ◽  
Conception Rates ◽  
The Mean

Background: Cystic ovarian degeneration (COD) with prevalence of 10-13% is a common and economically important condition in dairy cattle affecting fertility. Predisposing factors and aetiology of ovarian cysts are multifaceted. The most widely accepted cause is either absent, insufficient or wrong time occurrence of pre-ovulatory LH-surge. The accuracy of differential diagnosis of cyst per rectum can be increased by simultaneous use of USG and/or plasma progesterone assay and therapeutic success depends on type and duration of cystic condition. The literature on these aspects in crossbred cows is meagre, hence was planned to differentially diagnose the ovarian follicular and luteal cysts based on clinical, sonographic and endocrine findings and to study the estrus response and conception rates following different treatment modalities of COD in crossbred cattle. Methods: This study was conducted on greater than 90 days postpartum HF crossbred cows with cystic ovaries (n=58) confirmed by per rectal palpation and ultrasonography. The animals with follicular cysts were randomly treated with either conventional Ovsynch or Ovsynch + CIDR protocol (n=10 each) and those with luteal cysts with either Double PG injections 11 days apart or modified Ovsynch protocol (n=16 each) with fixed time AI. A group of six cystic cows was kept as untreated control. Blood samples were collected in heparinized vacutainers, together with trans-rectal ultrasonography, on day 0 (just before initiation of treatment), on day of last PGF2á injection, on day of induced estrus/FTAI and on day 12 post-AI for determination of plasma progesterone and estradiol-17â by RIA technique. Results: The mean diameters and wall thickness of ovarian cysts regressed significantly (p less than 0.01) among responded cows of all four protocols. The estrus induction response within 48-96 hrs of last PG injection with Ovsynch, Ovsynch + CIDR, Modified Ovsynch and Double PG protocols was 90.00, 100.00, 87.50 and 81.25 %, respectively. The conception rates at induced estrus with FTAI for these protocols were 50.00, 40.00, 50.00 and 43.75 %, respectively and the corresponding overall conception rates for 3 cycles’ post-treatment were 60.00, 70.00, 68.75 and 56.25 %, respectively, compared to zero result of control group. The mean plasma progesterone concentration varied highly significantly (p less than 0.01) between periods of treatment in all protocols, the values were lower (p less than 0.01) on day 0 and on day of FTAI than on the day of last PG injection and on day 12 post-AI. The values on day 12 post-AI were significantly higher in conceived than non-conceived cows. The trend of estradiol-17â profile on day of last PG injection was reversed than on day 0. The higher levels of estradiol-17â on day of FTAI were associated with regression of cystic structures and development of new dominant follicles with estrogenic activity concomitant to induced estrus and ovulatory LH surge in most of the animals as evident from USG monitoring, behavioural signs and conception rates.

scholarly journals STUDY ON SOME HEMATOLOGICAL AND BLOOD BIOCHEMICAL PARAMETERS OF NEWLY INTRODUCED LAYER BREEDS DEPENDING ON THE LAYING PERIOD

2020 ◽  
Vol 18 (Suppl.1) ◽  
pp. 11-18
Author(s):  
A. Arnaudov ◽  
A. Bochukov ◽  
P. Petrov ◽  
V. Gerzilov ◽  
D. Penkov
Keyword(s):  
Total Cholesterol ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Blood Parameters ◽  
Egg Laying ◽  
Total Protein Content ◽  
Blood Biochemical ◽  
Cholesterol Levels ◽  
Blood Glucose Test ◽  
Glucose Test

PURPOSE: To compare some major hematological and biochemical blood parameters of 3 newly introduced poultry breeds – Australorp, Marans and Araucana, during different stages of their egg-laying period. METHODS: Five blood parameters were investigated: hematocrit, hemoglobin, glucose, total protein and total cholesterol. A hundred blood samples were tested, collected at three laying stages – beginning, middle and end. RESULTS: Statistically significant differences in the hemoglobin levels by sexes were established: in Marans breed (males – between 8.38 and 13.45; females – between 7.30 and 9.02 g*dl-1) and Araucana (males – between 9.17 and 13.65; females – between 9.08 and 9.40 g*dl-1). The hematocrit levels in the males (from 23.16% in Marans to 40.88% in Australorp) were significantly higher than the levels in the females (from 23.72% in Marans to 30.48% in Australorp). The blood glucose test of the cocks (from 32.07 in Marans to 40.88 mmol*l-1 in Australorp) showed higher levels compared to the female (from 23.72 in Marans to 30.48 mmol*l-1 in Australorp). The total protein content in the layers’ blood (between 5.97 in Araucana and 8.73 g*dl-1 in Marans) was higher compared to cocks. The total cholesterol levels were, as follows: males – from 2.05 in Araucana to 3.49 mmol*l-1 in Australorp; females – from 1.92 in Australorp to 6.57 3.49 mmol*l-1 in Araucana. CONCLUSIONS: The variations in the investigated parameters in the blood are a result of the simultaneous effect of several factors - breed genetic differences, differences in egg-laying effort and degree of adaptation.

scholarly journals Observations on the Activities of Certain Serum Enzymes and Levels of Some Biochemical Parameters in Spent Horses with Liver Damage

2018 ◽  
Vol 10 (3) ◽  
pp. 305-309
Author(s):  
Onyinyechukwu Ada AGINA ◽  
John Ikechukwu IHEDIOHA
Keyword(s):  
Liver Damage ◽  
Total Protein ◽  
Total Bilirubin ◽  
Serum Biochemistry ◽  
Chronic Liver ◽  
Acute Liver Damage ◽  
Chronic Liver Damage ◽  
Cholesterol Levels ◽  
The Mean ◽  
Group 2

Clinical biochemistry tests are one of the tests designed to assess the functional status of the liver in domestic animals and man. This study evaluated the activities of some serum enzymes and the level of some biochemical parameters in horses with acute and chronic liver damage. This study was carried out in Nigerian horses presented for sale at the Obollo Afor horse depot, Udenu Local Government Area, Enugu State Nigeria. A total of 100 adult Nigerian horses of either sex were studied once a week for a period of 24 weeks. Serum biochemistry parameters indicated for the assessment of liver function were evaluated following standard procedures. These parameters include: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total proteins, albumin, globulin, total cholesterol, total bilirubin and urea. Based on the outcome of the serum biochemistry evaluation, the horses were assigned into 3 groups namely: apparently healthy horses (Group 1), horses with biochemical markers of acute liver damage (Group 2) and horses with biochemical markers of chronic liver damage (Group 3). The mean serum ALT, AST, ALP and total bilirubin values of group 2 horses were significantly (p < 0.05) higher than those of groups 1 and 3 horses. The mean serum albumin value of group 2 horses was significantly (p < 0.05) lower than that of groups 1 and 3 horses. There was no significant (p > 0.05) difference in the mean serum urea value of group 2 and 3. A significantly (p < 0.05) lower serum creatinine value was observed in group 2 horses. There were no significant (p > 0.05) differences in the mean serum total protein, globulin and cholesterol values across the groups. In the spent horses, acute liver damage was characterized by increased serum ALT, AST and ALP activities, and decrease in creatinine level, hyperbilirubinemia, slight hypoalbuminemia, normal total protein, globulin and cholesterol levels while chronic liver damage was characterized by decreased serum ALT activity, normal serum AST and ALP activities, decreased serum urea level, normal total protein, albumin, globulin, total bilirubin and cholesterol levels.

scholarly journals Serum Biochemical Profile In Normally Calved and Retained Foetal Membranes Crossbred Cows

2017 ◽  
Vol 13 (01) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
S. S. Mathur ◽  
S. P. Nema ◽  
R. Aich ◽  
...  
Keyword(s):  
Serum Calcium ◽  
Total Protein ◽  
Post Partum ◽  
Inorganic Phosphorus ◽  
Serum Glucose ◽  
Biochemical Profile ◽  
Crossbred Cows ◽  
Foetal Membranes ◽  
Serum Biochemical ◽  
Serum Biochemical Profile

Serum biochemical profile revealed that the serum calcium, inorganic phosphorus, total protein and serum glucose in cross-bred cows were significantly (P less than 0.05) lower after 12 hrs post-partum and 7th day post-partum in cows having RFM as compared to normally calved cows. Lower levels of serum calcium, inorganic phosphorus, total protein and glucose may responsible for retention of foetal membranes.

Prevalence, Risk Factors and Differential Diagnosis of Cystic Ovarian Degeneration in Crossbred Cows

2019 ◽  
Vol 15 (01) ◽  
pp. 21-25
Author(s):  
JH Chauhan1 ◽  
KK Hadiya2 ◽  
AJ Dhami
Keyword(s):  
Fixed Time ◽  
Plasma Progesterone ◽  
Crossbred Cows ◽  
Follicular Cyst ◽  
Combined Use ◽  
High Incidence ◽  
The Mean ◽  
The Right ◽  
Rectal Palpation ◽  
Follicular Cysts

The present study was conducted under field conditions on >90 days postpartum HF crossbred cows with cystic ovaries (n = 58). The diagnosis was confirmed by history and trans-rectal palpation and ultrasonographic examinations twice at weekly interval using 5.0–7.5 MHz frequency probe and was later authenticated with plasma progesterone assay and treatment response. The animals with follicular cysts were randomly treated with either conventional ovsynch or ovsynch + CIDR protocol with fixed time AI (n = 10 each), and those with luteal cysts with either double PG injections 11 days apart or modified Ovsynch protocol (n = 16 each). Among 58 cystic cows, the highest incidence (62.07 %) of the ovarian cyst was recorded in the age group of 5–7 years followed by above 7 years (36.21%) and 3–5 years (2.00%). The incidence was highest among cows of 3rd or more parity (70.69%) followed by 2nd parity (29.31%), and no case was seen in primiparous cows. Of the total 36.21% were follicular type cyst and 63.79% luteal type cysts. The right ovary had a high incidence of the cyst (51.72%) followed by the left ovary (36.21%), and bilateral (12.07%). Based on rectal palpation, the cystic ovary was classified to have follicular cyst in 36.21% (21/58) cases and luteal cysts in 63.79% (37/58). Ultrasound examination showed follicular and luteal cysts as 27.59% and 72.41%, whereas plasma P4 (less than/greator than 1 ng/mL) analysis revealed this as 20.69% and 79.31%, respectively. The clinical diagnosis became more accurate with a combination of per rectal palpation and USG and was further improved by plasma progesterone assay. The mean diameters and a wall thickness of cysts varied highly significantly (p less than 0.01) between groups/protocols. The conception rates at induced estrus with FTAI in cows under ovsynch, ovsynch + CIDR, modified ovsynch and double PG protocols were 50.00, 40.00, 50.00 and 43.75 percent, respectively. It is thus concluded that in crossbred cows luteal cysts are more common than follicular cysts, particularly in prime aged animals of 3rd or 4th parity with more of left ovarian involvement. The differentiation of cyst type is best achieved with the combined use of USG and/or plasma progesterone assay with rectal palpation, and that ovsynch protocol appeared promising for the treatment of follicular cysts and Modified Ovsynch for luteal cysts.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

Effect of High Plane of Nutrition on Blood Biochemical Profile and Onset of Puberty in Prepubertal HF X Kankrej Crossbred Heifers

2019 ◽  
Vol 15 (02) ◽  
pp. 14-17
Author(s):  
K K Hadiya ◽  
A J Dhami ◽  
D V Chaudhari ◽  
P M Lunagariya
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Plasma Levels ◽  
Age Of Onset ◽  
The Body ◽  
Monthly Interval ◽  
Control Group ◽  
Blood Biochemical ◽  
Lower Protein ◽  
The Mean

This study was initiated on 24 prepubertal Holstein x Kankrej crossbred heifers of nearly identical age (7-9 months) and body weight (130-140 kg) at University farm to evaluate the effect of high plane of nutrition on blood biochemical and minerals profile and the age at puberty. Twelve heifers were managed under routine farm feeding (control) and the rest 12 under ideal optimum feeding regime (treatment) that included extra 1 kg concentrate, 30 g min mix and ad-lib dry fodder. The body weight and ovarian ultrasonography together with blood sampling was carried out at monthly interval from 10 to 18 months of age to study the ovarian dynamics and blood biochemical changes. High plane of nutrition to growing heifers was beneficial in reducing the age of onset of puberty (by 2-3 months) compared to routine farm fed group. The mean plasma total protein and cholesterol concentrations showed a rising trend with significant variations from 10 to 16 months of age, where it got mostly stabilized indicating adult profile. The activity of enzymes GOT and GPT also rose gradually and significantly from 10 months till 14-15 months of age, and thereafter it remained more or less static till 18 months of age. The levels of both these enzymes were higher, with lower protein and cholesterol, in control than the treatment group from 15-16 months of age onwards. The mean plasma levels of both calcium and phosphorus increased gradually and significantly with advancing age till 16-17 months of age, with little higher values in supplemented than a control group. The plasma levels of zinc, iron, copper, and cobalt also showed rising trend with significant differences between 10th and 12th-14th months of age, and from 15th to 18th months of age the levels were statistically the same in all the groups with slightly higher values in the treatment group.

Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Jugular Vein ◽  
Dairy Farm ◽  
Fetal Membranes ◽  
Monocyte Count ◽  
Blood Samples ◽  
Mean Values ◽  
Crossbred Cows ◽  
Haematological Profile ◽  
The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

Pattern of plasma progesterone, oestradiol-17β, luteinizing hormone and androgen in non-pregnant buffalo (Bubalus bubalis)

1982 ◽  
Vol 100 (2) ◽  
pp. 279-284 ◽  
Author(s):  
R. C. Arora ◽  
R. S. Pandey
Keyword(s):  
Luteinizing Hormone ◽  
Peak Level ◽  
Systemic Circulation ◽  
Bubalus Bubalis ◽  
High Concentration ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Androgen Level ◽  
The Mean ◽  
Low Levels

Abstract. Domestic buffaloes were used to characterize the pattern of progesterone, oestradiol-17β, LH and androgen in the systemic circulation following infertile insemination. Concentrations of hormones were measured by RIA in blood samples collected daily or at alternate days following insemination. The concentration of progesterone was lowest on the day of insemination, and increased significantly to a peak level of 4.00 ± 0.60 ng/ml by day 13 post insemination. After day 17, it declined significantly (P < 0.01) to reach low levels by day 21. The concentration of oestradiol-17β was high at the time of insemination and declined significantly (P < 0.01) by day 2 after insemination. It was maintained around the basal level till day 18 with minor peaks in between this period. It again rose significantly (P < 0.01) at subsequent oestrus. The mean level of LH was highest at the time of insemination, and declined significantly (P < 0.01) by day 1 post insemination. It did not vary appreciably till the animal returned to oestrus. The oestrous value of LH and progesterone were negatively correlated (r = −0.77). The androgen level was observed to be high at insemination in 3 out of 5 animals, but the overall pattern of this steroid was inconsistent during the period studied. A high concentration of androgen was recorded in all the animals from day 2–5 before the onset of oestrus.

Sign in / Sign up

Export Citation Format

Share Document