scholarly journals STUDY ON SOME HEMATOLOGICAL AND BLOOD BIOCHEMICAL PARAMETERS OF NEWLY INTRODUCED LAYER BREEDS DEPENDING ON THE LAYING PERIOD

2020 ◽  
Vol 18 (Suppl.1) ◽  
pp. 11-18
Author(s):  
A. Arnaudov ◽  
A. Bochukov ◽  
P. Petrov ◽  
V. Gerzilov ◽  
D. Penkov

PURPOSE: To compare some major hematological and biochemical blood parameters of 3 newly introduced poultry breeds – Australorp, Marans and Araucana, during different stages of their egg-laying period. METHODS: Five blood parameters were investigated: hematocrit, hemoglobin, glucose, total protein and total cholesterol. A hundred blood samples were tested, collected at three laying stages – beginning, middle and end. RESULTS: Statistically significant differences in the hemoglobin levels by sexes were established: in Marans breed (males – between 8.38 and 13.45; females – between 7.30 and 9.02 g*dl-1) and Araucana (males – between 9.17 and 13.65; females – between 9.08 and 9.40 g*dl-1). The hematocrit levels in the males (from 23.16% in Marans to 40.88% in Australorp) were significantly higher than the levels in the females (from 23.72% in Marans to 30.48% in Australorp). The blood glucose test of the cocks (from 32.07 in Marans to 40.88 mmol*l-1 in Australorp) showed higher levels compared to the female (from 23.72 in Marans to 30.48 mmol*l-1 in Australorp). The total protein content in the layers’ blood (between 5.97 in Araucana and 8.73 g*dl-1 in Marans) was higher compared to cocks. The total cholesterol levels were, as follows: males – from 2.05 in Araucana to 3.49 mmol*l-1 in Australorp; females – from 1.92 in Australorp to 6.57 3.49 mmol*l-1 in Araucana. CONCLUSIONS: The variations in the investigated parameters in the blood are a result of the simultaneous effect of several factors - breed genetic differences, differences in egg-laying effort and degree of adaptation.

2019 ◽  
Vol 17 (4) ◽  
pp. 323-328
Author(s):  
D. Penkov ◽  
A. Arnaudov ◽  
M. Nikolova

A monitoring study of some main blood parameters of a flock of free-range guinea fowls has been conducted. The following results (depending on sex and laying intensity) have been established: hemoglobin content – between 145.8 (males - before peak of laying intensity) and 98 g/L (females - before the peak of laying intensity), hematocrit – between 43.8 (males – before the peak of laying intensity) and 28.8% (females – before the peak of laying intensity), glucose – between 11.7 (males – before the peak of laying intensity) and 8.24 mmol/L (females – end of the laying period) and total protein content – between 5.86 (females – during the peak of laying intensity) and 3.30 g/dL (males - during the peak of laying intensity).


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1810
Author(s):  
Paul Uiuiu ◽  
Călin Lațiu ◽  
Tudor Păpuc ◽  
Cristina Craioveanu ◽  
Andrada Ihuț ◽  
...  

Blood biochemistry parameters are valuable tools for monitoring fish health. Their baseline values are still undefined for a multitude of farmed fish species. In this study, changes in the blood profile of rainbow trout females (Oncorhynchus mykiss) from three farms were investigated using different biomarkers during the summer season. In the given context, the main water physicochemical parameters were investigated and twelve biochemical parameters were measured from blood samples of rainbow trout reared in the Fiad, Șoimul de Jos, and Strâmba farms. We selected these farms because the genetic background of the rainbow trout is the same, with all studied specimens coming from the Fiad farm, which has an incubation station. Forty-five samples were collected monthly (May to August) throughout summer to observe the changes in the blood profile of rainbow trout. Principal component analysis showed a clear separation both among the studied farms and months. Furthermore, significant correlations (p < 0.05) between the majority of the biochemical parameters were found, indicating that the environmental parameters can influence several blood parameters at the same time. The present study provides several useful norms for assessing the welfare of rainbow trout, indicating that the relationships among different parameters are important factors in interpreting the blood biochemical profiles.


2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.


Author(s):  
Cristian Ovidiu COROIAN ◽  
Vioara MIRESAN ◽  
Aurelia COROIAN ◽  
Camelia RADUCU ◽  
Luisa ANDRONIE ◽  
...  

The health status of cows is evaluated and depending on haematological and biochemical profile of blood. Nutrition is the main technological factor that can produce profound changes in the metabolic profile in animals (Dhiman et al., 1991; Khaled et al., 1999; Ingvartsen, 2006). Blood parameters analyze can lead to identify if there are errors in nutrition of lactating cows (Payne et al., 1970). The aim of this study was the evaluation of metabolic and biochemical changes that occur during colostrum period and in terms of number of lactations in cows. The biological material was represented by a total of 60 heads of dairy cows from a family farm from Sălaj County, Romania. The cows are all from Holstein breed and presented no clinical signs of any specific pathology. Blood samples were collected from the jugular vein of each cow and analyzed. 10 individuals from each of the six lactations have been randomly selected. Haematological and biochemical parameters showed variations depending on factors analyzed here. In lactation 1 Hb was 7.55±3.05 (g/dl), while in lactation 6 the value was 12.5±2.10 (g/dl). RBC ranged as follows: in lactation 1 - 28.50±2.05 and in lactation 6 - 30.02±2.05. Lymphocytes varied within very wide limits under the influence of lactation: in lactation 1 - 2.8±1.56 and in lactation 6 - 7.55±1.80. The number of lactations and lactation rank have influenced blood biochemical and hematological parameters in dairy cows. Biochemical parameters are influenced by post-partum day, showing the lowest values in the early days of colostral period and the highest in the last few days of the same period.


2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Petra Hrabčáková ◽  
Eva Voslářová ◽  
Iveta Bedáňová ◽  
Vladimíra Pištěková ◽  
Jan Chloupek ◽  
...  

The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P<0.05) in haematocrit, erythrocytes, and haemoglobin values, whereas monocytes, eosinophils, the heterophil/lymphocyte ratio, phosphorus, and calcium exhibited an increase (P<0.05). At the end of the laying period, an increase (P<0.05) was recorded in the count of leukocytes, heterophils, lymphocytes and basophils, the heterophil to lymphocyte ratio, and the concentrations of aspartate aminotransferase, cholesterol, phosphorus, and calcium, whereas lower values (P<0.05) were recorded for haematocrit and plasma total protein in comparison with the values of the indicators at the beginning of the laying period. The results provide new information about dynamic changes in selected haematological and biochemical parameters in clinically healthy common pheasant hens during the laying period.


2014 ◽  
Vol 17 (1) ◽  
pp. 47-52 ◽  
Author(s):  
J. Schumann ◽  
I. Bedanova ◽  
E. Voslarova ◽  
P. Hrabcakova ◽  
J. Chloupek ◽  
...  

Abstract The present paper provides new experimental data on the biochemical and haematological profile of blood in pheasant hens, and points out the changes in both biochemical and haematological parameters that occur during the laying period. Significant effects of egg laying on both the biochemical and the haematological blood parameters of pheasant hens were found. Biochemical analyses revealed a significant increase in the metabolites cholesterol, uric acid, lactate, the enzyme aspartate aminotransferase (AST) and the minerals calcium and phosphorous, as well as a significant decrease in total protein, albumin and glucose in the course of the laying period. Haematological analyses revealed a significant increase in the count of leukocytes, lymphocytes, eosinophils, basophils and monocytes due to egg laying. In addition, the erythrocyte count and haemoglobin content significantly decreased in the middle of the laying period and then rebounded at the end of the laying period. The haematocrit content gradually decreased till the end of the laying period. All together, the results of this study underline the impact of the reproduction status of pheasant hens on basic blood parameters. The biochemical and haematological values presented in this study may be of help in assessing disease conditions in laying pheasant hens.


2021 ◽  
Vol 23 (101) ◽  
pp. 21-25
Author(s):  
L. V. Koreyba

The success of the control of obstetric diseases in cattle depends on the timely implementation of preventive measures, and, in cases of pathology, on diagnosis, effective obstetric care and treatment. A prominent place is given to blood tests among the methods that allow to objectively assess the state of health and the course of the pathological process in animals' bodies. The study of biochemical parameters of blood allows for the assessment of the state of metabolic processes in animals, with high accuracy in establishing the body's general condition and predicting complications and adjusting their prevention and therapy. The goal of the work: our work aimed to study the biochemical parameters of the blood of deep-calving heifers for the prediction, correction, and prevention of obstetric pathology during childbirth and puerperium. The research was carried out on deep-calving heifers of Holstein breed selected on the principle of analogues. Biochemical examination of blood serum was performed in the district laboratory of veterinary medicine. Indicators of protein and mineral metabolism were determined in the blood serum of deep- calving heifers: total protein, carotene, reserve alkalinity, inorganic phosphorus, albumin content, class M immunoglobulins. The obtained research results indicate that the total protein content corresponds to the norm in deep-calving heifers of the Holstein black-spotted breed. The protein coefficient exceeds the standard. There are pronounced deviations in the blood's biochemical parameters: low levels of carotene almost two-fold, reserve alkalinity – 1.91-fold, the total calcium content – by 16.08 % and the ratio of calcium to phosphorus by 17.78 %. Since the decrease in carotene content correlates with the predisposition to endometritis, the Holstein black-spotted breed's deep-calving heifers are prone to developing birth and postpartum pathology. For pregnant cows which are predisposed to postpartum obstetric pathology, that occur in weak parturition activity, failure to release the placenta, uterus subinvolution, endometritis and postpartum hypocalcaemia, unfavorable prognostication is the low concentration in blood plasma of general calcium, inorganic phosphorus and carotene.


2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Imran Khan ◽  
Saleem Ur Rahman ◽  
Muhammad Ilyas ◽  
Ayaz Ali Khan

Vehicles refueling station workers are a class of labors prone to long term petroleum product toxicity due to their routine work at vehicles refueling stations. Health problems posed by the pollutants at the work environment of an individual are closely linked to the nature and level of exposure to these hazardous chemicals. The adverse effect of the toxicants in such environment is a common phenomenon in all the developing countries. The awareness about this problem is lacking in Pakistan, particularly in Khyber Pakhtunkhwa. In present study, blood biochemical parameters like calcium, cholesterol, glucose, magnesium, potassium, phosphorus and triglyceride levels were determined among workers and control individuals. The results of the workers showed an increase in glucose, cholesterol and phosphorus level; while significant decrease was observed in calcium and potassium level as compared to the control group of individuals. While the level of magnesium and triglyceride level was same in both groups. In comparative picture, the blood biochemical parameters were normal in control as that of workers. Further study may be conducted to investigate the effect of such environment on other blood parameters and large size population may be included in the study.


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