Effect of Chelated Mineral Mixture on Blood Biochemistry, Hormone and Mineral Status in Repeat Breeder Buffaloes in Tribal Areas of Dahod District in Gujarat, India

2019 ◽  
Vol 15 (01) ◽  
pp. 40-44 ◽  
Author(s):  
P M Joshi ◽  
D Patel ◽  
P D Patel

An on-farm trial for 90 days was conducted at four tribal villages to assess the effect of chelated mineral mixture (CMM) supplementation on blood biochemistry as well as hormonal and mineral status, nutrient intake and reproductive performance of the repeat breeder buffaloes (n = 24). The animals selected were randomly divided into two groups of 12 animals each. The group T1 was control group (farmer’s feeding schedule), and T2 treatment group (T1 + CMM @ 50 g/animal/day). Blood collection was done at 0, 45 and 90 days of the experiment from both the groups for assessment of blood biochemical, hormonal and mineral status of the animals. Average DM, DCP and TDN intake in repeat breeder buffaloes calculated as per information collected from farmers were statistically similar among both the groups and were as per ICAR requirements of animals. Results revealed significant (p less than 0.05) improvement in hemoglobin, serum glucose, total protein, total cholesterol, progesterone, and macro (Ca, P)–micro (Zn, Fe, Cu, Co, Mn) mineral status of chelated mineral supplemented group as compared to control animals. The number of days taken for a successful conception in repeat breeder buffaloes as well as the cost of feeding was also reduced upon supplementation of CMM in T2 as compared to T1 group. The control group recorded a 22.12% higher cost of rearing than the treatment group during the experimental period. The findings revealed the beneficial role of cheated minerals supplementation in improving health, nutritional and reproductive status of repeat breeding buffaloes.

2020 ◽  
Vol 15 (03) ◽  
pp. 61-65
Author(s):  
DC Patel ◽  
PM Joshi ◽  
PD Patel ◽  
NP Sarvaiya

An on-farm trial for 90 days was conducted at four tribal villages to assess the effect of chelated mineral mixture (CMM) supplementation on blood biochemistry as well as hormonal and mineral status, nutrient intake and reproductive performance of the anoestrus buffaloes (n=24). The animals selected were randomly divided into two groups of 12 animals each. The group T1 was the control group (farmer’s feeding schedule), and T2 treatment group (T1 + CMM @ 50 g/animal/day). Blood collection was done on days 0, 45, and 90 of the experiment from the animals of both the groups for assessment of blood biochemical, hormonal, and mineral status of the animals. Average DM, DCP, and TDN intake in anoestrus buffaloes calculated as per information collected from farmers were statistically similar among both the groups and were as per ICAR requirements of animals. Results revealed significant (p less than 0.05) improvement in hemoglobin, serum glucose, total protein, total cholesterol, progesterone, estradiol and macro (Ca, P) – micro (Zn, Fe, Cu, Co, Mn) mineral status of chelated mineral supplemented group as compared to control animals. The number of days taken to come into oestrus in anoestrus buffaloes (51.22±3.78 vs 75.00±6.18 days for 9 vs 5 animals), as well as the cost of feeding (Rs. 5441 vs 8039), was also significantly (pless than 0.05) reduced upon supplementation of CMM as compared to control group. The control group recorded a 32.32% higher cost of rearing than the treatment group during the experimental period. The findings revealed the beneficial role of chelated minerals supplementation in improving the health, nutritional and reproductive status of anoestrus buffaloes.


2020 ◽  
Vol 15 (03) ◽  
pp. 16-20
Author(s):  
AJ Dhami ◽  
KB Vala ◽  
FS Kavani ◽  
BB Bhanderi ◽  
SC Parmar

Forty advanced pregnant Jafarabadi buffaloes of 2-4 parity were divided into two equal groups, viz., control and treatment (n = 20 each). The animals of control group were maintained on routine farm feeding schedule and those under treatment group were subjected to additional oral supplements daily with 50 g of chelated mineral mixture and 150 g of bypass fat along with concentrates for 6 weeks prepartum till 2 weeks postpartum, and then bypass fat was given @ 15 g/litre of milk produced till 60 days postpartum. All the buffaloes were subjected to blood collection on day -45, -30, -7, 0, 7, 15, 30, 45 and 60 peripartum. Postpartum animals were followed at weekly interval by per rectal palpation and ultrasonographically for uterine involution, first postpartum estrus and conception. The overall mean blood glucose of prepartum period increased significantly (p less than0.01) on the day of calving and reduced back to prepartum levels within next 7 days postpartum in both the groups. The buffaloes supplemented with peripartum nutrients had significantly (p less than 0.01) higher blood glucose levels than the control group. The mean plasma total protein (7.96 ± 0.04 g/dl) and thyroid hormones T3 (1.44 ± 0.05 ng/ml) and T4 (28.25 ± 0.86 ng/ml) were found to be within the normal range, and did not vary between sampling days or between groups. The peripartum supplementation of bypass fat did not influence the levels of plasma total cholesterol. However, the levels of β-hydroxybutyrate (BHBA) and non-esterified fatty acids (NEFA) tended to be higher in control than treatment group at most of the peripartum intervals. The period of uterine involution (32.75 ± 0.57 vs 37.00 ± 0.56 days) and service period (107.10 ± 4.43 vs 133.65 ± 6.04 days) were significantly (p less than 0.05) shorter with higher postpartum fertility (85 vs 50% CR) in nutrient supplemented than control group. It was concluded that peripartum bypass fat and mineral supplementation prevented negative energy balance and/or ketosis and improved postpartum fertility in high yielding Jaffrabadi buffaloes.


2019 ◽  
Vol 15 (02) ◽  
pp. 14-17
Author(s):  
K K Hadiya ◽  
A J Dhami ◽  
D V Chaudhari ◽  
P M Lunagariya

This study was initiated on 24 prepubertal Holstein x Kankrej crossbred heifers of nearly identical age (7-9 months) and body weight (130-140 kg) at University farm to evaluate the effect of high plane of nutrition on blood biochemical and minerals profile and the age at puberty. Twelve heifers were managed under routine farm feeding (control) and the rest 12 under ideal optimum feeding regime (treatment) that included extra 1 kg concentrate, 30 g min mix and ad-lib dry fodder. The body weight and ovarian ultrasonography together with blood sampling was carried out at monthly interval from 10 to 18 months of age to study the ovarian dynamics and blood biochemical changes. High plane of nutrition to growing heifers was beneficial in reducing the age of onset of puberty (by 2-3 months) compared to routine farm fed group. The mean plasma total protein and cholesterol concentrations showed a rising trend with significant variations from 10 to 16 months of age, where it got mostly stabilized indicating adult profile. The activity of enzymes GOT and GPT also rose gradually and significantly from 10 months till 14-15 months of age, and thereafter it remained more or less static till 18 months of age. The levels of both these enzymes were higher, with lower protein and cholesterol, in control than the treatment group from 15-16 months of age onwards. The mean plasma levels of both calcium and phosphorus increased gradually and significantly with advancing age till 16-17 months of age, with little higher values in supplemented than a control group. The plasma levels of zinc, iron, copper, and cobalt also showed rising trend with significant differences between 10th and 12th-14th months of age, and from 15th to 18th months of age the levels were statistically the same in all the groups with slightly higher values in the treatment group.


2016 ◽  
Vol 2 (2) ◽  
pp. 274-278
Author(s):  
Masum Billah ◽  
Md Humayun Kabir ◽  
Md Hafizur Rahman ◽  
Md Abdul Hamid

The present study was carried out to assess the effect of concentrate on growth performances of both male and female lamb. For this purpose twenty four (12 male and 12 female) lamb aged about 5-6 months were selected and divided into three groups (Treatment group T1, T2 and control group T0). In each group having 4 males and 4 females lamb, all lambs were supplied green roughages ad-libitum, control group T0 was allowed no concentrate and treatment group T1 & T2 were supplied 100 gm & 200 gm concentrate mixture respectively for 90 days experimental period. Animals were weighed at 15 days interval. Significant differences of live weight gain among these three groups were found. Allowing 100 gm (T1) concentrate mixture along with green grass improved growth rate in both male and female lamb. Increasing of concentrate supplementation improved live weight gain (found in T2 group). Total live weight gain (kg) and average daily live weight gain (g/d) were 4.25±0.52 kg and 47.20±5.75 g, 5.38±0.83 kg and 59.73+9.17 g and 7.00±0.54 kg and 77.78±6.00 g in male lambs and 2.25±0.21 kg and 25.00±2.34 g, 3.18±0.32 kg and 35.39±3.63 g and 3.81±0.24 kg and 42.33±2.77 g in female lambs for the To, T1 and T2 groups respectively. Live weight was significantly (P<0.05) higher in male than female lambs. The results showed that 100 g concentrate supplementation with green roughages improved growth rate in lambs (male and female) under stall feeding condition. Increase of supplementation might improve nutrients supply to promote higher live weight gain.Asian J. Med. Biol. Res. June 2016, 2(2): 274-278


2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Ji Hyun Lee ◽  
Mary Gulumian ◽  
Elaine M. Faustman ◽  
Tomomi Workman ◽  
KiSoo Jeon ◽  
...  

Background. To investigate the effect of subacute intravenous administration AgNP (silver nanoparticles, 10 nm) and AuNP (gold nanoparticles, 12.8 nm) and AgNP/AuNP mixture to blood biochemistry, hematology, and platelet coagulation, subacute toxicity study was conducted. Methods. AuNP and AgNP in which their size distribution was not statistically different, mixed or separate, were injected into the caudal vein of male Sprague-Dawley rats for 4 weeks. The rats were allowed to recover for a further 4 weeks in order to examine systemic toxicity expressed in the blood biochemistry and hematology. The dose groups (5 males per group for the administration and 3 males for the recovery) consisted of 7 divisions, i.e., control, AgNP (with a low dose of 10 μg/kg/day and a high dose of 100 μg/kg/day), AuNP (with a low dose of 10 μg/kg/day and a high dose of 100 μg/kg/day), and mixed AgNP/AuNP (with a low dose of 10/10 μg/kg/day and a high dose of 100/100 μg/kg/day). Results. There were no significant dose-related changes in the hematology and blood biochemical values for the rats. Coagulation time in terms of the active partial thromboplastin time (APTT) and prothrombin time (PT) did not show any significant changes, when compared to the control group. Conclusion. The subacute injection of AuNP and AgNP or their mixture did not induce any noticeable systemic toxicity.


Author(s):  
Sandhya S. Chaudhary ◽  
Tanvi D. Manat ◽  
Virendra Kumar Singh ◽  
Sanjay B. Patel

The present study was undertaken to study levels of vitamin A and β-carotene in blood of postpartum Surti goats. 40 Surti goats were selected comprising of 20 goats with recent parturition (treatment group) and 20 non-pregnant animals (control group). Blood collection was done on 0, 7, 14, 21, 30 and 45 day post-kidding (treatment group) and once from control group and analyzed for Vitamin A and β-carotene. Both the analytes were significantly low on 0 day. Significant difference (P Less Than 0.05) in vitamin A as well as β-carotene (P Less Than 0.01) was observed between 0 and 21st day postpartum followed by non-significant difference in vitamin A after 21 days. However, β-carotene values at 21st day significantly differed from those at 30th and 45th day. Non–significant difference between control and treatment group from 7th day onwards was observed in vitamin A. β-carotene values of treatment group were significantly lower at 0 and 21st day postpartum as compared to control. It was concluded that on the day of kidding there is decrease in circulatory levels of vitamin A and β-carotene and this decrease can be used as an indicator of stress.


Author(s):  
Renata Franco dos SANTOS ◽  
Shamila Évellem Magalhães da SILVA ◽  
Monique Ferreira BRANDÃO ◽  
Maria Vitória Marialva da Silva LÔBO ◽  
Deborah Jacob Freire da PAZ ◽  
...  

ABSTRACT This study aimed evaluate energetic mobilization in tambaqui submitted to fasting, after a one-day refeeding. 120 tambaqui juveniles were distributed in 12 310L polyethylene boxes. Three treatments were evaluated: Control (14 days of feeding); Fasting for 14 days; and Refeeding (13 days fasting and one day of feedback). After the experimental period, the fish were anesthetized with eugenol for blood collection and serum and plasma were used to measure glucose, triglycerides, cholesterol and serum protein. Subsequently, fish were euthanized to remove liver and mesenteric fat and were used to determine hepatic glycogen and lipid and mesenteric fat index. The results were submitted to ANOVA and the means compared by Tukey test when statistical significance was observed (P <0.05). Glucose, triglycerides and serum protein decreased after fasting, differing statistically with the control. Refeeding resulted in the recovery of three blood indicators. Liver analysis shows glycogen was consumed intensely during fasting and partially recovered after refeeding, when compared to control group. The results obtained in this study suggest that the 14-day fast was not harmful to the fish and the tambaqui are able to quickly adjust their metabolism according to their nutritional status.


2021 ◽  
Vol 34 (1) ◽  
pp. 154-160
Author(s):  
Joselaine Bortolanza Padilha-Boaretto ◽  
Priscila Michelin Groff-Urayama ◽  
Suelen Maria Einsfeld ◽  
Cleverson de Souza ◽  
Angélica Signor Mendes ◽  
...  

Objective: The effect of environmental enrichment on the behaviour of piglets in the nursery phase was evaluated.Methods: A total of 450 hybrid pigs (21 day old), including both females and uncastrated males, weighing approximately 6 kg, were distributed in a completely randomised design with 3 treatments and 3 replicates of 50 animals each. The treatments were: i) pen without environmental enrichment (control), ii) treatment consisting of continuous environmental enrichment (CEE) with rubber balls throughout the experimental period, and iii) treatment consisting of environmental enrichment with washed balls (EEWB) during the whole experimental period which were removed daily for washing. For the behavioural evaluation, 10 animals were randomly selected per replicate. The behavioural assessments were performed once a week, from 8 am to 6 pm, using images captured with a video camera. The data were submitted to non-parametric analyses, the means were compared using the Bonferroni test, and Person’s correlations were also calculated.Results: A statistical difference (p = 0.001) was observed in the B5 (playful) behaviour; the animals in the EEWB treatment group had a higher frequency of this behaviour than animals in the control treatment group. The animals in the control group showed a higher frequency of B7 behaviour (lying down) (p = 0.026) than those in the EEWB and CEE treatment groups. The animals in the control group had a higher frequency of the B9 (belly nosing) behaviour than those in the EEWB group (p = 0.015). There was a tendency towards a higher frequency of behaviour B3 (walking in the pen) (p = 0.067) when the animals received the control treatment than treatments EEWB or CEE.Conclusion: The use of an enriching object improved the animal welfare and altered the correlation between the evaluated behaviours compared to the animals that did not have environmental enrichment.


Author(s):  
Imtiyaz Qayoom ◽  
Masood H. Balkhi ◽  
Feroz A. Shah ◽  
Bilal A. Bhat

Present study was aimed to investigate the effects of two organophosphate pesticides (dimethoate and chlorpyrifos) on some blood biochemical parameters of juveniles common carps (Cyprinus carpio var. communis). Fingerlings were subjected to bioassays divided into 5 experimental groups in range finding test on the basis of which the doses for definitive tests were selected. The acute bioassay toxicity tests were carried out in triplicates for 96 hours with a control group run parallel to the experiment. Data obtained was analyzed as per Finney’s probit to determine LC50 values. The mean lethal concentration (LC50) values for dimethoate and chlorpyrifos were found 1.1ppm and 3.8ppb respectively. The samples were investigated for various blood biochemical indices such as total protein (TP), serum albumin (SA), serum globulin (SGLO), alanine aminotransferase (ALT), asphartate aminotransferase (AAT), serum bilirubin (SB), serum glucose (SGLU), serum cholesterol (SC), and lactate dehydrogenase (LDH). All the liver enzymes (AAT, ALT, SB and LDH) and glucose (SGLU) were found increased while as TP, SA, SGLO, SB and SC were found decreased than the normal. Chlorpyrifos, was however, found to alter the biochemical indices more profoundly than dimethoate. The results indicated the organophosphate pesticides are intensely harmful for fish health. Their entry to natural waterbodies must be stopped besides ensuring their judicious application around the catchment area.


2019 ◽  
Vol 15 (01) ◽  
pp. 45-49
Author(s):  
K B Vala1 ◽  
A J Dhami ◽  
F S Kavani ◽  
V P Ramani ◽  
S C Parmar

This investigation was carried out on 40 advanced pregnant Jafarabadi buffaloes of 2–4 parity equally divided into two groups, viz., control and treatment groups. The control animals were maintained on standard routine farm feeding, and the animals of the treatment group received additional oral supplements of 50 g chelated mineral mixture and 150 g bypass fat daily along with concentrates for 6 weeks prepartum and 8 weeks postpartum. The bypass fat was given @ 15 g/liter of milk produced limiting up to 200 g/head/day from 15–60 days postpartum. Ten animals in each control and treatment group further received Inj. Stimvet 5 mL (micro-minerals) around day 45 prepartum and on day of calving. Half of these Stimvet treated and control subgroups also received ecbolic Exapar (n = 5) 2 boli/day for 4 consecutive days postpartum. Blood samples were obtained from all animals by jugular vein puncture on the day –45, –30, –7 and 0 (day of calving), 7, 15, 30, 45, 60 peripartum for estimation of plasma trace minerals, viz., zinc, iron, copper, cobalt, and manganese. The plasma zinc and copper concentrations were found to be higher on day 30 prepartum and again on day 7 postpartum in Stimvet injected groups.Moreover, the overall pooled mean concentration of zinc was significantly higher in nutrients supplemented than the control group. The plasma concentrations of iron, cobalt, and manganese did not reveal any specific trend or significant variations between groups or between periods within any of the groups. Further, the peripartum oral nutrients supplementation significantly shortened the periods of placental expulsion time and uterine involution, with early onset of postpartum first estrus, apparently shorter service period and enhanced pregnancy rate in comparison to control group. The influence of Stimvet and Exapar alone or in combination, however, did not show a significant beneficial effect on these traits, except shortened placental expulsion time and higher plasma zinc and cobalt status. Hence, the Jafarabadi buffalo keepers may be advised to provide additional oral nutrients supplementation in the form of bypass fat and chelated minerals over routine feeding to their animals during a transitional period for improved postpartum fertility.


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