scholarly journals Effect of Reduced Dietary Crude Protein with Balanced Limiting Amino Acids on Egg Weight and Reproductive Performance of Swarnadhara Layers

Author(s):  
Beena C. Joseph ◽  
Jayanaik . ◽  
C. S. Nagaraja

A study was conducted in Swarnadhara (SD) layers to find out the effect of reducing crude protein (CP) with supplementation of limiting amino acids to compensate the deficiency of CP. Egg weight and reproductive parameters such as fertility, hatchability, number of saleable chicks and day one old chick weight were studied in 240 SD layers in a completely randomized design in 5 treatments with 6 replications of 8 birds in each. The control diet (T1) was based on corn soy bean meal formulated with 16% CP and 2700 Kcal ME following the ICAR (2013) specifications recommended for improved native chicken breeds. The diets T2 to T5 were made isocaloric to control and composed with 15.5, 15.0, 14.5 and 14.0% CP, respectively. The levels of limiting amino acids namely methionine, lysine, threonine and tryptophan were met as that of control by adding synthetic preparation wherever necessary. Egg weight showed similar effects in all treatments at 32, 36 and 40 weeks of age. Reproductive parameters were recorded in four consecutive hatches and were statistically analyzed. Results showed similar performances in T1, T2, T3 and T5, but were different in T4 in terms of fertility, hatchability and number of saleable chicks. There was no significant difference in progeny chick weight among different treatment groups indicating amino acid supplementation supported equal performance in low CP diets in all the reproductive parameters.

2023 ◽  
Vol 83 ◽  
Author(s):  
A. Ayub ◽  
F. Rasool ◽  
N. Khan ◽  
S. N. Qaisrani ◽  
S. Parveen ◽  
...  

Abstract Ninety days study was conducted in hapas installed in earthen ponds. Fish of an average initial weight (220g) were evenly distributed in triplicate groups within fifteen hapas. Five experimental diets labeled as T1 (25% CP and NRC recommended amino acid level) as control diet, T2 (with 2% low protein and 5% amino acid supplementation), T3 (with 2% low protein and 10% amino acid supplementation), T4 (with 4% low protein and 10% amino acid supplementation) and T5 (with 4% low protein and 20% amino acid supplementation) were prepared. Fish were fed with @3% of their body weight twice a day at 10.00 & 16:00 hour. Significantly higher percent weight gain (420.18 ± 66.84a) and specific growth rate (13499.33±1273.54a) along with improved feed conversion ratio (1.29 ± 0.09b) and hundred percent survivals were recorded during the trial. Furthermore proximate analysis of meat showed significant improvement in the crude protein level (81.77 ± 0.19a) served with diet containing 20% limiting amino acids mixture. Therefore, limiting amino acids can be a source of cost effective feed and use safely in L. rohita diet.


2021 ◽  
Vol 47 ◽  
pp. e599
Author(s):  
Rudã Fernandes Brandão SANTOS SANTOS ◽  
Mayara Schueroff SIQUEIRA ◽  
Ryuller Gama Abreu REIS ◽  
Weliton Vilhalba SILVA ◽  
Henrique Momo ZIEMNICZAK ◽  
...  

Symphysodon aequifasciatus is a fish with a disk-shaped body and bright colors, important characteristics of ornamental fish. We evaluated amino acid supplementation strategies to reduce crude protein in the diet for evaluation of performance, the content of digestive enzymes, liver metabolism, and intestinal histopathology. A total of 180 fish were randomly distributed in 12 separate 50 L glass aquariums, consisting of a completely randomized design with four treatments (DC - Control diet with 34.4% crude protein; DL - Control diet plus 1% of lysine; DEAA - Control diet plus 1% free essential amino acids (threonine, phenylalanine, leucine, valine, arginine, and tryptophan); and DHP - Diet with a high level of crude protein 48.4%), three repetitions, lasting 60 days. The use of DL and DEAA diets resulted in higher intestinal villus height and higher zootechnical performance. The use of DL diet increased alkaline phosphatase and digestive amylase activity. The use of DHP diets promotes severe liver changes due to increased activity of Alanine aminotraserase. Therefore, it was possible to observe that the use of amino acids can supply the nutritional need of blue discus. Supplementation of diets with AAs allows the reduction of dietary protein, which is a strategy for feeding management.


Author(s):  
Beena C. Joseph ◽  
Jayanaik T. Munegowda ◽  
Chandrapal . ◽  
K. Singh ◽  
V. Malathi

A six-week trial was conducted to determine the effect of lowering dietary crude protein levels with supplementation of limiting amino acid on growth performance, and carcass characteristics in one of the Indian improved crossbred Swarnadhara chicks in a completely randomized design having five treatments and four replications of 20 birds in each. Five isocaloric (2800 ME Kcal//kg) experimental diets based on corn-soy bean meal were formulated in a gradual crude protein decline from 21 % (control T1) to 19% by 0.5% interval. The levels of limiting amino acids namely methionine, lysine, threonine and tryptophan were met as that of control (T1) by adding synthetic preparations. Highest body weight was recorded in 2% crude protein reduced group (T5) which was comparable with control (T1). However, cumulative feed intake and feed conversion ratio of different treatments were not significantly different. Moreover, carcass parameters like dressing percentage, breast meat yield, liver and heart weights remained unaffected by the dietary treatments. Relative gizzard weight and abdominal fat weights showed variations and lower dietary protein diets were comparable with control diet (T1). In summary, crude protein content of Swarnadhara chick diet can be reduced to 19% with essential AA fortification without any adverse effect on growth performance and carcass characteristics during 0-6 weeks of age.


2011 ◽  
Vol 35 (3) ◽  
pp. 591-597 ◽  
Author(s):  
Elisangela Minati Gomide ◽  
Paulo Borges Rodrigues ◽  
Márcio Gilberto Zangeronimo ◽  
Antônio Gilberto Bertechini ◽  
Luziane Moreira dos Santos ◽  
...  

The objective of this study was to evaluate the effect of reduced-crude protein (CP) and available phosphorus (aP) diets, added with phytase and amino acids for broilers in the growing (14 to 21 days old) and retirement (35 to 42 days) phases on nitrogen, potassium and phosphorus balance. Ninety Cobb broilers were distributed in a completely randomized design with three treatments and six replications with five and three birds per experimental unit in the growing and retirement phases, respectively. Diets were elaborated to contain reduced CP and aP levels (19 and 17% CP in the growing phase, with 0.34% aP; and 18 and 16% CP in the retirement phase, with 0.30% aP), supplemented with crystalline amino acids and 500 FTU of phytase/kg diet. These were compared to a control diet (21% CP and 0.46% aP for growing phase and 19% CP and 0.40% aP for retirement phase). The reduction of CP in four percentual units reduced the N excretion and increased the retention of this element, showing that the phytase had no influence on N balance when used the crystalline amino acids in the diets. The phytase reduced the P excretion and increased the retention ones. The potassium excretion decreased in reduced-CP diets, but this excessive reduction decreased the retention of this element, except in the retirement phase. It was concluded that the reduced-CP and aP diets, supplemented with phytase and amino acids, are efficient in reducing the pollutant power of the broilers excrements in the growing and retirement phases of production.


2021 ◽  
Vol 34 (2) ◽  
pp. 196-202
Author(s):  
L.A. F. Akinola ◽  
B. M. Oruwari

he response of Isa Brown laying hens during 77–85 weeks of age to peeled unfermented cassava root meal (CRM) as total substitute for maize in diet was examined. Eighty (80) hens were assigned to four dietary treatment groups (T1,T2,T3 and T4) in a completely randomized design. The control diet (CD) contained 40% maize while the proportion of maize in the CD was replaced with 100, 110 and 120% CRM amounting to 40, 44 and 48% of cassava in diet for T2, T3, and T4 respectively. All diets were isocaloric and isonitrogenous. The CRM diets produced statistically significant (p<0.05) improvements by leading to lower feed intake, better feed conversion and increased hen day production (HDP) with increasing levels of CRM in diet. Although the eggs obtained from birds fed the CD had significantly higher yolk weight than those from the CRM, there was no significant difference (p>0.05) in egg weight in all treatment groups. The best overall performance was achieved from birds fed 48% (120% replacement) cassava diet.


2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Izabel Aparecida Soares ◽  
Mauro Sérgio Téo ◽  
Carlise DEBASTIANI ◽  
Suzymeire BARONI ◽  
Vanessa Silva RETUCI

O trabalho teve por objetivo verificar diferenças entre rendimento do concentrado proteico e proteína bruta da folha da mandioca (Manihot esculenta Crantz), obtidos a partir de três variedades comerciais: branca, cascuda e vermelha. As manivas foram plantadas seguindo o delineamento experimental inteiramente casualizado com três repetições. Nas comparações entre as variedades, considerou coletas escalonadas pós-plantio, realizadas aos 12, 14 e 16 meses. O concentrado proteico foi obtido a partir da farinha das folhas inteiras e submetido ao método de termo - coagulação ácido e a proteína bruta pelo método padrão AOAC. Os dados foram submetidos a análise de variância e comparados pelo teste de Tukey - 5% de probabilidade. Os resultados não indicaram diferença significativa entre as médias obtidas para rendimento de concentrado proteico. Para a variável porcentagem de proteína bruta a variedade Branca foi a que apresentou maior valor, com média de 46,25%, seguida pela Cascuda e Vermelha, 44,52% e 37,30%, sucessivamente. Conclui-se que outros estudos devem avaliar condições que possam influenciar no teor de proteína foliar, como clima e solo, e, avaliar os níveis de ácido cianídrico de cada variedade, indicando qual das três é a melhor para a extração do concentrado proteico das folhas. Palavras chaves: Manihot esculenta Crantz, variedades comerciais, concentrado proteico, proteína bruta. ABSTRACT: The study aimed to assess the differences between income protein concentrate, crude protein of cassava leaf (Manihot esculenta Crantz), obtained from three commercial varieties: white, red and cascuda. The cuttings were planted following the completely randomized design with three replications. Comparisons between the varieties considered after planting staggered collections, held on 12, 14 and 16 months. The protein concentrate was obtained from flour of whole sheets and subjected to the term method - acid coagulation and crude protein by AOAC standard method. The data were submitted to ANOVA and Tukey test - 5% probability. The results indicated no significant difference between the mean values obtained for protein concentrate income. For the variable percentage of crude protein White variety showed the highest, with an average of 46.25%, followed by cascuda and Red, 44.52% and 37.30%, successively. We conclude that further studies should evaluate conditions that may affect the leaf protein content, such as climate and soil, and evaluate the hydrocyanic acid levels of each variety, indicating which of the three is the best for the extraction of protein concentrate from leaves Key words: Manihot esculenta Crantz, commercial varieties, protein concentrate, crude protein.


2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hamid Raieszadeh ◽  
Vahid Noaman ◽  
Mehrdad Yadegari

270 Ross broiler chickens of twenty days old were housed in 18-floor pens in a completely randomized design with six treatment groups and three replicate groups and fifteen chicks per each pen. The treatment groups (1–6) consisted of 0, 10, 20, 30, 50, and 70 ppm of nanocide in drinking water, respectively. At 26 days of age, 3 chickens were selected randomly for echocardiography using a 7.5 MHz linear probe, and the left ventricular internal diameter at the end of diastole (LVIDd), left ventricular internal diameter at the end of systole (LVIDs), left ventricular fractional shortening (LVFS), ejection fraction (EF), stroke volume (SV), interventricular septum thickness at the end of systole (IVSTs), and interventricular septum thickness at the end of diastole (IVSTd) were evaluated. LVIDd and LVIDs in group six were of higher rate than other groups and showed statistically significant differences with groups two, three, and four (P<0.05). LVFS, percentage of EF, and IVSTd were minimum in group six and had significant difference with other groups (P<0.05). The results of this study showed that prescription of high dosage of nanocide leads to cardiovascular problems with decrease in myocardial contractility and increase in the internal diameter of left ventricle.


Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 520 ◽  
Author(s):  
Anne Daly ◽  
Sharon Evans ◽  
Satnam Chahal ◽  
Saikat Santra ◽  
Alex Pinto ◽  
...  

Introduction: In phenylketonuria (PKU), evidence suggests that casein glycomacropeptide supplemented with rate-limiting amino acids (CGMP-AA) is associated with better protein utilisation and less blood phenylalanine (Phe) variability. Aim: To study the impact of CGMP-AA on blood Phe variability using 3 different dietary regimens in children with PKU. Methods: This was a 6-week randomised controlled cross-over study comparing CGMP-AA vs. Phe-free l-amino acids (l-AA) assessing blood Phe and tyrosine (Tyr) variability over 24 h in 19 children (7 boys) with PKU, with a median age of 10 years (6–16). Subjects were randomised to 3 dietary regimens: (1) R1, CGMP-AA and usual dietary Phe (CGMP + Phe); (2) R2, CGMP-AA − Phe content of CGMP-AA from usual diet (CGMP − Phe); and (3) R3, l-AA and usual dietary Phe. Each regimen was administered for 14 days. Over the last 48 h on days 13 and 14, blood spots were collected every 4 h at 08 h, 12 h, 16 h, 20 h, 24 h, and 04 h. Isocaloric intake and the same meal plan and protein substitute dosage at standardised times were maintained when blood spots were collected. Results: Eighteen children completed the study. Median Phe concentrations over 24 h for each group were (range) R1, 290 (30–580), R2, 220 (10–670), R3, 165 (10–640) μmol/L. R1 vs. R2 and R1 vs. R3 p < 0.0001; R2 vs. R3 p = 0.0009. There was a significant difference in median Phe at each time point between R1 vs. R2, p = 0.0027 and R1 vs. R3, p < 0.0001, but not between any time points for R2 vs. R3. Tyr was significantly higher in both R1 and R2 [70 (20–240 μmol/L] compared to R3 [60 (10–200) μmol/L]. In children < 12 years, blood Phe remained in the target range (120–360 μmol/L), over 24 h, for 75% of the time in R1, 72% in R2 and 64% in R3; for children aged ≥ 12 years, blood Phe was in target range (120–600 μmol/L) in R1 and R2 for 100% of the time, but 64% in R3. Conclusions: The residual Phe in CGMP-AA increased blood Phe concentration in children. CGMP-AA appears to give less blood Phe variability compared to l-AA, but this effect may be masked by the increased blood Phe concentrations associated with its Phe contribution. Reducing dietary Phe intake to compensate for CGMP-AA Phe content may help.


1990 ◽  
Vol 38 (3B) ◽  
pp. 609-622
Author(s):  
N.P. Lenis ◽  
J.T.M. van Diepen

Individual and group housed crossbred pigs 45 to 105 kg and 65 to 95 kg in experiments 1 and 2, respectively, were given basal diets with L-threonine 0.6, 1.2 and 1.8 g/kg. Positive and negative control diets contained total threonine 5.7 and 4.5 g/kg, respectively. To prevent other amino acids being limiting, the negative control diet was supplemented with lysine, methionine, tryptophan, isoleucine, histidine and valine. The positive control diet was supplemented with lysine and methionine. The requirement for total threonine of growing-finishing pigs for maximum growth performance was about 5.6 g/kg in a diet containing net energy 9.4 MJ/kg. This figure corresponds with about 4.7 g/kg apparent faecal digestible threonine and 4.3 apparent ileal digestible threonine. There was no difference between the growing and the finishing pigs. The requirement for ileal digestible threonine, relative to ileal digestible lysine requirement, was about 64%. It is concluded that dietary protein can be reduced by 2 percentage units without any adverse effect on growth performance, if limiting amino acids are sufficiently supplemented. (Abstract retrieved from CAB Abstracts by CABI’s permission)


2021 ◽  
Vol 10 (12) ◽  
pp. e339101220447
Author(s):  
Thiago de Sousa Melo ◽  
José Humberto Vilar da Silva ◽  
José Jordão Filho ◽  
Fernando Guilherme Perazzo Costa ◽  
Patrícia Emília Naves Givisiez ◽  
...  

The objective of this study is to evaluate the influence of five feed restriction programs (FRP) on broiler performance and carcass yield. 425 Cobb 500® broilers were distributed in a completely randomized design with five FRPs and five replicates with 17 broilers. The FRPs were: Program 1 (P1): consumption ad libitum (AL) of control diet from 14 to 42 days; P2: quantitative restriction (QTR) of 10% of AL consumption from 14 to 28 days and AL consumption from 29 to 42 days; P3: AL consumption from 14 to 28 days and QTR from 29 to 42 days; P4: qualitative restriction (QLR) of 10% of the level of crude protein and essential amino acids from 14 to 28 days and AL consumption from 29 to 42 days; and P5: AL consumption from 14 to 28 days and QLR from 29 to 42 days. The broilers of the AL treatment gained more weight (p≤0.01), but had a similar FCR (P>0.05) compared to broilers submitted to QTR from 29 to 42 days. In addition, broilers fed QTR from 14 to 28 days presented a lower FI and a better FCR (p≤0.01) in relation to broilers fed QLR of 14 to 28 (P4) and 29 to 42 days (P5). The broilers fed QTR of 14 to 28 days diet presented a similar FCR as broilers fed AL. The 10% reduction in AL consumption of 14 to 28 d is a viable economical alternative to feed broilers up to 42 days of age.


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