scholarly journals Improvement of Fertility in Postpartum Anooestrus Buffaloes by using Different Hormonal Protocols during Non Breeding Season

Author(s):  
A. D. Patil ◽  
S. K. Sahatpure ◽  
P. T. Jadhao ◽  
J. P. Korde ◽  
M. S. Patil ◽  
...  

The study was conducted on 40 postpartum anooestrus buffaloes randomly divided into four equal groups during non-breeding season. The buffaloes were selected based on ovarian inactivity, good body condition score and overall good health. Group I was administered Ovsynch protocol + Progesterone impregnated intravaginal device (PRID). Group II was administered PRID which was removed on 7th day and GnRH 10 μg i/m was given on day of AI. Group III was administered Ovsynch protocol and Group IV was kept as untreated control. 100, 80, 80 and 00 % animals responded in groups I, II, III and IV respectively. Fix timed AI was performed in all responded buffaloes. Calcium, phosphorous, cholesterol and protein level increased (p>0.05) in respect of pregnant animal than the non-pregnant animals, the rise was more in Group I than the other Groups.The conception rate was found to be 70.00, 50.00, 62.50 and 00.00 % in Group I, II, III and IVrespectively. The overall conception rate was observed to be significantly higher in Group I, i.e., Ovsynch + PRID followed by Group III, i.e. Ovsynch protocol alone in postpartum anooestrus buffaloes during nonbreeding season. It can be concluded that ovsynch with progesterone based protocol is very effective for oestrus induction and improved conception rate in postpartum anooestrus buffaloes during non breeding season.

2021 ◽  
Vol 22 (2) ◽  
pp. 292-297
Author(s):  
Anwar Rosyidi ◽  
Adji Santoso Dradjat ◽  
Lalu Ahmad Zainuri ◽  
Lalu Wirapribadi ◽  
Tjok. Suwendra Binetra ◽  
...  

The aim of this study was to evaluate odds ratio (OR) of management factors on infertility of artificial insemination results. Three traditional farmer groups were purposively choosed, based on percentage of calf born of AI program. Group I, with calves born of 61.22%, Group II with calves born of 38,89 %, and group III with calves born of 30,0%. The management factors evaluated were 1. ownerships of cattle, 2. raise cattle in separate pen with other, 3. cattle raised in pen dirty pen, 4. not enough feed given 5. body condition score. The data was obtained by dichotomy questioner and observation then evaluated using odds ratio (OR), when OR more than 1 indicated the high risk of factor to infertility. The results of OR in Group I showed that ownership (1,18), separated pen (0,54), dirty pen (2,11), less feed (3,20) and body condition (0,22). The results in Group II showed that ownership (0,83) , separated pen (1,12), dirty pen (0,14), less feed (2,08) and body condition (2,00). The results of odds ratios in Group III showed that ownership (1,63), separated pen (3,83), dirty pen (0,88), less feed (1,00) and body condition (0,12). These data indicated that ownerships, dirty pen, less feed and body condition were factors which may confounding with other factors. However less OR of separate pen were, in Group I (0,54), Group II (1,12) and Group III (3,83) consistently with number of calves produced. It can be concluded that separate pen was a factor which influence calves produced in using artificial insemination.


2016 ◽  
Vol 31 (2) ◽  
pp. 60-69
Author(s):  
SK Bhowmik ◽  
MGS Alam ◽  
M Shamsuddin ◽  
M Khatun

The effect of GnRH analogue (gonadorelin) was studied on the fertility of 35 postpartum anoestrus zebu cows and their crosses (Friesian, Sahiwal, Sindhi). Age (4-15 years), body weight (125-375 kg), body condition score (1.5-3; 1-5 scale), parity (1-9), postpartum intervals (4-12 months) of the cows were recorded. Oestrus was synchronized with two injections of PGF2-? analogue (Luprostinol, 15 mg) intramuscularly (i.m.) 10 days apart. The cows were divided into Group I (n = 11), Group II (n = 12) and Group III (n = 12). In Group I, 8 cows with oestrus were inseminated without gonadorelin treatment. In group II, 6 cows in oestrus were treated with gonadorelin (0.25 mg) i.m. just prior to insemination. In group III, 3 days after the second injection of luprostinol, cows with smooth ovaries were treated with gonadorelin followed by luprostinol injection 9 days later, which resulted in oestrus in 8 of the 12 cows, which were inseminated. Cows without oestrus signs were inseminated at fixed-time (72 and 96 hours after the last luprostinol injection). In group I, II and III, 5, 6 and 8 cows became pregnant, respectively (P>0.05). More cows became pregnant at observed oestrus (68.2%) than at fixed-time insemination (30.8%; P<0.05). Insemination in observed oestrus is more effective than at fixed-time. GnRH analogue may have favourable effect on the induction of oestrus by PGF2-? analogue.Bangl. vet. 2014. Vol. 31, No. 2, 60-69


Author(s):  
J. P. Lakher ◽  
M. K. Awasthi ◽  
J. R. Khan ◽  
M. R. Poyam

The present study was conducted to investigate the efficacy of Ovsynch and Ovsynch plus protocol in postpartum (day 60) Sahiwal cows (n=18). Animals were randomly divided into three equal groups, viz., Ovsynch group Ovsynch plus group and Control group. Animals of group I (n = 6) were treated with traditional Ovsynch protocol. The animals (n = 6) of group II were treated with Ovsynch plus protocol which consisted of an initial intramuscular injection of eCG (Folligon) @ 250 IU on day 60 postpartum followed 3 days later by GPG (Ovsynch) protocol. In group-III Control, no treatment was given to animals (n = 6). Treated animals were inseminated at a fixed time between 14 and 20 hrs after second GnRH injection, irrespective of estrus detection. Blood samples were collected from each animal on days 50 and 60 postpartum to determine the status of cyclicity in animals based on serum concentrations of progesterone (P4). A third blood sample was collected on the day of prostaglandin treatment to determine the response of first GnRH injection. Four animals each were cyclic, and two were acyclic in both treatment groups. Four animals each responded to first GnRH treatment in both treatment groups. Similarly, two animals each got conceived giving conception rate of 50% (2/4) in each treatment. In the control group, one out of 6 animals got conceived yielding 16.66 % conception rate (1/6) during the study period. It may be thus concluded that Ovsynch and Ovsynch plus protocol may be used during the early postpartum period to improve the reproductive efficiency in postpartum Sahiwal cows.


Author(s):  
Parker A Henley ◽  
Frank A Ireland ◽  
Igor F Canisso ◽  
J Lannett Edwards ◽  
Daniel W Shike

Abstract This study evaluated the effect of heifer development system on body weight (BW), body condition score (BCS), fescue toxicosis symptoms, reproductive performance, and subsequent calf growth of fall-calving beef heifers. Angus × Simmental heifers [n = 399; 240 ± 20.0 kg initial BW; age = 252 ± 20 d] were stratified by BW and BCS and assigned to 1 of 12 groups in each of the 2 production years. The study utilized a stratified randomized design. Pens were randomly assigned to 4 treatments: drylot (DL) development (fed ad-libitum diet consisting of 90% hay and 10% DDGS on a dry matter basis), grazing endophyte-infected fescue supplemented daily (2.3 kg as-fed/heifer/d; 50:50 mix of soybean hulls and DDGS; E+/S), grazing endophyte-infected fescue and supplemented from the midpoint of treatment period until breeding (4.5 kg as-fed/heifer/d; 50:50 mix of soybean hulls and DDGS; E+/LS), and grazing novel endophyte-infected fescue with no supplement (NE+/NS). Treatments ceased on d 168 [time of artificial insemination (AI)] and heifers were commingled and managed as a group through second breeding season. Heifers in DL had greatest (P ≤ 0.05) BW and BCS from d 28 until d 254. Furthermore, E+/S heifers had greater (P ≤ 0.05) BW and BCS than both E+/LS and NE+/NS from d 28 until d 168. On d 56 and 84, E+/LS heifers had lower (P ≤ 0.05) BW and BCS compared to NE+/NS, but on d 148 treatments reranked and E+/LS remained at a greater (P ≤ 0.05) BW and BCS compared to NE+/NS through the first breeding season. Drylot heifers had greatest (P ≤ 0.05) percentage cycling and percentage of mature BW at AI (66.6%) and had greater (P ≤ 0.05) AI and overall pregnancy rates compared to E+/LS and NE+/NS. The E+/S (55%) and E+/LS (53.7%) heifers were developed to a greater (P &lt; 0.01) percentage of mature BW than NE+/NS (49.3%). A greater (P ≤ 0.02) percentage of DL and E+/S heifers were pregnant at the end of the first breeding season (89.3 and 85.1%; respectively) compared to NE+/NS (61.5%). In summary, DL heifers had the greatest BW and BCS at AI, percentage cycling, and AI pregnancy rate. However, this strategy did not result in differing overall pregnancy rates between DL, E+/S, and E+/LS and there were no differences in milk production, rebreeding reproductive performance, and calf performance between all treatments. Finally, the poorest AI and overall pregnancy rates of the NE+/NS heifers suggests this is not a viable development strategy for fall-born heifers.


2015 ◽  
Vol 27 (1) ◽  
pp. 97
Author(s):  
G. A. Pessoa ◽  
A. P. Martini ◽  
J. M. Trentin ◽  
D. R. Dotto ◽  
H. L. D. Neri ◽  
...  

The aim of this study was to compare 3 methods for synchronization of ovulation in anestrous beef cows. The hypothesis of this study was to determine whether low doses of hCG has superior efficacy to cypionate to induce ovulation in anestrous cows and provide higher pregnancy rate in oestrus-synchronization programs. Synchronization of ovulation and conception rate to timed AI (TAI) were evaluated in anestrus Bos taurus taurus suckling beef cows 45 ± 15 days postpartum and with body condition score of 2.9 (1 to 5) maintained in a native pastured system in the south of Brazil. Females were evaluated with ultrasound on the Day 0 (D0) of the protocol (Day 0), day 8 (D8), immediately before TAI (D10), and 7 days after TAI (Day 17). All cows were synchronized with an intravaginal progesterone-releasing device (IPRD; 0.75 g of progesterone, Prociclar®, Hertape Calier Animal Health, Juatuba, Brazil) and 2 mg IM of oestradiol benzoate (EB; Benzoato HC®) on D0. On Day 8, the IPRD was removed and 150 μg of D (+) cloprostenol (Veteglan Luteolytic®), and 25 IU IM FSH/LH (Pluset®) were administered. Females of the EC (n = 84) group received 1 mg IM of oestradiol cypionate (EC; Cipionato HC®). Females on D8 of the hCG (n = 81) group received 500 IU IM of hCG (Vetecor®, Hertape Calier) at the time of TAI. The females of the EC + hCG group (n = 83) received both treatments. All cows were submitted to TAI 54 h after withdrawal of IPRD. A part of the cows (n = 102) had the ovulation evaluated every 12 h from the withdrawal of IPRD [EC (n = 34), hCG (n = 34), and hCG + EC (n = 33)]. Statistical analysis was performed using SAS PROC GLIMMIX. The dominant follicle diameter (FD) on Day 8 (8.7 ± 0.2, 8.8 ± 0.2, 8.6 ± 0.2) did not differ between treatments EC, EC + hCG, or hCG (P = 0.79). However, the FD on D10 was higher (P = 0.001) for cows treated with hCG (12.9 ± 0.3) compared with cows from the EC (11.3 ± 0.2) or EC + hCG group (11.8 ± 0.2). The interval (h) between the withdrawal of IPRD and ovulation was lower (P = 0.01) for the hCG group, (71.2 ± 1.7) compared with the groups treated with EC or EC + hCG (76.6 ± 2.18 and 74.2 ± 1.65), respectively. The ovulation rate did not differ (P = 0.61) among the EC (85.2%, 29/34), hCG (91.1%, 31/34), or EC + hCG groups (90.9%, 30/33). Corpus luteum diameter (mm) was higher (P = 0.04) on D17 for the hCG-treated group (21.4 ± 0.3) compared with others treatments (EC = 19.1 ± 0.8 or EC + hCG = 20.4 ± 0.8). However, the plasma progesterone levels on D17 were EC = 2.0 ± 0.1, hCG = 2.4 ± 0.1, and EC + hCG = 2.3 ± 0.1 ng mL–1 (P = 0.19), and the conception rate on the 28th day after TAI (EC = 43.0%; hCG = 47.0%, and EC + hCG = 48.8%; P = 0.76) was also similar. The hCG determined smallest ovulation interval, but similar rates of pregnancy were observed with both treatments.


2019 ◽  
Vol 31 (1) ◽  
pp. 129
Author(s):  
J. B. S. Borges ◽  
D. S. V. Luiz ◽  
P. R. L. Aguiar ◽  
C. G. B. Berlitz ◽  
G. S. Velho ◽  
...  

Beef heifers that conceive early during their first breeding season calve earlier, wean heavier calves, and tend to continue doing so throughout the rest of their reproductive lives. Otherwise, under extensively managed systems in Southern Brazil, only a variable proportion of 2-year-old beef heifers reach puberty before the start of the breeding season. The aim of this study was to evaluate a protocol to induce puberty in peripubertal beef heifers using injectable progesterone (P4) and oestradiol cypionate (EC) to improve pregnancy per AI (P/AI) in fixed-time AI (FTAI) programs. A total of 311 Angus heifers, 24±3 months of age, 3.1±0.3 body condition score (1=emaciated; 5=obese), and 328±41kg of body weight (BW), were examined before FTAI protocol (Day −24) by transrectal ultrasonography to determine reproductive tract score (RTS; 1=prepubertal; 5=pubertal). Prepubertal heifers (RTS 1; 12.8%, 40/311) were excluded from the study. Heifers with RTS=2 (n=271) were assigned to 2 groups: P4 (RTS 2 or 3, n=100) and cyclic (RTS 4 and 5, n=171). Peripubertal heifers were treated on Day −24 with 150mg of injectable P4 IM (Sincrogest® injectable, Ourofino Animal Health, Cravinhos, Brazil) and on Day −12 received 1mg of EC IM (Sincro CP®, Ourofino Animal Health). Cyclic heifers received no hormonal treatment before FTAI protocol. On Day 0, heifers in the P4 group were evaluated again to determine RTS, body condition score, and BW. Induction of puberty was defined by the presence of a follicle &gt;8.5mm (RTS=4) or a corpus luteum (RTS=5). All heifers received an FTAI protocol consisting of 2mg of oestradiol benzoate IM (Sincrodiol®, Ourofino Animal Health) and an intravaginal progesterone implant (1 g; Sincrogest®, Ourofino Animal Health). On Day 8, heifers had implants removed and were injected with 1mg of EC and 500µg of sodium cloprostenol IM (Sincrocio®, Ourofino Animal Health). After 48h, all heifers received an FTAI. Thirty days after AI, the diagnosis of gestation was performed by ultrasonography. Statistics included chi-square and linear regression with coefficients analysed as independent variables, considering a significant P-value of 0.05. On Day 0, percentages of RTS 5, 4, and 3 in the P4 heifers were 37, 27, and 36%, respectively, resulting in 64% of puberty induction. The P/AI was similar (P=0.14) in P4-treated and cyclic heifers (59v. 50.3%, respectively). The regression analysis presented a linear and positive effect of BW on P/AI (P&lt;0.001) in cyclic heifers and a positive influence of BW on RTS (P=0.05) and RTS on P/AI (P=0.008) in P4-treated heifers. The application of RTS system associated with P4 treatment of peripubertal heifers before FTAI protocol improved ovarian activity and might have contributed to higher P/AI, comparable with that achieved in cyclic heifers.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 173-173
Author(s):  
Richard A Ehrhardt ◽  
Jordan Moody ◽  
Barbara Makela ◽  
Veiga-Lopez Almudena

Abstract Productivity and seasonal constraints in lamb production can be overcome with accelerated systems. Accelerated adoption, however, has been limited by seasonal constraints in reproduction. Preliminary data suggest that periconceptional nutrition affects reproduction in the sub-optimal, long day breeding season. To evaluate this, we randomly assigned prolific, multiparous, Polypay x Dorset ewes at mid-lactation to nutritional treatments (5) over 2 periods: last 30 days of lactation (LACT) followed by the flushing period (FLUSH) consisting of the 21-day pre-breeding and the 34-day breeding period (total 55 days). During LACT, ewes were fed 100% (C), 70% (Low), or 150% (High) of late lactation energy requirements according to lamb rearing status (single or multiple). At FLUSH, Low or High ewes were fed either 70% (Low) or 150% (High) of energy requirements for maintenance (C ewes fed at 100% maintenance during FLUSH). This design was repeated over two seasons: optimal (SHORT day, n = 117) and suboptimal (LONG day, n = 108) breeding periods at 42.73°N and 84.5°W. Ewes were exposed to Dorset rams in 5 pens at 4–5% rotated every 12h. Reproductive outcomes were examined by ultrasound (transabdominal for litter size and conception rate; transrectal for early pregnancy loss). GLM analysis revealed changes in bodyweight and body condition score (BCS) consistent between seasons (Table 1). Regardless of dietary treatment, litter size was higher in SHORT (P &lt; 0.05), while embryonic loss tended to be higher in LONG season (P = 0.08). Within season, treatment had no effect on reproductive outcomes, however prolonged undernutrition (Low-Low) reduced conception rate in LONG vs. SHORT season (100 vs.78%; P &lt; 0.05). These results indicate that 1) high conception rates in LONG are attainable with natural mating and 2) chronic undernutrition hampers conception during long day periods. Finally, traditional flushing protocols are inadequate to invoke an optimal ovulation response regardless of season in accelerated systems.


Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 800
Author(s):  
Luis Javier Montiel-Olguín ◽  
Felipe J. Ruiz-López ◽  
Miguel Mellado ◽  
Eliab Estrada-Cortés ◽  
Sergio Gómez-Rosales ◽  
...  

Management and production characteristics impact conception rate to first service (CR1S) in small-scale dairy farms, but the impact of body condition score (BCS) and milk production levels on cows’ fertility is unknown. Our objective is to determine the effect of BCS and milk production on CR1S in small-scale dairy farms of western Mexico. Logistic regression models are used to determine the effect of BCS (at calving and first service), 60-d and 305-d milk production, protein and fat production, lactation number, and days at first service on CR1S. BCS at calving does not affect CR1S in cows with three or more lactations (39.5%; p > 0.1). However, first-lactation cows with BCS < 3.0 at calving and second lactation cows with BCS ≤ 2.5 at calving have higher CR1S (63.2 and 67.9%, respectively; p < 0.1). This result is perhaps due to reduced milk production, which leads to lower metabolic stress. BCS ≤ 2.5 at calving is associated (p < 0.05) with a reduced milk yield, explaining partially the observed higher CR1S in these groups. Cows with BCS ≤ 2.5 at first service in the higher quartile of 60-d milk production (≥ 28kg/day) show lower CR1S (23.9 and 51.1%, respectively; p < 0.01). In conclusion, BCS at calving and at first service, 60-d milk production, and lactation number are factors associated with CR1S.


2005 ◽  
Vol 10 (1) ◽  
Author(s):  
M.B. MOLENTO ◽  
M. ALMEIDA ◽  
E. GUTERRES ◽  
J. ROMAN ◽  
F. FREITAS ◽  
...  

A severidade das infecções parasitárias depende em grande parte da quantidade e do tipo de parasita presente e do estado nutricional dos animais. O objetivo deste experimento foi associar o efeito da suplementação protéica e pastagem de alta qualidade com diferenças na resposta ao desafio parasitário em 36 novilhas de oito meses, naturalmente infectadas, durante 120 dias. Grupo I: pastagem (Avena strigosa e Lolium multiflorum); Grupo II: pastagem e níveis crescentes de suplemento (Triticum aestivum, farelo de trigo) de 0,3 a 1,5% do peso vivo (PV); Grupo III: pastagem e suplemento a 0,9% do PV e Grupo IV: pastagem e níveis decrescentes de suplemento. O grau de infecção parasitária foi determinado através da contagem de ovos por grama de fezes (OPG) e coprocultura. Animais com OPG acima de 600 foram tratados com levamisole (Ripercol®, Fort Dodge). A coprocultura revelou os gêneros Cooperia spp. e Trichostrongylus spp. O ganho de peso, o escore corporal e o nível parasitário foi semelhante entre os grupos. Foram administradas 24 doses de anti-helmíntico com baixo grau de repetibilidade dos animais. Determinou-se que o tratamento seletivo pode ser aplicado quando os animais dispõem de pastagem de alta qualidade e com baixo desafio parasitário, independente de suplementação alimentar. Naturally infected beef heifers with gastrintestinal parasites – epidemiology and selective treatment Abstract The severity of parasite infections depends in part by the number and the parasite species as well as on the nutritional status of the host. The objective of this work was to determine the association between protein supplement and good quality pasture with parasite challenge infection in 36 naturally infected, 8 months old beef heifers during 120 days. Group I: pasture (Avena strigosa and Lolium multiflorum); Group II: pasture plus increasing levels of supplement (Triticum aestivum, wheat bran) from 0.3 to 1.5% of live weight (LW); Group III: pasture plus 0.9% LW of supplement; Group IV: pasture plus decreasing levels of supplement. Parasite infection was determined through fecal egg counts (EPG) and coproculture. All animals with EPG above 600 were treated with levamisole (Ripercol®, Fort Dodge). Coproculture displayed Cooperia sp. and Trichostrongylus sp. Weight gain, body condition score and parasite levels were similar among all groups. Twenty-four doses of the anthelmintic were administered with low animal repeatability. It was determined that the selective treatment may be used when animals have access to good quality pasture and a low parasite challenge, independently of the supplement offered.


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