Comparative Histomorphochemical Studies on Uropygial Gland of Kadaknath and White Leghorn Breeds of Poultry

2017 ◽  
Vol 13 (02) ◽  
Author(s):  
S. Kanasiya ◽  
S. K. Karmore ◽  
S. K. Deshmukh ◽  
R. K. Barhaiya ◽  
S. K. Gupta
Keyword(s):  
Characteristic Feature ◽  
Periodic Acid ◽  
Elastic Fibers ◽  
Moderate Activity ◽  
Uropygial Gland ◽  
White Leghorn ◽  
Preen Gland ◽  
Central Cavity ◽  
Tissue Samples ◽  
Melanin Pigmentation

The present study was conducted on ten each uropygial glands of 6 to 8 months old healthy Kadaknath and White Leghorn breeds of poultry. The tissue samples were stained by H and E for normal histological structures, Verhoeff’s stain for collagen and elastic fibers, PAS (Periodic Acid Schiff’s) for glycogen and Alcian Blue PAS method for acid mucopolysaccharides. The uropygial gland in Kadaknath and White Leghorn was composed of two lobes. Each lobe had a single duct and these ducts were joined together by isthmus. The thickness of capsule was more in White Leghorn than Kadaknath breed. The lumen of tubules showed higher concentration of secretary product in Kadaknath breed. Numbers of tubules were higher in Kadaknath. No aggregation of lymphocytes was found in the preen gland of White Leghorn, whereas in Kadaknath, there was large number of lymphocytes aggregation alongwith lymphatic nodules. Melanin pigmentation was the characteristic feature of Kadaknath which was found towards the central cavity and in between ductules. The capsule of White Leghorn showed intense PAS activity, while moderate activity was found in Kadaknath breed of poultry. Intense ACPase reaction was noticed in capsule of uropygial gland of Kadaknath and White Leghorn breeds of poultry.

scholarly journals Distribution of Melanin Pigmentation in 33 Organs of Thai Black-Bone Chickens (Gallus gallus domesticus)

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 777
Author(s):  
Korakot Nganvongpanit ◽  
Piyatida Kaewkumpai ◽  
Varankpicha Kochagul ◽  
Kidsadagon Pringproa ◽  
Veerasak Punyapornwithaya ◽  
...  
Keyword(s):  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Melanin Pigment ◽  
Gallus Gallus Domesticus ◽  
Tissue Samples ◽  
Melanin Pigmentation ◽  
Chicken Breed ◽  
Brachial Vein ◽  
The Brain ◽  
Pigment Accumulation

The black-bone chicken (Gallus gallus domesticus) is a breed of chicken that is commonly found in Thailand. This breed is known for having a number of black colored organs. Consumers have been notably attracted to the black-bone chicken breed for the characteristic darkness that is observed in many of its organs. However, the degree of darkness in all organs of the black-bone chicken is still in question. Importantly, there have not yet been any published reports on the distribution of melanin pigment in the organs of the black-bone chicken. This research study aims to examine the distribution of the melanin pigment in 33 organs of the Thai black-bone chicken. Ten black-bone chickens (five male, five female) were included in this study. Thirty-two organs including the brain, spinal cord, sciatic nerve, larynx, trachea, syrinx, lungs, heart, pericardium, aorta, brachial vein, kidney, cloaca, oviduct, testis, gastrocnemius muscle, femur, tongue, esophagus, crop, proventriculus, gizzard, duodenum, jejunum, ileum, cecum, pancreas, liver, gall bladder, omentum, abdominal fat, spleen, and skin were examined in this study. Histological sections taken from tissue samples of each of these organs were studied. The findings revealed that the presence of the melanin pigment was not significantly different (p > 0.005) between male and female specimens. Notably, the liver was the only organ in which the melanin pigment had not accumulated. Consequently, there was not a uniform pattern of melanin pigment accumulation throughout the organs of the chickens. The melanin pigment was present in all of the tissue layers of most organs, while the melanin pigment was found in only specific layers of some of the organs. In conclusion, the distribution of melanin pigmentation in the organs of each of the animals in this study was found to be different. However, in some tissue samples, such as those obtained from the liver, no accumulation of the melanin pigment was observed.

scholarly journals Histologic Aspect of the Curved Vomerine Mucosa in Cleft Lip and Palate

2021 ◽  
pp. 105566562110314
Author(s):  
Benito K. Benitez ◽  
Andrzej Brudnicki ◽  
Prasad Nalabothu ◽  
Jeannette A. von Jackowski ◽  
Elisabeth Bruder ◽  
...  
Keyword(s):  
Nasal Mucosa ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Squamous Metaplasia ◽  
Periodic Acid ◽  
Surgical Techniques ◽  
Basal Cells ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Nasal Floor

Background: Common surgical techniques aim to turn the entire vomerine mucosa with vomer flaps either to the oral side or to the nasal side. The latter approach is widely performed due to the similarity in color to the nasal mucosa. However, we lack a histologic description of the curved vomerine mucosa in cleft lip and palate malformations. Methods: We histologically examined an excess of curved vomerine mucosa in 8 patients using hematoxylin–eosin, periodic acid–Schiff, Elastin van Gieson, and Alcian blue stains. Tissue samples were obtained during surgery at 8 months of age. Results: Our histological analysis of the mucoperiosteum overlying the curved vomer revealed characteristics consistent with those of an oral mucosa or a squamous metaplasia of the nasal mucosa, as exhibited by a stratified squamous epithelium containing numerous seromucous glands. Some areas showed a palisaded arrangement of the basal cells compatible with metaplasia of respiratory epithelium, but no goblet cells or respiratory cilia were identified. Abundant fibrosis and rich vascularity were present. Conclusion: The vomer mucosa showed no specific signs of nasal mucosa. These findings should be considered in presurgical cleft orthopedics and palatal surgery for further refinement. Shifting the vomer mucosa according to a fixed physiologic belief should not overrule other important aspects of cleft repair such as primary healing and establishing optimal form and function of palatal roof and nasal floor.

Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis

2000 ◽  
Vol 88 (3) ◽  
pp. 1022-1028 ◽  
Author(s):  
Lu Wang ◽  
Kenneth L. Pinder ◽  
Joel L. Bert ◽  
Mitsushi Okazawa ◽  
Peter D. Paré
Keyword(s):  
Mechanical Properties ◽  
Volume Fraction ◽  
Mechanical Load ◽  
Morphological Structure ◽  
Longitudinal Direction ◽  
Smooth Muscle Contraction ◽  
Elastic Fibers ◽  
Tissue Samples ◽  
Mucosal Membrane ◽  
Simple Geometry

Folding of the airway mucosal membrane provides a mechanical load that impedes airway smooth muscle contraction. Mechanical testing of rabbit tracheal mucosal membrane showed that the membrane is stiffer in the longitudinal than in the circumferential direction of the airway. To explain this difference in the mechanical properties, we studied the morphological structure of the rabbit tracheal mucosal membrane in both longitudinal and circumferential directions. The collagen fibers were found to form a random meshwork, which would not account for differences in stiffness in the longitudinal and circumferential directions. The volume fraction of the elastic fibers was measured using a point-counting technique. The orientation of the elastic fibers in the tissue samples was measured using a new method based on simple geometry and probability. The results showed that the volume fraction of the elastic fibers in the rabbit tracheal mucosal membrane was ∼5% and that the elastic fibers were mainly oriented in the longitudinal direction. Age had no statistically significant effect on either the volume fraction or the orientation of the elastic fibers. Linear correlations were found between the steady-state stiffness and the quantity of the elastic fibers oriented in the direction of testing.

scholarly journals Experimental Test of the Importance of Preen Oil in Rock Doves (Columba Livia)

The Auk ◽  
2003 ◽  
Vol 120 (2) ◽  
pp. 490-496 ◽  
Author(s):  
BrettR. Moyer ◽  
Alex N. Rock ◽  
Dale H. Clayton
Keyword(s):  
Experimental Test ◽  
Columba Livia ◽  
Uropygial Gland ◽  
Preen Gland ◽  
In Vivo Experiments ◽  
Preen Oil ◽  
Bacteria And Fungi

Abstract Most species of birds have a uropygial gland, also known as a preen gland, which produces oil that birds spread through their plumage when preening. The plumage of waterfowl deprived of uropygial oil becomes brittle and is subject to breakage. For other groups of birds, however, the importance of preen oil remains unclear. Previous workers have argued that preen oil may serve little or no function in Columbiforms (pigeons and doves). We tested that assertion by removing uropygial glands from Rock Doves (Columba livia) and assessing their plumage condition after several months. The results of that experiment showed significant degradation of plumage in the absence of oil. Our results are the first rigorous demonstration that preen oil is important for plumage condition in nonwaterfowl. We tested one possible function of preen oil—that it has insecticidal properties and that reduction in plumage condition on birds without glands is due to an increase in ectoparasites. We tested that hypothesis for feather-feeding lice (Phthiraptera:Ischnocera) using both in vitro and in vivo experiments. Lice raised in an incubator died more rapidly on feathers with preen oil than on feathers without oil, which suggests that preen oil may help combat lice. However, removal of the preen gland from captive birds had no significant effect on louse loads over the course of a four-month experiment. Although the results of our in vivo experiments suggest that preen oil may not be an important defense against lice, further experiments are needed. We also consider the possibility that preen oil may protect birds against other plumage-degrading organisms, such as bacteria and fungi.

scholarly journals Superficial Morphea: Clinicopathological Characteristics and a Novel Therapeutic Outcome to Excimer Light Therapy

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Al-Sadat Mosbeh ◽  
Soha Aboeldahab ◽  
Mohamed El-Khalawany
Keyword(s):  
Light Therapy ◽  
Clinicopathological Characteristics ◽  
Clinicopathological Features ◽  
Therapeutic Outcome ◽  
Hcv Infection ◽  
Localized Scleroderma ◽  
Elastic Fibers ◽  
Reticular Dermis ◽  
Melanin Pigmentation ◽  
Therapeutic Modalities

Introduction. Superficial morphea (SM) is an uncommon entity that was described in the literature without definitive correlation to localized scleroderma (LS) or other atrophoderma diseases. Aim. To demonstrate the clinicopathological features of SM and evaluate the efficacy of different therapeutic modalities in its management. Patients and methods. A total of 28 patients with SM were studied during the period from 2010 to 2015. Clinicopathological features and therapeutic outcomes were recorded and analyzed. Results. Clinically, SM was predominant in females (71.4%) with an average onset at 33 years of age and an average duration of 15 months. It was commonly presented as asymptomatic, darkly pigmented, and multiple and slightly indurated patches. The lesions were mostly ill-defined, large-sized, and located more on the trunk. Histologically, thickening of collagen fibers was observed either localized to the papillary dermis only (38.9%) or extended into the upper reticular dermis (61.1%). Elastic fibers were generally diminished in the upper reticular dermis while the number of fibroblasts and basal melanin pigmentation were increased in the majority of cases (92.9% and 96.4%, respectively). The most commonly associated diseases were diabetes mellitus (50%) and hepatitis C virus (HCV) infection (42.8%), and their incidence was significantly higher than that in patients with LS. Excimer light showed promising effective results in the treatment of most cases (78.9%) while the response to other modalities such as topical corticosteroid alone or in combination with tacrolimus or treatment with UVA1 alone was less effective (7.1%, 23.1%, and 5%, respectively). Conclusion. Our results proposed that SM is a distinctive clinicopathological variant and not a stage in the spectrum of LS. The novel response of SM to excimer light and not for UVA1 therapy also suggests the different therapeutic outcome of SM from LS. Although SM has a significant association with DM and HCV infection, they seem not to affect the course of the disease.

Pathology and prevalence of hepatic lesions of Azeri buffaloes (bubalus bubalis) slaughtered at Urmia Abattoir, Northwest Iran

2020 ◽  
Author(s):  
Reza Alizadeh ◽  
Amir Amniattalab
Keyword(s):  
Bile Duct ◽  
Periodic Acid ◽  
Negative Influence ◽  
Fatty Change ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Hepatic Congestion ◽  
Significant Difference ◽  
Northwest Iran ◽  
Hepatic Lesions

This study was carried out for the evaluation of the prevalence rate and the pathology of various lesions in the confiscated livers of slaughtered Azeri buffaloes at Urmia Abattoir, Northwest Iran. Livers of 306 Azeri buffaloes were inspected grossly during five months according to their color and consistency changes in Urmia Abattoir. Among the inspected carcasses, livers of 28 buffaloes had various lesions. The collected tissue samples of confiscated livers were placed in 10% buffered formalin as a fixative solution in order to prepare pathologic sections with a thickness of 6 μm. The sections were stained by haematoxylin and eosin (H&E) and periodic acid schiff (PAS) methods. The most common pathologic changes in the confiscated buffalo livers were fatty change (46.4%), hepatitis and cholangitis (42.9%), and bile duct hyperplasia (39.3%) respectively. The results of this study have shown that there was a significant difference (p<0.05) between hepatic congestion and sex. In addition, there was no significant difference (p>0.05) between hepatic lesions and age. The results of this study have revealed that fascioliasis has an important role in the creation of some hepatic lesions such as bile duct hyperplasia, fatty change, and hepatitis in buffaloes. Since various hepatic lesions can reduce buffalo productions and has negative influence on animal reproduction, it is necessary to pay more attention to accurate performing of antiparasitic program and the evaluation of possible role of poisonous plants, heavy metals, and mycotoxins in the generation of hepatic lesions of buffaloes.

A comparison of acute vascular damage caused by ADAPT versus a stent retriever device after thrombectomy in acute ischemic stroke: a histological and ultrastructural study in an animal model

2016 ◽  
Vol 9 (8) ◽  
pp. 743-749 ◽  
Author(s):  
S Peschillo ◽  
F Diana ◽  
J Berge ◽  
P Missori
Keyword(s):  
Arterial Wall ◽  
Elastic Fibers ◽  
Cell Alignment ◽  
Internal Elastic Lamina ◽  
Endothelial Lining ◽  
Tissue Samples ◽  
First Pass ◽  
Stent Retrievers ◽  
Ultrastructural Damage ◽  
Elastic Lamina

BackgroundIt has been amply demonstrated that endovascular procedures can be successful treatment for stroke, both in terms of revascularization and clinical outcome. There is not, however, a published comparison of any histological or ultrastructural damage to the vessels that may be caused by a direct aspiration first pass technique (ADAPT) or stent retrievers (SR) used in these procedures. This study analyses and compares acute damage to the arterial wall caused by ADAPT or SR.Material and methodsDamage to the walls of swine extracranial arteries was evaluated after ADAPT with the Penumbra system or thrombectomy with an SR (Solitaire 6×30). The procedures were performed after injecting thrombi into the selected arteries (arteries with diameters similar to those of the human internal carotid artery and first segment of the middle cerebral artery). After the procedures, the animal was euthanized and 12 arterial samples were obtained for analysis by optical and electronic microscopy.ResultsTissue samples from the vessels treated with SR showed almost complete loss of endothelium, thickening of the internal elastic lamina, and degeneration of the elastic fibers of the bordering lamina media and adventitia. In contrast, tissue samples of the vessels treated with ADAPT had a clear integral internal elastic lamina and uninterrupted endothelial lining, although cell alignment was altered and there were surface lacerations due to manipulation of the samples.ConclusionsBoth techniques caused acute damage to the vessel walls, however, thrombectomy with SR appeared to be more harmful to all layers of the arterial wall, particularly the endothelium.

scholarly journals Comparative Anatomical Studies on Ductus Venosus in Fetuses of Domestic Ruminants

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Pamela Bejdić ◽  
Amel Ćutuk ◽  
Amer Alić ◽  
Benjamin Čengić ◽  
Rizah Avdić ◽  
...  
Keyword(s):  
Histological Examination ◽  
Umbilical Vein ◽  
Circulatory System ◽  
Fetal Tissue ◽  
Ductus Venosus ◽  
Elastic Fibers ◽  
Silver Stain ◽  
Tissue Samples ◽  
Domestic Ruminants ◽  
Hematoxylin And Eosin

AbstractThe study has aimed to investigate and determine the anatomical position, shape, size, and histological features of the ductus venosus, and its role as a shunt in the fetal circulatory system in domestic ruminants. The research was conducted on 19 bovine, 11 sheep and 5 goat fetuses, aborted at the late stage of pregnancy or deceased just after delivery. The general anatomy of the ductus venosus was investigated by in-situ dissection of the corrosive cast obtained by injection of 25% solution of Vinylite mass through the umbilical vein. For histological examination, the fetal tissue samples were stained with Hematoxylin and Eosin, Masson’s trichrome, Verhoeff-Van Gieson and Gomori's silver stain. The results showed that ruminant fetal ductus venosus is a curved, trumpet-shaped vessel, situated in the central part of the liver, above the porta hepatis. Its ventral part is constricted in the form of an isthmus, having a prominent lip-like thickening at the junction with the portal sinus. Histological examination showed the dominant presence of collagen and elastic fibers in its tunica media, with thin bands of smooth muscle fibers oriented in a longitudinal and circular direction indicating ability for vasoconstriction and vasodilatation.

scholarly journals Identification of Brachyspira spp. in the cecum of broiler chickens using histology and in situ diagnostic assays

2021 ◽  
Vol 42 (5) ◽  
pp. 2813-2824
Author(s):  
Monica Regina de Matos ◽  
◽  
Aline Patrícia Grzegozevski ◽  
Alessandra da Cruz ◽  
Arthur Colombari Cheng ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Ffpe Tissue ◽  
Economic Losses ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Rabbit Polyclonal Antibody ◽  
Formalin Fixed Paraffin Embedded

The genus Brachyspira corresponds to the group of bacteria formerly classified into the genus Serpulina and includes several commensal and pathogenic intestinal spirochetes that affect pigs, poultry, and other animal species, including humans. In birds, some pathogenic species of this genus causes a condition known as avian intestinal spirochetosis, which remains underdiagnosed, thereby causing serious economic losses. Brachyspira is a fastidious organism that necessitates the employment of fast and efficient identification techniques. The aim of this study was to identify Brachyspira spp. using histology, immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in formalin-fixed paraffin embedded (FFPE) tissue samples from the cecum of commercial poultry. Samples were collected from 129 birds aged between 35 and 45 days from commercial broiler farms. For evaluation, routine histology processing (H&E) and the histochemical technique, periodic acid–Schiff (PAS) were done. Additionally, FFPE tissue samples were evaluated for FISH and IHC. The histological lesions were analyzed and graded after H&E staining, and the goblet cells were counted and compared using PAS staining with the positive and negative samples obtained through FISH and IHC. For FISH, probes labeled with Brachyspira spp., B. pilosicoli, B. hyodysenteriae, and B. intermedia were used, whereas rabbit polyclonal antibody specific for Brachyspira spp. was used for IHC. Of 129 samples, 82 were positive with IHC and 86 were positive with FISH. The samples positive for the genus Brachyspira in the FISH technique were tested for B. pilosicoli, B. hyodysenteriae, and B. intermedia in which 56 were positive for B. pilosicoli, 75 for B. hyodysenteriae and 80 for B. intermedia. There was an increase in goblet cells in the samples positive for FISH and IHC. The techniques used were effective and gave corresponding results, thus serving as a fast and efficient tool for diagnosis.

scholarly journals Mucosubstances in the porcine gastrointestinal tract: Fixation, staining and quantification

2019 ◽  
Vol 63 (2) ◽  
Author(s):  
Juliane Rieger ◽  
Barbara Drewes ◽  
Hana Hünigen ◽  
Johanna Plendl
Keyword(s):  
Tissue Section ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Staining Procedure ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Feeding Experiments ◽  
Neutral Mucin ◽  
Electron Microscopical ◽  
Triple Staining

Mucins are of great interest in intestinal research and histochemical methods are often employed to identify them. Since it is in the nature of mucins that they are “hard to hold onto” once they come into contact with water, a frequently used medium in histochemistry, there are a number of challenges that may decrease diagnostic accuracy. As the outcome of methods published for microscopic detection of mucosubstances proved to be unsatisfactory in our hands, the aim was the establishment of a reliable and reproducible protocol. Tissue samples were available from pig feeding experiments. In the present study, we focus on a fixation / staining procedure without making comparisons between differently fed pigs. Several fixation and staining procedures were evaluated for their use in semiautomatic quantification and quality assessment of different mucus fractions simultaneous on one tissue section. Cryostat sectioning, subsequent fixation steps with heat, ethanol and modified Bouin’s solution, followed by triple staining with high iron diamine, alcian blue and periodic acid-Schiff turned out to be the best method to identify sulfomucin, sialomucin and neutral mucin simultaneous on one tissue section. This methodology resulted in very good morphology of goblet cells with intact mucin containing vesicles within the cells, which was comparable to ultrastructural electron microscopical observations. Semiautomatic quantification of different mucins was possible. In conclusion, reliable mucus quantification and assessment of mucus quality requires strictly tested procedures. According to our experience, the most important aim after cryosectioning is fast fixation of the mucosubstances, which requires a combination of different fixation steps.

Sign in / Sign up

Export Citation Format

Share Document