scholarly journals ANTINUCLEAR ANTIBODIES IN PATIENTS WITH UNEXPLAINED RECURRENT ABORTIONS

2017 ◽  
Vol 10 (8) ◽  
pp. 256 ◽  
Author(s):  
Anupriya Asaithambi ◽  
Manjula Gunasekaran ◽  
Manivelan S ◽  
Palaniappan Nainar
Keyword(s):  
Pregnant Women ◽  
Antinuclear Antibodies ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Substrate ◽  
Simple Alternative ◽  
History Of ◽  
Recurrent Abortions ◽  
Control Study ◽  
Indirect Fluorescence Antibody

Objective:This study aims to determine the prevalence of antinuclear antibodies in pregnant women with bad obstetric history (BOH) against healthy multigravid women in and around Tirunelveli districtMethods: This is a case-control study comprising 60 antenatal women with BOH against 60 multiparous pregnant women with no history of abortions. Antinuclear antibodies (ANA) were detected using indirect immunofluorescence with Hep-2 cell substrate, and enzyme-linked immunosorbent assay (ELISA).Results: Among BOH cases 19 (82.6%), 18 (78.26%) were positive by ELISA and indirect fluorescence antibody test (IFAT) method, respectively. Among controls, 4(17.39%) and 5(21.73%) individuals were positive by ELISA and IFAT methods, respectively. Of the 18 positives, homogenous pattern was most common followed by anticentromere pattern, fine speckled and coarse speckled patternConclusion: IFAT is considered to be gold standard in the diagnosis of autoimmune disorders, but ELISA appears to be a suitable simple alternative for testing rheumatological disorders.

scholarly journals Family history of hypertension increases risk of preeclampsia in pregnant women: a case-control study

2016 ◽  
Vol 35 (3) ◽  
pp. 181 ◽  
Author(s):  
Mulualem Endeshaw ◽  
Fantu Abebe ◽  
Melkamu Bedimo ◽  
Anemaw Asrat ◽  
Abebaw Gebeyehu ◽  
...  
Keyword(s):  
Family History ◽  
Pregnant Women ◽  
Case Control Study ◽  
Multiple Logistic Regression Analysis ◽  
Multiple Pregnancy ◽  
Case Control ◽  
Outcome Variable ◽  
History Of ◽  
Family History Of Hypertension ◽  
Control Study

Background <br />Preeclampsia is one of the leading causes of maternal and fetal morbidity and mortalities worldwide. Despite extensive research, the underlying cause of preeclampsia remains poorly understood. This study aimed to offer compelling evidence on the important risk factors of preeclampsia in Amhara region, Ethiopia.<br /><br />Methods<br />A case control study was conducted in public health facilities of Bahir Dar city from September 2014 to January 2015. A total of 453 (151 cases and 302 controls) pregnant women were enrolled in this study. Hemoglobin level and urinary tract infection (UTI) status were collected from clinical notes. Oral examination was performed by a dentist for detection of periodontal diseases. Univariate and multiple logistic regression analysis was conducted to determine the relationship of all the independent variables with the outcome variable. A p-value &lt;0.05 was declared statistically significant.<br /><br />Result<br />Advanced maternal age (AOR=4.79;95% CI 1.031-22.18), family history of hypertension (AOR=11.16;95% CI 5.41-41.43), history of diabetes mellitus (AOR=6.17;95% CI 2.11-20.33), UTI in the current pregnancy (AOR=6.58;95% CI 2.93-14.73), failure to comply with iron and folic acid supplement during pregnancy (AOR=8.32;95% CI 3.35-20.62), lack of exercise (AOR=3.33;95% CI 1.35-8.17), multiple pregnancy (AOR=4.05;95% CI 1.57-12.27), anemia (AOR=4.19;95% CI 1.27-13.92), and periodontal disease or gingivitis (AOR =3.51;95% CI 1.14-10.83) were associated with preeclampsia.<br /><br />Conclusion <br />Family history of hypertension was the most dominant risk factor for preeclampsia in pregnant women. Encouraging pregnant women to have health seeking behavior during pregnancy would provide a chance to diagnose preeclampsia as early as possible.

scholarly journals Apelin 13 and Blood Pressure, Is there any Association in Pre-eclampsia? - A Case-control Study

2021 ◽  
Author(s):  
Rajeev Gandham ◽  
CD Dayanand ◽  
SR Sheela
Keyword(s):  
Blood Pressure ◽  
Pregnant Women ◽  
Gestational Age ◽  
Case Control Study ◽  
Case Control ◽  
Maternal Serum ◽  
Research Centre ◽  
Categorical Variables ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Study

Introduction: Pre-eclampsia is a pregnancy specific disorder, characterised by the onset of hypertension and proteinuria. Pre-eclampsia is the leading cause of maternal, perinatal morbidity and mortality. The exact cause of pre-eclampsia is not known clearly and needs to be explored. Aim: To evaluate the maternal serum apelin 13 levels among pre-eclampsia and healthy pregnant women and also, to find the association between apelin 13 and blood pressure. Materials and Methods: A case-control study was conducted between Department of Biochemistry and Department of Obstetrics and Gynaecology, RL Jalappa Hospital and Research Centre, Kolar, Karnataka, India. After approval from the Institutional Ethics Committee and written informed consent from study subjects, a total of 270 pregnant women were recruited for this study. Among them, 135 pre-eclamptic women were considered as cases and 135 normotensive healthy pregnant women served as controls. According to the pre-eclampsia severity, cases were grouped into mild (n=47) and severe pre-eclampsia (n=88). Blood samples were collected from all the study subjects and was analysed for apelin 13 by Enzyme Linked Immunosorbent Assay (ELISA) method. Maternal and foetal adverse outcomes were recorded. Results were expressed as mean±Standard Deviation (SD). Categorical variables were expressed in percentages. Spearman’s correlation was applied and p<0.05 was considered significant. Results: The mean gestational age was 36.66±3.69 weeks which was, significantly low in pre-eclamptic women compared with healthy pregnant women. BMI (26.94±3.81 kg/m2), systolic (157.82±15.14 mmHg), diastolic (101.68±11.02 mmHg) and Mean Arterial Pressure (MAP) (120.20±11.12 mmHg), pulse rate (88.14±5.82 bpm), Aspartate Transaminase (AST) (25.25±12.49 IU/L) and Alanine Transaminase (ALT) (19.01±10.95 IU/L) were significantly increased in pre-eclamptic women when compared with control group. Mean maternal serum apelin 13 (341.44±218.63 pg/mL) concentrations were significantly lower in pre-eclampsia compared with healthy pregnant women. Maternal serum apelin 13 concentrations were negatively correlated with Systolic Blood Pressure (SBP) (r = -0.196), Diastolic Blood Pressure (DBP) (r = -0.172) and MAP (r =-0.204). Adverse maternal outcomes such as epigastric pain 75 (55.55%), oedema 62 (45.92%) and persistent headache 35 (25.92%) were higher in pre-eclamptic group. Additionally, adverse foetal outcomes were more in pre-eclamptic cases including significantly decreased birth weight (2.40±0.65), babies requiring Neonatal Intensive Care Unit (NICU) admission were 54 (40%), preterm birth (≤37 wks) in 50 (37.03%), Respiratory Distress Syndrome (RDS) 31 (22.96%), Small for Gestational Age (SGA) in 4 (2.96%) and Intra Uterine Death (IUD) in 11 (8.14%) babies. Conclusion: It was concluded from the present study that there was low maternal serum apelin 13 concentrations in pre-eclampsia and had negative correlation with blood pressure, suggesting its potential role in the pathophysiology of pre-eclampsia.

Are Anti-β2 Glycoprotein 1 Antibodies Associated with Placenta-Mediated Pregnancy Complications? A Nested Case–Control Study

2018 ◽  
Vol 35 (11) ◽  
pp. 1093-1099 ◽  
Author(s):  
Leslie Skeith ◽  
Karim Abou-Nassar ◽  
Mark Walker ◽  
Tim Ramsay ◽  
Ronald Booth ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Pregnancy Complications ◽  
Case Control Study ◽  
Late Pregnancy ◽  
Case Control ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Antibody Positivity ◽  
Control Study

Background While anti-β2 glycoprotein 1 (anti-β2GP1) antibody positivity is included in the diagnostic criteria for antiphospholipid syndrome (APS), the association between anti-β2GP1 and the obstetrical complications of APS has been inconsistently reported and remains unclear. Objective We completed a case–control study nested within the Canadian Ottawa and Kingston (OaK) Birth Cohort to evaluate the association between anti-β2GP1 antibody positivity and placenta-mediated pregnancy complications. Study Design Five hundred cases were randomly selected among pregnant women who experienced any of the following independently adjudicated placenta-mediated pregnancy complications: preeclampsia, placental abruption, late pregnancy loss (≥ 12 weeks' gestation), and birth of a small-for-gestational age (SGA) infant < 10th percentile. Five hundred pregnant women without any placenta-mediated pregnancy complications were selected as controls. Stored blood samples were analyzed for the presence of anti-β2GP1 antibodies by enzyme-linked immunosorbent assay. Results Anti-β2GP1 immunoglobulin G (IgG) and/or immunoglobulin M (IgM) antibodies in titers ≥ 20 G/M units (> 99th percentile) were present in 24 of 497 (4.8%) of controls and 33 of 503 (6.6%) of cases. There was no significant difference between cases and controls for the composite outcome of any placenta-mediated pregnancy complications (odds ratio, 1.38, 95% confidence interval [CI], 0.8–2.37, p = 0.25). Conclusion Our results call into question the association between anti-β2GP1 antibodies and placenta-mediated pregnancy complications, with further research needed.

scholarly journals Hormonal monitoring of the function of the corpus luteum, trophoblast and placenta in pregnant women with a history of different types of infertility

2020 ◽  
pp. 6-12
Author(s):  
L.E. Tumanova ◽  
◽  
E.V. Kolomiets ◽  
Keyword(s):  
Surgical Treatment ◽  
Pregnant Women ◽  
Corpus Luteum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Group I ◽  
Rate Of Increase ◽  
History Of ◽  
Natural Conception ◽  
Β Hcg ◽  
Conservative And Surgical Treatment

Purpose — to investigate hormonal monitoring of the function of the corpus luteum, trophoblast and placenta in pregnant women with a history of various types of infertility. Materials and methods. We have studied hormonal parameters from 8 to 14 weeks of pregnancy in 420 women. The study of hormonal parameters was carried out in three groups (six subgroups): Group I — pregnant women with a history of endocrine infertility: Ia — 50 patients after IVF, Ib — 50 patients who became pregnant on their own after conservative and surgical treatment of infertility, but without IVF; Group II — pregnant women with a history of inflammatory infertility: IIa — 100 patients after IVF, IIb — 100 patients who became pregnant independently after conservative and surgical treatment of inflammatory infertility, but without IVF; Group III — pregnant women with a history of combined infertility, inflammatory genesis with endocrine, IIIa — 30 patients after IVF, IIIb — 30 patients who became pregnant on their own after conservative and surgical treatment of combined infertility, but without IVF. A study of the content of placental hormones in the dynamics of pregnancy at 7–10 and 11–14 weeks was carried out: estradiol (E2), progesterone, human chorionic gonadotropin (β-hCG) and pregnancy-associated plasmoprotein (PAPP-A). Determination of E2, progesterone was carried out by the enzyme-linked immunosorbent assay using standard kits of the Delfia system on a 1420 Victor 2 analyzer from Perken Elmer (USA). β-hCG and PAPP-A were determined by the immunochemiluminescent method using test systems manufactured by Siemens. Results. We carried out hormonal monitoring of the corpus luteum and trophoblast function and analyzed the results of fetal biochemical markers in 276 pregnant women. The data obtained indicate that in the period of 7–10 weeks of pregnancy, the concentration of progesterone was significantly higher in women after IVF relative to the indicators of patients with natural conception. At this stage of pregnancy, the level of progesterone did not depend on the form of infertility. Similar changes were observed with respect to estradiol levels. So the level of estradiol in pregnant women of 7–10 weeks during natural pregnancy was ≈5.0 nmol/L, while the same level of estradiol in pregnant women with one fetus after IVF was 8.4±1.1 nmol/L. The progesterone/estradiol ratio was virtually the same across the groups. The level of estradiol and progesterone in the blood of women at 11–14 weeks of gestation also practically did not differ, and did not depend on the form of infertility and the method of conception. It should be especially noted that at 11–14 weeks there was a decrease in the progesterone/estradiol ratio, which represents a progressive pronounced relative progesterone deficiency and hyperestrogenism in women with infertility. The indicators were especially low in pregnant women of groups I and III, who had endocrine and combined infertility in the anamnesis. We also investigated the indicators of β-hCG and PAPP-A in pregnant women 11–14 weeks. by groups, as classic markers of screening for congenital malformations of the fetus and the risk of complications of pregnancy. So the level of PAPP-A in pregnant women did not significantly differ in groups, both from the method of conception and the type of infertility in the anamnesis. The level of β-hCG in pregnant women 11–14 weeks of singleton pregnancy after IVF is significantly higher than in women with natural conception. The highest rates were in the group after combined infertility. Conclusions. The level of hormones: estradiol and progesterone in pregnant women after IVF at 7–10 weeks was higher than in women with a history of infertility during natural conception. Already at 11–14 weeks, the same indicators in the same groups did not differ. After natural conception, the rate of increase in estradiol significantly outpaced the increase in progesterone levels in pregnant women with a history of infertility. The concentration of PAPP-A in the first trimester in pregnant women after IVF did not significantly differ from those in women with natural conception. The content of β-hCG at 11–14 weeks in groups of pregnant women after IVF was 1.5–2 times higher. The highest rates were in pregnant women with a history of concomitant infertility. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of these Institutes. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: estradiol, progesterone, β-hCG, PAPP-A, pregnancy after infertility.

scholarly journals PREGNANCY OUTCOMES

2018 ◽  
Vol 21 (02) ◽  
pp. 347-353
Author(s):  
Manzoor Ahmad Naeem ◽  
Usra Naeem ◽  
Asif Hanif
Keyword(s):  
Risk Factors ◽  
Pregnant Women ◽  
Pregnancy Outcome ◽  
Pregnancy Outcomes ◽  
Case Control Study ◽  
Case Control ◽  
Hypertensive Disorder ◽  
Pregnant Females ◽  
History Of ◽  
Control Study

Introduction: Pregnancy is a normal physiological event but some pregnancyspecific or other medical conditions can cause maternal as well as fetal morbidities and evenmortalities. Among them, raised blood pressure during pregnancy adversely affects bothmaternal and fetal outcomes. Objectives: In this study, risk factors associated with hypertensivedisorders of pregnancy are explored and pregnancy outcomes of hypertensive women withnormotensive pregnant women are compared. Design: Case control study. Settings: Obstetricsand gynecology department of Jinnah hospital Lahore. Period: 1st October 2011 to 24 February2012. Subjects and methods: The case control study of 250 cases (pregnant females withhypertensive disorders) and controls (pregnant females without hypertensive disorder),presented at obstetrics & gynecology department of Jinnah hospital during 1st October 2011 to24 February 2012 was conducted. SPSS software (16) and MS excel were used for statisticalanalysis. Results: Mean age for cases and controls was 26.96 ± 5.29yearsand 25.25 ±4.60years, respectively. Age and history of pregnancy was found to be significantly associatedwith hypertensive disorders of pregnancy. Comparison of neonatal outcome between casegroup and control group showed that hypertensive pregnant women were at higher risk of havingadverse pregnancy outcome. Conclusions: Women with hypertension during pregnancy are atincreased risk of having adverse pregnancy outcome as compared to normotensive women andage, history of pregnancy induced hypertension are contributing risk factors for developinghypertension during pregnancy.

scholarly journals Seroprevalence of Subclinical HEV Infection in Healthy Pregnant Urban Dwellers of Bangladesh: Identification of Possible Risk Factors

2018 ◽  
Vol 8 (2) ◽  
pp. 85-89
Author(s):  
Gazi Zakia Sultana ◽  
Md Moniruzzaman ◽  
Tania Mannan ◽  
Rosy Sultana
Keyword(s):  
Risk Factors ◽  
Socioeconomic Status ◽  
Pregnant Women ◽  
Low Socioeconomic Status ◽  
Economic Status ◽  
Education Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Cross Sectional ◽  
History Of

Background: Hepatitis caused by hepatitis E Virus (HEV) is not uncommon in developing countries. It is usually a self-limiting conferring immunity against subsequent infection. However, HEV infection during pregnancy results in varying degree of morbidity, often fatal. The present study was designed to find out the seroprevalence of subclinical HEV infection during pregnancy at different trimesters without history of hepatitis.Materials and Methods: A total 255 asymptomatic healthy pregnant women of three trimesters (85×3=255) with no history of jaundice were included in this cross-sectional study. The subjects were sub-grouped according to socioeconomic status and education level. HEV IgG antibody in serum was determined by enzyme linked immunosorbent assay (ELISA). Results were expressed as number (percent). Chi-square, Odds Ratio and 95% CI were calculated as applicable. Data analyses were carried out using statistical package for social science for Windows Version 15.0. A p<0.05 was taken as level of significance.Results: Seropositivity for HEV IgG was 38% (96/255) in pregnant women; the higher percentages were recorded in the 2nd and 3rd trimesters − 41% and 46% respectively. The seropositivity of HEV IgG was significantly high in pregnant women with low education level ((p=0.001; OR=2.70, 95% CI=1.602−4.575) and low socioeconomic status (OR=7.54, 95% CI=4.118−13.029) having monthly income below 27,000 taka (p=0.001).Conclusion: Data concluded that seroprevalence of anti-HEV IgG is higher at third trimester in pregnant women in Bangladesh where low socio-economic status and less education level were identified as possible risk factors. Appropriate measures may diminish the possible exposure to infection and reduce maternal mortality.J Enam Med Col 2018; 8(2): 85-89

Seroprevalence of Neospora caninum in cats from the Czech Republic

2014 ◽  
Vol 59 (2) ◽  
Author(s):  
Kamil Sedlák ◽  
Eva Bartova ◽  
Tereza Machacova
Keyword(s):  
Czech Republic ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Cats ◽  
The Czech Republic ◽  
Indirect Fluorescence Antibody ◽  
Different Parts ◽  
Sera Samples

AbstractSera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years 2002–2011. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.

Sero-epidemiology of human T-cell lymphotropic viruses-1 and -2 infection among pregnant women attending Abuja Teaching Hospital, Nigeria

2021 ◽  
pp. 1-8
Author(s):  
Amos Dangana ◽  
Idris Nasir Abdullahi ◽  
Olasoji Matthew Adeyemi Billyrose ◽  
Anthony Uchenna Emeribe ◽  
Joel Monday Abu ◽  
...  
Keyword(s):  
Risk Factors ◽  
Pregnant Women ◽  
Teaching Hospital ◽  
Hiv Infections ◽  
Demographic Variables ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multiple Sexual Partners ◽  
Human T Cell ◽  
Increased Risk ◽  
History Of

BACKGROUND: There is the paucity of HTLV-1/-2 studies on Nigerian pregnant women despite the medical and public health significance of maternal-to-child transmission of HTLV-1/-2. OBJECTIVE: This study aims to determine the seroprevalence and risk factors of HTLV-1/-2 infections among pregnant women attending the University of Abuja Teaching Hospital (UATH), Abuja, Nigeria. MATERIALS AND METHODS: Blood samples were collected from consented pregnant women and analysed for ant-HTLV-1/-2 total antibodies using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) kit. Pretested structured questionnaires were used to collate participants’ socio-demographic variables and risk factors of HTLV infection. RESULTS: Out of the 156 pregnant women tested for HTLV-1/-2 antibodies, 16 (10.3%) were seropositive. There was no significant association between the socio-demographic variables collated and seroprevalence of HTLV-1/-2 infection among pregnant women (p> 0.05). Pregnant women with HIV infection had a lower prevalence of HLTV-1/-2 infection than those without HIV infections (7.5% versus 11.7%). Pregnant women with multiple sexual partners had a higher risk of HTLV-1/-2 infection than those who had single (OR = 2.08, 95% CI: 0.53–8.18). Women with a history of needles injury had a higher risk of HTLV-1/-2 infection than those who do not (OR = 1.24, 95% CI: 0.38–4.08). The history of blood transfusion was significantly associated with HTLV-1/-2 infection (p= 0.027). However, no significant association existed between other risk factors of HTLV-1/-2 infection among pregnant women (p> 0.05). CONCLUSION: Considering the 3% pooled national prevalence of HTLV-1/-2 infection in Nigeria, the seroprevalence reported in this study is relatively high. Thus, there is a need for more large cohort studies and routine screening of population at increased risk of infection.

scholarly journals Hepatitis B virus infection and its determinants among HIV positive pregnant women: Multicenter unmatched case-control study

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0251084
Author(s):  
Zelalem Alamrew Anteneh ◽  
Estifanose Wondaye ◽  
Endalkachew Worku Mengesha
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Pregnant Women ◽  
Case Control Study ◽  
Hbv Infection ◽  
Hiv Positive ◽  
Unsafe Sex ◽  
B Virus ◽  
History Of ◽  
Control Study

Background Hepatitis B virus (HBV) kills millions of people globally; it is worse in pregnant women. HBV and Human Immune Virus (HIV) co-infection is associated with increased liver diseases such as cirrhosis and hepatocellular carcinoma. This study aimed at identifying the determinants of HBV infection among HIV-positive pregnant women. Methods A multicentre unmatched case-control study was conducted among 109 cases (HBV/HIV co-infected) and 327 controls (HIV positive) pregnant women in seven hospitals of the Eastern Amhara region. Interview and chart review data collection techniques were employed by trained personnel. A binary logistic regression model was used to identify independent predictors of hepatitis B virus infection. Variables with a p-value of <0.05 and 95% confidence interval for odds ratio not containing 1 considered independent predictors of HBV infection. Results The findings of this study revealed that history of STI [AOR, 1.97, 95%CI, 1.09–3.56], hospital admission [AOR, 3.08, 95%CI, 1.69–5.61], traditional delivery care [AOR, 3.31, 95%CI, 1.72–6.37], family history of HBV [AOR, 3.33, 95%CI, 1.72–6.37], presence of opportunistic infections [AOR, 0.23, 95%CI, 0.12–0.58], viral load [AOR, 7.58, 95%CI, 3.18–8.01], CD4 count [AOR, 2.15, 95% CI, 1.01–4.59], anaemia [AOR, 3.07, 95% CI, 1.71–5.51] and unsafe sex [AOR, 1.98, 95%CI, 1.09–3.61] had a statistically significant association with HBV infection. Conclusions Several exposure variables had statistically significant association with HBV infection. High Viral Load appeared to be the largest predictor of HBV infection in HIV patients. Therefore, targeted interventions such as behavioral change intervention for unsafe sex and STI should be in place, and screening tests and treatment at the early stage of conception for both partners is necessary.

scholarly journals Hepcidin Status among Iron Deficient Anemic Pregnant Women in Gaza strip: A Case Control Study

2021 ◽  
Vol 29 (1) ◽  
Keyword(s):  
Pregnant Women ◽  
Case Control Study ◽  
Gaza Strip ◽  
Transferrin Saturation ◽  
Case Control ◽  
Deficiency Anemia ◽  
Enzyme Linked Immunosorbent Assay ◽  
Iron Deficient ◽  
Serum Hepcidin ◽  
Control Study

Background: Hepcidin, a peptide hormone composed of 25 amino acids. Hepcidin is synthesized mainly in the liver. Iron deficiency anemia (IDA) is common during pregnancy and is associated with higher maternal morbidity and mortality in Gaza strip. Understanding of hepcidin hormone and its role in iron metabolism could lead to a new sensitive indicator for earlier detection of cases with IDA. Objective: To assess hepcidin status among IDA pregnant women and its relationship with some biochemical variables in Gaza strip. Materials and methods: A case control study comprised 45 IDA pregnant women and 45 apparently healthy pregnant women. Questionnaire interviews were applied among the study population. Serum hepcidin and ferritin were measured by Enzyme-linked immunosorbent assay (ELISA), iron and total iron binding capacity (TIBC) were determined photometrically. Complete blood count (CBC) was also performed. Transferrin and transferrin saturation were calculated. An approval was obtained from Helsinki committee and ministry of health to conduct this study. Overall data were computer analyzed using SPSS (Ver. 18). Results: The mean levels of serum hepcidin, iron, transferrin saturation, and ferritin in cases were significantly lower compared to those of controls (2.6±4 ng/ml, 63.2±25.3 µg/dl, 15.6±8.0% and 8.0±9.7 ng/ml versus 7.5±7.3 ng/ml, 77.7±22.9 µg/dl, 23.5±8.0% and 15.4±14.3 ng/ml respectively with P=0.000). The Pearson correlation test showed that positive significant correlations between hepcidin levels and serum iron, ferritin, and transferrin saturation (P<0.001). On the other hand, negative significant correlations were showed with TIBC and transferrin (P<0.001). Conclusions: Serum hepcidin level has a relationship with anemia among pregnant women. Therefore, monitoring of hepcidin levels can play an important role in management of anemia among pregnant women.

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