scholarly journals RANKL EXPRESSION AND METABOLIC CHANGES IN OVARIECTOMIZED RATS AND THE POSSIBLE PROTECTION BY VEGETABLE FORMULA.

2018 ◽  
Vol 11 (1) ◽  
pp. 318
Author(s):  
Emtenan M Hanafi
Keyword(s):  
High Performance ◽  
Mass Density ◽  
Reducing Power ◽  
Dry Weight ◽  
Ovariectomized Rats ◽  
Bone Mass Density ◽  
Control Group ◽  
Oxidative Markers ◽  
Gapdh Mrna ◽  
Lower Blood

 Objective: This study was to evaluate complications of osteoporosis in ovariectomized rats and the possibility to ameliorate these changes by consumption of vegetable formula. Furthermore, transcription of mRNA of RANKL gene was matched with bone mass density (BMD) and bone formation marker (human procollagen 1 N terminal peptide [PINP]).Methods: Thirty rats were divided into three groups. The first is non ovariectomized control group (NOVXC), the second is ovariectomized control group (OVXC), and the third is ovariectomized rats supplemented with the vegetable formula (OVXT). Animals were fed for 8 successive weeks. Animals were treated and sacrificed under the recommended ethics of laboratory animal’s treatment. The vegetable mixture was formulated with the purpose to correct the bone compromise and supply all the presumed deficient elements and hormone.Results: Chemical analysis showed that the formulated vegetable mixture had a high amount of flavonoids as catechin (100 mg/100 g of dry weight) and polyphenols as tannic acid (1000 mg/100 g dry weight. Furthermore, it had high reducing power (1,1-diphenyl-2-picrylhydrazyl radical showed inhibition percentage of 91.81%.). Several phytochemicals necessary for bone health were demonstrated in the vegetable mixture using high-performance liquid chromatography. RANKL/GAPDH mRNA transcription ratio showed marked an increase in OVXC versus the control NOVXC rats (1.00 vs. 0.199, respectively) accompanied with a drop in BMD (0.157 vs. 0.25 mg/cm2, respectively) and PINP values (27.9±2.8 compared to NOVXC 34±2.4 μ/L, respectively). The vegetable mixture supplementation showed better values of BMD and PINP in OVXT group directed back toward normal (0.183 mg/cm2 and 29.35±3.4 μ/L, respectively). Furthermore, analysis of blood plasma of supplemented group showed lower blood glucose, lipid profile, and oxidative markers if compared to that in OVXC group.Conclusion: It may be concluded that the plant formula was effective to minimize health hazards in ovariectomized rats and maybe for postmenopause women. Perhaps longer time may be needed for more significant and clear effect.

scholarly journals RANKL EXPRESSION AND METABOLIC CHANGES IN OVARIECTOMIZED RATS AND THE POSSIBLE PROTECTION BY VEGETABLE FORMULA.

2018 ◽  
Vol 11 (1) ◽  
pp. 318
Author(s):  
Emtenan M Hanafi
Keyword(s):  
High Performance ◽  
Mass Density ◽  
Reducing Power ◽  
Dry Weight ◽  
Ovariectomized Rats ◽  
Bone Mass Density ◽  
Control Group ◽  
Oxidative Markers ◽  
Gapdh Mrna ◽  
Lower Blood

 Objective: This study was to evaluate complications of osteoporosis in ovariectomized rats and the possibility to ameliorate these changes by consumption of vegetable formula. Furthermore, transcription of mRNA of RANKL gene was matched with bone mass density (BMD) and bone formation marker (human procollagen 1 N terminal peptide [PINP]).Methods: Thirty rats were divided into three groups. The first is non ovariectomized control group (NOVXC), the second is ovariectomized control group (OVXC), and the third is ovariectomized rats supplemented with the vegetable formula (OVXT). Animals were fed for 8 successive weeks. Animals were treated and sacrificed under the recommended ethics of laboratory animal’s treatment. The vegetable mixture was formulated with the purpose to correct the bone compromise and supply all the presumed deficient elements and hormone.Results: Chemical analysis showed that the formulated vegetable mixture had a high amount of flavonoids as catechin (100 mg/100 g of dry weight) and polyphenols as tannic acid (1000 mg/100 g dry weight. Furthermore, it had high reducing power (1,1-diphenyl-2-picrylhydrazyl radical showed inhibition percentage of 91.81%.). Several phytochemicals necessary for bone health were demonstrated in the vegetable mixture using high-performance liquid chromatography. RANKL/GAPDH mRNA transcription ratio showed marked an increase in OVXC versus the control NOVXC rats (1.00 vs. 0.199, respectively) accompanied with a drop in BMD (0.157 vs. 0.25 mg/cm2, respectively) and PINP values (27.9±2.8 compared to NOVXC 34±2.4 μ/L, respectively). The vegetable mixture supplementation showed better values of BMD and PINP in OVXT group directed back toward normal (0.183 mg/cm2 and 29.35±3.4 μ/L, respectively). Furthermore, analysis of blood plasma of supplemented group showed lower blood glucose, lipid profile, and oxidative markers if compared to that in OVXC group.Conclusion: It may be concluded that the plant formula was effective to minimize health hazards in ovariectomized rats and maybe for postmenopause women. Perhaps longer time may be needed for more significant and clear effect.

scholarly journals Swim-trained rats have greater bone mass, density, strength, and dynamics

2001 ◽  
Vol 91 (4) ◽  
pp. 1663-1668 ◽  
Author(s):  
K. J. Hart ◽  
J. M. Shaw ◽  
E. Vajda ◽  
M. Hegsted ◽  
S. C. Miller
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Bone Structure ◽  
Mass Density ◽  
Weight Bearing ◽  
Femoral Shaft ◽  
Ovariectomized Rats ◽  
Bone Mass Density ◽  
Control Group ◽  
Mineral Density

Weight-bearing exercise is traditionally recommended for improving bone health in postmenopausal women. Effects of swim exercise were studied as an alternative to weight-bearing exercise in ovariectomized rats. Rats in a swim group (Sw, n = 8) swam for 12 wk, 5 days/wk for 60 min per session. A control group (Con, n = 9) engaged in no structured exercise. Femurs were analyzed for bone mineral density and for bone mineral content by dual energy X-ray absorptiometry, biomechanical properties by three-point bending (Instron), and bone structure and formation by histomorphometry. Food intake did not differ among groups. Final body weights were significantly lower in Sw compared with Con ( P< 0.05). Swimmers had significantly greater femoral shaft bone mineral density and content ( P < 0.05) compared with Con. Femurs of the Sw group had greater mechanical properties ( P< 0.05) compared with Con. Histomorphometric data were significantly better in the Sw group compared with Con after the 12-wk intervention ( P < 0.05). In conclusion, data from this study demonstrate some beneficial effects of swim exercise on bone structure, turnover, and strength.

scholarly journals The effect of PDE5 inhibitors on bone and oxidative damage in ovariectomy-induced osteoporosis

2017 ◽  
Vol 242 (10) ◽  
pp. 1051-1061 ◽  
Author(s):  
Hamit H Alp ◽  
Zübeyir Huyut ◽  
Serkan Yildirim ◽  
Yıldıray Başbugan ◽  
Levent Ediz ◽  
...  
Keyword(s):  
Mass Density ◽  
Public Health Problem ◽  
Cyclic Guanosine Monophosphate ◽  
Guanosine Monophosphate ◽  
Protein Kinase G ◽  
Bone Mass Density ◽  
Control Group ◽  
Major Public Health Problem ◽  
Pde5 Inhibitors ◽  
Positive Effects

Osteoporosis is a major public health problem associated with many factors, and it affects more than 50% of women over 50 years old. In the current study, our purpose was to investigate the effects of phosphodiestarase-5 inhibitors on osteoporosis via the nitric oxide/3′,5′-cyclic guanosine monophosphate/protein kinase G signalling pathway. A total of 50 female albino Wistar rats were separated into five groups. The first group was appointed as the healthy control group with no ovariectomy. All animals in the other groups underwent a bilateral ovariectomy. Six months after the ovariectomy, vardenafil, udenafil and tadalafil were given to the third, fourth and fifth groups, respectively, but were not administered to the positive control group (10 mg/kg per day for two months). The bone mineral density values were determined using a densitometry apparatus for all groups pre- and post-ovariectomy as well as after treatment. The levels of nitric oxide, endothelial nitric oxidesynthase, asymmetric dimethylarginine, 3′,5′-cyclic guanosine monophosphate, protein kinase G, phosphodiestarase-5, pyridinoline, deoxypyridinoline, carboxyterminal telopeptide fragments and plasma carboxy terminal propeptide of type I collagen were determined using an enzyme linked immunosorbent assay. The levels of malondialdehyde, 8-hydroxy-2-deoxy guanosine, deoxyguanosine and coenzyme Q10 were determined by a high-performance liquid chromatography assay. Additionally, the right femoral trabecular bone density and the epiphyseal plate were measured in all groups. Angiogenesis was histologically observed in the bone tissue. In addition, we determined that the inhibitors may have caused a positive impact on the increased bone mass density and reduction of bone resorption markers. We also observed the positive effects of these inhibitors on oxidative stress. In conclusion, these phosphodiestarase-5 inhibitors increase angiogenesis in bone tissue and improve the re-formation rate of bone in rats with osteoporosis. Chemical compounds studied in this article Udenafil (PubChem CID: 6918523); Tadalafil (PubChem CID: 110635); Vardanafil (PubCham CID: 110634). Impact statement The results in our study appear to establish the osteoporosis model and provide evidence of the positive effects of three separate PDE5 inhibitors (vardenafil, udenafil, and tadalafil). The positive effects of these PDE5 inhibitors are investigated and demonstrated by the bone mass density and bone resorption markers. These effects are associated with significant demonstrated antioxidant activities. Osteoporosis is a significant major public health problem especially in more aged populations. Advances in identifying and understanding new potential therapeutic modalities for this disease are significant. This study provides such an advance.

scholarly journals Ophiorrhiza mungos var. angustifolia – Estimation of camptothecin and pharmacological screening

2018 ◽  
Vol 5 (3) ◽  
pp. 113-120 ◽  
Author(s):  
G Krishna Kumar ◽  
A Muhammed Fayad ◽  
A Jayakumaran Nair
Keyword(s):  
High Performance ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Therapeutic Principles ◽  
Reducing Power Assay

Ophiorrhiza mungose var. angustifolia (Thwaites) Hook. f (Family- Rubiaceae) is a recently identified plant from Ophiorrhiza species in Western Ghats of Kerala. The plant is a promising candidate for the production of camptothecin (CPT) - a high value anticancer compound. Preliminary screening of hexane and methanol extract revealed the presence of phenolics, flavonoids, caumarins, steroids, terpeanoids, saponins, carbohydrates and alkaloids. Camptothecin was estimated from methanol extract using high performance liquid chromatography and the level of CPT was 297.94 ± 2.27 µg/g dry weight. The in vitro antioxidant assay revealed both extract showed moderate level of total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferric chloride reducing power assay, phospho-molybdate assay of total anti-oxidant capacity and nitric oxide scavenging activity assay. Antimicrobial study reveals that only hexane extract inhibits pathogenic bacteria and fungus. Overall these findings will lead to isolation of active compounds other than camptothecin, elucidate them against wider range of bioactivity studies to find new therapeutic principles.

scholarly journals Assessment bone mass density in new diagnosed Grave’s disease

2021 ◽  
pp. 11-17
Author(s):  
Silathod BoutsaBa ◽  
Trung Quan Do
Keyword(s):  
Bone Mass ◽  
Mass Density ◽  
Bone Mass Density ◽  
Control Group ◽  
Thigh Bone ◽  
T Score ◽  
Grave’S Disease ◽  
Normal People ◽  
Study Results ◽  
Grave's Disease

Object: To assess bone mass density in new diagnosed Grave’s disease. Subjects: 60 Grave’s disease patients and 50 normal people that visited at outpatient department for required service, Bach Mai hospital, from October 2017 to July 2018. Methods: Cross-sectional study. Results: 6,66% of patients had osteoporosis at neck of thigh bone and this rate was 3,33% at spine. The bone mass density in new diagnosed Grave’s disease was lower than normal people. At neck of thigh bone: bone mass density in Grave’s disease was - 0,89 ± 1,07 (T - score) but only - 0,58 ± 1,06 (T - score) in control group (p > 0,05). At spine: bone mass density in Grave’s disease was - 0,35 ± 1,22 (T - score) and in control group was 0,068 ± 1,06 (T - score) (p < 0,05). Conclusions: There was no correlation between thyroid volume and bone mass density.

scholarly journals Is Walking Exercise a Protective Factor for Osteoporosis in Young Obese Women?

2010 ◽  
Vol 25 (1) ◽  
pp. 41-47
Author(s):  
Nasim Habibzadeh ◽  
Farhad Rahmani-Nia ◽  
Hassan Daneshmandi
Keyword(s):  
Protective Factor ◽  
Mass Density ◽  
Exercise Group ◽  
Blood Analysis ◽  
Bone Mass Density ◽  
Control Group ◽  
Maximal Heart Rate ◽  
Obese Women ◽  
Related Factors ◽  
Walking Exercise

Is Walking Exercise a Protective Factor for Osteoporosis in Young Obese Women?Twenty obese (BMI>30) women with an average age of 22.00 ± 1.50 years volunteered to participate in this study. They were randomly assigned to exercise (n=10) and control (n=10) groups. Before and after the training program, both groups were evaluated for anthropometric measurements and blood analysis. Bone mass density (BMD) was evaluated by using dual-energy X-ray absorptiometry (DXA) at the hip and spine (L2-L4). Each walking session was 30 min walking at 50-75% of maximal heart rate, 3 days per week, for 2 months. After 2 months, the exercise group showed no significant effect on BMD at the spine (L2-L4) and hip (p>0.05). However, there was a slightly increase in BMD at both regions, while the control group did not. Also, no change was observed in bloods factors. Percent body fat, fat mass and lean mass changes in response to training were significant in the exercise group (all p=0.000). As a result, it may be suggested that walking programs of longer duration and higher intensity can influence BMD and related factors in obese girls, however to achieve this result, more studies are needed.

scholarly journals The Influence of Statins on Bone Tissue in Ovariectomized Rats

2018 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Bone Density ◽  
Mass Density ◽  
Dual Energy ◽  
Bone Architecture ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Bone Mass Density ◽  
X Ray ◽  
Skeletal Disease ◽  
Coa Reductase

Objectives: Osteoporosis is a silent, progressive, systemic skeletal disease characterized by impaired bone architecture with decreased bone mass density, affecting mainly women. Statins, inhibitors of HMG-CoA-reductase, seem to interfere with bone formation exerting anabolic and anti-resorptive effect. The aim of the present study is to investigate the effect of simvastatin on size parameters and bone density of mandible and femur, in an ovariectomized rat model of osteoporosis. Methods: 50 Wistar female rats were randomized in five groups (n=10). Groups 1, 2,3 were ovariectomized, 4 and 5 serve as controls. Groups 2, 3 and 5 were treated with simvastatin (0.5mg/kg/daily per os). Size parameters of the isolated femur, mandible and uterus, BMD via dual energy X-Ray absorptiometry (DEXA) and serum interleukin-13 (IL-13) were estimated. Results: DEXA results showed significant decrease of BMD in ovariectomized group / controls while statins treatment decreased the severity of BMD lose. The serum IL-13, as osteogenesis index, seems to be positively influenced by statins treatment. Conclusions: Statins treatment seems to restore the decreased femur and mandible parameters and uterus weight in experimental menopause following ovariectomy.

scholarly journals Wnt-Signaling-Mediated Antiosteoporotic Activity of Porcine Placenta Hydrolysates in Ovariectomized Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Byoung-Seob Ko ◽  
Da Sol Kim ◽  
Suna Kang ◽  
Na Ra Lee ◽  
Jin Ah Ryuk ◽  
...  
Keyword(s):  
Amino Acids ◽  
Wnt Signaling ◽  
Bone Turnover Marker ◽  
Mass Density ◽  
Ovarian Hormones ◽  
Ovariectomized Rats ◽  
Bone Mass Density ◽  
Related Protein ◽  
Bone Specific Alkaline Phosphatase ◽  
Ovx Rats

Anti-osteoporotic effects of two types of porcine placenta hydrolysates (PPH) were evaluated in ovariectomized (OVX) rats orally administered PPH without (WPPH) or with (NPPH) ovarian hormones (1 g/kg bw/day). PPH groups were compared with OVX rats with estrogen replacement (0.1 mg/kg bw conjugated estrogen; EST), or dextrose (placebo; OVX-control) All rats received high-fat/calcium-deficient diets for 12 weeks. NPPH contained less estrogen and progesterone, but more essential amino acids, whereas the opposite was true for WPPH. NPPH decreased body weight and peri-uterine fat pads, and maintained uterus weight. NPPH rats had higher femur and lumbar spine bone mass density compared to controls; but less than those of EST rats. Serum phosphorus and urinary calcium and phosphorus levels were reduced in NPPH rats compared to OVX-controls. Serum bone-specific alkaline phosphatase, osteocalcin, and bone turnover marker levels were reduced NPPH rats compared to OVX-controls. WPPH produced results similar to those of NPPH, but less significant. Both NPPH and estrogen upregulated low-density lipoprotein receptor-related protein 5 andβ-catenin in OVX rats, while the expression of dickkopf-related protein 1 was suppressed. In conclusion, NPPH exerted anti-osteoporotic effects by activating osteogenesis and stimulating Wnt signaling, possibly mediated by the various amino acids and not ovarian hormones.

scholarly journals Broccoli (Brassica oleracea L. var. italica) Sprouts as the Potential Food Source for Bioactive Properties: A Comprehensive Study on In Vitro Disease Models

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 532 ◽  
Author(s):  
Thanh Ninh Le ◽  
Hong Quang Luong ◽  
Hsin-Ping Li ◽  
Chiu-Hsia Chiu ◽  
Pao-Chuan Hsieh
Keyword(s):  
High Performance ◽  
Food Source ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Dry Weight ◽  
Carcinoma Cells ◽  
Broccoli Sprouts ◽  
Potential Food Source ◽  
Potential Food

Broccoli sprouts are an excellent source of health-promoting phytochemicals such as vitamins, glucosinolates, and phenolics. The study aimed to investigate in vitro antioxidant, antiproliferative, apoptotic, and antibacterial activities of broccoli sprouts. Five-day-old sprouts extracted by 70% ethanol showed significant antioxidant activities, analyzed to be 68.8 μmol Trolox equivalent (TE)/g dry weight by 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic (ABTS) assay, 91% scavenging by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 1.81 absorbance by reducing power assay, and high phenolic contents by high-performance liquid chromatography (HPLC). Thereafter, sprout extract indicated considerable antiproliferative activities towards A549 (lung carcinoma cells), HepG2 (hepatocellular carcinoma cells), and Caco-2 (colorectal adenocarcinoma cells) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, with IC50 values of 0.117, 0.168 and 0.189 mg/mL for 48 h, respectively. Furthermore, flow cytometry confirmed that Caco-2 cells underwent apoptosis by an increase of cell percentage in subG1 phase to 31.3%, and a loss of mitochondrial membrane potential to 19.3% after 48 h of treatment. Afterward, the extract exhibited notable antibacterial capacities against Bacillus subtilis and Salmonella Typhimurium with minimum inhibition concentration (MIC) values of 0.39 and 0.78 mg/mL, appropriately, along with abilities against Staphylococcus aureus and Escherichia coli with an MIC value of 1.56 mg/mL. Thus, broccoli sprouts were confirmed as a potential food source for consumers’ selection and functional food industry.

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