ASSOCIATION OF INTERLEUKIN-4 CYTOKINE AND IL-4Rα GENE POLYMORPHISM IN β-LACTAM ALLERGIC PATIENTS

Objective: The present study was carried out to estimate the possible role of Interleukin-4 (IL-4)RαQ576R genes polymorphism in the development of immune reaction against penicillin, as well as to study the effect of IL-4 cytokine in regulating allergic reactions.Materials and Methods: Measurement of serum IL-4 concentration was done using enzyme-linked immunosorbent assay technique; IL-4RαQ576R gene polymorphisms were genotyped using polymerase chain reaction-restriction fragment lengths polymorphisms. Comparisons for statistical significance were performed using Mann–Whitney U-test.Results: Comparing with control subjects, there was a significantly increased level of IL-4 (348.53 pg/ml) in penicillin allergic patients versus (284.72 pg/ml) in sera of control subjects. The IL-4RαQ576R alleles were significantly higher in the penicillin allergic individual compared with apparently healthy control subjects.Conclusions: Data study suggested that IL-4 cytokine have some important roles in penicillin hypersensitivity reaction, additionally the IL- 4RαQ576Rgene polymorphisms might involve in modulating of penicillin hypersensitivity.

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Study of the Immunological Status of Iraqi Vitiligo Patients

Baghdad Science Journal ◽

10.21123/bsj.13.3.454-462 ◽

2016 ◽

Vol 13 (3) ◽

pp. 454-462

Author(s):

Baghdad Science Journal

Keyword(s):

Potential Role ◽

Immunoglobulin E ◽

Ultraviolet B ◽

Enzyme Linked Immunosorbent Assay ◽

Control Serum ◽

Healthy Control ◽

The Mean ◽

Age Range ◽

Apparently Healthy

Vitiligo is an acquired idiopathic skin disorder characterized by depigmented macules due to loss of cutaneous melanocytes. A potential role of the immune dysfunction has been suggested in vitiligo, so to test this hypothesis, certain cytokines (IL-17A and TNF-?) and immunoglobulins (IgM, IgG, IgA and total IgE) were investigated in all participants. The study included: 60 patients with age range between (6-55) year; 30(11 males and 19 females) were untreated and 30(12 males and 18 females) were treated with Narrow Band Ultraviolet-B (NB-UVB) and 30 (14 males and 16 females) apparently healthy control. Serum was separated and cytokines (IL-17A and TNF-?) and total immunoglobulin E (IgE) were detected by using Enzyme Linked Immunosorbent Assay (ELISA); while immunoglobulins (IgM, IgG and IgA) were detected by using Single Radial Immunodiffusion (SRID) method. The results showed that the mean levels of serum IL-17A and TNF-? in both untreated and NB-UVB treated vitiligo patients were increased significantly (p ? 0.05) as compared with healthy control. The mean levels of serum IgG and IgA in untreated vitiligo patients showed non significant decreased (P

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Association between AUTS2 haplotypes and alcohol dependence in a Japanese population

Acta Neuropsychiatrica ◽

10.1017/neu.2015.70 ◽

2016 ◽

Vol 28 (4) ◽

pp. 214-220 ◽

Cited By ~ 2

Author(s):

Shin Narita ◽

Kenta Nagahori ◽

Daisuke Nishizawa ◽

Eiji Yoshihara ◽

Atsuko Kawai ◽

...

Keyword(s):

Alcohol Dependence ◽

Fragment Length Polymorphism ◽

Allele Frequencies ◽

Chain Reaction ◽

Control Subjects ◽

Genome Wide ◽

Healthy Control ◽

Polymerase Chain ◽

And Control ◽

Genotype And Allele Frequencies

ObjectiveRecent genome-wide analysis has indicated that the autism susceptibility candidate 2 (AUTS2) gene is involved in the regulation of alcohol consumption. We hypothesised that AUTS2 might be associated with the development of alcohol dependence. Therefore, in this exploratory study, we compared the genotype and allele frequencies of the polymorphisms rs6943555 and rs9886351 in the AUTS2 gene between patients with alcohol dependence and healthy control subjects living in a Japanese provincial prefecture. We also examined whether or not the haplotypes consisting of these polymorphisms are related to alcohol dependence.MethodsThe subjects of this study consisted of 64 patients with alcohol dependence and 75 unrelated healthy people. The AUTS2 genotypes were determined by the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method.ResultsNo significant differences in the genotype and allele frequencies of the polymorphisms AUTS2 rs6943555 and rs9886351 were found between alcohol dependence and control subjects. On the other hand, the frequencies of the AUTS2 haplotypes were significantly different between them, and the rs6943555 and rs9886351 A-A haplotype was associated with alcohol dependence (p=0.0187).ConclusionThis suggests that the rs6943555 and rs9886351 A-A haplotype might affect the vulnerability to alcohol dependence pathogenesis. Further studies are needed to confirm the reproducibility of the results of this study with increased numbers of subjects.

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Association of Bartonella species, feline calicivirus, and feline herpesvirus 1 infection with gingivostomatitis in cats

Journal of Feline Medicine and Surgery ◽

10.1016/j.jfms.2009.10.007 ◽

2010 ◽

Vol 12 (4) ◽

pp. 314-321 ◽

Cited By ~ 48

Author(s):

Kristy L. Dowers ◽

Jennifer R. Hawley ◽

Melissa M. Brewer ◽

Arianne K. Morris ◽

Steven V. Radecki ◽

...

Keyword(s):

Conventional Polymerase Chain Reaction ◽

Enzyme Linked Immunosorbent Assay ◽

Feline Calicivirus ◽

Healthy Controls ◽

Bartonella Species ◽

Chain Reaction ◽

Feline Herpesvirus ◽

Healthy Control ◽

Herpesvirus 1 ◽

Polymerase Chain

Feline gingivostomatitis (FGS) is a common syndrome in cats; feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and Bartonella species are common differential diagnoses. In this study, blood from 70 cats with FGS and 61 healthy control cats was tested for Bartonella species antibodies in serum by enzyme-linked immunosorbent assay and Western blot immunoassay and DNA in blood using a conventional polymerase chain reaction assay. Additionally, fresh oral biopsies from cats with FGS ( n=42) and 19 healthy controls were tested for FCV RNA, FHV-1 DNA and Bartonella species DNA. The prevalence rates for Bartonella species antibodies and DNA in the blood and the tissues did not differ between the two groups. FHV-1 DNA was also not significantly different between groups. Only FCV RNA was present in significantly more cats with FGS (40.5%) than control cats (0%). The results suggest that FCV was associated with FGS in some of the cats.

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Association of IL-4RαQ576R gene polymorphisms and IL-4,IFN-γ cutokines and total IgE levels in β-Lactam allergic patients.

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1363 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Dhuha Ali Hasan ◽

Manal M Al-Saaidy

Keyword(s):

Crucial Role ◽

Gene Polymorphisms ◽

Immune Reaction ◽

Allergic Reactions ◽

Total Ige ◽

Lactam Antibiotic ◽

Healthy Control ◽

Ifn Γ ◽

Apparently Healthy

Penicillin considered the most familiar β-lactam antibiotic that causing allergic reactions with an incidence rate of 0.7-10% of all population. Excessive release of IL-4and IFN-γ is thought to be important in development and regulation of atopic and other allergic disorders.To estimate the possible role of IL-4RαQ576Rgenes polymorphism in development of immune reaction against penicillin,and study the effect of IL-4 and IFN-γ in regulating of allergic reactions.Measurement ofserum levels of IL-4,IFN-γ and total IgE done by using enzyme linkedd immunosorbentt assaya (ELISAa) technique. IL-4RαQ576Rgene polymorphisms were genotyped by using polymerasee chainn reactions restrictions fragments lengths polymorphisms (PCRr-RFLPp).Comparing with ccontrol subjectss,there was a significant higher levels of IL-4,IFN-γ and IgE in patients group,they were (348.53 pg/ml) versus (284.72pg/ml) and (194.33pg/ml) versus (159.82pg/ml) and (357.28pg/ml) versus (124.88pg/ml) respectively with P=0.001. The IL-4RαQ576Ralleles were significantly higher in penicillin allergic individual compared with apparently healthy control subjects. Results suggested that IL-4and IFN-γ cytokines play a crucial role in modulating of B-lactam allergy. IL-4RαQ576Rgene polymorphismsmight involvein development of penicillin hypersensitivity.

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METTL3 Attenuates LPS-Induced Inflammatory Response in Macrophages via NF-κB Signaling Pathway

Mediators of Inflammation ◽

10.1155/2019/3120391 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Jinghua Wang ◽

Shushan Yan ◽

Hongying Lu ◽

Shufeng Wang ◽

Donghua Xu

Keyword(s):

Inflammatory Response ◽

Critical Role ◽

Underlying Mechanism ◽

Enzyme Linked Immunosorbent Assay ◽

Macrophage Cell Line ◽

Macrophage Cell ◽

Chain Reaction ◽

Key Enzyme ◽

Polymerase Chain

Methyltransferase-like 3 (METTL3), an RNA N6-methyladenosine (m6A) methyltransferase, is essential for the m6A mRNA modification. As a key enzyme of m6A methylation modification, METTL3 has been implicated in immune and inflammation regulation. However, little is known of the role and underlying mechanism of METTL3 in rheumatoid arthritis (RA). The aim of the present study is to elucidate the function and potential mechanism of METTL3 in RA pathogenesis. We used quantitative real-time polymerase chain reaction to detect the expression of METTL3 in RA patients and controls as well as the macrophage cell line. CCK-8 was used for cell proliferation assay. Enzyme-linked immunosorbent assay (ELISA) was adopted to estimate the generation of IL-6 and TNF-α in macrophages. Western blot and immunofluorescence were applied to evaluate the activation of NF-κB in macrophages. The expression of METTL3 was significantly elevated in patients with RA. It was positively associated with CRP and ESR, two common markers for RA disease activity. Besides, LPS could enhance the expression and biological activity of METTL3 in macrophages, while overexpression of METTL3 significantly attenuated the inflammatory response induced by LPS in macrophages. Moreover, the effect of METTL3 on LPS-induced inflammation in macrophages was dependent on NF-κB. This study firstly demonstrates the critical role of METTL3 in RA, which provides novel insights into recognizing the pathogenesis of RA and a promising biomarker for RA.

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Helicobacter pylori Recombinant CagA Regulates Th1/Th2 Balance in a BALB/c Murine Model

Advanced Pharmaceutical Bulletin ◽

10.34172/apb.2020.031 ◽

2020 ◽

Vol 10 (2) ◽

pp. 264-270

Author(s):

Nafiseh Paydarnia ◽

Behzad Mansoori ◽

Davoud Esmaeili ◽

Tohid Kazemi ◽

Mahyar Aghapour ◽

...

Keyword(s):

Helicobacter Pylori ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Chain Reaction ◽

Protein Levels ◽

Cytotoxin Associated Gene A ◽

Polymerase Chain ◽

H Pylori ◽

Antibody Levels

Purpose: Helicobacter pylori is recognized as one of the prevalent causes of human gastricinfection. In the present study, the role of mixed immunization with H. pylori lipopolysaccharide(LPS) and recombinant cytotoxin-associated gene A (rCagA) as a stimulator of host immuneresponses was determined. Methods: BALB/c mice were immunized with different formulations by the systemic administrationat 14-day intervals. The effects of the formulations plus CpG adjuvants were assessed before andpost-immunization in separated studies. Moreover, the expression of Th1/Th2 cytokines wasquantified in sera of immunized mice using reverse transcription polymerase chain reaction (RTPCR)test and the protein levels confirmed with enzyme linked immunosorbent assay (ELISA).Finally, the specific antibody levels in sera were studied by ELISA and the tendency of cellularresponse was examined by IgG1/IgG2a ratio. Results: Data of Western blotting verified the presence of constructed protein. Analysisof lymphocyte proliferation showed that CpG-conjugated rCagA increases lymphocytesproliferation compared to the control group. Also, it was shown that formulations containing LPSand rCagA promote a Th1 response indicated by interferon-gamma expression and induced Th1/Th2 balance. Additionally, the specific IgG1, total IgG and IgG2a levels elevated in response toall treatments. Ultimately, the IgG2a/IgG1 ratio in the mice immunized with rCagA-containingformulations increased. Conclusion: These results indicated that rCagA protein carried with CpG adjuvant not onlymaintained its antigenicity throughout the experiment but also induced robust Th1-biasedimmune responses. Therefore, it holds promise for the production of an efficient vaccine againstH. pylori infection.

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Higher expression of monocyte chemotactic protein 1 in mild COVID-19 patients might be correlated with inhibition of IFN signaling

10.21203/rs.3.rs-71401/v4 ◽

2020 ◽

Author(s):

Xueyan Xi ◽

Yang Guo ◽

Min Zhu ◽

Yuhui Wei ◽

Gang Li ◽

...

Keyword(s):

Chemokine Receptors ◽

Serum Levels ◽

Significant Negative Correlation ◽

Enzyme Linked Immunosorbent Assay ◽

Chain Reaction ◽

Monocyte Chemotactic Protein ◽

Chemotactic Protein ◽

Polymerase Chain

Abstract Background: Chemokine levels in severe coronavirus disease 2019 (COVID-19) patients have been shown to be markedly elevated. But the role of chemokines in mild COVID-19 has not yet been established. According to the epidemiological statistics, most of the COVID-19 cases in Shiyan City, China, have been mild. The purpose of this study was to evaluate the level of chemokines in mild COVID-19 patients and explore the correlation between chemokines and host immune response.Methods: In this study, we used an enzyme-linked immunosorbent assay (ELISA) to detect serum levels of chemokines in COVID-19 patients in Shiyan City. Expression of chemokine receptors and of other signaling molecules was measured by real-time polymerase chain reaction (PCR).Results: We first demonstrated that COVID-19 patients, both sever and mild cases, are characterized by higher level of chemokines. Specifically, monocyte chemotactic protein 1 (MCP-1) is expressed at higher levels both in severe and mild cases of COVID-19. The receptor of MCP-1, C-C chemokine receptor type 2 (CCR2), was expressed at higher levels in mild COVID-19 patients. Finally, we observed a significant negative correlation between expression levels of interferon (IFN) regulatory factor 3 (IRF3) and serum levels of MCP-1 in mild COVID-19 patients.Conclusion: Higher expression of MCP-1 in mild COVID-19 patients might be correlated with inhibition of IFN signaling. The finding adds to our understanding of the immunopathological mechanisms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and provides potential therapeutic targets and strategies.

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The Role of Angiopoietin-2 Gene Mutation on Clinical Severity of Dengue Disease in Children

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.5288 ◽

2020 ◽

Vol 8 (B) ◽

pp. 1110-1115

Author(s):

Mayetti Akmal ◽

Amirah Zatil Izzah ◽

Jamsari ◽

Eriyati Darwin ◽

Dadang Hudaya Somasetia

Keyword(s):

Dengue Shock Syndrome ◽

Cross Sectional Study ◽

Clinical Severity ◽

Enzyme Linked Immunosorbent Assay ◽

Chain Reaction ◽

Cross Sectional ◽

Dengue Disease ◽

Polymerase Chain ◽

Angiopoietin 2

BACKGROUND: In general, angiopoietin-2 levels are increased concomitantly with dengue clinical severity. AIM: This research aims to determine the role of mutation on angiopoietin-2 on dengue clinical severity. METHODS: A cross-sectional study of 108 children with dengue disease grouped by severity. Angiopoietin-2 level was examined by enzyme-linked immunosorbent assay. Polymerase chain reaction and double nucleic acid sequencing are using 2 Exon 4-F primers. RESULTS: Angiopoietin-2 levels on rs7834131 mutant are higher in dengue fever (p < 0.05) and dengue hemorrhage fever group than non-mutant, while on dengue shock syndrome, it is lower than non-mutant. CONCLUSION: Angiopoietin-2 mutation on rs7834131 might have a protective effect on dengue disease severity.

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Evaluation of Sandwich Enzyme-Linked Immunosorbent Assay and Reverse Transcription Polymerase Chain Reaction for the Diagnosis of Foot-and-Mouth Disease

Intervirology ◽

10.1159/000517003 ◽

2021 ◽

pp. 1-6

Author(s):

Salman Khan ◽

Syed Asad Ali Shah ◽

Syed Muhammad Jamal

Keyword(s):

Polymerase Chain Reaction ◽

Viral Genome ◽

Foot And Mouth Disease ◽

Enzyme Linked Immunosorbent Assay ◽

Rt Pcr ◽

Chain Reaction ◽

Kappa Value ◽

Polymerase Chain ◽

Pcr Assays ◽

Level Of Agreement

Background: Foot-and-mouth disease (FMD) is an infectious and highly contagious disease of cloven-hoofed domestic and wild animals, causing heavy economic losses to the livestock industry. Rapid and reliable diagnosis of the disease is essential for the implementation of effective control measures. This study compared sandwich enzyme-linked immunosorbent assay (S-ELISA) and conventional reverse transcription polymerase chain reaction (RT-PCR) for the diagnosis of FMD. Methods: A total of 60 epithelial samples from suspected cases of FMD were tested using both S-ELISA and RT-PCR assays. The level of agreement between the assays was assessed by calculating the Kappa value. Results: S-ELISA detected 38 (63%) samples positive for FMD virus (FMDV). Being predominant, serotype O was detected in 22 (57.9%) of the total samples tested positive, whereas 9 (23.7%) and 7 (18.4%) samples were found positive for serotypes A and Asia-1, respectively. RT-PCR detected viral genome in 51 (85%) of the samples using pan-FMDV primers set, 1F/1R. Thirty-six samples were found positive and 7 negative by both the tests. The level of agreement between the tests was assessed by calculating the Kappa value, which was found to be fair (Kappa value = 0.303 and 95% CI = 0.089; 0.517) and significant (p = 0.009). However, 2 samples, which were found positive on S-ELISA tested negative on RT-PCR. This may be attributed to the presence of nucleotide mismatch(es) in the primer-binding sites that may have resulted in failure of amplification of the viral genome. The serotype-specific RT-PCR assays not only confirmed serotyping results of S-ELISA but were also able to establish serotype in 9 S-ELISA-negative but pan-FMDV RT-PCR-positive samples. Conclusions: The RT-PCR assay contributes significantly to establishing a quick, sensitive, and definitive diagnosis of FMD in resource-constrained countries. Samples giving negative results in S-ELISA should be tested in RT-PCR for the disease detection and virus typing.

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