scholarly journals EFFECT OF GYMNEMA SYLVESTRE METHANOLIC EXTRACT ON PANCREATIC PUTRESCINE LEVELS AGAINST ALLOXAN-INDUCED DIABETIC RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 253
Author(s):  
Jothi Muniyandi M ◽  
Benno Susai Vijayakumar A
Keyword(s):  
High Performance ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Gymnema Sylvestre ◽  
Standard Drug ◽  
Beneficial Effects ◽  
Body Weights

Objective: Gymnema sylvestre, a rare herb with significant medicinal values widely used as a naturopathic treatment for diabetes. This study demonstrates the antidiabetic potential and changes in the levels of pancreatic putrescine in G. sylvestre treated experimental rat models.Methods: Phytochemical analysis was carried out for methanolic leaf extract of G. sylvestre (GSME). Further, antidiabetic property and putrescine levels in alloxan-induced diabetic Wister albino rats were analyzed by high-performance liquid chromatography method.Results: The GSME (500 mg/kg b/w) was administered daily up to 15 days in diabetic rats against standard drug metformin (500 mg/kg b/w). At the end of the experiments, the increased body weights were noted in the diabetic control groups, while there was reduction in blood glucose level, total protein, liver glycogen, glutathione, glutathione peroxidase, and blood catalase, respectively.Conclusion: GSME at the dose of (500 mg/kg b/w) brings about significant beneficial effects in various physiological parameters and level of putrescine altered during diabetic manifestations, and these effects are quite comparable with the standard drug, metformin.

scholarly journals EFFECT OF GYMNEMA SYLVESTRE METHANOLIC EXTRACT ON PANCREATIC PUTRESCINE LEVELS AGAINST ALLOXAN-INDUCED DIABETIC RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 253
Author(s):  
Jothi Muniyandi M ◽  
Benno Susai Vijayakumar A
Keyword(s):  
High Performance ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Gymnema Sylvestre ◽  
Standard Drug ◽  
Beneficial Effects ◽  
Body Weights

Objective: Gymnema sylvestre, a rare herb with significant medicinal values widely used as a naturopathic treatment for diabetes. This study demonstrates the antidiabetic potential and changes in the levels of pancreatic putrescine in G. sylvestre treated experimental rat models.Methods: Phytochemical analysis was carried out for methanolic leaf extract of G. sylvestre (GSME). Further, antidiabetic property and putrescine levels in alloxan-induced diabetic Wister albino rats were analyzed by high-performance liquid chromatography method.Results: The GSME (500 mg/kg b/w) was administered daily up to 15 days in diabetic rats against standard drug metformin (500 mg/kg b/w). At the end of the experiments, the increased body weights were noted in the diabetic control groups, while there was reduction in blood glucose level, total protein, liver glycogen, glutathione, glutathione peroxidase, and blood catalase, respectively.Conclusion: GSME at the dose of (500 mg/kg b/w) brings about significant beneficial effects in various physiological parameters and level of putrescine altered during diabetic manifestations, and these effects are quite comparable with the standard drug, metformin.

scholarly journals Effects of a Fraction of the Methanol Extract of the Seeds of Abrus Precatorius on Malondialdehyde and Antioxidant Levels of Alloxan-Induced Diabetic Rats

2021 ◽  
pp. 35-51
Author(s):  
Ebele Lauretta Iloanya ◽  
Okwesili Fred Chiletugo Nwodo ◽  
Obiajulu Christian Ezeigwe ◽  
Ogochukwu Prince Alaebo ◽  
Nnanyelugo Benneth Enemchukwu ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Intraperitoneal Administration ◽  
Diabetic Rats ◽  
Diagnostic Methods ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Enzymatic Antioxidants ◽  
Standard Drug ◽  
Abrus Precatorius

Introduction: The aim of this study is to evaluate the effect of fraction 2 (F2) of methanol extract of the seeds of Abrus precatorius on malondialdehyde and antioxidant level of alloxan-induced diabetic Wistar albino rats. Materials and Methods: The methanol extract of the seeds of A. precatorius Linn Fabaceae was fractionated by Sephadex G15. Diabetes was induced by a single intraperitoneal administration of alloxan at a dose of 150 mg/kg bodyweight. The phytochemical analysis and the biochemical parameters were investigated using standard diagnostic methods. Results: Both qualitative and quantitative analysis of F2 revealed the presence of high amounts of alkaloids (2000 ± 80 mg/100 g), flavonoids (158 ± 17.6 mg/100 g) and tannins (258 ± 45 mg/100 g) but low concentration of saponins (18.3 ± 2.43 mg/100 g). The malondialdehyde (MDA) level of all the test groups and the group treated with the standard drug decreased significantly (p<0.05) compared with that of untreated diabetic group. However, the catalase activity in all the test groups significantly increased (p<0.05) while the activity of superoxide dismutase increased significantly (p<0.05) in groups administered 20mg/kg of the fraction and the group pre-treated with 10mg/kg of the fraction compared with the diabetic untreated group. Similarly, the result revealed a significant increase (p<0.05) in non-enzymatic antioxidants such as reduced glutathione and vitamin E of the rats treated with graded doses of F2 while vitamin C showed significant (p<0.05) increase in normal control groups and pre-treated groups compared with the diabetic untreated. Studies on membrane stabilization using hypotonicity-induced red blood cell haemolysis revealed that the F2 of the methanol extract of the seeds of A. precatorius Linn inhibited haemolysis in a dose-dependent manner. Conclusion: The results obtained from this study revealed that the F2 fraction of the methanol extract of A. precatorius contain important phytochemicals found in the Fraction 2 (F2) however showed improvement in antioxidant defence as well as has a stabilizing effect on the membrane.

scholarly journals Normoglycaemic, Normolipidaemic and Antioxidant Effects of Ethanolic Extract of Acacia ataxacantha Root in Streptozotocin - induced Diabetic Rats

2016 ◽  
Vol 8 (2) ◽  
pp. 144-150
Author(s):  
Rotimi Olusanya ARISE ◽  
Tosan AKAPA ◽  
Moshood A. ADIGUN ◽  
Abeeb Abiodun YEKEEN ◽  
Oluwafemi O. OGUNTIBEJU
Keyword(s):  
Low Density Lipoprotein ◽  
Antioxidant Properties ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Body Weights ◽  
Repeated Doses ◽  
Phytochemical Constituents

The antidiabetic, normolipidaemic, antioxidant and safety evaluations of ethanolic extract of Acacia ataxacantha roots (EEAAR) were investigated in streptozotocin - induced diabetic rats, to verify its use in traditional African medicine and as alternative to synthetic normoglycaemic agents in diabetic treatments. Thirty albino rats (Rattus novergicus) were randomized into six groups - control, diabetic control, EEAAR-treated at 125 mg/kg, 250 mg/kg, 500 mg/kg body weights (b.wts.) and metformin groups, respectively. Phytochemical screening showed the presence of alkaloids, polyphenols, flavonoid, saponins, tannins and terpenoid. Blood glucose was significantly reduced (p < 0.05) especially after 7 days of oral administration of EEAAR at 125 mg/kg b.wt with values (110.01 ± 9.64 mg/dl) similar to that of the control (106.33 ± 4.13 mg/dl). There was an increase (p < 0.05) in the ALT and AST activities of the liver and serum of rats in all the groups except in those that received 125 mg/kg b.wt. Serum total cholesterol, low density lipoprotein cholesterol and triglyceride were decreased (p < 0.05) upon administration of the extract and metformin. There was no difference (p > 0.05) in malondialdehyde concentration of rats administered with 125 mg/kg b.wt. of extract and metformin. Superoxide dismutase activity was elevated (p < 0.05) in all groups and compared favourably with the control in each of the tissues. This study revealed the antidiabetic and hypolipidaemic effects of EEAAR, which may be due to the antioxidant properties of some of the phytochemical constituents. However, the extract may not be safe at large and repeated doses.

scholarly journals Evaluation of antidiabetic activity of Tinospora cardifolia in alloxan induced diabetes in albino wistar rats

2018 ◽  
Vol 7 (7) ◽  
pp. 1382 ◽  
Author(s):  
Abhijith L. M. ◽  
Ravi K. Sori
Keyword(s):  
Therapeutic Potential ◽  
Test Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Standard Drug ◽  
Group I

Background: The objective was to evaluate the antidiabetic activity of Tinospora cardifolia in alloxan induced diabetes in albino rats in comparison with a currently used oral hypoglycaemic glibenclamide.Methods: there were 24 rats with FBS in the range 80-115 mg/dl were selected for the study. Four groups each containing six rats, were induced diabetes with alloxan (150mg/kg). The diabetic control group (0.5ml normal saline), Standard control group (5mg/kg glibenclimide), Test group I (200mg/kg T. cardifolia) and test II group 400mg/kg T. cardifolia). FBS was recorded on 1, 3, 7, 14, 21 and 28th day using glucometer. Data was analysed by using one way ANOVA and posthoc Tukey’s test SPSS 21Version.Results: Extract of Tinospora cardifolia showed dependent hypoglycaemic action in both low dose (200mg/kg) and high dose group (400mg/kg). Hypoglycaemic action with high dose of Tinospora cardifolia is comparable to that of standard drug glibenclamide.Conclusions: This study demonstrates the hypoglycaemic action of T. cardifolia in diabetic rats. T. cardifolia can be a therapeutic potential to treat type 2 diabetes mellitus.

scholarly journals EFFECT OF PITHECELLOBIUM DULCE BENTH LEAVES IN DEXAMETHASONE INDUCED DIABETIC RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 317 ◽  
Author(s):  
Mule V. S. ◽  
Naikwade N. S. ◽  
Magdum C. S. ◽  
Jagtap V. A.
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Glycogen Level ◽  
Standard Drug ◽  
Diabetic Control Group

<p><strong>Objective: </strong>The objective of the present study was to study the effect of <em>Pithecellobium dulce </em>Benth (<em>P. dulce</em>) leaves in dexamethasone-induced diabetic rats.</p><p><strong>Methods: </strong>The authenticated <em>P. dulce</em> leaves were collected from a local area of Sangli, Maharashtra. The leaves of the plant were extracted with water and ethanol by maceration and soxhelation respectively. Acute toxicity studies of the both extracts were performed using rat and according to OECD 425 guidelines. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. The albino rats were divided into seven groups with five animals in each group. The diabetes was induced by dexamethasone (10 mg/kg, s. c.) and treated with extract and standard drug for 10 d. Then blood glucose, triglyceride, total cholesterol and glycogen level in liver, muscle and kidney were estimated according to standard procedures.</p><p><strong>Results: </strong>The study revealed that <em>P. dulce</em> at 200 mg/kg and 400 mg/kg showed significant (p ˂ 0.05) antidiabetic activity. All the extract treated groups showed a significant reduction in blood glucose level on 11<sup>th</sup> day when compared to diabetic control group. The significant increase in blood glucose, triglyceride, and total cholesterol level was observed in the diabetic control group when compared to normal control group. The liver and muscle glycogen level was decreased significantly (p ˂ 0.05) in the diabetic control group.<strong></strong></p><strong>Conclusion: </strong>It can be concluded that <em>P. dulce</em>aqueous and ethanolic extract at two different doses (200 mg/kg and 400 mg/kg) possesses antidiabetic and hypolipidemic activity.<p> </p>

scholarly journals Ameliorative Effects of Aloe Vera Gel Extract on Circulatory and Tissue Lipid Profile Status in Streptozotocin-Induced Diabetic Rats

2018 ◽  
Vol 9 (1) ◽  
pp. 11-26
Author(s):  
RN Ugbaja ◽  
DI Akinloye ◽  
AJ Akamo ◽  
EI Ugwor ◽  
OO IIesanmi ◽  
...  
Keyword(s):  
Aloe Vera ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipid Profiles ◽  
Albino Rats ◽  
Tissue Lipid ◽  
Rbc Membrane ◽  
Aloe Vera Gel ◽  
Lipid Parameters ◽  
Scientific Rationale

Diabetes is characterized by hyperglycaemia with alterations of lipid parameters. The effects of Aloe vera (A.V) gel extract on lipid profiles of streptozotocin-induced diabetic rats were investigated. Seventy-two male albino rats were randomly divided into six groups of 12 rats each; control, diabetic control, 4 ml/kg and 8 ml/kg body weight (b.w.) A.V, 10 mg/kg b.w. glibenclimide and non-diabetic 8 ml/kg b.w. A.V groups. They were treated for 14 and 28 days. The plasma and HDL lipid profiles (triacylglycerols, cholesterol and phospholipids) of the diabetic rats were significantly (p<0.05) increased, while those of the VLDL+LDL, RBC, RBC membrane and organs were observed to be significantly (p<0.05) reduced, when compared to the control after 14 and 28 days of treatment. A.V gel extract significantly (p<0.05) ameliorated the disruptions observed in the lipid profiles of the diabetic and thus provide a scientific rationale for the use of Aloe vera as a therapeutic agent.Bangladesh J Med Biochem 2016; 9(1): 11-26

scholarly journals Hypoglycemic Potential of Dietary Supplementation of Protein Isolate from Fermented Cucumeropsis manii in Streptozotocin Induced Hyperglycemic in Male Wistar Albino Rats

2021 ◽  
pp. 31-42
Author(s):  
A. O. Abiola ◽  
A. O. Iyoribhe ◽  
S. A. Adeniyi ◽  
O. B. Adu ◽  
A. S. Ogunbowale ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Enzyme Activities ◽  
Cvd Risk ◽  
Streptozotocin Induced Diabetes

The effect of Protein isolate from fermented melon seeds (Ogiri Protei Isolates; OPI) of Cucumeropsis manii on blood glucose, lipid profile, and antioxidant enzyme activities in streptozotocin (STZ)-induced diabetic rats was investigated. Thirty Male Wistar rats were divided into five equal groups. GThe first control group with no exposure. The second group of rats with Streptozotocin-induced non-treated diabetes. The 3rd and 4th groups of rats with Streptozotocin-induced diabetes supplemented with Ogiri protein isolates (200, 600 mg/kg in diet). And the 5th group of rats with Streptozotocin-induced diabetes administered glibenclamide in a dose 500 ug/kg in diet [17]. The OPI was administered for 6 weeks. The administration of OPI reduced the blood glucose concentration of the STZ-induced diabetic rats. Sera and hepatic superoxide dismutase, activities of the STZ-induced diabetic rats were significantly (P< 0.05) increased in comparison with the diabetic control rats. Lipid peroxidation of the supplemented OPI diabetic rats was significantly (P< 0.05) decreased in comparison with the diabetic control rats as the administration of OPI to the STZ-induced diabetic rats significantly increased the enzymes’ activities. The concentration of low-density lipoproteins in the OPI supplemented rats was significantly elevated. These data demonstrate that OPI supplements might be beneficial for correcting hyperglycemia but the consumption of OPI can modulate some tissue lipids in a direction not beneficial for CVD risk in patients with diabetes.

scholarly journals Antidiabetic potential of the leaf extract of Centella asiatica in alloxaninduced diabetic rats

2016 ◽  
Vol 4 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Talha Bin Emran ◽  
Mycal Dutta ◽  
Mir Muhammad Nasir Uddin ◽  
Aninda Kumar Nath ◽  
Md Zia Uddin
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat

The study was designed to evaluate the glucose and cholesterol lowering effect of the aqueous extract of Centella asiatica leaf using the alloxan-induced diabetic rats and compared the activity with diabetic control and antidiabetic drug (Glibenclamide). Leaf extract (50 mg/kg) of C. asiatica and Glibenclamide were administered to normal and experimental diabetic rats for the duration of 10 days. In the alloxan-induced diabetic rat model, C. asiatica extract (50 mg/kg) significantly (p < 0.05) lowered the fasting blood glucose level as well as the total cholesterol level. Serum insulin levels were not stimulated in the animals treated with the extract. In addition, changes in body weight, serum lipid profiles and liver glycogen levels assessed in the extract treated diabetic rats were compared with diabetic control and normal animals. Significant results (p < 0.05) were observed in the estimated parameters. Surprisingly, body weight was increased significantly (p < 0.05) in the C. asiatica treated diabetic group. Phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, steroids and tannins in significant amountsJahangirnagar University J. Biol. Sci. 4(1): 51-59, 2015 (June)

scholarly journals Amelioration of hyperglycaemia and its associated complications by finger millet (Eleusine coracanaL.) seed coat matter in streptozotocin-induced diabetic rats

2010 ◽  
Vol 104 (12) ◽  
pp. 1787-1795 ◽  
Author(s):  
Shanmugam Shobana ◽  
Mysore R. Harsha ◽  
Kalpana Platel ◽  
Krishnapura Srinivasan ◽  
Nagappa G. Malleshi
Keyword(s):  
Seed Coat ◽  
Finger Millet ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Tubular Structures ◽  
Glycation End Products ◽  
Body Weights ◽  
Millet Seed ◽  
Effect Of Feeding ◽  
Partial Reversal

Finger millet (Eleusine coracana) is extensively cultivated and consumed in India and Africa. The millet seed coat is a rich source of dietary fibre and phenolic compounds. The effect of feeding a diet containing 20 % finger millet seed coat matter (SCM) was examined in streptozotocin-induced diabetic rats. Diabetic rats maintained on the millet SCM diet (diabetic experimental (DE) group) for 6 weeks exhibited a lesser degree of fasting hyperglycaemia and partial reversal of abnormalities in serum albumin, urea and creatinine compared with the diabetic control (DC) group. The DE group of rats excreted comparatively lesser amounts of glucose, protein, urea and creatinine and was accompanied by improved body weights compared with their corresponding controls. Hypercholesterolaemia and hypertriacylglycerolaemia associated with diabetes were also notably reversed in the DE group. Slit lamp examination of the eye lens revealed an immature subcapsular cataract with mild lenticular opacity in the DE group of rats compared to the mature cataract with significant lenticular opacity and corneal vascularisation in the DC group. Lower activity of lens aldose reductase, serum advanced glycation end products and blood glycosylated Hb levels were observed in the DE group. The millet SCM feeding showed pronounced ameliorating effects on kidney pathology as reflected by near normal glomerular and tubular structures and lower glomerular filtration rate compared with the shrunken glomerulus, tubular vacuolations in the DC group. Thus, the present animal study evidenced the hypoglycaemic, hypocholesterolaemic, nephroprotective and anti-cataractogenic properties of finger millet SCM, suggesting its utility as a functional ingredient in diets for diabetics.

scholarly journals Hypoglycaemic and Hypolipidemic Effects of Treculia africana Aqueous Leaves Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
Idris A. Kankara ◽  
Gayus A. Paulina ◽  
M. Aliyu
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Fasting Blood Glucose ◽  
Hdl Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Treculia Africana

This study investigated the hypoglycaemic and hypolipidaemic effects of Treculia africana plant used in Nigeria as medicinal plant. Diabetes mellitus was induced by a single dose intraperitoneal injection of alloxan 150 mg/kg body weight. Twenty five (25) male albino rats were divided into five groups, five (5) rats per group; normal control, diabetic control and diabetic groups treated with aqueous leaves extract of 200,400 and 800 mg/Kg body weight respectively for 21 days orally. The effects of the extract on some biochemical parameters were evaluated; fasting blood glucose level was assayed using glucose oxidase method, total cholesterol and HDL –cholesterol were assayed using enzymatic method while LDL- cholesterol was determined by Friedewald equation. The results showed that, extract significantly (p<0.05) decrease the elevated fasting blood glucose levels, total cholesterol, triglyceride and LDL- cholesterol when compared with the diabetic control rats. The extract also caused significant (p<0.05) increased in HDL –cholesterol and body weight when compared with diabetic control rats. Aqueous leave extract of Treculia africana possess hypoglycemic effect and the most effective dose was 800 mg/Kg body weight in amelioration of hyperglycaemia and most all toxicity effects of alloxan on lipid profile.

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