scholarly journals ACTIVITY OF 96% ETHANOL EXTRACT OF CHRYSOPHYLLUM CAINITO L. IN INCREASING VERTEBRAE TRABECULAR OSTEOBLAST CELL NUMBER IN MALE MICE

2019 ◽  
Vol 12 (1) ◽  
pp. 286
Author(s):  
Burhan Ma'arif ◽  
Agnis Pondinekaria Aditama
Keyword(s):  
Effective Dose ◽  
Ethanol Extract ◽  
Optical Microscope ◽  
Cell Number ◽  
Positive Control ◽  
Osteoblast Cell ◽  
Male Mice ◽  
Chrysophyllum Cainito ◽  
Zoom In ◽  
Hematoxylin Eosin

Objectives: This study was conducted to analyze the anti-osteoporosis effect of 96% ethanol extract from Chrysophyllum cainito L. leaves, which suspected to contain phytoestrogens, in increasing the osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice.Materials and Methods: The 96% ethanol extract of C. cainito leaves was given to male mice with dose of 2, 4, 8, and 16 mg/20 g BB mice/day after being induced orally with dexamethasone dose of 0.0029 mg/20 g BB mice/day. The positive control used was 0.026 ml/20 g BB mice/day alendronate. After 4 weeks, the increasing of osteoblast cell number in trabecular vertebrae bone of male mice was observed using an optical microscope with 100× zoom in after histomorphometry and hematoxylin-eosin staining methods.Results: The result showed the significant increasing of osteoblast number in trabecular vertebrae bone of male mice in all groups after being given treatment using 96% ethanol extract of C. cainito, with an effective dose (ED50) value of 9.5 mg/20 g BB mice/day. This increase is suspected due to the phytoestrogens content, which can also act as phytotestosterone in 96% ethanol extract of C. cainito.Conclusion: This study concluded that 96% ethanol extract of C. cainito has an activity in increasing osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice, with an ED50 value of 9.5 mg/20 g BB mice/day.

scholarly journals ACTIVITY OF 96% ETHANOL EXTRACT OF CHRYSOPHYLLUM CAINITO L. IN INCREASING VERTEBRAE TRABECULAR OSTEOBLAST CELL NUMBER IN MALE MICE

2019 ◽  
Vol 12 (1) ◽  
pp. 286
Author(s):  
Burhan Ma'arif ◽  
Agnis Pondinekaria Aditama
Keyword(s):  
Effective Dose ◽  
Ethanol Extract ◽  
Optical Microscope ◽  
Cell Number ◽  
Positive Control ◽  
Osteoblast Cell ◽  
Male Mice ◽  
Chrysophyllum Cainito ◽  
Zoom In ◽  
Hematoxylin Eosin

Objectives: This study was conducted to analyze the anti-osteoporosis effect of 96% ethanol extract from Chrysophyllum cainito L. leaves, which suspected to contain phytoestrogens, in increasing the osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice.Materials and Methods: The 96% ethanol extract of C. cainito leaves was given to male mice with dose of 2, 4, 8, and 16 mg/20 g BB mice/day after being induced orally with dexamethasone dose of 0.0029 mg/20 g BB mice/day. The positive control used was 0.026 ml/20 g BB mice/day alendronate. After 4 weeks, the increasing of osteoblast cell number in trabecular vertebrae bone of male mice was observed using an optical microscope with 100× zoom in after histomorphometry and hematoxylin-eosin staining methods.Results: The result showed the significant increasing of osteoblast number in trabecular vertebrae bone of male mice in all groups after being given treatment using 96% ethanol extract of C. cainito, with an effective dose (ED50) value of 9.5 mg/20 g BB mice/day. This increase is suspected due to the phytoestrogens content, which can also act as phytotestosterone in 96% ethanol extract of C. cainito.Conclusion: This study concluded that 96% ethanol extract of C. cainito has an activity in increasing osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice, with an ED50 value of 9.5 mg/20 g BB mice/day.

scholarly journals Utilizing of 96% Etanol Extract Activity Red Leaf (Syzygium myrtifolium Walp.) Maseration and Socletation Method as an Antidiarrhea at Male Mice

2019 ◽  
Vol 8 (2S7) ◽  
pp. 260-263
Keyword(s):  
Effective Dose ◽  
Ethanol Extract ◽  
Extraction Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Traditional Medicines ◽  
Intestinal Length ◽  
Child Morbidity ◽  
Ethanol Extracts

Diarrhea is still one of the most important public health problems because it is the third major contributor to child morbidity and mortality in Indonesia. One of the traditional medicines that can be used to treat diarrhea is red shoots. Red shoot leaves contain secondary metabolites such as alkaloids, flavonoids, chalcones, tannins, and terpenoids which can be used as antidiarrheals. The purpose of this study was to compare the antidiare effects between extracts of maceration and socletation of red shoot leaves and determine the effective dose of both extracts as antidiarrheal in male mice using the intestinal transit method. The treatment in this study consisted of the administration of ethanol extract of shoots of red shoots by maceration at a dose of 1.58 mg / 20 g BW (dose of MI), 4.75 mg / 20g BW (dose of M.II), 6.33 mg / 20g BB (Dosage M.III) and ethanol extract of shoots of red shoots by socletation at a dose of 1.67 mg / 20 g BB (SI dose), 5.04 mg / 20 g BB (Dosage S.II), 6.72 mg / 20 g BB (Dose S.III), Loperamid HCl as positive control, and 1% CMC suspension as negative control. Observations were made by measuring the ratio of intestinal length traveled through the norit (marker) to the total gut length. The results showed that all dose treatments on maceration and socletation ethanol extracts had antidiareal activity. Different extraction methods give the same effect in giving antidiarrheal effects. Based on Duncan's advanced dose of 6.72 mg / 20 g BW of socletation results is an effective dose as antidiarrheal because it has an effect that is not significantly different than Loperamid HCl.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Aktivitas Ekstrak Etanol Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Penyembuhan Luka Sayat pada Tikus Putih (Rattus Norvegicus L.)

2018 ◽  
Vol 4 (2) ◽  
pp. 113-118
Author(s):  
Umi Calsum ◽  
Akhmad Khumaidi ◽  
Khildah Khaerati
Keyword(s):  
Effective Dose ◽  
Rattus Norvegicus ◽  
Povidone Iodine ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Healing Time ◽  
White Rats ◽  
Lannea Coromandelica ◽  
Healing Wound

Jawa bark (Lannea coromandelica) is a part of the plant that can be used empirically for handling wounds. This study aims to determine the activity of ethanol extract of Jawa bark on healing wound and determine the effective dose compared with povidone iodine. The test animals used were white rats (Rattus norvegicus L.) consisting of 5 groups, namely negative control (vaseline without extract), positive control (povidone iodine), dose of 250 mg/kg BW, dose of 500 mg/kg BW, dose of 750 mg/kg BW. Each rat made an incision in the area parallel to the spine with 2 cm long and 2 mm deep. Measuring the length of the wound was done every day for 14 days. The data obtained were analyzed statistically using One Way Anova and followed by Post Hoc Duncan test. Statistical results showed that the ethanol extract of Jawa bark has an activity in curing slice where the effective dose is a dose of 500 mg/kg BW with a wound healing time of 12 days.

scholarly journals ANTIMUTAGENIC ACTIVITY OF BINARA HERBS (ARTEMISIA VULGARIS L.) ETHANOL EXTRACT IN MALE MICE INDUCED BY CYCLOPHOSPHAMIDE

2018 ◽  
Vol 11 (4) ◽  
pp. 236 ◽  
Author(s):  
Linta Meliala ◽  
Urip Harahap ◽  
Urip Harahap ◽  
Aminah Dalimunthe ◽  
Aminah Dalimunthe
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Test Method ◽  
Antimutagenic Activity ◽  
Male Mice ◽  
Artemisia Vulgaris ◽  
Polychromatic Erythrocytes ◽  
Suspension Preparation

 Objectives: This study aims to evaluate the antimutagenic activity of binara herbs (Artemisia vulgaris L.) ethanol extract on male mice.Methods: Binara herbs powder was extracted is used ethanol 96% by maceration, then made into a suspension preparation used 0.5% carboxymethylcellulose with 3 dose variations (100, 200, and 300 mg/kg). Further, the extract was administrated orally in mice for 7 consecutive days. 4 h after the last orally, cyclophosphamide 50 mg/kg IP was injected. 30 h after cyclophosphamide injection, the animals were killed, and the samples of bone marrow were prepared and stained with gems. For each sample, 200 cells of polychromatic erythrocytes (PCE) and the same number of normochromatic erythrocytes (NCE) and the cells containing their micronucleus (MN) were counted using the MN test method in vivo.Results: Cyclophosphamide increased the frequency of MnPCE and decreased cell proliferation (PCE/PCE+NCE). All doses of extracts significantly reduced the frequency of MnPCE (p<0.05). The cell proliferation ratio (PCE/PCE+NCE) was also increased. The most effective dose is 300 mg/kg because it has the greatest ability to decrease the frequency of MnPCE and increase the cell proliferation ratio.Conclusion: Results of this study indicated that binara herbs (A. vulgaris L.) ethanol extract has potent antimutagenic activity.

scholarly journals 33. Efficacy of Jatropha(Jatrophacurcas L.) Sap Cream in Inflammation Phase of Wound Healing on Mice Skin

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Hidayati Hidayati ◽  
Muhammad Nur Salim ◽  
Ummu Balqis ◽  
Dian Masyitha ◽  
Cut Dahlia Iskandar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Inflammatory Cells ◽  
Positive Control ◽  
Control Treatment ◽  
Male Mice ◽  
Skin Sample ◽  
Inflammatory Phase ◽  
Speed Up ◽  
Hematoxylin Eosin

This study aim to know the efficacy of the jatropha sap cream toward the inflammatory phase of wound healing on the skin of mice. This study used 9 male mice, with 25-40 grams weights and 2-3 months old.  Male mice receiveddivided into 3 groups, and3 deuteronomy.The cut is done in the back as 2 cm long. Wound care is done twice a day at 08.00 am and at 18.00 pm for 3 days. The treatment I (PI) cuts smeared a cream base (as a negatif control); treatment II (PII) smeared 10% jatropha sap cream; and the treatment III (PIII) smeared 0.1% sulfadiazine  (as a positive control).Created a skin sample preparation microtechnic stained with hematoxylin-eosin (HE). The result on the number of infiltration of inflammatory cells and neovascular on PI; PII; and PIII were 402,00 ± 7,21 and 14,00 ± 5,57; 248,67 ± 1,53 and 34,67 ± 7,51; and 343,00 ± 8,89 and 28,00 ± 5,00  (P0,01). Based on the result it be concluded the treatment of 10% jatropha sap cream can speed up the process of the inflammatory phase of wound healing in the skin of mice.

scholarly journals Activity of 70% Ethanol Extract of Chrysophyllum cainito in Increasing Vertebrae Trabecular Bone Density in Female Mice

2018 ◽  
Vol 23 (3) ◽  
pp. 119 ◽  
Author(s):  
Nur Imamah Utaminingtyas ◽  
Burhan Ma'arif ◽  
Dewi Sinta Megawati ◽  
Ria Ramadhani Dwi Atmaja
Keyword(s):  
Bone Density ◽  
Trabecular Bone ◽  
Ethanol Extract ◽  
Positive Control ◽  
Optimum Dose ◽  
Trabecular Bone Density ◽  
Female Mice ◽  
Staining Methods ◽  
Chrysophyllum Cainito

Phytoestrogens are a class of compounds that have estrogen-like functions or structure. This research was conducted to find out the phytoestrogens activity in Chrysophyllum cainito leaves, in increasing the vertebrae trabecular bone density of female mice, using dexamethasone induction as osteoporosis model.  The 70% ethanol extract of C. cainito leaves was given to female mice with dose of 100; 200; and 400 mg/kgBW mice/day after being induced orally with dexamethasone with 0.145 mg/kgBW mice/day. The positive control used was 1.3 mg/kgBW mice/day alendronate. This research had been done in four weeks. The increasing of trabecular vertebrae bone density of female mice was measured with Histomorphometry staining methods. The result showed the 70% ethanol extract of C. cainito leaves have activity in increasing trabecular vertebrae bone density for female mice with optimum dose of 400 mg/kgBW mice/day. This activity occurs because phytoestrogens content in 70% ethanol extract of C. cainito leaves, that may replace the function of estrogen in its bond with ER.

scholarly journals NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF CURCUMA MANGGA VAL. IN PARACETAMOL-INDUCED MALE MICE

2018 ◽  
Vol 11 (13) ◽  
pp. 126
Author(s):  
Rosita Rosita ◽  
Yuandani Yuandani ◽  
Marianne Marianne
Keyword(s):  
Creatinine Level ◽  
Cell Damage ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dependent Manner ◽  
Male Mice ◽  
Treatment Groups ◽  
Level Measurement ◽  
Nephroprotective Activity

Objective: This study aimed to evaluate nephroprotective activity of Curcuma mangga in paracetamol-induced male mice.Methods: Male mice were divided into several groups including normal control, negative, positive, and treatment groups. Treatment groups were orally administered with C. mangga extract at doses of 100, 200, and 400 mg/kg bw for 7 days. On day 7after 1 h of treatment, the mice were induced with paracetamol 1.05 g/kg bw. Serum creatinine level measurement and histopathotlogy study were performed at the end of experiment.Results: C. mangga extract was able to inhibit the increase of creatinine level and showed a significantly different from negative control (p<0.05) and did not different significantly from positive control (p>0.05). The result was supported by histopathology examination which did not show any cell damage. The nephroprotective effect of C. mangga was in a dose-dependent manner. C. mangga extract at dose of 400 mg/kg bw depicted the strongest nephroprotective effect.Conclusion: C. mangga extract was able to protect mice kidney induced by paracetamol.

scholarly journals ALOE VERA ETHANOL EXTRACT AS A THERAPY FOR ALVEOLAR MANDIBLE REGENERATION IN LIPOPOLYSACCHARIDE-EXPOSED RATS

2018 ◽  
Vol 10 (1) ◽  
pp. 11
Author(s):  
Latifah Fitriani Rakhman ◽  
Octaviana Widya Pangestika ◽  
Delvi Fitriani ◽  
Rudhanton Rudhanton ◽  
Nur Permatasari
Keyword(s):  
Alveolar Bone ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Alveolar Tissue ◽  
Hematoxylin Eosin ◽  
One Way Anova

Objective: The study examined the effects of Aloe vera ethanol extract on alveolar mandible regeneration in rats (Rattus norvegicus).Methods: Rats were divided into five groups as follows: Negative control group received no treatment, positive control group received Escherichiacoli lipopolysaccharide (LPS) alone for 5 days, and Groups P1, P2, and P3 received LPS for 5 days followed by 40, 80, and 160 mg/kg BW A. veraethanol extract, respectively, orally on days 6–12. Alveolar bone tissues were taken and histologically processed through hematoxylin-eosin staining.Osteoblast and osteoclast numbers in alveolar tissue were also examined.Results: One-way ANOVA revealed strong relationships between the A. vera ethanol extract dosage and the numbers of osteoblasts (r=0.921, p<0.05)and osteoclasts (r=−0.631, p<0.05) in rats.Conclusion: A. vera ethanol extract appears capable of stimulating alveolar bone regeneration following LPS exposure.

scholarly journals Anti-inflammatory Activity of Ethanol Extract from Malacca Leaves (Phyllanthus emblica) in Carrageenan Induced Male Mice

2020 ◽  
Vol 151 ◽  
pp. 01066
Author(s):  
Nuzul Asmilia ◽  
Amalia Sutriana ◽  
Dwinna Aliza ◽  
Nurfadilla Sudril
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Phyllanthus Emblica ◽  
Male Mice ◽  
Edema Volume ◽  
Volume Measurements ◽  
Group 2

The purpose of this study was to determine the effect of the ethanol extract of malacca leaves (Phyllanthus emblica) to reduce carrageenan-induced edema in mice. This study used 25 mice which were divided into 5 groups with 5 mice per group. All the mice were induced inflammation using carrageenan at a dose of 1%. Mice in group 1 were not given piroxicam and malacca leaves extract. Mice in group 2 were treated with 20 mg of piroxicam suspension. Mice in groups 3, 4, and 5 were given ethanol extract of malacca leaves with doses of 100 mg/kg bb, 200 mg/kg bb, and 300 mg/kg bb, respectively. Edema volume measurements were performed on 5th day by collecting exudates using a syringe. Data were analyzed using one-way analysis of variance and continued with the Duncan test. The result showed that the average of edema volume in the negative control group was 0.09 ± 0.02 mL, while in the positive control group was 0.05 ± 0.03 mL. The average edema volume in groups 3, 4, dan 5 were 0.07 ± 0.01 mL, 0.06 ± 0.02 mL dan 0.07 ± 0.01 mL, respectively. It is concluded that the ethanol extract of malacca leaves (Phyllanthus emblica) can reduce the carrageenan-induced edema in male mice.

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