scholarly journals NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF CURCUMA MANGGA VAL. IN PARACETAMOL-INDUCED MALE MICE

2018 ◽  
Vol 11 (13) ◽  
pp. 126
Author(s):  
Rosita Rosita ◽  
Yuandani Yuandani ◽  
Marianne Marianne
Keyword(s):  
Creatinine Level ◽  
Cell Damage ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dependent Manner ◽  
Male Mice ◽  
Treatment Groups ◽  
Level Measurement ◽  
Nephroprotective Activity

Objective: This study aimed to evaluate nephroprotective activity of Curcuma mangga in paracetamol-induced male mice.Methods: Male mice were divided into several groups including normal control, negative, positive, and treatment groups. Treatment groups were orally administered with C. mangga extract at doses of 100, 200, and 400 mg/kg bw for 7 days. On day 7after 1 h of treatment, the mice were induced with paracetamol 1.05 g/kg bw. Serum creatinine level measurement and histopathotlogy study were performed at the end of experiment.Results: C. mangga extract was able to inhibit the increase of creatinine level and showed a significantly different from negative control (p<0.05) and did not different significantly from positive control (p>0.05). The result was supported by histopathology examination which did not show any cell damage. The nephroprotective effect of C. mangga was in a dose-dependent manner. C. mangga extract at dose of 400 mg/kg bw depicted the strongest nephroprotective effect.Conclusion: C. mangga extract was able to protect mice kidney induced by paracetamol.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

The enhancement of Arg1 and activated ERβ expression in microglia HMC3 by induction of 96% ethanol extract of Marsilea crenata Presl. leaves

2020 ◽  
Vol 30 (6) ◽  
Author(s):  
Burhan Ma’arif ◽  
Mangestuti Agil ◽  
Hening Laswati
Keyword(s):  
Laser Scanning ◽  
Negative Relationship ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Scanning Microscope ◽  
Treatment Groups ◽  
Arginase 1 ◽  
Significant Difference ◽  
Ifn Γ

AbstractBackgroundPhytoestrogens have a high potential to overcome the neuroinflammation caused by estrogen deficiency. Marsilea crenata Presl. is a plant known to contain phytoestrogens. This research aimed to report the activity of a 96% ethanol extract of M. crenata leaves in inducing activation of microglia HMC3 cell to M2 polarity, which has anti-inflammatory characteristics.MethodsThe study was done by culturing microglia HMC3 cell in 24-well microplate and inducing it with IFN-γ for 24 h to activate the cell to M1 polarity, which has proinflammatory characteristics. The 96% ethanol extract was added with various doses of 62.5, 125, and 250 ppm. Genistein, 50 μM, was used as a positive control. The analysis of the immunofluorescence of Arginase-1 (Arg1) and ERβ as markers was done using a convocal laser scanning microscope.ResultsThe result of Arg1 shows a significant difference in Arg1 expression in the microglia HMC3 cell line between the negative control and all treatment groups at p < 0.05, with the best result at 250 ppm, whereas for ERβ, the results show, at doses of 125 and 250 ppm, that the 96% ethanol extract of M. crenata leaves decrease the activated ERβ expression at p < 0.05, with the best result at 250 ppm. The Arg1 and activated ERβ expression have a weak negative relationship with the Pearson correlation test.ConclusionsThe 96% ethanol extract of M. crenata leaves has an antineuroinflammation activity through the induction of Arg1 and activated ERβ expression in microglia HMC3 cell, with the best dose at 250 ppm.

scholarly journals UJI AKTIVITAS ANTIHIPERURISEMIA EKSTRAK ETANOL DAUN KUMIS KUCING (Orthosiphon aristatus (Blume) Miq.) PADA TIKUS PUTIH JANTAN (Rattus Norvegicus)

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 730
Author(s):  
Frinsia Rutly Mokalu ◽  
Widdhi Bodhi ◽  
Julianri S. Lebang
Keyword(s):  
Uric Acid ◽  
Xanthine Oxidase ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Acid Value ◽  
Positive Control ◽  
Negative Control ◽  
Anova Test ◽  
Antioxidants Activity ◽  
Treatment Groups

ABSTRACT Kumis Kucing Leaves (Orthosiphon aristatus (Blume) Miq.) contains flavonoid compounds that has antioxidants activity that inhibits the action of the enzyme xanthine oxidase with reduced uric acid as result. This research is done to find out the effects antihyperuricemia ethanol extract of Kumis Kucing leaves on male white rat (Rattus norvegicus). 15 rats were used in this research and there were 5 treatment groups that is negative control (NACMC 1%), positive control (Allopurinol) 1,8 mg, and Kumis Kucing leaves extract group with dosage of 4,5 mg, 9 mg, 18 mg. The result of this research showed a decrease on uric acid value after ethanol extract of Kumis Kucing leave were given. The next result of this research used ANOVA test and LSD test, that showed the ethanol extract of Kumis Kucing leave had Antihyperuricemia activity on male white rat.Keywords:  Orthosiphon aristatus (Blume.) Miq., Antihyperuricemia, Rattus norvegicus.   ABSTRAK Daun kumis kucing (Orthosiphon aristatus (Blume) Miq.) mengandung senyawa flavonoid yang mempunyai aktivitas antioksidan yang dapat menghambat kerja enzim xantin oksidase sehingga pembentukan asam urat berkurang. Penelitian ini dilakukan untuk mengetahui efek antihiperurisemia ekstrak etanol daun kumis kucing, terhadap tikus putih jantan (Rattus norvegicus). Penelitian ini menggunakan 15 ekor tikus dan terdapat 5 kelompok perlakuan yaitu kontrol negatif (NaCMC 1%), kontrol positif (Allopurinol) 1,8 mg, dan kelompok ekstrak daun kumis kucing dengan dosis 4,5 mg, 9 mg, 18 mg. Hasil penelitian menunjukkan nilai asam urat mengalami penurunan setelah diberikan ekstrak etanol daun kumis kucing. Hasil penelitian selanjutnya dianalisis menggunakan uji ANOVA dan uji LSD, menunjukkan bahwa ekstrak etanol daun kumis kucing memiliki aktivitas antihiperurisemia terhadap tikus putih jantan. Kata kunci: Orthosiphon aristatus (Blume.) Miq., Antihiperurisemia, Rattus norvegicus

scholarly journals Efek Analgetik Ekstrak Etanol Kelopak Rosella (Hibiscus sabdariffa L) Pada Mencit Jantan (Mus musculus)

2017 ◽  
Vol 8 (2) ◽  
pp. 64
Author(s):  
Ridwan Baihaqi ◽  
M. Yulis Hamidy ◽  
Eka Bebasari
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Prostaglandin Synthesis ◽  
Hibiscus Sabdariffa ◽  
Antipyretic Effect ◽  
Treatment Groups ◽  
Post Test

Indonesia has been known for its traditional medicine and one of the plants used for tradisional medicine is Hibiscussabdariffa L. The roselle (Hibiscus sabdariffa L) calyx has antipyretic effect by blocking prostaglandin synthesis. Thepurpose of this study was to find out the existences of analgesic effect of roselle (Hibiscus sabdariffa L) calyx ethanolextract. This experimental research used a post test only design that was conducted on 25 male mices weighting 20-35 g. The mices were divided into 5 treatment groups and given the CMC-Na 0,5% (negative control), asetosal 65 mg/kgBB (positive control) and groups of roselle (Hibiscus sabdariffa L) calyx ethanol extract dose of 200 mg/kgBB,400 mg/kgBB and 800 mg/kgBB. Pain stimulus was given chemically by using 1% acetic acid that injectedintraperitoneally 30 minutes after sample was given the extract, the response of the mices will be writhing. It calledpositive response if percentage potency of analgesic e” 50%. The result of this research showed that all dosage ofroselle (Hibiscus sabdariffa L) calyx ethanol extract had an analgesic effect.

scholarly journals Pengaruh Pemberian Kombinasi Ekstrak Etanol Daun Sidaguri (Sida rhombifolia L.) dan Rimpang Jahe Merah (Zingiber officinale Rosc.) pada Mencit Jantan Hiperurisemia

2018 ◽  
Vol 6 (2) ◽  
pp. 205
Author(s):  
Kinanthi Putri Rizki ◽  
Siti Muslichah ◽  
Indah Yulia Ningsih
Keyword(s):  
Uric Acid ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Treatment Groups ◽  
Ginger Rhizome ◽  
Red Ginger ◽  
Sida Rhombifolia ◽  
Potassium Oxonate

  This study aimed to determine the effect of the combination of sidaguri leaves and red ginger rhizome extracts in uric acid levels of hyperuricemic male mice. Thirty-six mice were divided into nine groups including normal group, the positive control (+), negative control (-), extracts of four treatment groups with a variety of combinations and two groups of single extract. Hyperuricemia induction was performed by administering mixture melinjo 10% of the standard feed mice for 7 days dan potassium oxonate 250 mg/kg BB intraperitoneally at 2 hours before blood sampling. The results showed that the combination dose of sidaguri leaves of 50 mg/kg BB and red ginger rhizome 400 mg/kg BB had higher antihyperuricemia activity than other combinations in lowering uric acid levels of mice. Compounds may have antihyperuricemic activity were flavonoids, alkaloids, saponins, tannins, and terpenoids.   Keywords: sidaguri leaves, red ginger rhizome, antihyperuricemic activity, uric acid  

UJI AKTIVITAS ANALGETIK EKSTRAK ETANOL HERBA SEMBUKAN (Paederia foetida L.) PADA MENCIT PUTIH JANTAN (Mus musculus) YANG DIINDUKSI DENGAN ASAM ASETAT

2020 ◽  
Vol 5 (2) ◽  
pp. 358-363
Author(s):  
Triswanto Sentat ◽  
◽  
Fitri Handayani ◽  
Ellen Indraswari
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Paederia Foetida

Sembukan plants (Paederia foetida L.) are wild vines that are usually used by people as potential medicinal plants for pain. The purpose of this study was to determine the ethanol extract of sembukan’s herbal analgesic activity in male white mice (Mus musculus) and determine the optimal dose of the ethanol extract from sembukan’s herbal (Paederia foetida L.) which has the potential as an analgesic. Sembukan’s herbal is extracted with 70% ethanol solvent and an analgesic activity test is divided into 5 treatment groups namely positive control group (potassium diclofenac), negative control, dose I (80 mg / kg body weight), dose II (160 mg / kg body weight) ) and dose III (320 mg / kg body weight) by oral administration. Thirty minutes after administration, the mice were given an indicator of pain, 0.5% acetic acid. Analgesic power is calculated by counting the amount of stretching of mice for 1 hour. From the results of the study, the ethanol extract of sembukan’s herbal has analgesic activity in male white mice with percent dose analgesic power 35.18%, dose II 53.58% and dose III 68.98%. The optimal dose that has potential as an analgesic in male white mice is dose III with 68.98% analgesic power.

scholarly journals Antidepressant-Like Effects of Ethanol Extract of Ziziphus jujuba Mill Seeds in Mice

2020 ◽  
Vol 10 (20) ◽  
pp. 7374
Author(s):  
Jong Min Oh ◽  
Moongi Ji ◽  
Mi-Jin Lee ◽  
Geum Seok Jeong ◽  
Man-Jeong Paik ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Tail Suspension Test ◽  
Positive Control ◽  
Negative Control ◽  
Mild Stress ◽  
Dependent Manner ◽  
Behavioral Tests ◽  
Bdnf Expression ◽  
Ziziphus Jujuba ◽  
Negative Controls

The antidepressant-like activity of ethanol extract of Ziziphus jujuba Mill var. spinosa seeds (Semen Ziziphi Spinosae, SZS) was investigated by behavioral tests, such as a forced swimming test (FST), a tail-suspension test (TST), and an open field test (OFT), using mice exposed to chronic unpredictable mild stress (CUMS). In the TST, immobility times of the extract-treated groups E100 and E300 (CUMS + 100 and 300 mg/kg extract, respectively) were significantly decreased in a dose-dependent manner compared with the negative control (CUMS; p < 0.01, though those of E100 and E300 were higher than those of the positive control (CUMS + 15 mg/kg fluoxetine). In the FST, immobility times of E100 and E300 were decreased compared to the normal control. In the OFT, total and zone distances of E100 and E300 were significantly higher than those of negative controls (p < 0.01) with a dose dependency. In liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis after behavioral tests, norepinephrine (NE) and 5-hydroxytryptamine (5-HT) levels in the hippocampus tissues of E100 and E300 were significantly higher than those of negative controls. Brain-derived neurotrophic factor (BDNF) levels in the hippocampus tissues of E100 and E300 were higher than those of negative controls. From these results, the SZS ethanol extract exhibited significant antidepressant-like effects via immobility decrease, distance increase, hippocampal NE and 5-HT increase, and BDNF expression. These results suggest that the extract could be a potential antidepressant agent.

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