scholarly journals Utilizing of 96% Etanol Extract Activity Red Leaf (Syzygium myrtifolium Walp.) Maseration and Socletation Method as an Antidiarrhea at Male Mice

2019 ◽  
Vol 8 (2S7) ◽  
pp. 260-263
Keyword(s):  
Effective Dose ◽  
Ethanol Extract ◽  
Extraction Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Traditional Medicines ◽  
Intestinal Length ◽  
Child Morbidity ◽  
Ethanol Extracts

Diarrhea is still one of the most important public health problems because it is the third major contributor to child morbidity and mortality in Indonesia. One of the traditional medicines that can be used to treat diarrhea is red shoots. Red shoot leaves contain secondary metabolites such as alkaloids, flavonoids, chalcones, tannins, and terpenoids which can be used as antidiarrheals. The purpose of this study was to compare the antidiare effects between extracts of maceration and socletation of red shoot leaves and determine the effective dose of both extracts as antidiarrheal in male mice using the intestinal transit method. The treatment in this study consisted of the administration of ethanol extract of shoots of red shoots by maceration at a dose of 1.58 mg / 20 g BW (dose of MI), 4.75 mg / 20g BW (dose of M.II), 6.33 mg / 20g BB (Dosage M.III) and ethanol extract of shoots of red shoots by socletation at a dose of 1.67 mg / 20 g BB (SI dose), 5.04 mg / 20 g BB (Dosage S.II), 6.72 mg / 20 g BB (Dose S.III), Loperamid HCl as positive control, and 1% CMC suspension as negative control. Observations were made by measuring the ratio of intestinal length traveled through the norit (marker) to the total gut length. The results showed that all dose treatments on maceration and socletation ethanol extracts had antidiareal activity. Different extraction methods give the same effect in giving antidiarrheal effects. Based on Duncan's advanced dose of 6.72 mg / 20 g BW of socletation results is an effective dose as antidiarrheal because it has an effect that is not significantly different than Loperamid HCl.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

scholarly journals Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

2020 ◽  
Vol 3 (2) ◽  
pp. 36-40
Author(s):  
Viki Ayu Intan Permatasari ◽  
Mutia Hariani Nurjanah ◽  
Wimbuh Tri Widodo
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
Traditional Medicines ◽  
Alternative Ingredients ◽  
High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

scholarly journals Aktivitas Ekstrak Etanol Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Penyembuhan Luka Sayat pada Tikus Putih (Rattus Norvegicus L.)

2018 ◽  
Vol 4 (2) ◽  
pp. 113-118
Author(s):  
Umi Calsum ◽  
Akhmad Khumaidi ◽  
Khildah Khaerati
Keyword(s):  
Effective Dose ◽  
Rattus Norvegicus ◽  
Povidone Iodine ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Healing Time ◽  
White Rats ◽  
Lannea Coromandelica ◽  
Healing Wound

Jawa bark (Lannea coromandelica) is a part of the plant that can be used empirically for handling wounds. This study aims to determine the activity of ethanol extract of Jawa bark on healing wound and determine the effective dose compared with povidone iodine. The test animals used were white rats (Rattus norvegicus L.) consisting of 5 groups, namely negative control (vaseline without extract), positive control (povidone iodine), dose of 250 mg/kg BW, dose of 500 mg/kg BW, dose of 750 mg/kg BW. Each rat made an incision in the area parallel to the spine with 2 cm long and 2 mm deep. Measuring the length of the wound was done every day for 14 days. The data obtained were analyzed statistically using One Way Anova and followed by Post Hoc Duncan test. Statistical results showed that the ethanol extract of Jawa bark has an activity in curing slice where the effective dose is a dose of 500 mg/kg BW with a wound healing time of 12 days.

UJI POTENSI EKSTRAK DAUN BENALU BATU (BEGONIA SP) TERHADAP KADAR GLUKOSA DALAM DARAH DAN GAMBARAN HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Glucose Levels ◽  
White Rats ◽  
Group I ◽  
Ethanol Extracts

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.

scholarly journals ACTIVITY OF 96% ETHANOL EXTRACT OF CHRYSOPHYLLUM CAINITO L. IN INCREASING VERTEBRAE TRABECULAR OSTEOBLAST CELL NUMBER IN MALE MICE

2019 ◽  
Vol 12 (1) ◽  
pp. 286
Author(s):  
Burhan Ma'arif ◽  
Agnis Pondinekaria Aditama
Keyword(s):  
Effective Dose ◽  
Ethanol Extract ◽  
Optical Microscope ◽  
Cell Number ◽  
Positive Control ◽  
Osteoblast Cell ◽  
Male Mice ◽  
Chrysophyllum Cainito ◽  
Zoom In ◽  
Hematoxylin Eosin

Objectives: This study was conducted to analyze the anti-osteoporosis effect of 96% ethanol extract from Chrysophyllum cainito L. leaves, which suspected to contain phytoestrogens, in increasing the osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice.Materials and Methods: The 96% ethanol extract of C. cainito leaves was given to male mice with dose of 2, 4, 8, and 16 mg/20 g BB mice/day after being induced orally with dexamethasone dose of 0.0029 mg/20 g BB mice/day. The positive control used was 0.026 ml/20 g BB mice/day alendronate. After 4 weeks, the increasing of osteoblast cell number in trabecular vertebrae bone of male mice was observed using an optical microscope with 100× zoom in after histomorphometry and hematoxylin-eosin staining methods.Results: The result showed the significant increasing of osteoblast number in trabecular vertebrae bone of male mice in all groups after being given treatment using 96% ethanol extract of C. cainito, with an effective dose (ED50) value of 9.5 mg/20 g BB mice/day. This increase is suspected due to the phytoestrogens content, which can also act as phytotestosterone in 96% ethanol extract of C. cainito.Conclusion: This study concluded that 96% ethanol extract of C. cainito has an activity in increasing osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice, with an ED50 value of 9.5 mg/20 g BB mice/day.

scholarly journals Laxative Activity Test of Gynura procumbens (Lour.) Merr. Leave on White Male Mice Induced by Loperamide

2019 ◽  
Vol 6 (2) ◽  
pp. 137
Author(s):  
Rahimatul Uthia ◽  
Devina Prima Yolanda ◽  
Aried Eriadi ◽  
Dwi Dinni Aulia Bakhtra
Keyword(s):  
White Male ◽  
Ethanol Extract ◽  
Negative Control ◽  
Intestinal Wall ◽  
Male Mice ◽  
Direct Stimulation ◽  
Activity Test ◽  
Intestinal Peristalsis ◽  
Ethanol Extracts ◽  
Stimulation Of

Constipation is a symptom of a problematic, non-smooth and irregular defecation process. While laxative is a substance that stimulates intestinal peristalsis as a reflex of direct stimulation of the intestinal wall that can cause or facilitate defecation. This study aimed to determine the ethanol extract of the leaves of Gynura procumbens (L.) Merr. effect as a laxative in white male mice induced by loperamide. The methods used for laxative testing are defecation patterns and intestinal transit methods. The parameters observed included frequency of defecation, stool consistency, stool weight every 30 minutes for 4 hours, and the length of the activated carbon in the mice's intestine. The extract was administered orally at a dose of 75 mg/kg BW, 150 mg/kg BW and 300 mg/kg BW, as a comparison used negative control (Na CMC). From the results of the study, it was found that the administration of ethanol extracts of Gynura procumbens (Lour.) Merr. leaves at the dose of 150 mg/kg BW gave laxative activity to white male mice (P <0.05).

scholarly journals NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF CURCUMA MANGGA VAL. IN PARACETAMOL-INDUCED MALE MICE

2018 ◽  
Vol 11 (13) ◽  
pp. 126
Author(s):  
Rosita Rosita ◽  
Yuandani Yuandani ◽  
Marianne Marianne
Keyword(s):  
Creatinine Level ◽  
Cell Damage ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dependent Manner ◽  
Male Mice ◽  
Treatment Groups ◽  
Level Measurement ◽  
Nephroprotective Activity

Objective: This study aimed to evaluate nephroprotective activity of Curcuma mangga in paracetamol-induced male mice.Methods: Male mice were divided into several groups including normal control, negative, positive, and treatment groups. Treatment groups were orally administered with C. mangga extract at doses of 100, 200, and 400 mg/kg bw for 7 days. On day 7after 1 h of treatment, the mice were induced with paracetamol 1.05 g/kg bw. Serum creatinine level measurement and histopathotlogy study were performed at the end of experiment.Results: C. mangga extract was able to inhibit the increase of creatinine level and showed a significantly different from negative control (p<0.05) and did not different significantly from positive control (p>0.05). The result was supported by histopathology examination which did not show any cell damage. The nephroprotective effect of C. mangga was in a dose-dependent manner. C. mangga extract at dose of 400 mg/kg bw depicted the strongest nephroprotective effect.Conclusion: C. mangga extract was able to protect mice kidney induced by paracetamol.

scholarly journals Anti-inflammatory Activity of Ethanol Extract from Malacca Leaves (Phyllanthus emblica) in Carrageenan Induced Male Mice

2020 ◽  
Vol 151 ◽  
pp. 01066
Author(s):  
Nuzul Asmilia ◽  
Amalia Sutriana ◽  
Dwinna Aliza ◽  
Nurfadilla Sudril
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Phyllanthus Emblica ◽  
Male Mice ◽  
Edema Volume ◽  
Volume Measurements ◽  
Group 2

The purpose of this study was to determine the effect of the ethanol extract of malacca leaves (Phyllanthus emblica) to reduce carrageenan-induced edema in mice. This study used 25 mice which were divided into 5 groups with 5 mice per group. All the mice were induced inflammation using carrageenan at a dose of 1%. Mice in group 1 were not given piroxicam and malacca leaves extract. Mice in group 2 were treated with 20 mg of piroxicam suspension. Mice in groups 3, 4, and 5 were given ethanol extract of malacca leaves with doses of 100 mg/kg bb, 200 mg/kg bb, and 300 mg/kg bb, respectively. Edema volume measurements were performed on 5th day by collecting exudates using a syringe. Data were analyzed using one-way analysis of variance and continued with the Duncan test. The result showed that the average of edema volume in the negative control group was 0.09 ± 0.02 mL, while in the positive control group was 0.05 ± 0.03 mL. The average edema volume in groups 3, 4, dan 5 were 0.07 ± 0.01 mL, 0.06 ± 0.02 mL dan 0.07 ± 0.01 mL, respectively. It is concluded that the ethanol extract of malacca leaves (Phyllanthus emblica) can reduce the carrageenan-induced edema in male mice.

scholarly journals EFFICACY OF A STANDARDIZED ETHANOL EXTRACT OF ROSELLE CALYX IN THE TREATMENT OF ORAL MUCOSA ULCERATION (IN VIVO)

2017 ◽  
Vol 10 (17) ◽  
pp. 183
Author(s):  
Reni Jayantini ◽  
Dewi Fatma Suniarti ◽  
Agoeng T Sarwono
Keyword(s):  
Topical Application ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Ethanol Extracts ◽  
Inflammation Score

 Objectives: To analyze the effectiveness of the topical application of a standardized ethanol extract of roselle calyx in the treatment of oral mucous ulceration.Methods: Twelve Sprague Dawley rats were divided into two groups, with oral mucous ulcer being induced in all the rats. The ulcer diameter, reddish color, and the swollen membrane around the ulcer, as well as the inflammation score of the ulcer, were recorded for all animals. The negative control group was treated with aquades, while the positive control group was treated with 0.1% triamcinolone acetonide. The treatment group received the topical application of 7.5% and 15% standardized ethanol extract of roselle calyx twice a day for 3 or 7 days. On the 4th day, six rats were sacrificed, while the remaining six rats continued to receive treatment as before. On the 8th day, all the rats were sacrificed. Specimens were fixed, and histology slides were made. Further, microscopic slides were observed and scored.Result: Both the 7.5% and 15% standardized ethanol extracts of roselle calyx were found to reduce the ulcer diameter and inflammation score. No swelling or redness was observed.Conclusions: The application of 7.5% standardized ethanol extract of roselle calyx for 7 days is equally as effective as the application of 0.1% triamcinolone acetonides, except in terms of the decrease in the inflammation score.

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