BIOCHEMICAL STUDY OF CERTAIN ENZYMES IN DENGUE FEVER PATIENTS

2020 ◽  
pp. 72-73
Author(s):  
JAIN SUMAN ◽  
SAMAR RAJENDRAR KUMAR ◽  
SOGANI SONAL
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Dengue Fever ◽  
Blood Levels ◽  
Serum Glutamic Pyruvic Transaminase ◽  
Pyrimidine Nucleotides ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Reactive Protein ◽  
Purine And Pyrimidine Nucleotides ◽  
New Protein

Objective: The main objective of the present study is to correlate the effect of certain enzymes in diagnosis of dengue fever (DF) and impact of these elevated/decreased blood levels of enzymes on patients of DF. Methods: The study was carried out on 30 patients suspected to be suffering from DF. Blood sample was collected and tested for various parameters such as protein (albumin), lactate dehydrogenase (LDH), creatinine kinase (CK), C-reactive protein (CRP), acid phosphatase, alkaline phosphatase, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT). Results: Serum enzymes such as LDH and CK were found to be elevated to be a good marker of muscle damage and progression of diseases. CRP as a marker of inflammation was also found to be elevated. Enzymes like acid phosphatase increased abruptly while alkaline phosphatase was irregular. Liver enzymes SGOT and SGPT activity were also enhanced. Conclusion: Increased level of transaminases can be reflective of the formation of various amino acids for the synthesis of new protein and for channeling of glutamate and aspartate along with glycine for the synthesis of purine and pyrimidine nucleotides. Objective: The main objective of the present study is to correlate the effect of certain enzymes in diagnosis of dengue fever (DF) and impact of these elevated/decreased blood levels of enzymes on patients of DF. Methods: The study was carried out on 30 patients suspected to be suffering from DF. Blood sample was collected and tested for various parameters such as protein (albumin), lactate dehydrogenase (LDH), creatinine kinase (CK), C-reactive protein (CRP), acid phosphatase, alkaline phosphatase, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT). Results: Serum enzymes such as LDH and CK were found to be elevated to be a good marker of muscle damage and progression of diseases. CRP as a marker of inflammation was also found to be elevated. Enzymes like acid phosphatase increased abruptly while alkaline phosphatase was irregular. Liver enzymes SGOT and SGPT activity were also enhanced. Conclusion: Increased level of transaminases can be reflective of the formation of various amino acids for the synthesis of new protein and for channeling of glutamate and aspartate along with glycine for the synthesis of purine and pyrimidine nucleotides.

scholarly journals Usefulness of C-Reactive Protein in Differentiating Acute Leptospirosis and Dengue Fever in French Guiana

2019 ◽  
Vol 6 (9) ◽  
Author(s):  
Paul Le Turnier ◽  
Timothée Bonifay ◽  
Emilie Mosnier ◽  
Roxane Schaub ◽  
Anne Jolivet ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Antibiotic Therapy ◽  
Dengue Fever ◽  
Bivariate Analysis ◽  
Blood Levels ◽  
Elevated Blood ◽  
C Reactive Protein ◽  
Positive Serology ◽  
Reactive Protein ◽  
Ns1 Antigen

Abstract Objective Leptospirosis and dengue fever (DF) are hard-to-differentiate diseases in cocirculating areas, especially during DF epidemics. Misdiagnosis and ensuing lack of antibiotic therapy can be detrimental in leptospirosis. The objective of this study was to identify factors that help differentiate acute leptospirosis from dengue fever on admission. Method Patients with leptospirosis (positive serology or polymerase chain reaction) were compared with patients with DF (positive nonstructural 1 [NS1] antigen) in a case-control study with age matching. Data on admission were compared using bivariate analysis and multivariate analysis. Results Seventy-two patients with leptospirosis were compared to 216 patients with DF. In bivariate analysis, the factors associated with leptospirosis were male gender, cough, anemia, and elevated blood levels of C-reactive protein (CRP), leukocytes, creatinine, bilirubin, and creatine phosphokinase. Exanthema, purpura, myalgia, headache, and neutropenia were associated with DF. In multivariate analysis, elevated blood levels of leukocytes, bilirubin, and CRP were associated with leptospirosis. The CRP threshold of 50 mg/L taken alone had elevated sensitivity and specificity. Conclusions The CRP level, an easy-to-obtain biomarker, was a powerful tool to differentiate on admission leptospirosis and DF. Facing a dengue-like syndrome in cocirculating areas and awaiting new specific rapid diagnostic tests, CRP dosing could help the clinician to promptly consider the diagnosis of leptospirosis and initiate antibiotic therapy early.

scholarly journals Epidemiological Study of Dengue Hemorrhagic Fever (DHF) With Increased Incidence of Serum Glutamic Pyruvic Transaminase (SGPT) Levels at Aura Syifa Hospital Kediri

2021 ◽  
Vol 4 (1) ◽  
pp. 54-58
Author(s):  
Mia Ashari Kurniasari ◽  
Anggit Saputri Okta Nurziah
Keyword(s):  
Dengue Fever ◽  
Epidemiological Study ◽  
Dengue Hemorrhagic Fever ◽  
Sampling Technique ◽  
Hemorrhagic Fever ◽  
Severe Bleeding ◽  
Glutamic Pyruvic Transaminase ◽  
Serum Glutamic Pyruvic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Descriptive Research

Dengue fever virus is an infectious disease that can infect others if bitten by Aedes aegypti mosquito. The virus has the potential to attack cells in the hepar organs so that the hepar is inflamed, swollen, and liver function is disrupted, and there is severe bleeding. The increase in transaminase enzymes as well as hepatomegaly is a frequent sign in dbd sufferers, thus reinforcing the notion that the course of dengue hemorrhagic fever (DHF) disease can affect hepar or liver organs. The presence of such events will increase the level of Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT) in dengue fever patients. The purpose of this study is to find out the epidemiological study of Dengue dengue fever with the increase in SGPT and SGOT levels in dengue fever patients at Aura Syifa Kediri Hospital. This research method uses descriptive research design and total sampling technique for 1 month. Samples obtained for 1 month amounted to 15 samples. The results showed that from a sample of 15 respondents as many as 3 samples (20%) for high SGPT levels above normal and normal results as many as 12 samples (80%). The conclusion of this study shows that the value of SGPT levels has increased by 20% and is still above normal, but still have to be alert to small values.

Effects of Kanwa on Rat Gastrointestinal Phosphatases

2010 ◽  
Vol 3 (3) ◽  
pp. 1147-1152
Author(s):  
Jacob Bamaiyi ◽  
Omajali ◽  
Sanni Momoh
Keyword(s):  
Alkaline Phosphatase ◽  
Small Intestine ◽  
Acid Phosphatase ◽  
Sodium Carbonate ◽  
Elevated Level ◽  
Food Additive ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases ◽  
Diagnostic Indices ◽  
High Level

This study investigates the effects of kanwa on rat gastrointestinal phosphatases. The rats were administered 7% w/v concentration of  trona (Kanwa) orally for a period of two weeks in order to investigate how this compound is being used as food additive in some homes in Nigeria. The Kanwa used in this study was the handpicked variety obtained from sellers from Anyigba market in eastern part of Kogi State, Nigeria. Kanwa, a hydrated sodium carbonate (Na2CO3NaHCO3.2H2O) was obtained as a dried lake salt. Acid phosphatase has the ability to dephosphorylate molecules containing phosphate group. The decreased and elevated level in serum or plasma acid and alkaline phosphatases serves as diagnostic indices for various diseases. Results showed that there was increase and decrease of acid phosphatase (ACP) activities in both the stomach and small intestine. The activities of alkaline phosphatase (ALP) fluctuated in the small intestine. However, in the stomach, an increase activity of ALP was noticed throughout the period of ‘Kanwa’ administration. We concluded that although the level of ‘Kanwa’ consumed in most homes may not be toxic if not taken continuously or repeatedly. Thus, continuous consumption should be discouraged as accumulation of high level of ‘Kanwa’ may cause damages or injuries to the various organs/tissues and may disrupt normal body function.

scholarly journals Bone-Derived Serum Enzymes and Bone Density in Perimenopausal Caucasian Women

1993 ◽  
Vol 30 (2) ◽  
pp. 191-194 ◽  
Author(s):  
J D Johnston ◽  
S Koneru ◽  
T Kuwana ◽  
S B Rosalki
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Bone Density ◽  
Femoral Neck ◽  
Serum Levels ◽  
Bone Alkaline Phosphatase ◽  
Serum Enzymes ◽  
Tartrate Resistant Acid Phosphatase ◽  
Vertebral Bone ◽  
Caucasian Women

Serum levels of bone-origin alkaline phosphatase and of tartrate-resistant acid phosphatase were measured in Caucasian women aged 41–69 years who had volunteered for bone densitometry. Bone alkaline phosphatase and tartrate-resistant acid phosphatase were inversely correlated with vertebral bone density and with femoral neck bone density. Bone alkaline phosphatase and acid phosphatase were also significantly correlated, consistent with the concept of ‘coupling’ between osteoblast and osteoclast activity.

scholarly journals Phosphatase synthesis in Klebsiella (Aerobacter) aerogenes growing in continuous culture

1972 ◽  
Vol 127 (1) ◽  
pp. 87-96 ◽  
Author(s):  
P. G. Bolton ◽  
A. C. R. Dean
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Continuous Culture ◽  
Activity Profile ◽  
Glucose Effect ◽  
Ph Values ◽  
Nitrophenyl Phosphate ◽  
Wide Range ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of

1. Phosphatase synthesis was studied in Klebsiella aerogenes grown in a wide range of continuous-culture systems. 2. Maximum acid phosphatase synthesis was associated with nutrient-limited, particularly carbohydrate-limited, growth at a relatively low rate, glucose-limited cells exhibiting the highest activity. Compared with glucose as the carbon-limiting growth material, other sugars not only altered the activity but also changed the pH–activity profile of the enzyme(s). 3. The affinity of the acid phosphatase in glucose-limited cells towards p-nitrophenyl phosphate (Km 0.25–0.43mm) was similar to that of staphylococcal acid phosphatase but was ten times greater than that of the Escherichia coli enzyme. 4. PO43−-limitation derepressed alkaline phosphatase synthesis but the amounts of activity were largely independent of the carbon source used for growth. 5. The enzymes were further differentiated by the effect of adding inhibitors (F−, PO43−) and sugars to the reaction mixture during the assays. In particular, it was shown that adding glucose, but not other sugars, stimulated the rate of hydrolysis of p-nitrophenyl phosphate by the acid phosphatase in carbohydrate-limited cells at low pH values (<4.6) but inhibited it at high pH values (>4.6). Alkaline phosphatase activity was unaffected. 6. The function of phosphatases in general is discussed and possible mechanisms for the glucose effect are outlined.

SIGNIFICANCE OF TRANSAMINASE ACTIVITY OF SERUM

PEDIATRICS ◽  
1959 ◽  
Vol 24 (3) ◽  
pp. 360-361
Author(s):  
SAMUEL P. BESSMAN
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Transaminase Activity ◽  
Specific Approach ◽  
Normal Tissues ◽  
The Past ◽  
Glycolytic Activity ◽  
Enzyme Characteristic ◽  
Phosphatase Alkaline

THE MEASUREMENT of enzyme activity of serum as an indicator of disease has a long history in medicine. In the past, it has been the aim of the designers of these methods to make them as specific as possible for assay of an enzyme characteristic of a particular system or group of similar organs. Examples of these venerable tests are those for amylase, acid phosphatase, alkaline phosphatase and choline esterase in the serum. Warburg made the first departure from this specificity by demonstrating that the activity of triosephosphate dehydrogenase in the serum of animals with cancer was much greater than that of controls. This test was partially specific, for as Warburg had earlier shown, the glycolytic activity of tumors is much greater than that of normal tissues. The non-specific approach became extreme with the introduction of the measurement of the glutamic-oxalacetic transaminase reaction in the diagnosis of acute coronary disease.

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