scholarly journals IN VITRO ANTIBACTERIAL EFFECT OF BREADFRUIT LEAF EXTRACT AGAINST STREPTOCOCCUS SANGUINIS ATCC 10556 BIOFILM

2019 ◽  
pp. 92-95
Author(s):  
VAZA NADIA ◽  
SRI UTAMI ◽  
RATNA FARIDA
Keyword(s):  
Leaf Extract ◽  
Antibacterial Properties ◽  
Control Group ◽  
Streptococcus Sanguinis ◽  
Diphenyltetrazolium Bromide ◽  
Objective Evidence ◽  
Accumulation Phase ◽  
Bacterial Plaque ◽  
Insight Into

Objective: Evidence indicates that breadfruit leaf extract may have antibacterial properties. In terms of bacterial plaque, Streptococcus sanguinis isknown as an early agent of its formation. The purpose of this study was to analyze the antibacterial effects of breadfruit leaf extract on the growth ofS. sanguinis.Methods: S. sanguinis ATCC 10556 was cultured in 96-well plates and was incubated at 37°C for 20 h (the accumulation phase) or 24 h (the maturationphase). Then, breadfruit leaf extract was added at concentrations of 5, 10, 20, 40, 80, and 100%. The viability of S. sanguinis was evaluated using the3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay at a wavelength of 490 nm.Results: Our results demonstrated that the viability of S. sanguinis, after exposure to the breadfruit leaf extract at all concentrations, during theaccumulation and maturation phases was lower than the control group (p<0.05). In addition, the viability of S. sanguinis after exposure to the breadfruitleaf extract at concentrations of 20, 80, and 100% during the accumulation phase was lower than that observed during the maturation phase.Conclusion: Collectively, our novel findings should provide insight into the potential of breadfruit leaf extract to positively affect oral and dentalhealth.

scholarly journals EFFECT OF BREADFRUIT LEAF EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS BIOFILM IN VITRO

2019 ◽  
pp. 96-99
Author(s):  
SEPTI WARDA ZULFIKAR ◽  
SRI UTAMI ◽  
RATNA FARIDA
Keyword(s):  
Oral Cavity ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
The Other ◽  
Control Group ◽  
Maturation Phase ◽  
Accumulation Phase

Objective: Breadfruit leaf has potent antibacterial properties that could be used to reduce biofilms in the oral cavity. The purpose of this study was toanalyze the antibacterial effect of the breadfruit leaf extract on the growth of Streptococcus mutans in vitro.Methods: S. mutans ATCC 25175 was cultured in a 96-well plate and incubated at 37°C for 20 h (accumulation phase) and 24 h (maturation phase).The breadfruit leaf extract was added at the following concentrations: 5%, 10%, 15%, 20%, 40%, 80%, and 100%. The viability of S. mutans was testedwith the MTT assay at a wavelength of 490 nm. The results were analyzed by one-way analysis of variance.Results: In the accumulation phase, a significant decrease was found in S. mutans viability at different concentrations of the breadfruit leaf extract, butin the maturation phase, a significant decrease was found in the S. mutans viability at the 5% concentration. The other groups decreased significantlycompared with the control group (*p<0.05). The viability of S. mutans after adding the breadfruit leaf extract at all concentrations was lower in theaccumulation phase than that in the maturation phase.Conclusion: In the accumulation phase, breadfruit leaf extract at concentrations of 5%, 10%, 20%, 40%, 80%, and 100% can reduce S. mutans biofilmviability.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK CATHECHIN TEH PUTIH TERHADAP STREPTOCOCCUS SANGUINIS

2017 ◽  
Vol 4 (2) ◽  
pp. 108
Author(s):  
Muhamad Zakki
Keyword(s):  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Test Material ◽  
Minimal Bactericidal Concentration ◽  
In Vitro Tests ◽  
Streptococcus Sanguinis ◽  
White Tea ◽  
Bacterial Plaque

Background: Streptococcus sanguinis is known as an early agent of formation of bacterial plaque. White tea is one of plant with various biological activities that are beneficial to human health.Objective: to examine the antibacterial activity of white tea cathechin extract against bacteria S. sanguinis.Methods: test material was white tea cathechin extract. In vitro tests were conducted to obtain minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).Result: The MIC of White tea cathechin extract was 500ppm (0,5 mg/ml) and MBC was 2000ppm (2mg/ml).Conclusion: White tea cathechin extract has antibacterial properties. White tea cathechin extract can be used as herbal moutwash to control dental plaque.

scholarly journals THE INHIBITORY ACTIVITY OF KELAKAI LEAF EXTRACT AGAINST THE GROWTH OF Porphyromonas gingivalis ATCC® 33277™

2020 ◽  
Vol 5 (2) ◽  
pp. 104
Author(s):  
Destri Khusnul Khotimah ◽  
I Wayan Arya Krishnawan Firdaus ◽  
Maharani Laillyza Apriasari
Keyword(s):  
Porphyromonas Gingivalis ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Bacterial Species ◽  
Diffusion Method ◽  
Control Group ◽  
Inhibitory Zone ◽  
Mueller Hinton Agar ◽  
Inhibitory Power

ABSTRACTBackground: Chronic periodontitis is an infectious disease that causes damage on periodontal ligament and alveolar bone. The severity of periodontitis is caused by several types of bacterial species which one of them is Porphyromonas gingivalis bacteria with a prevalence of 85% in oral cavity. The extract of kelakai leaf contained antibacterial in the form of flavonoid, alkaloid, tannin, and steroid. Flavonoid consists of some chemical compounds which is one of them is quercetin. The level of quercetin in kelakai leaf is 503.56 mgQE/g. From some secondary metabolites, kelakai leaf has inhibitory power toward gram negative bacterial, Porphyromonas gingivalis. Objective: This research was intended to know the activity of inhibitory power of kelakai leaf toward Porphyromonas gingivalis bacteria. Method: This research was an experimental research consisted of 5 experimental groups that were group of kelakai leaf extract on the concentrations of 100 mh/ml, 75 mg/ml, 50mg/ml, and 25 mg/ml and the control group (0.2% chlorhexidine). Each treatment was done in 4 repetitions. The test of inhibitory power used diffusion method by measuring the inhibitory zone around the growth of Porphyromonas gingivalis on Mueller Hinton Agar media. The data were analyzed by using One Way Anova 95% and then continued with LSD. Results: Based on the LSD test, it was known that the extract of Kelakai leaf had inhibitor power activity toward Porphyromonas gingivalis. The highest inhibitory zone was on the concentration of 100 mg/ml with inhibitory zone of 14.61 mm. Conclusion: The extract of kelakai leaf had inhibitory power activity toward Porphyromonas gingivalis bacteria in vitro. Keywords: 0.2% chlorhexidine, Diffusion method, Inhibitory power, Stenochlaena palustris extract, Porphyromonas gingivalis.

scholarly journals ANTIBACTERIAL EFFECTIVITY OF FLAVONOID FRACTION OF RAMANIA LEAF EXTRACT (Bouea macrophylla Griffith) AGAINST Porphyromonas gingivalis

2021 ◽  
Vol 6 (1) ◽  
pp. 72
Author(s):  
Lailatul Qomariyah ◽  
Fransiska Uli Arta Panjaitan
Keyword(s):  
Periodontal Disease ◽  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Chlorhexidine Gluconate ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Flavonoid Fraction

ABSTRACTBackground: Chronic periodontitis is a periodontal disease with 80% of all cases of periodontitis. The major causes are the accumulation of plaque and bacteria. The dominant bacteria in chronic periodontitis is Porphyromonas gingivalis. Treatment of chronic periodontitis can be done by scaling and root planing and supporting therapy by using mouthwash such as Chlorhexidine gluconate 0.2% which is the gold standard in the treatment of periodontal disease. Chlorhexidine gluconate 0.2% has disadvantages so that nowadays research on herbal plants is being done to find alternative medicines that are more effective. Ramania (Bouea machropylla Griffith) leaf contains flavonoids that have antibacterial properties. Objective: To analyze the antibacterial effectivity of the flavonoid fraction of Ramania leaf extract against Porphyromonas gingivalis that causes chronic periodontitis. Method: True experimental study and post-test with control group design consisting of 5 treatment groups, namely flavonoid fraction of ramania leaf extract with concentrations of 0.1%, 0.3%, and 0.5%, chlorhexidine gluconate 0.2% as a control positive and aquadest as a negative control. Each group was repeated 6 times. Antibacterial tests using the dilution method with inhibitory rates calculated using a UV-Vis spectrophotometer and killing rates were calculated using a Colony Counter. Results: The average difference in absorbance values obtained inhibitory rates at concentrations of 0.1%, 0.3%, and 0.5%. One Way Anova Test showed a significance value of 0,000 (p < 0.05). The average number of colonies after 24 hours incubation showed the results of a kill rates in the concentration group of 0.3%, 0.5%, and positive control. The Kruskal Wallis test showed a significance value of 0,000 (p < 0.05). Conclusion: The minimum inhibitory concentration (MIC) was obtained at a concentration of 0.1% and the minimum bactericidal concentration (MBC) was obtained at 0.3% concentration.Keywords: Flavonoid Fraction, MBC, MIC, Porphyromonas Gingivalis, Ramania Leaf Extract.

EFFECTIVENESS TEST OF ANTIBACTERIAL EXTRACT MINT LEAF (Mentha piperita L.) INHIBIT GROWTH IN Streptococcus sanguinis

2021 ◽  
Vol 1 (Volume 1 No 1) ◽  
pp. 49-59
Author(s):  
Herryawan ◽  
Rahmadaniah Khaerunnisa ◽  
Fadhilah Nuraini Fajri
Keyword(s):  
Leaf Extract ◽  
Large Diameter ◽  
Test Group ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Control Group ◽  
Oral Diseases ◽  
Streptococcus Sanguinis ◽  
Study Results ◽  
Mint Leaf

Streptococcus sanguinis (s.sanguinis) is one of the normal flora in the oral cavity. These bacteria act as a pioneer in forming plaques that cause most dental and oral diseases. It takes an antibacterial agent to be able to inhibit the clips of the plaque. One of the herbal plants that have been known to have antibacterial power is the mint leaf (Mentha Piperita L.). The antibacterial potency of this plant comes from its chemical compound content, such as flavonoids, polyphenols, tannins, and menthols. This study aimed to determine the inhibitory of mint leaf extract on the growth of Sanguinis. An experimental laboratory carries out research. Tests were carried out using the diffusion method in four test groups, namely mint leaf extract with a concentration of 2.5%, 5%, 7.5%, and 10%, and one control group that uses DMSO. Each treatment group repeated five times on the Mueller-Hinton media (MHA) using disk paper and incubated for 24 hours. The inhibition zone can be seen in the transparent area formed around the disk paperand then measured using the Sorong term to find a large diameter. Statistical analysis was carried out using the Kruskal-Wallis test. The study results that the largest inhibitory zone diameter was obtained in a test group with a concentration of 10% (16.39 mm), while the lowest diameter in the control group (4.89 mm) 10% concentration was the highest among all test groups. The DMSO did not have an antibacterial effect. The higher the concentration of an antibacterial compound, it will also increase its effectiveness.

scholarly journals The role of beluntas (pluchea indica less.) leaf extract in preventing the occurrence of fibroblasts hyperproliferation: An in vitro preliminary study

2019 ◽  
Author(s):  
Sakti Charlia Maharani ◽  
Indah Julianto ◽  
Suci Widhiati
Keyword(s):  
Leaf Extract ◽  
In Vitro Study ◽  
Collagen I ◽  
Control Group ◽  
Antibody Treatment ◽  
Average Decrease ◽  
Matrix Deposition ◽  
Post Hoc

Beluntas (Pluchea indica Less.) is a herbal plant which contains variety of benefits. Quercetin, one of flavonoid, is the most bioactive agent in beluntas leaf. Collagen inhibition by quercetin may modulate extracellular matrix deposition and inhibit the formation of hypertrophic scar. This was an in vitro study with senescent fibroblasts to determine the role of beluntas leaf extract in preventing the occurence of fibroblasts hyperproliferations. There were 4 groups were stained by anti-collagen I antibodies and secondary antibody. Flowcytometry analysis was done to measure the fibroblasts density. Anova test was performed with a value of p=0.000 (p<0.05). A post hoc analysis showed significant differences in the average decrease of fibroblasts that absorbs staining anti-collagen I antibody treatment group compared with the control group. There were significant effects of beluntas leaf extract in preventing the occurrence of fibroblasts hyperproliferations. Beluntas leaf extract with a concentration of 80 mol/L had the most significant effect on the fibroblasts density. Thus beluntas leaf extract has the ability in preventing the occurrence of fibroblasts hyperproliferation.

scholarly journals THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO

2018 ◽  
Vol 11 (12) ◽  
pp. 411
Author(s):  
Ameta Primasari ◽  
Minasari Nasution ◽  
Nurul Hidayati Arbi ◽  
Dini Permata Sari ◽  
Mohammad Basyuni
Keyword(s):  
Leaf Extract ◽  
Brain Heart Infusion ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Negative Control ◽  
Plate Count ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Mueller Hinton Agar

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.

Effect of Titanium Nanoparticles on Osteoblast Proliferation

2020 ◽  
Vol 12 (4) ◽  
pp. 455-460
Author(s):  
Yuan Wu ◽  
Cuizhong Liu ◽  
Ming Gao ◽  
Qiang Liang ◽  
Yu Jiang
Keyword(s):  
Histochemical Staining ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Osteogenic Activity ◽  
Diphenyltetrazolium Bromide ◽  
Proliferation And Differentiation ◽  
Titanium Nanoparticles

This study aimed to observe the effect of titanium nanomaterials on osteoblastsin vitro. Osteoblasts were identified using histochemical staining, and they were examined using an MTT (3-(4,5Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay to determine the proliferation and differentiation of osteoblasts. In addition, we observed the effect of titanium nanomaterials on the function of osteoblasts. Compared with the control group, titanium nanomaterials promoted the growth, proliferation, and differentiation of osteoblasts. Our findings showed that titanium nanomaterials can significantly promote the proliferation of osteoblasts and enhance their osteogenic activity.

scholarly journals Effectivity of Jatropha multifida L. Leaves Extract as Antibacterial on Streptococcus mutans using In Vitro Testing Methods

2021 ◽  
Vol 7 (2) ◽  
pp. 415-421
Author(s):  
Listyaning Kartiko Kinasih ◽  
Idamawati Nababan ◽  
Suci Erawati ◽  
Rouli Natasia M Simanjuntak
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Control Group ◽  
Entire Body ◽  
Body Parts ◽  
Control Group Design ◽  
The Individual ◽  
Jatropha Multifida

Intoduction: Health is the condition of the entire body and body parts of an individual who is free from disease both physically, mentally and socially, thus enabling the individual to be able to carry out daily activities more productively. However, many people neglect their own health, especially in the field of dental and oral health. Caries is one of the most common oral and dental problems. The prevalence of dental caries in Indonesia is high, namely 88.8%. Dental caries is caused by Streptococcus mutans bacteria which plays a role in the conversion of sucrose to lactic acid. Infection due to Streptococcus mutans bacteria can be treated by taking antibiotics. Apart from drugs, antibiotics can also be obtained from natural ingredients, one of which is the tintir castor plant (Jatropha multifida Linn) or commonly known as the betadine. Objective: To see the effect of the antibacterial content of tintir leaves against Streptococcus mutans bacteria and to determine the differences in the antibacterial effect of betadine leaf extract (Jatropha multifida L.) from a concentration of 25%, 50%, 75%, and 100% against Streptococcus mutans. Methods: The type of research used in this study was a laboratory experimental study with a post-test-only control group design as the research design and to test the sensitivity of bacteria using the paper disc diffusion test. Data analysis using One-Way ANOVA. Result: The results of this study indicate the effect of tintir castor leaf extract (Jatropha multifida L.) in inhibiting the growth of Streptococcus mutans bacteria.

scholarly journals ANTIBACTERIAL EFFECTIVITY OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus sanguinis BACTERIA

2020 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Dita Puspita Sari ◽  
Didit Aspriyanto ◽  
Irham Taufiqurrahman
Keyword(s):  
Leaf Extract ◽  
Periodontal Diseases ◽  
Tooth Surface ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Streptococcus Sanguinis ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.

Sign in / Sign up

Export Citation Format

Share Document