scholarly journals ANTIDIABETIC EFFECT OF EXTRACTS OF BLUMEA LACERA DC. IN STREPTOZOTOCIN INDUCED HYPERGLYCEMIC RATS

2017 ◽  
Vol 9 (10) ◽  
pp. 218
Author(s):  
Diptirani Rath ◽  
Snigdha Rani Panigrahy ◽  
Sandeep Kumar Panigrahi ◽  
Durga Madhab Kar ◽  
Laxmidhar Maharana
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Methanol Extract ◽  
Glycated Haemoglobin ◽  
Tolerance Test ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Standard Drug ◽  
Liver And Pancreas ◽  
Blumea Lacera

Objective: Present study of this research was undertaken to evaluate the antidiabetic activities of extracts of aerial parts of Blumea lacera DC. (Asteraceae) in streptozotocin (STZ) induced hyperglycemic rat.Methods: The methanol extracts (MEBL) and aqueous extract (AEBL) of B. lacera DC. were investigated in streptozotocin (STZ) induced hyperglycemic rats at a dose level of 200 and 400 mg/kg body weight, in oral glucose tolerance test (OGTT), acute and subacute antidiabetic (30 d) models keeping a parallel group of metformin (250 mg/kg body weight) as standard drug. The serum biochemical parameters, histopathology of liver and pancreaswere examined and analyzed statistically.Results: Treatment with methanol extract of B. lacera (MEBL) at a dose of 200 mg/kg and 400 mg/kg body weight, significantly decrease (p<0.05) blood glucose level from 289.83±9.83 and 289.83±2.71 to 201.83±8.87 and 105.00±2.05 respectively with corresponding percentage fall of blood glucose to 30.40±1.79 and 63.78±0.59. It also improved the glycated haemoglobin (HbA1c) near to normal value, restored the lipid and bio-chemical level and rejuvenate beta cells of pancreas, thereby improve insulin secretion.Conclusion: The result of the present study concluded that extracts of B. lacera DC possess marked antidiabetic activity. However, methanol extract of B. lacera (MEBL) at the dose of 400 mg/kg showed significant dose dependent antidiabetic effects without any destruction and restores the structure of liver and pancreas of hyperglycemic rats.

Antidiabetic Effects of Ethanolic Extract of Ficus glomerata (L.) Roots

2020 ◽  
Vol 16 (1) ◽  
pp. 33-41
Author(s):  
Mohini C. Upadhye ◽  
Uday Deokate ◽  
Rohini Pujari ◽  
Vishnu Thakare
Keyword(s):  
Body Weight ◽  
Glucose Tolerance ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Oral Glucose ◽  
Ficus Glomerata

Background: Ficus glomerata (F. glomerata) Linn. Family Moraceace is a large tree found all over India including outer Himalayan ranges, Punjab, Chota Nagpur, Bihar, Orissa, West Bengal, Rajasthan, Deccan and also as a common plant in South India. It is planted around the home and temples. It is cultivated throughout the year, distributed in evergreen forests and moist localities. Objective: The Ethanolic Extract of roots of F. Glomerata (EEFG) belonging to the family Moraceace, was investigated for its antidiabetic activity using alloxan induced diabetic rats. Methods: Thirty rats were divided into 5 groups having 6 rats in each group. The alloxan was administered to the rats of all groups except normal control group through intraperitoneal route at a concentration of 140mg/kg body weight. A dose of 100mg/kg and 200 mg/kg body weight of EEFG was administered to alloxan induced diabetic rats. The administration of the extract was lasted for 11 days. Effectiveness of the extract on glucose, cholesterol, triglycerides, and high density lipoprotein and protein concentrations was analyzed. Results: Significant (p<0.05) reduction in the levels of glucose, cholesterol, triglyceride of the diabetic rats was observed after treatment with ethanolic extract. After subjecting to oral glucose tolerance test EEFG also showed significant improvement in glucose tolerance. Conclusion: F. glomerata root ethanolic extract showed that it possesses antidiabetic effect and can be found useful for the management of diabetes mellitus.

scholarly journals Evaluation of Bioactivities of Gouania tiliaefolia Lam., an Indigenous Traditional Medicinal Plant of Bangladesh

2019 ◽  
Vol 22 (2) ◽  
pp. 200-207
Author(s):  
Tufael Ahmed ◽  
Rifat Khan ◽  
Nafisa Tabassum ◽  
Fahima Aktar ◽  
Mohammad Kaisarul Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Analgesic Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Oral Glucose ◽  
Tail Flick ◽  
Cytotoxic Activities ◽  
Crude Methanol Extract

The crude methanol extract of Gouania tiliaefolia Lam. was partitioned by the modified Kupchan method and the fractions were evaluated for total phenolic content, antioxidant, cytotoxic, thrombolytic, hypotonic and heat-induced membrane stabilizing activities. The petroleum ether soluble fraction (PESF) and methanol extract (ME) showed the highest phenolic content of 78.30 ±1.60 mg and 70.37 ± 0.84 mg, respectively, which were expressed in gallic acid equivalent (GAE). Similar trends were observed in case of anti-oxidant and cytotoxic activities, where the PESF possessed the highest free radical scavenging activity and brine shrimp lethality (IC50 = 2.88 ± 0.02 μg/ml, LC50 = 2.59 ± 0.14 μg/ml), followed by ME (IC50 = 4.79 ± 0.17 μg/ml, LC50 = 3.38 ± 0.08 μg/ml) and CSF (IC50 = 37.51 ± 0.96 μg/ml, LC50 = 73.55 ± 0.26 μg/ml). In case of assays for thrombolytic and membrane stabilizing activities, all extractives showed insignificant results compared to the respective standards. The crude methanol extract of G. tiliaefolia was used to examine the in-vivo analgesic (central and peripheral), antidiarrheal and antidiabetic activities in Swiss albino mice. In case of castor oil induced diarrhea, the ME gave better reduction of diarrhea by 71.43% (at 400 mg/kg-body weight) compared to loperamide (64.29%). Antidiabetic activity was evaluated by oral glucose tolerance test and the ME showed 71.42% and 75.39% reduction of blood glucose at doses 200 and 400 mg/kg-body weight, respectively when compared with the standard glibenclamide that reduced blood glucose by 66.17%. The central- and peripheral-analgesic activity was evaluated by the tail-flick test and acetic acid induced writhing test, respectively. In both the cases, ME demonstrated dose-dependent analgesic activity compared to the standards. Bangladesh Pharmaceutical Journal 22(2): 200-207, 2019

scholarly journals Pharmacological potential of methanol extract of Anacardium occidentale stem bark on alloxan-induced diabetic rats

2018 ◽  
Vol 5 (7) ◽  
pp. 2440-2454
Author(s):  
D. A. Omoboyowa ◽  
F. O. Afolabi ◽  
T. C. Aribigbola
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Methanol Extract ◽  
Stem Bark ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Β Cells

Background: The anti-hyperglycemic potential of methanol stem bark extract of Anacardium occidentale (MSBEAO) was investigated using an alloxan-induced diabetic rat model. Alloxan administration induces the generation of free radicals which can affect antioxidant status resulting in the disruption of the β-cells of the pancreas. Therefore, this study examines the antioxidant potential of the plant extract and the ameliorating effect on the pancreas of alloxan-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of 150 mg/kg body weight of alloxan monohydrate. MSBEAO, at a concentration of 100 or 200 mg/kg b.w. was orally administered to alloxan-induced diabetic rats and normal rats. The hypoglycemic effect, oral glucose tolerance test, and biochemical assay of alloxan-induced diabetic rats were assayed using standard procedures. Results: Preliminary phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, terpenoids, carbohydrates, and phenols at moderate concentrations. The lethality dose (LD50) of the plant extract was found to be equal to or less than 5000 mg/kg b.w. The hypoglycemic effect of the extract on the non-diabetic rats revealed a significant (p<0.05) decrease in the blood glucose concentration of animals administered with 1 g/kg b.w. of the extract, compared to normal control rats administered with normal saline. In the oral glucose tolerance test, the methanol extract exerted the highest response, similar to glibenclamide after 15 and 30 minutes of administration, compared to the control rats. The methanol extract yielded the highest blood glucose lowering effects after 9 days of treatment (p<0.05), compared to diabetic rats administered with normal saline and 0.3 mg/kg b.w. of glibenclamide. Administration of the extract at 200 mg/kg b.w. showed improved pancreas architecture and regeneration of the β-cells, compared with the pancreas of animals in the other groups. Conclusion: The results of this study suggest that MSBEAO is a potentially effective agent for the management of diabetes which might result from the antioxidant-generating capacity of the stem bark.

Lack of effect of oral glucose loading on conduit vessel endothelial function in healthy subjects

2004 ◽  
Vol 107 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Aris SIAFARIKAS ◽  
Katie WATTS ◽  
Petra BEYE ◽  
Timothy W. JONES ◽  
Elizabeth A. DAVIS ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Vascular Function ◽  
Healthy Subjects ◽  
Glycated Haemoglobin ◽  
Tolerance Test ◽  
Oral Glucose ◽  
Insulin Levels ◽  
Glucose Levels ◽  
The Impact

The aim of the present study was to investigate the impact of an oral glucose load on circulating insulin and glucose levels and arterial function in healthy non-diabetic subjects. Thirty-nine non-obese, healthy subjects (24 female, 15 male), aged 21.0±1.8 years of age, were randomly assigned to undergo either an OGTT (oral glucose tolerance test; 75 g of glucose) or administration of a placebo. Analyses of lipids, liver function and HbA1c (glycated haemoglobin) at baseline revealed results which were within the standard reference range. Insulin and glucose levels as well as vascular function [FMD (flow-mediated dilation)] were measured at 0, 60 and 120 min. Compared with baseline, the control subjects did not exhibit any significant changes in glucose or insulin levels, whereas, in the OGTT group, blood glucose levels at both 60 (5.4±1.7 mmol/l) and 120 (5.0±1.1 mmol/l) min increased significantly relative to baseline (4.1±0.4 mmol/l; both P<0.001) and, similarly, insulin levels were higher at both 60 (30.1±21.3 m-units/l) and 120 (34.9±23.6 m-units/l) min compared with baseline (4.7±4.3 m-units/l; both P<0.001). Although blood glucose and insulin levels changed, FMD did not significantly differ between time-points or between groups. In summary, despite significantly elevated glucose and insulin concentrations in these subjects, we observed no change in vascular function, suggesting that acute elevations of glucose and insulin within the clinically normal range are not associated with impaired vascular function in vivo.

The Anti-Diabetic and Anti-Hyperlipidemia effect of Citrus maxima Fruit Peel extract in Streptozotocin - Induced Diabetic Rats

2021 ◽  
pp. 5355-5358
Author(s):  
Shaimaa M. Mohammed ◽  
Afnan E. Abd-Almonuim ◽  
Ahmed Majeed ◽  
Haithm Khlaf
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Fruit Peel ◽  
Blood Samples ◽  
Citrus Maxima

The ethanol extract of Citrus maxima fruit peel was evaluated for its hypoglycaemic and hypolipidemic activity in normal and Streptozotocin (STZ)-induced diabetic rats, using fasting and glucose tolerance test measurements. Experiments were performed using Thirty-Two Male Wister albino rats randomly divided into 4 groups and each group have 8 animals. Group1 assigned as a control injected with normal saline only. Group 2 assigned as a diabetic control injected with Streptozotocin 50mg/Kg, Group 3 assigned for diabetic + Citrus maxima in a dose of 400mg/Kg, Group 4 is assigned for the diabetic + Citrus maxima in a dose of 600mg/Kg. The Streptozotocin is injected intraperitonially to all animal in the groups except the control group. Blood samples were collected from animal before and at 21th day end of the study period. Body weight, blood glucose, serum total cholesterol (TC), triglyceride (TG), and HDL cholesterol were analysed using diagnostic kits. Serum was separated from blood samples collected. In addition oral glucose tolerance test was performed in overnight fasted control animals. Results showed that Citrus maxima extract possesses significant antidiabetic activity against streptozotocin induced diabetic rats by decreasing blood glucose levels, maintaining body weight, and serum lipid concentrations to approximate normal level. Furthermore, the extract of the title plant possesses dose dependent antidiabetic activity.

Relation between plasma insulin and blood glucose in a cross-sectional population study of the oral glucose tolerance test

1983 ◽  
Vol 102 (4) ◽  
pp. 549-556 ◽  
Author(s):  
K. Berntorp ◽  
E. Trell ◽  
J. Thorell ◽  
B. Hood
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
Plasma Insulin ◽  
Tolerance Test ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Cross Sectional ◽  
Glucose Levels ◽  
Glucose Tolerance Tests

Abstract. In a material of 3596 oral glucose tolerance tests (OGTT) performed in a population investigation of middle-aged males in Malmö, fasting and 120 min values of blood glucose and plasma insulin immunoreactivity (IRI) were studied while taking factors like body weight, smoking, alcohol, gastric resection and selfreported diabetes heredity into account. The fasting as well as the 120 min levels of both glucose and IRI were markedly influenced by body weight and smoking habits but not by the hereditary background. At 120 min, but not in the fasting state, there was a linear correlation between the IRI and glucose levels. The increase of IRI on glucose was significantly steeper in most of the hereditary subjects in comparison with their non-hereditary controls.

scholarly journals Assessment of In-vivo Anti-diabetic and Anti-diarrheal Effects of Flemingia stricta Roxb. Leaf.

2020 ◽  
Author(s):  
Shahrear Biozid ◽  
Mohammad Nazmul Alam ◽  
Jainul Abeden ◽  
Faruk ◽  
Ahmad Ibtehaz Chowdhury ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Castor Oil ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Diabetic Mice ◽  
Duration Of Treatment ◽  
Standard Drug

Objective: To evaluate the anti-diabetic and anti-diarrheal activity of methanol extract of Flemingia stricta Roxb. (Fabaceae) leaf.Methods: In anti-diabetic study, the extract was administered to alloxan-induce diabetic mice at two concentrations (200mg/kg and 400mg/kg body weight) for acute (12 hours) and prolong treatments (15 days) and blood glucose levels (Blood glucose level) of diabetic mice were monitored at intervals of hours and days throughout the duration of treatment. Antidiarrheal test was conducted by castor oil induced diarrhea and enteropooling as well as intestinal motility in mice at three different concentration (100mg/kg, 200mg/kg and 400mg/kg body weight).Results: Treatment of alloxan induce diabetic mice with the extract caused a significant reduction in fasting blood glucose level of the diabetic mice both in acute (12 hours) and prolong treatment (15 days) and it was determined that the extract at both concentration (200mg/kg and 400mg/kg) showed the significant (P&lt;0.05) hypoglycemic effect in comparison to the standard drug Metformin (10mg/kg). In the case of castor oil induced diarrheal test, enteropooling test and gastrointestinal motility test, the extract of F. stricta at 100mg/kg, 200mg/kg and 400mg/kg has given significant effect (P&lt;0.05) in comparing to standard drug Loperamide (5mg/kg). Conclusion: These result suggested that the methanol extract of F. stricta Roxb. possess promising anti-diabetic effect on alloxan-induced mice and significant antidiarrheal effect on castor oil induced diarrheal mice.

scholarly journals Antidiabetic activity of Centella asiatica (L.) urbana in alloxan induced Type 1 diabetic model rats

2012 ◽  
Vol 19 ◽  
pp. 23-27 ◽  
Author(s):  
Sonia Rahman ◽  
MAH Mostofa Jamal ◽  
Anzana Parvin ◽  
Md Mahfuz-Al-Mamun ◽  
M Rezuanul Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Water Intake ◽  
Centella Asiatica ◽  
Diabetic Control ◽  
Antidiabetic Activity ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Standard Drug ◽  
Serum Glutamate

Context: Diabetes mellitus is becoming a major burden upon healthcare facilities in all affected countries. Current therapies used for diabetics have side effects, so the current shift to the use of herbal preparations may be more effective, relatively low cost, less side effect and low toxicity. Objective: The present research was made to investigate the potential antidiabetic activity of Centella asiatica (L.) Urb. in alloxen induced diabetics. Materials and Methods: Rats were divided into 6 groups and C. asiatica was administered containing 50, 100 and 200 mg/kgbwt powder, respectively in 1ml water orally in group A, B and C rats. Metformin (150 mg/kgbwt) used as a reference standard drug. Blood glucose (BG), triglycerides (TG), total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were estimated from the serum by using standard kits. Results: C. asiatica juice had shown significant lowered the blood glucose levels in all groups. In addition, body weight, organ (liver, kidney, heart and pancreas) weight, food intake, water intake were also examined in all treated groups and compared against diabetic control group. After 22 days daily administration of C. asiatica, diabetic treated rats showed improvement in body weight, water intake as compared to diabetic control rats. In alloxan induced diabetic rats the maximum reduction in BG, TG, TC, HDL, LDL, SGOT and SGPT were observed at a dose level of 50 mg/kgbwt. Conclusion: The present data indicates that C. asiatica juice possesses potential as an antidiabetic action. DOI: http://dx.doi.org/10.3329/jbs.v19i0.12996 J. bio-sci. 19: 23-27, 2011

scholarly journals The Effects of Sleeve Gastrectomy on Glucose Metabolism and Glucagon-Like Peptide 1 in Goto-Kakizaki Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Laiyuan Li ◽  
Xiaolin Wang ◽  
Liangliang Bai ◽  
Huichuan Yu ◽  
Zenghong Huang ◽  
...  
Keyword(s):  
Body Weight ◽  
Sleeve Gastrectomy ◽  
Blood Glucose ◽  
Food Intake ◽  
Glucose Metabolism ◽  
Tolerance Test ◽  
Oral Glucose ◽  
Gk Rats ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Purpose. To investigate the effects of sleeve gastrectomy (SG) on glucose metabolism and changes in glucagon-like peptide 1 (GLP-1) in Goto-Kakizaki (GK) rats. Methods. GK rats were randomly assigned to one of three groups: SG, SG pair-fed plus sham surgery (PF-sham), and ad libitum-fed no surgery (control). Food intake, body weight, blood glucose, GLP-1 and insulin levels, and GLP-1 expression in the jejunum and ileum were compared. Results. The SG rats exhibited lower postoperative food intake, body weight, and fasting glucose than did the control rats (P<0.05). SG significantly improved glucose and insulin tolerance (P<0.05). Plasma GLP-1 levels were higher in SG rats than in control or PF-sham rats in the oral glucose tolerance test (OGTT) (P<0.05). Blood glucose levels expressed as a percentage of baseline were higher in SG rats than in control rats after exendin (9-39) administration (P<0.05). The levels of GLP-1 expression in the jejunum and ileum were higher in SG rats than in PF-sham and control rats (P<0.05). Conclusions. Improvement of glucose metabolism by SG was associated with increased GLP-1 secretion. SG contributes to an increase in plasma GLP-1 levels via increased GLP-1 expression in the mucosa of the jejunum and/or ileum.

scholarly journals Evaluation of Antidiabetic Activity of the Leaf Latex of Aloe pulcherrima Gilbert and Sebsebe (Aloaceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Gedefaw Getnet Amare ◽  
Birhanu Geta Meharie ◽  
Yaschilal Muche Belayneh
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Inhibitory Activity ◽  
Lipid Level ◽  
Radical Scavenging ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Diabetic Mice ◽  
Glucose Reduction

The leaf latex of Aloe pulcherrima has been used as remedy for diabetes mellitus. This was carried out to determine in vitro and in vivo antidiabetic activities of the leaf latex of Aloe pulcherrima. Methods. Sucrase and maltase inhibitory activity of the leaf latex of A. pulcherrima was determined in glucose oxidase assay, and α-amylase inhibitory activity was determined in dinitrosalicylic acid assay. Normoglycemic, glucose-loaded, and streptozotocin-induced diabetic mice were treated orally to determine blood glucose lowering activity of the latex. Effect of the latex on serum lipid level and body weight was measured in streptozotocin-induced diabetic mice. Additionally, DPPH assay was used to determine free radical scavenging capacity of the latex. Results. Antioxidant activity of the latex was concentration dependent; the strongest inhibition was measured at 800 μg/ml (80.57%). The leaf latex of A. pulcherrima inhibited sucrase (IC50 = 2.92 μg/ml), maltase (IC50 = 11.81 μg/ml) and α-amylase (IC50 = 14.92 μg/ml) enzymes. All doses of the leaf latex induced hypoglycemic effect after 4 h in normal mice, and low dose of the latex did not show significant effect after 6 h. Glucose reduction of the leaf latex of A. pulcherrima was significant (p<0.05) in oral glucose-loaded mice compared to the vehicle control. Blood glucose level of diabetic mice was significantly (p<0.05) reduced on week one and weak two in a streptozotocin-induced diabetic mouse model. Glucose reduction increased with increasing the doses of the leaf latex of A. pulcherrima on week one (p<0.05 (200 mg/kg), p<0.01 (400 mg/kg), and p<0.001 (600 mg/kg)). Administration of the leaf latex of A. pulcherrima for two weeks significantly (p<0.05) improved diabetic dyslipidemia and body weight of diabetic mice. Conclusion. The study confirmed that the leaf latex of the plant showed a significant antidiabetic activity justifying the traditional uses of the plant.

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