The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model

2020 ◽  
Vol 15 (6) ◽  
pp. 401-412
Author(s):  
Murat Karamese ◽  
Hakan Aydin ◽  
Volkan Gelen ◽  
Emin Sengul ◽  
Selina Aksak Karamese
Keyword(s):  
Albino Rats ◽  
Protective Effects ◽  
Treatment Groups ◽  
Probiotic Treatment ◽  
Serum Aspartate Aminotransferase ◽  
Anti Oxidant ◽  
Staining Methods ◽  
Anti Inflammatory ◽  
Wistar Albino Rats ◽  
The Relationship

Aim: The objective of this study was to evaluate the possible protective effects of probiotic bacteria, especially Bifidobacterium and Lactobacillus strains, on 4,4′-dichlorodiphenyltrichloroethane (DDT)-induced toxicity. For this reason, we evaluated the relationship between probiotics and toxicity by checking immunological and immunohistochemical parameters. Materials & methods: Probiotic pretreatment was applied to 36 Wistar albino rats for 12 consecutive days. Serum aspartate aminotransferase and alanine aminotransferase levels were detected. CD3 and NF-κB staining methods were then performed by immunohistochemistry. Finally, pro- and anti-inflammatory cytokines were measured by ELISA. Results: DDT caused a serious increase/decrease in some cytokine parameters. The effective dose was 1 × 1011 colony-forming unit probiotic treatment. CD3 and NF-κB positivity were intense in DDT group whereas the intensity was reduced in probiotic treatment groups. Discussion: The probiotic mixture has a potential to prevent inflammatory and oxidative stress related organ injuries. Further studies should be performed to explain the possible mechanisms.

scholarly journals Impact of Pycnogenol on Acetaminophen-Induced Hepatorenal Damage

2021 ◽  
Vol 12 (6) ◽  
pp. 8070-8080
Keyword(s):  
Inflammatory Cytokines ◽  
Kidney Tissue ◽  
Albino Rats ◽  
Myeloperoxidase Activity ◽  
Protective Effects ◽  
Tissue Samples ◽  
Liver And Kidney ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

We investigated the protective effects of pycnogenol (PYC), a natural anti-oxidant with an anti-inflammatory effect, on the acetaminophen (APAP)-induced hepatorenal injury in rats. Wistar albino rats were divided into four experimental groups: control, PYC (10 mg/kg, ip), APAP (1000 mg/kg), and APAP+PYC groups. Rats were decapitated 24 hours after the APAP injection, and their blood was taken to determine blood urea nitrogen (BUN), creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and pro-inflammatory cytokines; TNF-α and IL-1 β. Liver and kidney tissue samples were obtained for the histological examination and the determination of malondialdehyde (MDA) and glutathione (GSH) levels as well as myeloperoxidase (MPO) and Na+/K+-ATPase activities. PYC treatment decreased the APAP-induced elevations in serum pro-inflammatory cytokines and reduced the impairment of liver and kidney functions. Furthermore, the increase in tissue lipid peroxidation and myeloperoxidase activity and the decrease in the GSH levels and Na+/K+-ATPase activity by the APAP overdose were reversed by the PYC treatment. Besides, histologic findings reinforce the protective effect of PYC in APAP-induced hepatorenal damage. PYC, which appears to have restored the GSH and depressed neutrophil infiltration and the associated release of pro-inflammatory cytokines, merits consideration as an anti-oxidant and anti-inflammatory agent in preventing APAP-induced hepatorenal damage.

scholarly journals Ghrelin against alendronate-induced gastric damage in rats

2005 ◽  
Vol 187 (3) ◽  
pp. 399-406 ◽  
Author(s):  
S Ö Íşeri ◽  
G Şener ◽  
M Yüksel ◽  
G Contuk ◽  
Ş Çetinel ◽  
...  
Keyword(s):  
Protective Action ◽  
Collagen Content ◽  
Gastric Damage ◽  
Albino Rats ◽  
Amino Acid Peptide ◽  
Tnf Α ◽  
Anti Oxidant ◽  
Primary Amino ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

Alendronate sodium, a primary amino bisphosphonate, is widely used in the treatment of various diseases that are associated with bone resorption, such as postmenopausal osteoporosis and Paget’s disease of bone. Although the adverse effects of biphosphonates on the gastrointestinal system have been demonstrated in experimental and clinical studies, the exact mechanisms underlying this damage are not clear yet. Ghrelin, a 28 amino acid peptide produced predominantly by the stomach, was shown to exert a potent protective action on the stomach of rats exposed to ethanol or stress. Our objective was to evaluate the possible anti-oxidant and anti-inflammatory effects of ghrelin against alendronate-induced gastric damage. Wistar albino rats were administered alendronate (20 mg/kg) by gavage for 4 days, along with either ghrelin (10 ng/kg per day) or saline given i.p. After decapitation, stomach tissues were removed for the determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and tissue collagen content, while the extent of tissue damage was analyzed microscopically. Formation of reactive oxygen species was determined by chemiluminesence using a luminol probe in fresh gastric tissues. Serum tumor necrosis factor (TNF-α) and lactate dehydrogenase levels were assessed in trunk blood. Oral administration of alendronate-induced significant gastric damage, accompanied by increased MPO activity, collagen content, MDA and luminol levels (P< 0.01–P< 0.001), while tissue GSH was decreased (P< 0.01). On the other hand, ghrelin treatment reversed these alterations (P< 0.05–P<0.001) as well as elevating serum TNF-α levels significantly (P< 0.001). The findings of the present study suggest that alendronate induces oxidative gastric damage by a local irritant effect, and ghrelin ameliorates this damage by its possible antioxidant and anti-inflammatory properties.

scholarly journals Protective Effect of Ethanolic Extract ofTabernaemontana divaricata(L.) R. Br. against DEN and Fe NTA Induced Liver Necrosis in Wistar Albino Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kannappan Poornima ◽  
Palanisamy Chella Perumal ◽  
Velliyur Kanniappan Gopalakrishnan
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Protective Effects ◽  
Liver Necrosis ◽  
Standard Drug ◽  
Tabernaemontana Divaricata ◽  
Wistar Albino Rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

Phenolic profile, Anti-inflammatory and Diuretic properties of Asplenium ceterach tested on Albino mice and Wistar albino rats

2020 ◽  
Vol 13 (6) ◽  
pp. 449-454
Author(s):  
Rekia Sidhoum ◽  
Mostapha Bachir bey ◽  
Latifa Halli ◽  
Othmane Yalaoui ◽  
Asma Belkadi
Keyword(s):  
Albino Rats ◽  
Phenolic Profile ◽  
Albino Mice ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

scholarly journals Analgesic and Anti-Inflammatory Activity of Total Crude Leaf Extract of <i>Phytolacca dodecandra</i> in Wistar Albino Rats

2019 ◽  
Vol 10 (03) ◽  
pp. 259-271
Author(s):  
Loyce Nakalembe ◽  
Josephine N. Kasolo ◽  
Edward Nyatia ◽  
Aloysius Lubega ◽  
Godfrey S. Bbosa
Keyword(s):  
Leaf Extract ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Phytolacca Dodecandra ◽  
Crude Leaf Extract ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

scholarly journals Protective effects of rosmarinic acid on sepsis-induced DNA damage in the liver of Wistar albino rats

2015 ◽  
Vol 6 ◽  
Author(s):  
Goktas Hatice ◽  
Bacanli Merve ◽  
Aydin Sevtap ◽  
Taner Gokce ◽  
Sahin Tolga ◽  
...  
Keyword(s):  
Dna Damage ◽  
Rosmarinic Acid ◽  
Albino Rats ◽  
Protective Effects ◽  
Wistar Albino Rats

scholarly journals Antibacterial, Antioxidant and Anti-Inflammatory Potential of the Different Extracts of Holoptelia Integrifolia

2019 ◽  
Vol 9 (2) ◽  
pp. 5234-5240
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Albino Rats ◽  
Nitric Oxide Radical ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

Medicinal plants are play significant impact in the personal medicine for most of the people all over the world as an alternative live saving medicines and most of their medicinal properties are well known for anticancer activity. The different extracts of Holoptelea integrifolia (H.integrifolia) leaves, stem bark and fruits were studied as a potent natural source of antimicrobial, antioxidant and wound healing potential. This work was carried out to evaluate antimicrobial, antioxidant and anti-inflammatory activity of different extracts of H.integrifolia. The antimicrobial activity of the H.integrifolia ethanolic extract was studied against five fungal and bacterial strains by utilizing the agar well diffusion method and MIC. Among several strain, the ethanolic extract of fruit has shown higher antimicrobial inhibition zone as 9.25-16 mm compare to other two extracts of stem and leaves as 10- 13.25 mm and 6-10.2 mm respectively. The antioxidant activities for different extract were also determined by DPPH free radical assay, Hydroxyl Radical Scavenging and Nitric Oxide Radical Scavenging Activity method. The anti-inflammatory activity also estimated basedon formalin induced paw edema method on Wistar albino rats. The different extracts of leaves, stem bark and fruit parts of Holoptelea integrifolia were estimated for in vivo anti-inflammatory activity against the animal model of female Wistar albino rats. The results of anti-inflammatory activity revealed that the Ethanol extracts showed vital and dose-dependent anti-inflammatory effects. Our findings revealed that aerial parts of H.integrifolia contais potential antimicrobial, antioxidant and anti-inflammatory compounds, which expose the medicinal potential of the selected plant could be a significant drug candidates against microbial, oxidative and inflammation-related pathological processes as a future alternative medicine.

Anti-Apoptotic and Anti-Inflammatory Activities of Edible Fresh Water Algae Prasiola japonica in UVB-Irradiated Skin Keratinocytes

2019 ◽  
Vol 47 (08) ◽  
pp. 1853-1868
Author(s):  
Eunju Choi ◽  
Young-Su Yi ◽  
Jongsung Lee ◽  
Sang Hee Park ◽  
Sunggyu Kim ◽  
...  
Keyword(s):  
Fresh Water ◽  
Ethanol Extract ◽  
The Body ◽  
Hacat Cells ◽  
Protective Effects ◽  
Proteolytic Activation ◽  
Skin Keratinocytes ◽  
External Stresses ◽  
Anti Oxidant ◽  
Anti Inflammatory

Skin is the outer tissue layer and is a barrier protecting the body from various external stresses. The fresh water green edible algae Prasiola japonica has antiviral, antimicrobial, and anti-inflammatory properties; however, few studies of its effects on skin-protection have been reported. In this study, Prasiola japonica ethanol extract (Pj-EE) was prepared, and its skin-protective properties were investigated in skin keratinocytes. Pj-EE inhibited ROS production in UVB-irradiated HaCaT cells without cytotoxicity. Pj-EE also suppressed the apoptotic death of UVB-irradiated HaCaT cells by decreasing the generation of apoptotic bodies and the proteolytic activation of apoptosis caspase-3, -8, and -9. Moreover, Pj-EE downregulated the mRNA expression of the inflammatory gene cyclooxygenase-2 (COX-2), the pro-inflammatory cytokine genes interleukin (IL)-1[Formula: see text], IL-8, IL-6, tumor necrosis factor (TNF)-[Formula: see text], and interferon (IFN)-[Formula: see text], and the tissue remodeling genes matrix metalloproteinase (MMP)-1, -2, -3, and -9. The Pj-EE-induced anti-inflammatory effect was mediated by suppressing the activation of nuclear factor-kappa B (NF-[Formula: see text]B) signaling pathway in the UVB-irradiated HaCaT cells. Taken together, these results suggest that Pj-EE exerts skin-protective effects through anti-oxidant, anti-apoptotic, and anti-inflammatory activities in skin keratinocytes.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF AERIAL PART EXTRACTS OF PLANTAGO MAJOR L.

2017 ◽  
Vol 10 (9) ◽  
pp. 307
Author(s):  
Jyoti Vandana ◽  
Gupta A K ◽  
Alok Mukerjee
Keyword(s):  
Aerial Part ◽  
Iridoid Glycosides ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Plantago Major ◽  
Phytochemical Screening ◽  
Paw Edema ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

  Objective: This study aims to perform phytochemical screening and investigate anti-inflammatory activity of extract from aerial part of the plant Plantago major L.Methods: Preliminary phytochemical screening of plant extract was carried out using standard qualitative procedures and anti-inflammatory activity was evaluated using carrageenan-induced paw edema model in male Wistar albino rats using plethysmometer. Diclofenac sodium (10 mg/kg, b.w.) was used as standard drug.Results: Preliminary phytochemical screening of the different extracts revealed that they were rich in secondary metabolite compounds such as carbohydrates, tannins, steroids, flavonoids, terpenoids, glycosides, fats and oils, alkaloids, phenolic compounds, and iridoid glycosides. The anti-inflammatory activity was evaluated using carrageenan-induced paw edema models in Wistar albino rats. The anti-inflammatory activity of methanolic extract (ME) was found to be dose dependent in carrageenan-induced paw edema model. The ME has shown significant (p<0.05) inhibition of paw edema, 61.11% and 55.55% on 4th hr at the doses of 900 and 600 mg/kg, respectively.Conclusion: The results of the present study demonstrate that ME of the aerial parts of the plant possess significant (p<0.05) anti-inflammatory potential.

Sign in / Sign up

Export Citation Format

Share Document