scholarly journals A Salute to PVT TIM HiLL: Indispensable amino acids and global human health

2021 ◽  
Author(s):  
Eric Berg ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Protein Quality ◽  
Ground Beef ◽  
Animal Origin ◽  
Life Stages ◽  
Food Labels ◽  
Food Ingredients ◽  
Quality Item ◽  
Ground Pork ◽  
Indispensable Amino Acids

Students are taught the study mnemonic PVT TIM HiLL to memorize nineindispensable amino acids (IAA); Phenylalanine, Valine, Threonine, Tryptophan,Isoleucine, Methionine, Histidine, Leucine, and Lysine.  The Food and Animal Organization (FAO)recommended IAA be treated as individual nutrients on food labels because‘crude protein’ alone does not indicate protein quality.  Protein quality, determined by digestible IAAscore (DIAAS) is calculated for three life stages; birth to 6 months; 6 monthsto 3 years, and 3 years and older.  Foodspossessing DIAAS greater than 100 are “excellent” protein quality, DIAASbetween 75 and 99 are ‘good’, while foods with DIAAS < 75 cannot make aprotein claim.  Processing, heating,and(or) grinding can decrease or improve plant and animal IAA digestibility.For children six months to three years, ground pork, smoked-cooked bacon,cooked pork leg, cured ham, cooked pork loin, salami, beef/pork bologna, beefjerky, and medium and medium rare beef ribeye steaks can be described‘excellent’.  Cooked ground beef can beclassified as ‘good’ or ‘excellent’ depending on the study, while theImpossible and Beyond meat-alternative burgers and well-done ribeye can beclassified as ‘good’ sources of IAA for young children.  For persons > 3 years, all meat categoriesbut cooked ground beef can be classified ‘excellent’ sources of IAA.  For meat alternatives, Impossible Burger®could be classified as ‘excellent’ but Beyond Burger® could only claim to be‘good’.  Protein quality claims forindividual food ingredients can be diminished when consumed with a lowerprotein quality item such as a wheat flour bun. To provide meals that are adequate in all IAA, the protein quality ineach food item must be determined.  Mixedmeals must be adjusted for protein quality by combining low quality proteins(present in cereals and grains) with higher quality proteins (present in foodsof animal origin).

scholarly journals Available lysine and digestible amino acid contents of proteinaceous foods of India

2012 ◽  
Vol 108 (S2) ◽  
pp. S59-S68 ◽  
Author(s):  
Shane M. Rutherfurd ◽  
Kiran Bains ◽  
Paul J. Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Quality ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Food Ingredients ◽  
Available Lysine ◽  
Amino Acid Contents ◽  
Sulphur Amino Acids ◽  
Prepared Food

Cereals and legumes are staple foods in India and are limiting in lysine and sulphur amino acids, respectively. Available lysine loss, due to Maillard-type reactions that may occur during food preparation, exacerbates the problem of lysine deficiency particularly in cereals. Consequently, determining the contents of digestible essential amino acids, particularly lysine, is important. True ileal digestibilities of most amino acids (including total and reactive lysine) were determined for ten food ingredients and eleven foods commonly consumed in India. Semi-synthetic diets each containing either an ingredient or the prepared food as the sole protein source were formulated to contain 100 g kg− 1protein (75 g kg− 1for rice-based diets) and fed to growing rats. Titanium dioxide was included as an indigestible marker. Digesta were collected and the amino acid content (including reactive lysine) of diets and ileal digesta determined. Available (digestible reactive) lysine content ranged from 1·9–15·4 g kg− 1and 1·8–12·7 g kg− 1across the ingredients and prepared foods respectively. True ileal amino acid digestibility varied widely both across ingredients and prepared foods for each amino acid (on average 60–92 %) and across amino acids within each ingredient and prepared food (overall digestibility 31–96 %). Amino acid digestibility was low for many of the ingredients and prepared foods and consequently digestibility must be considered when assessing the protein quality of poorer quality foods. Given commonly encountered daily energy intakes for members of the Indian population, it is estimated that lysine is limiting for adults in many Indian diets.

Nutritive and Bioactive Proteins in Breastmilk

2020 ◽  
Vol 18 (5) ◽  
pp. 13-22
Author(s):  
Ferdinand Haschke ◽  
◽  
Nadja Haiden ◽  
Sagar K. Thakkar ◽  
◽  
...  
Keyword(s):  
Amino Acids ◽  
Breast Milk ◽  
Protein Quality ◽  
The Body ◽  
Gut Development ◽  
Low Birth Weight Infants ◽  
Term Infants ◽  
Protein Supplements ◽  
Bioactive Proteins ◽  
Indispensable Amino Acids

Protein ingested with breast milk provides indispensable amino acids which are necessary for new protein synthesis for growth and replacement of losses via urine, feces, and the skin. Protein gain in the body of an infant is highest during the first months when protein concentrations in breast milk are higher than during later stages of lactation. Low-birth-weight infants have higher protein needs than term infants and need protein supplements during feeding with breastmilk. Based on our better understanding of protein evolution in breastmilk during the stages of lactation, new infant formulas with lower protein concentration but better protein quality have been created, successfully tested, and are now available in many countries. Besides providing indispensable amino acids, bioactive protein in breast milk can be broadly classified into 4 major functions, that is, providing protection from microbial insults and immune protection, aiding in digestive functions, gut development, and being carriers for other nutrients. Individual proteins and their proposed bioactivities are summarized in this paper in brief. Indeed, some proteins like lactoferrin and sIgA have been extensively studied for their biological functions, whereas others may require more data in support to further validate their proposed functions. Keywords: protein requirements for growth, nutritive proteins, bioactive proteins, bioactive peptides, infants, breastmilk, lactoferrin, immunoglobulins

scholarly journals Use of the precision-fed cecectomized rooster assay to determine standardized amino acid digestibility, true metabolizable energy content, and digestible indispensable amino acid scores of plant-based protein by-products used in canine and feline diets

2021 ◽  
Author(s):  
Lauren M Reilly ◽  
Patrick C von Schaumburg ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Pamela L Utterback ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Quality ◽  
Energy Content ◽  
Metabolizable Energy ◽  
Pet Food ◽  
Indispensable Amino Acid ◽  
Indispensable Amino Acids ◽  
Limiting Amino Acid ◽  
By Products

Abstract Traditionally, protein by-products from oil seeds and cereal grains have been used in pet foods as sustainable, inexpensive, and protein-rich ingredients. However, the on-going demonization of soy- and corn-based ingredients continue to hinder their use in pet food and treat formulations. Ideally, the further demonstration of their protein quality and nutrient composition may encourage their favorable return as acceptable ingredients in pet foods and treats. Therefore, the objectives of this study were to determine the macronutrient composition, indispensable amino acid profile, standardized amino acid digestibility, true metabolizable energy content corrected for nitrogen (TMEn), and digestible indispensable amino acid scores (DIAAS-like) of soy flakes (SF), peanut flour (PF), soybean meal (SBM), and corn gluten meal (CGM). Standardized amino acid digestibility was assessed using the precision-fed cecectomized rooster assay. All test ingredients demonstrated a profile of highly digestible indispensable amino acids except for lysine in PF, which was lowest (P < 0.05) at 45.5%. The SBM and CGM had the highest (P > 0.05) digestibilities of indispensable amino acids. A DIAAS-like values was calculated for each ingredient using either AAFCO (2020) recommended values or NRC (2006) recommended allowances as the reference protein pattern. For adult dogs compared to AAFCO recommended values, the first-limiting amino acid was lysine for PF and CGM but it was methionine for SF and SBM. For adult cats compared to AAFCO recommended values, the first-limiting amino acid was lysine for PF and CGM but it was threonine for SF. There was no first-limiting amino acid in SBM for cats as DIAAS-like values were over 100% for all indispensable amino acids. The TMEn values were highest (P < 0.05) for PF and CGM (4.58 and 4.31 kcal/g [dry matter basis], respectively). The protein quality of these plant-based protein by-products reflect their value as nutritional ingredients for canine and feline diets. However, the prior processing of these by-products must be considered before exposing them to additional processing methods, such as extrusion. Additionally, the inclusion of complementary proteins or supplemental amino acids will be needed to meet all indispensable amino acid requirements for a nutritionally complete and balanced pet food.

scholarly journals Nutritive and Bioactive Proteins in Breastmilk

2016 ◽  
Vol 69 (Suppl. 2) ◽  
pp. 16-26 ◽  
Author(s):  
Ferdinand Haschke ◽  
Nadja Haiden ◽  
Sagar K. Thakkar
Keyword(s):  
Amino Acids ◽  
Breast Milk ◽  
Protein Quality ◽  
The Body ◽  
Gut Development ◽  
Low Birth Weight Infants ◽  
Term Infants ◽  
Protein Supplements ◽  
Lower Protein ◽  
Indispensable Amino Acids

Protein ingested with breast milk provides indispensable amino acids which are necessary for new protein synthesis for growth and replacement of losses via urine, feces, and the skin. Protein gain in the body of an infant is highest during the first months when protein concentrations in breast milk are higher than during later stages of lactation. Low-birth-weight infants have higher protein needs than term infants and need protein supplements during feeding with breastmilk. Based on our better understanding of protein evolution in breastmilk during the stages of lactation, new infant formulas with lower protein concentration but better protein quality have been created, successfully tested, and are now available in many countries. Besides providing indispensable amino acids, bioactive protein in breast milk can be broadly classified into 4 major functions, that is, providing protection from microbial insults and immune protection, aiding in digestive functions, gut development, and being carriers for other nutrients. Individual proteins and their proposed bioactivities are summarized in this paper in brief. Indeed, some proteins like lactoferrin and sIgA have been extensively studied for their biological functions, whereas others may require more data in support to further validate their proposed functions.

scholarly journals The Complementarity of Amino Acids in Cooked Pulse/Cereal Blends and Effects on DIAAS

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1999
Author(s):  
Fei Han ◽  
Paul James Moughan ◽  
Juntao Li ◽  
Natascha Stroebinger ◽  
Shaojie Pang
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mung Bean ◽  
Protein Quality ◽  
Latin Square ◽  
Growing Pigs ◽  
Adzuki Bean ◽  
Ileal Digestibility ◽  
Indispensable Amino Acids ◽  
The Mean

The aim was to study the complementary effect between cereals and pulses on protein quality. The values for the digestible indispensable amino acid score (DIAAS) in cooked cereals and pulses, given alone, and blends of cooked cereals and pulses, were determined. True ileal digestibility (TID) values of amino acids for adult humans were obtained. It is difficult to determine ileal amino acid digestibility in humans directly, and for this reason, the growing pig is often used to obtain such values, as a preferred animal model. Seven growing pigs fitted with a T-cannula at the terminal ileum were allotted to a 7 × 6 incomplete Latin square with seven semi-synthetic diets (cooked mung bean, adzuki bean, millet, adlay, mung bean + millet, adzuki bean + adlay, and an N-free diet) and six 7-day periods. The mean TID values for crude protein differed significantly (p < 0.05), with millet having the highest digestibility (89.4%) and the adzuki bean/adlay mixture having the lowest (79.5%). For lysine, adzuki bean had the highest TID (90%) and millet had the lowest (70%). For the mean of all the amino acids, there was a significant (p < 0.05) effect of diet, with the TID ranging from 72.4% for the adzuki bean/adlay mixture to 89.9% for the adzuki beans. For the older child, adolescent, and adult, the DIAAS (%) was 93 for mung beans, 78 for adzuki beans, 22 for millet, 16 for adlay, and 66 for mung beans + millet, and 51 for adzuki beans + adlay. For mung beans, valine was first-limiting, and the SAA for adzuki beans, while lysine was first-limiting for the other foods. Chinese traditional diets, containing both cereals and pulses, are complementary for most, but not all of the indispensable amino acids.

scholarly journals Are Dietary Amino Acids or Protein Quality Associated with Infant Length Gain from 6 to 12 Months in Rural Malawi? (P10-010-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Andrew Matchado ◽  
Kathryn Dewey ◽  
Christine Stewart ◽  
Per Ashorn ◽  
Ulla Ashorn ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Breast Milk ◽  
Protein Quality ◽  
Complementary Food ◽  
Complementary Foods ◽  
Acid Intake ◽  
Dietary Amino Acid ◽  
Amino Acid Intake ◽  
Length Gain

Abstract Objectives 1) to estimate the probability of inadequate amino acid intake among infants 9–10 months of age in rural Malawi 2) to evaluate whether dietary amino acid intake or protein quality are associated with length gain from 6 to 12 months of age Methods We assessed total amino acid intake from breast milk and complementary foods in 285 infants. Breast milk intake and complementary foods were estimated using dose-to-mother deuterium oxide dilution method and repeat 4-pass interactive 24-hour recall interviews, respectively. Amino acid composition values were taken from FAO human milk profile, Tanzania Food Composition table and International Minilist. Protein quality was estimated using Digestible Indispensable Amino Acid Score (DIAAS). Probability of intake below Estimated Average Requirement (EAR) for each amino acid was estimated using National Cancer Institute (NCI) method. We estimated protein quality of complementary food using median DIAAS. We assumed a DIAAS of ≥0.75 to represent a diet or food with good protein quality. Relationships between amino acid intake or protein quality with length gain were assessed using regression models. Length was measured at 6 and 12 months of age and length for age z-score (LAZ) velocity was calculated (ΔLAZ/months). Results The probability of inadequate amino acid intake from breast milk and complementary food that included a lipid-based nutrient supplement (LNS) was 3% for lysine, 0% for tryptophan, threonine, valine, histidine, isoleucine, leucine, sulfur containing amino acids (SAA), and aromatic amino acids (AAA). Without LNS, the probability was 7% for lysine and 0–2% for the other amino acids. The median (interquartile range) DIAAS for complementary food with and without LNS was 0.70 (0.28) and 0.64 (0.32), respectively. Dietary amino acid intake and protein quality were not significantly associated with length gain velocity from 6 to 12 months even after adjusting for confounding factors. Conclusions The prevalence of inadequate amino acid intake in 9–10 months old infants in rural Malawi is very low. However, in conditions of frequent clinical or sub-clinical infections this situation may be different. Linear growth at 6–12 months does not appear to be limited by dietary amino acid intake or protein quality in this setting. Funding Sources The Bill & Melinda Gates Foundation.

scholarly journals Effect of Time and Legume Type on Germination-Induced Proteolysis of Lentils and Faba Beans

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 4
Author(s):  
Sara Bautista-Expósito ◽  
Elena Peñas ◽  
Albert Vanderberg ◽  
Juana Frias ◽  
Cristina Martínez-Villaluenga
Keyword(s):  
Amino Acids ◽  
Protease Activity ◽  
Protein Quality ◽  
Principal Component ◽  
Fold Increase ◽  
Protein Digestibility ◽  
Essential Amino Acids ◽  
Seedling Development ◽  
Positive Effects ◽  
Faba Beans

Legumes are alternative protein sources that have been successfully used to develop diverse meatless foods. Although these plant-based products have a lower impact on the environment than equivalent animal-based products, they have lower protein digestibility. Germination could be a useful bioprocess to enhance protein digestibility in legumes, although its effect at different times of seedling development has been little studied in lentils and faba beans. This work investigated the effect of germination time (4 and 6 days after full seed imbibition) on the proteins of three types of Canadian lentils (“gray zero tannin”, G; “caviar black”, B; and “red dehulled”, D) and faba beans (“zero vicin/convicin”, F). Germination increased total nitrogen (4–14% increase) and total levels of some amino acids: Asp in all the sprouts studied; Ser, Pro, Ala, Cys, His and Lys in G; and Met and Tyr in B. A concurrent degradation of the 7S and 11S globulin subunits, the accumulation of peptides below 20 kDa and free essential and non-essential amino acids (4- to 6-fold increase) were observed after germination in all the legumes studied. These effects were attributable to the increased protease activity observed after sprouting. Trypsin inhibitory activity was lower in legume sprouts, except for D, where a small increase was detected. Time, legume type and their interaction showed significant effects on the parameters studied. Germination effects were generally more remarkable at longer stages of seedling development. Among the legumes studied, D showed a differential behavior characterized by a faster protein degradation and release of small peptides, probably due to its higher protease activity as indicated by principal component analysis. These results evidence the positive effects of germination on the protein digestibility of different lentil types and faba beans. The protein quality of plant-based foods could be improved through the selection of legume species with higher germination-induced proteolytic rates and optimized germination times.

PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Latin Square ◽  
Sole Source ◽  
Adaptation Period ◽  
Amino Acid Supplementation ◽  
Ileal Digestibility ◽  
Indispensable Amino Acids ◽  
Dietary Treatments

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P &lt; 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P &lt; 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

INFLUENCE OF DIETARY ANTIBIOTICS ON NITROGEN, AMINO ACID AND ENERGY DIGESTION BY RATS AND ADULT ROOSTERS

1982 ◽  
Vol 62 (2) ◽  
pp. 537-545 ◽  
Author(s):  
L. D. CAMPBELL ◽  
B. O. EGGUM ◽  
J. WOLSTRUP
Keyword(s):  
Amino Acids ◽  
Dietary Protein ◽  
Protein Quality ◽  
Positive Influence ◽  
Metabolizable Energy ◽  
Biological Value ◽  
Key Words Antibiotics ◽  
True Digestibility ◽  
Neomycin Sulfate ◽  
Net Protein

The influence of antibiotics (a 2:1 mixture of bacitracin and neomycin sulfate at 0.7% of the diet) on the availability of energy from barley-fishmeal-based diets was assessed using rats and adult roosters. Changes in estimates of protein quality, as influenced by dietary antibiotics were also determined in the study with rats. Gut biomass content was estimated by measuring the content of adenosine triphosphate (ATP) in gut samples of rats and fresh excreta samples of roosters. Antibiotic addition to the diet significantly (P < 0.01) reduced apparent digestible energy in rats and tended to reduce the true metabolizable energy measured with roosters. Biological value of the protein for rats was increased significantly (P < 0.01) by antibiotics as were net protein utilization values even though true digestibility of protein was decreased significantly (P < 0.01). The availability of some amino acids (e.g. histidine) showed a marked depression (12.1 percentage units) when antibiotics were added to the diet while others were not influenced (e.g. lysine and methionine) or markedly (7.6 percentage units) increased (e.g. alanine). The extent of microbial activity, which was shown to be markedly higher in the hindgut of rats fed no antibiotics as compared to rats fed antibiotics, may have contributed to the positive influence of antibiotic feeding on biological value of dietary protein and to the changes noted in excretion of amino acids by rats and roosters. Key words: Antibiotics, Nitrogen, amino acids, energy, rats, rosters

scholarly journals Evaluation of the Most Current and Effective Methods in the Analysis of Chlorinated Dioxins in Ground Beef

2003 ◽  
Vol 3 ◽  
pp. 913-921 ◽  
Author(s):  
Ebere C. Anyanwu ◽  
Mohamed H. El-Saeid ◽  
Akpan I. Akpan ◽  
Mahmoud A. Saled
Keyword(s):  
Food Chain ◽  
Environmental Pollutants ◽  
Ground Beef ◽  
Extraction Methods ◽  
Sample Introduction ◽  
Animal Origin ◽  
Clear Understanding ◽  
Daily Consumption ◽  
Contaminated Food ◽  
Chlorinated Dioxins

Chlorinated dioxins are the group of environmental pollutants consisting of 210 chlorinated dibenzo-p-dioxins and dibenzofurans. They are highly toxic and persistent. They are lipophilic and can easily biomagnify in the food chain, hence posing a serious threat to human health. The daily consumption of low-level contaminated food, mainly of animal origin, leads to the accumulation of dioxins in the human body. The exposures of the general human population to dioxins and the specific issues of a risk assessment of dioxin pose serious concerns in public environmental and nutritional health. This paper reviews the analysis of chlorinated dioxins in ground beef. The sources of contamination of chlorinated dioxins in ground beef are first reviewed to form a basis for a clear understanding of the health implications of chlorinated dioxins in the human food chain and why it is necessary to monitor the level of dioxins in animal food products, especially ground beef. The methods of collection, sampling, and processing of ground beef, and the methods of sample clean up prior to the analysis, are reviewed. Emphasis is laid on the new techniques that are available and that might be effective in the analysis of chlorinated dioxins in ground beef. Among these new methods and techniques are: the synergistic combination of ELISA/GC/MS, direct sample introduction to /GC/MS-MS, automated clean-up method, and the supercritical fluid extraction methods. The possible treatments of results from each method and technique are discussed and their respective efficiencies are compared. Finally, quality control and quality assurance parameters are evaluated for levels of accuracy, reproducibility, and precision.

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