EFFECT OF THE SYMBIOSIS BETWEEN LOLIUM MULTIFLORUM AND THE ENDOPHYTE EPICHLOË OCCULTANS ON NUTRITIONAL QUALITY OF PASTURE IN BIOSOLIDS´ AMENDED SOIL

2018 ◽  
Vol 11 (41-42) ◽  
Author(s):  
Silvana Irene Torri ◽  
María Mabel Puelles ◽  
Alexis Magali De las Nieves Ovejero

<p>Biosolids have a high content of stable organic matter, which may be used for the remediation of marginal non-agricultural soils used for pasture production. In Argentina, the aerial biomass of <em>Lolium multiflorum</em> Lam., species is highly infected with the endophyte <em>Epichloë occultans</em>. Endophytes establish a symbiotic relationship with host plants, providing protection and resistance against different stress factors. Since land application of biosolids may rise plant stress, the objective of this work was to analyze the growth and concentration of copper (Cu) and zinc (Zn) in aerial biomass of <em>L. multiflorum</em> in symbiosis (or not) with the endophyte <em>E. occultans</em> on a sandy textured soil amended with biosolids. The association of <em>L multiflorum</em> with the endophyte produced an increase in aerial biomass only in the biosolids´ amended soil. However, the presence of the endophyte did not modify the concentration of Cu or Zn in the aerial biomass of the pasture under the experimental conditions.</p>

Author(s):  
A.V. Konstantinovich ◽  
◽  
A.S. Kuracheva ◽  
E.D. Binkevich

In conditions of climate change, when temperature and precipitation fluctuations occur more and more frequently during the growing season, it is necessary to obtain high quality seedlings with "immunity" to various stress factors, including high weediness, the damage from which is associated with a decrease in yield (by 25 -35%) and with a deterioration in the quality of agricultural products. Due to the imbalance in production technology, seedlings are often weakened, overgrown, with a low yield per unit area and survival rate in the field. One of the solutions to this problem is the use of PP for pre-sowing seed treatment to increase the competitiveness of seedlings in the field.


Biochar ◽  
2021 ◽  
Author(s):  
Ngitheni Winnie-Kate Nyoka ◽  
Ozekeke Ogbeide ◽  
Patricks Voua Otomo

AbstractTerrestrial and aquatic ecosystems are increasingly threatened by pesticide pollution resulting from extensive use of pesticides, and due to the lack of regulatory measures in the developing world, there is a need for affordable means to lessen environmental effects. This study aimed to investigate the impact of biochar amendment on the toxicity of imidacloprid to life-cycle parameters and biomarker responses of the earthworm Eisenia fetida. E. fetida was exposed to 10% biochar-amended and non-amended OECD artificial soils spiked with 0, 0.75, 1.5, 2.25 and 3 mg imidacloprid/kg for 28 days. An LC50 of 2.7 mg/kg was only computed in the non-amended soil but not in the biochar-amended soil due to insignificant mortality. The EC50 calculated in the non-amended soil (0.92 mg/kg) for reproduction (fertility) was lower than the one computed in the biochar amended (0.98 mg/kg), indicating a decrease in toxicity in the biochar-amended substrate. Significant weight loss was observed at the two highest imidacloprid treatments in the non-amended soil and only at the highest treatment in the biochar-amended substrate, further highlighting the beneficial effects of biochar. Catalase activity decreased significantly at the two highest concentrations of non-amended soil. Yet, in the amended soil, the activity remained high, especially in the highest concentration, where it was significantly higher than the controls. This indicated more severe oxidative stress in the absence of biochar. In all non-amended treatments, there was a significant acetylcholinesterase inhibition, while lower inhibition percentages were observed in the biochar-amended soil. In most endpoints, the addition of biochar alleviated the toxic effects of imidacloprid, which shows that biochar has the potential to be useful in soil remediation. However, there is still a need for field studies to identify the most effective application rate of biochar for land application.


2021 ◽  
pp. 019262332198965
Author(s):  
Jeffrey C. Wolf

A number of studies have investigated the potential toxicity of the analgesic agent diclofenac (DCF) in various fish species under a diverse array of experimental conditions. Reported evidence of toxicity in these investigations is often strongly reliant on morphologic end points such as histopathology, immunohistochemistry, and transmission electron microscopy. However, it may be challenging for scientists who perform environmental hazard or risk determination to fully appreciate the intricacies of these specialized endpoints. Therefore, the purpose of the current review was to critically assess the quality of morphologic data in 14 papers that described the experimental exposure of fish to DCF. Areas of focus during this review included study design, diagnostic accuracy, magnitude of reported changes, data interpretation and presentation, and the credibility of individual reported findings. Positive attributes of some studies included robust experimental designs, accurate diagnoses, and straightforward and transparent data reporting. Issues identified in certain articles included diagnostic errors, failure to account for sampling and/or observer bias, failure to evaluate findings according to sex, exaggeration of lesion severity, interstudy inconsistencies, unexplained phenomena, and incomplete or ambiguous data presentation. It is hoped that the outcome of this review will be of value for personnel involved in regulatory decision-making.


1982 ◽  
Vol 13 (2) ◽  
pp. 121-128 ◽  
Author(s):  
Louise D. Stalnaker ◽  
Nancy A. Creaghead

Language samples were obtained from 12 Head Start preschool children under the following three experimental conditions: Condition I, Retelling a Story with Toys; Condition II, Playing with Toys; Condition III, Toys with Questions. The quantity and quality of the three samples gathered from each child were compared by analyzing the total number of utterances, the proportion of the total utterances which were sentence fragments, the number of transformations and adverbial expansions, the number of different semantic relationships, and the mean length of utterance (MLU). Results indicated that retelling a story produced the largest MLU, but toys and questions produced more utterances. These findings indicated that questioning children does not inhibit their language and asking them to retell a story may be a fruitful approach to use when gathering a language sample.


2015 ◽  
Vol 95 (4) ◽  
pp. 759-770 ◽  
Author(s):  
J. J. Miller ◽  
B. W. Beasley ◽  
C. F. Drury ◽  
F. J. Larney ◽  
X. Hao

Miller, J. J., Beasley, B. W., Drury, C. F., Larney, F. and Hao, X. 2015. Influence of long-term manure application on mineral composition of irrigated barley silage. Can. J. Plant Sci. 95: 759–770. The long-term effect of land application of manure type (composted vs. stockpiled manure), bedding type (wood-chips vs. straw), and application rate on feed quality of barley silage as feed for beef cattle is unknown. We measured selected minerals [P, Ca, Ca:P ratio, Mg, K, K:(Ca+Mg) ratio, Na, Fe, Mn, Zn, Cu] of irrigated barley silage (Hordeum vulgare L.) on a clay loam soil after 4 (2002), 7 (2005) and 11 (2009) years of annual applications of composted (CM) or stockpiled (SM) feedlot manure with wood-chips (WD) or straw (ST) bedding at three application rates (13, 39, 77Mg ha−1 dry wt.). The treatments also included an unamended control and inorganic fertilizer treatment. Manure type generally had inconsistent or no significant (P≤0.05) effect on the concentrations of these minerals in barley silage. Most crop minerals were generally greater under ST than WD. The findings for P, K, Na, and K:(Ca+Mg) ratio generally supported our hypothesis of greater crop concentrations with greater application rate, but Ca and Mg decreased at higher rates. Overall, our findings suggest that bedding and application rate have more potential than manure type for managing the feed quality of barley silage.


2006 ◽  
Vol 1 (2) ◽  
pp. 275-288 ◽  
Author(s):  
Simone Graeff ◽  
Johanna Link ◽  
Wilhelm Claupein

AbstractThe ability to identify diseases in an early infection stage and to accurately quantify the severity of infection is crucial in plant disease assessment and management. A greenhouse study was conducted to assess changes in leaf spectral reflectance of wheat plants during infection by powdery mildew and take-all disease to evaluate leaf reflectance measurements as a tool to identify and quantify disease severity and to discriminate between different diseases. Wheat plants were inoculated under controlled conditions in different intensities either with powdery mildew or take-all. Leaf reflectance was measured with a digital imager (Leica S1 Pro, Leica, Germany) under controlled light conditions in various wavelength ranges covering the visible and the near-infrared spectra (380–1300 nm). Leaf scans were evaluated by means of L*a*b*-color system. Visual estimates of disease severity were made for each of the epidemics daily from the onset of visible symptoms to maximum disease severity. Reflectance within the ranges of 490780 nm (r2 = 0.69), 510780nm (r2 = 0.74), 5161300nm (r2 = 0.62) and 5401300 nm (r2 = 0.60) exhibited the strongest relationship with infection levels of both powdery mildew and take-all disease. Among the evaluated spectra the range of 490780nm showed most sensitive response to damage caused by powdery mildew and take-all infestation. The results of this study indicated that disease detection and discrimination by means of reflectance measurements may be realized by the use of specific wavelength ranges. Further studies have to be carried out, to discriminate powdery mildew and take-all infection from other plant stress factors in order to develop suitable decision support systems for site-specific fungicide application.


2013 ◽  
Vol 671-674 ◽  
pp. 2736-2741
Author(s):  
Yin An Ming ◽  
Tao Tao

To reuse municipal sewage sludge safely, experiment was carried out on grapefruit trees fertilized with composted sludge from Shiweitou Sewage Treatment Plant in Xiamen City of China, and a method was introduced of how to assess the environmental quality of grapefruit trees soil fertilized with sludge by Set Pair Analysis (SPA) model. The results showed that the soil in the surface layer (0-15cm) and the deeper layer (15-30cm) was less clean, and the environment of soil was not polluted. Thus it was feasible to use sludge as fruit fertilizer. The maximum service life of sludge for continuous land application was estimated by taking Cd as the limiting factor, which would provide scientific guide and technical support for safe land application of sludge.


Author(s):  
Pablo A. Lizana ◽  
Gustavo Vega-Fernadez ◽  
Alejandro Gomez-Bruton ◽  
Bárbara Leyton ◽  
Lydia Lera

Background: Prior to the COVID-19 pandemic, teachers were already reporting a low quality of life (QoL) perception, with a significant impact on mental and physical health due to various stress factors associated with work overload. The objective of this study was to evaluate the QoL impact on Chilean teachers before and during the COVID-19 pandemic. The analysis was performed following a longitudinal design on a sample of 63 Chilean teachers in pre-pandemic and COVID-19 pandemic timeframes. QoL perception, along with teachers’ sociodemographic data, was evaluated via the Short-Form 36 Health Survey (SF-36) questionnaire. Sociodemographic variables presented no significant variations in pre-pandemic and pandemic comparisons. QoL, however, showed a significant decrease during the pandemic compared to the pre-pandemic measurement (p < 0.01). In each gender, there were significant differences between pre-pandemic and pandemic timeframes, with a greater impact among women in the mental and physical component summary variables and seven of the eight QoL scales (p < 0.01). Between age categories, people under 45 presented significant differences (p < 0.05) between pre-pandemic and pandemic timeframes in all summary dimensions and measurements. In conclusion, Chilean teachers’ QoL perception has been affected by the COVID-19 pandemic. These findings could be related to work overload due to teleworking or feelings of uncertainty, loneliness, and fear that the pandemic and its associated confinements will worsen.


1993 ◽  
Vol 74 (3) ◽  
pp. 232-233
Author(s):  
E. E. Krasnoshchekova ◽  
K. T. Valeeva

Serum immunoglobulins (A, G, M) are usually quantified by radial gel immunodiffusion (RID). It is based on measuring the diameter of the precipitation ring formed when the serum is added to the wells cut out in the agar layer in which the monospecific antiserum is preliminarily dispersed. The gel plates are placed in a humid chamber for 24-48 hours. At the end of the incubation, the diameter of the precipitation ring is measured. Under standard experimental conditions, the diameter of the precipitation ring is directly proportional to the concentration of the studied immunoglobulin. The amount of immunoglobulins is determined by constructing a calibration graph. The results of determining the concentration of serum immunoglobulins by the RID method are issued only on the 3rd day of the study. Clinicians are not always satisfied with the timing of the results and the quality of the study, since the latter depends on many factors - thoroughness (conducting the experiment, the quality and thickness of the gel, the scale of the schedule, etc.)


2019 ◽  
pp. 16-27 ◽  
Author(s):  
I. V. Kuznetsova

Menstrual rhythm disorders and symptoms associated with the menstrual cycle are one of the main reasons for women to make an appointment with a gynecologist. After the endocrinopathies and organic substrates of menstrual irregularities are excluded, the doctor is faced with the difficult task to treat conditions that reflect the functional dysfunction of the body and its adaptation to high stress load. It is beyond argument that hormone therapy is the main resource of a gynecologist, but it is not always acceptable and does not solve all the problems of normalizing psychoneuroendocrine status. The dependence of functional disorders on environmental stress factors allows a doctor to use lifestyle correction, including rational nutrition and adding various micronutrients, in a program to improve the quality of life, eliminate psychoemotional symptoms and symptoms of autonomic dysfunction. In turn, the restoration of the adaptive reserve of the body becomes key to the recovery of menstrual function.


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