scholarly journals Influence of Endophytic Bacterium, Cellulosimicrobium sp. FRR2 on Plant Growth of Amaranthus campestris L. and Bacterial Survival at Adverse Environmental Conditions

2021 ◽  
Author(s):  
S. Sandhya ◽  
Ramalingam Radhakrishnan ◽  
Ramaraj Sathasivam ◽  
Muthukrishnan Arun ◽  
Gurusaravanan Packiaraj ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Endophytic Bacterium ◽  
Leafy Vegetables ◽  
Phylogenetic Study ◽  
Bacterial Survival ◽  
Acetic Acid Production ◽  
Antioxidants Activity ◽  
Green Leafy Vegetables ◽  
Beneficial Effects

The endophytic microorganisms are believed to be an important bio-resource for modern agriculture because of their beneficial effects on plant growth promotion, biocontrol, stress tolerance, and diseases resistance. This study was focused to know the beneficial effect of endophytic bacterium (FRR2) isolated from the roots of Ficus religiosa L. on Amaranthus campestris L. and their tolerance ability against salinity and heavy metals. The strain FRR2 was recognized as Cellulosimicrobium sp. by 16s rRNA sequencing and phylogenetic study. The bacterial isolate FRR2 showed salt (at 150 mM NaCl) and metal (at 150 µM CuSO4 and 100 µM ZnSO4) tolerance ability and significantly higher growth rate of Amaranthus campestris in a green leafy vegetable might be due to the nitrogen fixation, indole acetic acid production, amylase and protease activities. In addition, the endophyte FRR2 application slightly increased the antioxidants activity than their controls. The results of this study revealed that Cellulosimicrobium sp. strain FRR2 would be an effective endophyte to increase the growth of green leafy vegetables.

scholarly journals Potential for Plant Growth Promotion of Rhizobacteria Associated withSalicorniaGrowing in Tunisian Hypersaline Soils

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Francesca Mapelli ◽  
Ramona Marasco ◽  
Eleonora Rolli ◽  
Marta Barbato ◽  
Hanene Cherif ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Crop Production ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Growth Promotion ◽  
Arid Ecosystems ◽  
Acetic Acid Production ◽  
Halotolerant Bacteria ◽  
Microbiome Composition

Soil salinity and drought are among the environmental stresses that most severely affect plant growth and production around the world. In this study the rhizospheres ofSalicorniaplants and bulk soils were collected fromSebkhetandChotthypersaline ecosystems in Tunisia. Depiction of bacterial microbiome composition by Denaturing Gradient Gel Electrophoresis unveiled the occurrence of a high bacterial diversity associated withSalicorniaroot system. A large collection of 475 halophilic and halotolerant bacteria was established fromSalicorniarhizosphere and the surrounding bulk soil, and the bacteria were characterized for the resistance to temperature, osmotic and saline stresses, and plant growth promotion (PGP) features. TwentyHalomonasstrains showed resistance to a wide set of abiotic stresses and were able to perform different PGP activitiesin vitroat 5% NaCl, including ammonia and indole-3-acetic acid production, phosphate solubilisation, and potential nitrogen fixation. By using agfp-labelled strain it was possible to demonstrate thatHalomonasis capable of successfully colonisingSalicorniaroots in the laboratory conditions. Our results indicated that the culturable halophilic/halotolerant bacteria inhabiting salty and arid ecosystems have a potential to contribute to promoting plant growth under the harsh salinity and drought conditions. These halophilic/halotolerant strains could be exploited in biofertilizer formulates to sustain crop production in degraded and arid lands.

The recalcitrance of oxalate, nitrate and nitrites during the controlled lactic fermentation of commonly consumed green leafy vegetables

2015 ◽  
Vol 45 (2) ◽  
pp. 336-346 ◽  
Author(s):  
A. Jagannath ◽  
Manoranjan Kumar ◽  
P S Raju
Keyword(s):  
High Performance ◽  
Leafy Vegetables ◽  
Spontaneous Fermentation ◽  
Content Type ◽  
Lactic Fermentation ◽  
Green Leafy Vegetables ◽  
Beneficial Effects ◽  
Balanced Diet ◽  
Significant Difference ◽  
Nitrate And Nitrite

Purpose – Green leafy vegetables (GLVs) are important components of a balanced diet especially in developing countries where the major requirements of micronutrients are met. However, GLVs also contain significant amounts of oxalate, nitrate and nitrites, whose role in the human diet is constantly changing. The current study explored the behavior of nitrate, nitrites and oxalate in lactic-fermented GLVs with an intention to develop functional foods based on them. Design/methodology/approach – Selected strains of beneficial lactic acid bacteria were used for the controlled fermentation of GLV, while an identical portion was subjected to spontaneous fermentation. The nitrate and nitrites were monitored spectrophotometrically, while oxalate contents were quantified by both titrimetric and by high-performance liquid chromatography throughout the duration of fermentation. Findings – More than 90 per cent of individual constituents studied remained intact in the GLVs paste after the six-day controlled fermentation period. However, there was significant difference between the controlled and spontaneously fermented samples in terms of oxalate, nitrate and nitrite contents. Originality/value – Controlled lactic fermentation although superior in all other aspects may not be able to lower the anti-nutrients present. The advantages of spontaneous fermentation vis-à-vis controlled fermentation are discussed. The work will bring out the importance of the beneficial effects of GLVs and the effect of lactic fermentation.

scholarly journals Comparative Genomics Reveals Potential Mechanisms of Plant Beneficial Effects of a Novel Bamboo-Endophytic Bacterial Isolate Paraburkholderia sacchari Suichang626

2021 ◽  
Vol 12 ◽  
Author(s):  
Kai Wang ◽  
Ying Wu ◽  
Mengyuan Ye ◽  
Yifan Yang ◽  
Fred O. Asiegbu ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Plant Growth ◽  
Growth Promotion ◽  
Moso Bamboo ◽  
Associated Bacteria ◽  
Beneficial Effects ◽  
Genome Wide ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Genome Information

Plant-beneficial microbes have drawn wide attention due to their potential application as bio-control agents and bio-fertilizers. Moso bamboo, which is among the monocots with the highest growth rate, lives perennially with abundant microbes that may benefit annually growing crops. Genome information of moso bamboo associated bacteria remains underexplored. We isolated and identified a novel Paraburkholderia strain Suichang626 from moso bamboo roots. Growth promoting effects of Suichang626 on both moso bamboo and seedlings of the model dicot Arabidopsis thaliana were documented in laboratory conditions. To gain insight into the genetic basis of this growth promotion effect, we sequenced the genome of Suichang626. Evidenced by genome-wide phylogeny data, we propose that Suichang626 is a novel strain of Paraburkholderia sacchari. Gene homologs encoding biosynthesis of the plant growth-promoting chemicals, acetoin and 2,3-butanediol, were identified in the genome of Suichang626. Comparative genomics was further performed with plant-beneficial and plant/animal pathogenic species of Paraburkholderia and Burkholderia. Genes related to volatile organic compounds, nitrogen fixation, and auxin biosynthesis were discovered specifically in the plant growth-promoting species of both genera.

scholarly journals Isolation, Identification and Characterization of Endophytic Bacterium Rhizobium oryzihabitans sp. nov., from Rice Root with Biotechnological Potential in Agriculture

2020 ◽  
Vol 8 (4) ◽  
pp. 608 ◽  
Author(s):  
Juanjuan Zhao ◽  
Xia Zhao ◽  
Junru Wang ◽  
Qi Gong ◽  
Xiaoxia Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Endophytic Bacterium ◽  
Housekeeping Genes ◽  
Biotechnological Potential ◽  
Plant Growth Promoting ◽  
Comparative Genomes

A flagellate, rod–shaped bacterium designated strain M15T was isolated from rice roots. Phylogenetic analysis based on the sequences of the 16S rRNA, housekeeping genes and genomes showed that the isolate belonged to the genus Rhizobium, with the highest 16S rRNA similarity to Rhizobium radiobacter LMG140T (99.64%) and Rhizobium pusense NRCPB10T (99.36%), respectively. The complete genome of the strain M15T has a 59.28% G+C content, and the highest average nucleotide identity (ANI) and DNA-DNA relatedness (DDH) values were obtained with R. radiobacter LMG140T (88.11%, 54.80%), R. pusense NRCPB10T (86.00%, 53.00%) and R. nepotum 39/7T (88.80%, 49.80%), respectively. Plant growth-promoting characteristics tests showed that the strain M15T produced siderophore, 1–aminocyclopropane–1–carboxylate (ACC) deaminase and indole-3-acetic acid (IAA) and also produced some secondary metabolites according to the analysis of the comparative genomes. Based on the data mentioned above, we proposed that the strain M15T represented a novel species of the genus Rhizobium, named Rhizobium oryzihabitans sp. nov. The type strain is M15T (=JCM 32903T  = ACCC 60121T), and the strain M15T can be a novel biofertilizer Rhizobium to reduce the use of synthetic fertilizers for plant growth promotion.

scholarly journals Application of Seaweed Organic Components Increases Tolerance to Fe Deficiency in Tomato Plants

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 507
Author(s):  
Sandra Carrasco-Gil ◽  
Raúl Allende-Montalbán ◽  
Lourdes Hernández-Apaolaza ◽  
Juan J. Lucena
Keyword(s):  
Plant Growth ◽  
Organic Compounds ◽  
Growth Promotion ◽  
Abiotic Stress Tolerance ◽  
Nutrient Deficiency ◽  
Fe Deficiency ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Relevant Role ◽  
The Individual

The beneficial effects of seaweed extracts have been related to plant growth regulators present in seaweeds. However, algae extracts comprise other organic compounds such as phenols, mannitol, alginates, laminarins, and fucoidans that may have a relevant role regarding abiotic stress tolerance due to nutrient deficiency. Therefore, we evaluated the individual effect of these organic compounds in a range of concentrations on the mitigation of Fe deficiency in tomato plants. Germination and plant growth promotion, root morphology, chlorophyll content, and antioxidant activity were determined. Results showed that the lowest concentration of phenolics, laminarin, and fucose compounds contributed to increasing the tolerance to Fe deficiency in tomato plants.

scholarly journals Comparative Study of Agarophytes - Gracilaria edulis and Gelidiella acerosa as Biostimulant and Application of Agar for Water-holding in Soil and Plant Growth Promotion

2021 ◽  
Author(s):  
Saajida Sultaana Mahusook ◽  
F. Arockiya Aarthi Rajathi ◽  
H. Noorul Samsoon Maharifa ◽  
R. Sharmila
Keyword(s):  
Plant Growth ◽  
Plant Protection ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Plant Protection Products ◽  
Leafy Vegetables ◽  
Plant Tolerance ◽  
Agar Gels ◽  
Water Holding ◽  
Algal Polysaccharides

Background: Seaweeds and its derivatives are extensively used as biostimulants in horticulture and agriculture as a replacement for chemical fertilizers. G. edulis and G. acerosa are easily cultivable and economically important seaweeds. They are a rich source of phytohormones, amino acids, antibiotics, vitamins, micro, macro elements and agar. Such natural products have great demand and been commercialized these days to promote sustainable agriculture. Dried and finely powdered algal biomass is used directly as a biostimulant. Algal polysaccharides such as agar can be an innovative alternative to synthetic polymers used in horticulture as they contain active biostimulant compounds and also reported to hold water in the soil that aids plant growth with minimum water consumption than usually required. A. aritis being one of the most consumed leafy vegetables throughout the world can be harvested indoors with added nutrients and minimal water utilization.Methods: The field trial is a comparative evaluation of the two selected species of agarophytes for promoting plant (A. aritis) growth and the extracted agar tested for germination tests, bio-stimulatory property under water stress. Growth parameters were recorded after three weeks. The agarophytes were also qualitatively screened for phytochemicals and WD-XRF analysis.Result: The present work will be a supplementary contribution for assessing agarophytes with biostimulant properties and the characteristic agar gels that expand plant tolerance to abiotic stresses, thus constituting an alternative to synthetic plant protection products.

scholarly journals An endophyte Paenibacillus dendritiformis strain APL3 promotes Amaranthus polygonoides L. sprout growth and their extract inhibits food-borne pathogens

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Ramalingam Radhakrishnan ◽  
P Ajithkumar ◽  
Muthukrishnan Arun ◽  
Ramaraj Sathasivam ◽  
S Sandhya ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Bacterial Isolate ◽  
Antimicrobial Properties ◽  
Endophytic Bacterium ◽  
16S Rrna Gene Sequencing ◽  
Leafy Vegetables ◽  
Rrna Gene ◽  
Green Leafy Vegetables ◽  
Food Borne Pathogens ◽  
Food Borne

Green leafy vegetables are rich sources of antioxidants and minerals, which prevent food-borne pathogen infections during our diet. This study was aimed to isolate and identify the plant growth-promoting endophytic bacterium from several plant species to enhance the growth of Amaranthus polygonoides L. and their antimicrobial potential against food-borne pathogens. Seven endophytic bacterial isolates were tested on two Amaranthus species to identify the suitable beneficial bacterium. The antioxidants capacity and antimicrobial activity of bacterial isolate (APL3) treated plants were analyzed. The bacterial isolate, APL3 showed a significantly higher growth of A. polygonoides L. than other isolates. It was identified as Paenibacillus dendritiformis strain APL3 by 16S rRNA gene sequencing and phylogenetic analysis. The endophyte (APL3) treated A. polygonoides L. sprouts had higher antioxidants potentials and significantly inhibited the growth of Escherichia coli, Salmonella sp., Staphylococcus sp. and Pseudomonas sp. The results of the present study suggest that utilization of P. dendritiformis strain APL3 triggers the growth of A. polygonoides L. and induces metabolic changes in plants to improve their antimicrobial properties to prevent foodborne pathogens.

scholarly journals Impact of Pseudomonas spp. on Plant Growth, Lytic Enzymes and Secondary Metabolites Production

2021 ◽  
Vol 3 ◽  
Author(s):  
Rajesh R. Waghunde ◽  
Ambalal N. Sabalpara
Keyword(s):  
Plant Growth ◽  
Acid Production ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Root Weight ◽  
Bacterial Strains ◽  
Lytic Enzymes ◽  
Acetic Acid Production ◽  
Metabolites Production

Seven strains of Pseudomonas spp. were isolated from the south Gujarat region of India. Antifungal and bacterial activities of bacterial strains were evaluated against important plant pathogens in vitro, among them, PaRS was found most effective. The indole acetic acid production was recorded in all isolated Pseudomonas spp. Seed treatment with PaRS at 6 g/kg was significantly superior over other treatments for plant growth promotion (germination, shoot and root length, shoot and root weight, vigor index, and both shoot and root colonization) under standard roll towel method and pot conditions. The maximum siderophore production was observed in PaRS and medium production in PfRB, PaNS, and PfNC. PaNS and PaRS strains recorded strong HCN production but moderate production recorded in PaWP, PaWS, and PfNC. The maximum phosphate solubalization zone (22 mm) was found in PaRS. PaRS recorded maximum chitinase, ß-1,3-glucanase activity, hydrogen cyanide, and salicylic acid production as compared to other strains.

scholarly journals Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway

2021 ◽  
Vol 8 ◽  
Author(s):  
Twinkle Chaudhary ◽  
Rajesh Gera ◽  
Pratyoosh Shukla
Keyword(s):  
Plant Growth ◽  
Mung Bean ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Endophytic Bacterium ◽  
Fresh Weight ◽  
Root Endophyte ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

Plant growth-promoting rhizobacteria (PGPR) are root endophytic bacteria used for growth promotion, and they have broader applications in enhancing specific crop yield as a whole. In the present study, we have explored the potential of Rhizobium pusense MB-17a as an endophytic bacterium isolated from the roots of the mung bean (Vigna radiata) plant. Furthermore, this bacterium was sequenced and assembled to reveal its genomic potential associated with plant growth-promoting traits. Interestingly, the root endophyte R. pusense MB-17a showed all essential PGPR traits which were determined by biochemical and PGPR tests. It was noted that this root endophytic bacterium significantly produced siderophores, indole acetic acid (IAA), ammonia, and ACC deaminase and efficiently solubilized phosphate. The maximum IAA and ammonia produced were observed to be 110.5 and 81 μg/ml, respectively. Moreover, the PGPR potential of this endophytic bacterium was also confirmed by a pot experiment for mung bean (V. radiata), whose results show a substantial increase in the plant's fresh weight by 76.1% and dry weight by 76.5% on the 60th day after inoculation of R. pusense MB-17a. Also, there is a significant enhancement in the nodule number by 66.1% and nodule fresh weight by 162% at 45th day after inoculation with 100% field capacity after the inoculation of R. pusense MB-17a. Besides this, the functional genomic annotation of R. pusense MB-17a determined the presence of different proteins and transporters that are responsible for its stress tolerance and its plant growth-promoting properties. It was concluded that the unique presence of genes like rpoH, otsAB, and clpB enhances the symbiosis process during adverse conditions in this endophyte. Through Rapid Annotation using Subsystem Technology (RAST) analysis, the key genes involved in the production of siderophores, volatile compounds, indoles, nitrogenases, and amino acids were also predicted. In conclusion, the strain described in this study gives a novel idea of using such type of endophytes for improving plant growth-promoting traits under different stress conditions for sustainable agriculture.

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