Effects of Tapinanthus Globiferus Leaf Extract on Blood Glucose and Pancreatic Histology in Alloxanized and Normoglycemic Rats

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

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Effectiveness of Graptophyllum pictum (L.) Griff leaf extraction on blood glucose level in alloxan-induced Wistar rat

Food Research ◽

10.26656/fr.2017.4(s3).s12 ◽

2020 ◽

Vol 4 (S3) ◽

pp. 123-126

Author(s):

A. Leonoreza ◽

T. Excelinda ◽

J. Elnitiarta ◽

H.S. Heri-Nugroho ◽

M. Hendrianingtyas ◽

...

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Treatment Group ◽

Wistar Rats ◽

Leaf Extract ◽

Negative Control ◽

Normal Group ◽

Treatment Groups ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus is a metabolic disease that always increasing in prevalence. Excessive glucose intake and oxidative stress that can lead to damage to the pancreatic cell or insulin resistance, are such factors that influence the occurrence of diabetes mellitus. Graptophyllum pictum (L.) Griff leaf contains active metabolites such as flavonoid which able to decrease free radical levels. This study aimed to prove the effectiveness of Graptophyllum pictum (L.) Griff leaves extract on blood glucose levels in alloxan-induced Wistar rats. In this study, twenty-five male Wistar rats were divided into five groups. On day 0, the negative control and each treatment group injected with 125 mg/kg dose of alloxan and would be checked on 4th day. On the 4th day after the rats had diabetes, normal group (K-1) was given only standard feed, Negative control (K-2) was given only alloxan intraperitoneally on day 0, first treatment group (K-3) was given 50 mg/kg dose of Graptophyllum pictum (L.) Griff, second treatment group (K-4) was given 100 mg/kg dose of the leaf extract, and the third treatment group (K-5) was given the leaf 200 mg/kg dose of the leaf extract. On the 10th day, fasting blood glucose was taken from the rats. Post-test only group design was used to be the experimental design. There were significant differences in the treatment groups (K-3, K-4, and K-5) compared to the negative control group (K-2) after treatment. The mean of blood glucose levels decreased with the increased doses of Graptophyllum pictum (L.) Griff leaf extract treatment. The treatment groups (K-3, K-4, and K-5) were not significant compared to the normal group (K-1).

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UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

Jurnal Insan Farmasi Indonesia ◽

10.36387/jifi.v4i1.602 ◽

2021 ◽

Vol 4 (1) ◽

pp. 44-53

Author(s):

Andri Priyoherianto ◽

◽

Panji Ratih Suci ◽

Putri Rizki Cahya Fatimah ◽

Atik Nur Wijayanti

Keyword(s):

Blood Glucose ◽

Blood Sugar ◽

Water Hyacinth ◽

Leaf Extract ◽

Positive Control ◽

Negative Control ◽

Glucose Levels ◽

Normal Limits ◽

Blood Glucose Levels ◽

Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

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Effect of Pulutan (Urena lobata) Leaf Extract on Blood Glucose Level, Hemoglobin and Body Growth of Zebra Fish (Danio rerio) Exposed to Malathion

10.26538/tjnpr/v5i6.9 ◽

2021 ◽

Vol 5 (6) ◽

pp. 1034-1038

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Danio Rerio ◽

Leaf Extract ◽

Body Growth ◽

Zebra Fish

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Effects of aqueous leaf extract of azadirachta indica on some haematological parameters and blood glucose level in female rats

Nigerian Journal of Experimental and Clinical Biosciences ◽

10.4103/2348-0149.135731 ◽

2014 ◽

Vol 2 (1) ◽

pp. 54 ◽

Cited By ~ 2

Author(s):

EghosaE Iyare ◽

NancyN Obaji

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Azadirachta Indica ◽

Leaf Extract ◽

Female Rats ◽

Haematological Parameters ◽

Aqueous Leaf Extract

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EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT EFFECTS OF ETHANOLIC LEAF EXTRACT OF ERYTHRINA ABBYSINICA LAM, EX DC

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i8.24207 ◽

2018 ◽

Vol 11 (8) ◽

pp. 300

Author(s):

Kamadyaapa Davie Rexon ◽

Gondwe Mavuto Masopera ◽

Shauli Mathulo ◽

Sewani Rusike Constance ◽

Nkeh Chungag Benedicta

Keyword(s):

Blood Glucose ◽

Leaf Extract ◽

Antioxidant Properties ◽

Diabetic Rats ◽

Control Group ◽

Oral Glucose ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Ethanolic Leaf Extract ◽

Antioxidant Effects

Objective: This study was conducted to scientifically evaluate the antidiabetic and antioxidant effects of ethanolic leaf extract of Erythrina abbysinica (EEA).Methods: Acute and sub-chronic effects of EEA at 100, 200, and 400 mg/kg/bwt and glibenclamide (GL) at 5 mg/kg/bwt. were evaluated in both normal and streptozotocin (STZ)-induced diabetic male Wistar rats (250–300 g). The acute studies were performed using oral glucose tolerance test (OGTT). In sub-chronic studies, animals were orally administered with EEA and GL daily for 6 w. Brine shrimp assay was used to determine the toxicity of EEA. 1, 1-diphenyl-2-picrylhydrazyl, ferric reducing capacity of plasma, and thiobarbituric acid reactive substances assays were used to determine antioxidant properties of EEA.Results: Following OGTT, EEA significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in both normal and STZ-induced diabetic rats when compared with positive and negative control counterparts at all-time points, whereas GL significantly (p<0.05) decreased blood glucose only in normal rats but not in diabetic rats. Daily, oral administration of EEA for 6 w significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in STZ-induced diabetic rats when compared with the diabetic control group. EEA revealed weak toxicity with a lethal concentration50 value of 997 μg/ml). Furthermore, EEA showed significant free radical scavenging, total antioxidant, and anti-lipid peroxidative capacities.Conclusion: The study has shed more light on the scientific basis for the use of E. abbysinica in management of diabetes in some communities of Eastern Cape of South Africa.

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Use of Gymnema sylvestre leaf extract in the control of blood glucose in insulin-dependent diabetes mellitus

Journal of Ethnopharmacology ◽

10.1016/0378-8741(90)90107-5 ◽

1990 ◽

Vol 30 (3) ◽

pp. 281-294 ◽

Cited By ~ 118

Author(s):

E.R.B. Shanmugasundaram ◽

G. Rajeswari ◽

K. Baskaran ◽

B.R.Rajesh Kumar ◽

K.Radha Shanmugasundaram ◽

...

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Leaf Extract ◽

Insulin Dependent Diabetes Mellitus ◽

Insulin Dependent Diabetes ◽

Dependent Diabetes Mellitus ◽

Gymnema Sylvestre ◽

Insulin Dependent

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Mulberry leaf extract improves glycaemic response and insulaemic response to sucrose in healthy subjects: results of a randomized, double blind, placebo-controlled study

Nutrition & Metabolism ◽

10.1186/s12986-021-00571-2 ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Pariyarath Sangeetha Thondre ◽

Helen Lightowler ◽

Lis Ahlstrom ◽

Andrew Gallagher

Keyword(s):

Blood Glucose ◽

Leaf Extract ◽

Lifestyle Changes ◽

Glycaemic Response ◽

Double Blind ◽

Glucose Levels ◽

Mulberry Leaf ◽

Blood Glucose Levels ◽

Mulberry Leaf Extract ◽

White Mulberry

Abstract Background There are many benefits of maintaining healthy blood glucose levels, and studies have shown that lifestyle changes such as changes to diet can successfully restore normoglycaemia in participants with dysglycaemia. Significant health-related lifestyle changes are often difficult to implement and functional ingredients that can reduce glycaemic and insulaemic responses may help at risk populations. The aim of this study was to investigate whether a mulberry leaf extract could lower the glycaemic and insulinaemic responses to 75 g sucrose in healthy individuals. Methods A double-blind, randomised, placebo-controlled, crossover design trial was conducted by the Oxford Brookes Centre for Nutrition and Health. Thirty-eight participants were recruited into the trial and, after an overnight fast, were given 75 g sucrose + white mulberry leaf extract, or 75 g sucrose alone. Capillary blood samples were collected at 15-min intervals in the first hour and at 30-min intervals over the second hour to determine glucose and plasma insulin levels. Data analysis was conducted using a paired samples T test or a Wilcoxon signed rank test. Results The addition of mulberry leaf extract to sucrose resulted in a significantly lower glycaemic response and insulinaemic response compared to a matched placebo (sucrose alone). The change in blood glucose measurements were significantly lower at 15 min (p < 0.001), 30 min (p < 0.001), 45 min (p = 0.008), and 120 min (p < 0.001) and plasma insulin measurements were significantly lower at 15 min (p < 0.001), 30 min (p < 0.001), 45 min (p < 0.001), 60 min (p = 0.001) and 120 min (p < 0.001). The glucose iAUC (− 42%, p = 0.001), insulin iAUC (− 40%, p < 0.001), peak glucose (− 40.0%, p < 0.001) and peak insulin (− 41%, p < 0.001) from baseline were significantly lower for white mulberry leaf extract compared with the placebo. White mulberry leaf extract was well tolerated and there were no reported adverse events. Conclusions Mulberry leaf extract can be used as part of lifestyle changes that may lead to healthy blood glucose levels. Trial registration: ISRCTN99601810 (23 October 2020, retrospectively registered)

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