scholarly journals Determination of Criteria and Drought Tolerance of Prope-Legitimate Cocoa Seed Through Polyethylene Glycol Induction

2020 ◽  
Vol 36 (2) ◽  
pp. 120-130
Author(s):  
Maera Zasari ◽  
Agung Wahyu Susuilo ◽  
Ade Wachjar ◽  
Sudarsono Sudarsono
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Selection Criteria ◽  
Plant Materials ◽  
Peg 6000 ◽  
Drought Tolerant ◽  
Germination Ability ◽  
Main Plot ◽  
Susceptible Group

The availability of drought tolerant rootstock becomes one of solution to obtain tolerant cocoa clone at drought condition. The production of rootstock is easier and faster by using prope-legitimate seeds as plant materials. Seed germination selection phase can accelerate the obtainment of tolerant cocoa rootstocks. The tolerance trait of prope-legitimate seeds can be determined by seed germination on media induced by polyethylene glycol (PEG) solution. To determine the tolerant seeds, it is effective by using a specific character as selection criteria. This study aimed to obtain criteria and selection character to determine droughttolerant characteristic of prope-legitimate seed through PEG 6000 induction in the germination phase. The research was conducted at the Agronomy Laboratoryof the Indonesian Coffee and Cocoa Research Institute, Jember, East Java, Indonesia, used split plot design with five replications as experimental design. The main plot was solution of 0% and 6% PEG 6000, while the subplot were prope-legitimate seeds of 13 cocoa clones. The result showed that germination ability and sprout fresh weight were suitable as selection criteria to determine tolerant characteristic of prope-legitimate seeds to drought stress. Based on the value of  tress susceptible index and clustering analysis, the tested prope-legitimate seeds were divided into three groups. The group of tolerant genotype consisted of Sca 06, while the group of medium tolerant were ICCRI 03, Sulawesi 02, KW 641, TSH 858, KEE 02, Sulawesi 03, and KW 617. The susceptible group consisted of six genotypes, namely ICS 60, KKM 22, KW 516, Sulawesi 01, and MCC 02. 

scholarly journals Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

2021 ◽  
Vol 8 (3) ◽  
pp. 57-66
Author(s):  
Ratiba Bousba ◽  
Rabah Bounar ◽  
Narimene Sedrati ◽  
Randa Lekhal ◽  
Chourouk Hamla ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Durum Wheat ◽  
Crop Production ◽  
Germination Rate ◽  
Inhibitory Effect ◽  
Wheat Genotype ◽  
Peg 6000 ◽  
Germination Parameters

Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was generated by polyethylene glycol (PEG) 6000 and mannitol, related to drought stress and growth of Waha durum wheat genotype. Two tests were carried out in a growth chamber; the first comprises seed germination into Petri dishes in the presence of different concentrations of the two osmoticums (0, 5, 10, 15 and 20 % of PEG6000 and mannitol). The second test was carried out in nutrient solution BD medium. Our results shows that Both PEG -6000 and mannitol reduced germination. Therefore, a rapid increase was observed in the rate of germination both for the control plants and the plants subjected to a concentration of 5 g/L and 10 g/L and changes in proportion to the time. For the concentration of 15 g/L and 20 g/L, this phase is very short, which explains the reduced germination rate due to the inhibitory effect of the two osmoticums on germination. In this study, PEG-6000 treatments resulted in an increase of some proteins and a decrease of others. Waha displayed 12 bands for control plants, 40 bands for PEG-6000 stressed plants (all treatments) and 35 bands for mannitol treatments.

Prope legitimate rootstocks determine the selection criteria for drought-tolerant cocoa

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Maera Zasari ◽  
Ade Wachjar ◽  
Agung Wahyu Susilo ◽  
Sudarsono Sudarsono
Keyword(s):  
Clustering Analysis ◽  
Selection Criteria ◽  
Soil Moisture Content ◽  
Fresh Weight ◽  
Plant Materials ◽  
Drought Tolerant ◽  
Main Plot ◽  
Cocoa Seedlings ◽  
Selection Of ◽  
Plot Design

Abstract. Zasari M, Wachar A, Susilo AW, Sudarsono. 2020. Prope legitimate rootstocks determine the selection criteria for drought-tolerant cocoa. Biodiversitas 21: 4067-4075. Drought tolerant cocoa seedlings are needed for the success of cocoa cultivation under drought conditions. Prope legitimate rootstock is feasible to be used in the selection of drought-tolerant cocoa seedlings. This objective of this study was to determine the selection criteria and tolerance of prope legitimate cocoa rootstocks in response to drought stress. This study was conducted in the greenhouse of Kaliwining experimental garden, Indonesian Center for Coffee and Cocoa Research, Jember, Indonesia. Plant materials were arranged in a split-plot design with 2 factors and 6 replications. The main plot was the soil moisture content, i.e 100%, and 25%, while the subplots were 13 prope legitimate cocoa rootstocks. The result showed that root fresh weight was the best characters for the selection of drought tolerance in prope legitimate cocoa rootstock. Based on the value of stress susceptible index and clustering analysis divided the tested prope legitimate rootstock into two groups. The group for tolerant genotype was consisted of ICCRI 03, ICS 60, TSH 858, KKM 22, KW 641, KW 516, and MCC 02, while the sensitive genotype group was consisted of Sul 01, Sul 03, Sca 06, KEE 02, KW 617, and Sul 02.

Effect of Priming Techniques on Seed Germination Characteristics of C. Maxima Duch

2011 ◽  
Vol 474-476 ◽  
pp. 36-39 ◽  
Author(s):  
Yong Dong Sun ◽  
Xin Zheng Li ◽  
He Lian Yang ◽  
Li Sun
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Seed Priming ◽  
Distilled Water ◽  
Nacl Solution ◽  
Peg 6000 ◽  
Germination Process ◽  
Germination Characteristics ◽  
Successful Technique

The present study was conducted to investigate the effect of seed priming techniques on germination characteristics of C. maxima Duch. cultivar (Beiguan). Treatments were combinations of 3 levels of priming (distilled water, NaCl and PEG6000) and non-priming (control) with 3 replications. Concentrations of NaCl solution were 50, 100, 150, 200 mmol•L-1, and polyethylene glycol (PEG) 6000 were 10%, 20%, 30%, 40%, respectively. Seeds were primed using the above priming materials for 24 hours at 20°C in the dark, respectively. The results showed that different priming techniques could have various effects on germination of Beiguan seeds. Hydropriming (distilled water), NaCl priming and PEG6000 priming (10%) all improved the germination characteristics of Beiguan, compared to the control. NaCl priming was more effective than hydropriming and PEG6000 priming and was the most successful technique in this study. These findings indicated that seed priming techniques could accelerate germination process and were simple and cheap, we should propose these methods to farmers.

scholarly journals The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage

Jurnal Wasian ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 133-143
Author(s):  
Iskandar Siregar ◽  
◽  
Riki Ramdhani ◽  
Evayusvita Rustam ◽  
Dede Sudrajat ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Seed Viability ◽  
Germination Capacity ◽  
Peg 6000 ◽  
Vigor Index ◽  
Fine Bubble ◽  
Initial Seed ◽  
Environmental Air

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

scholarly journals Pattern of Capsule and Seed Development in Sesame (Sesamum indicum L.) as Influenced by Seed Priming

2020 ◽  
pp. 1-7
Author(s):  
Utpal Biswas ◽  
Rupa Das ◽  
Amitava Dutta
Keyword(s):  
Polyethylene Glycol ◽  
Seed Development ◽  
Seed Weight ◽  
Seed Priming ◽  
Quality Parameters ◽  
Steady Increase ◽  
Peg 6000 ◽  
Yield And Quality ◽  
Main Plot ◽  
Days After Anthesis

Aim: To study the influence of seed priming on the pattern of capsule and seed development in sesame. Place and Duration of Study: The field experiment was conducted during the pre kharif seasons of 2017-2018 and 2018-2019 in sesame variety Savitri at AB Block farm, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal, India. Methodology: Experiment was laid out in split plot design with 3 replications. Ten schedules of seed priming viz T1 (KNO3 @ 10 mM), T2 (KNO3 @ 20 mM), T3 (KNO3 @ 50 mM), T4 (KH2PO4 @ 50 mM), T5 (KH2PO4 @ 100 mM),T6 (KH2PO4 @ 200 mM), T7 [Polyethylene glycol (PEG) 6000 @ -0.4 MPa], T8 [Polyethylene glycol (PEG) 6000 @ -0.3 MPa], T9 [Polyethylene glycol (PEG) 6000 @ -0.2 MPa], T10 Distilled water (Hydro priming) along with control T11 (Dry seed) were taken as main plot treatment and stage of harvest was considered as sub plot treatment. The pattern of capsule and seed development was studied at 10 days after anthesis (DAA), 20 days after anthesis (DAA), 30 days after anthesis (DAA), 40 days after anthesis (DAA) and 50 days after anthesis (DAA) interval. Ten plants from each replication and in each treatment were selected at random to record data on morphological and physiological characters. Results: Fresh capsule length, fresh capsule breadth, fresh capsule weight, fresh seed weight and dry seed weight showed a steady increase up to 40 days after anthesis (DAA) then decreased slowly up to maturity. Conclusion: Considering seed yield and quality parameters, T7 [Polyethylene glycol (PEG) 6000 @ -0.4 MPa] and T9 [Polyethylene glycol (PEG) 6000 @ -0.2 MPa] appears to be ideal among the treatments for quality seed production in sesame.

scholarly journals OPTIMASI PERLAKUAN POLYETHYLENE GLIKOL (PEG) 6000 TERHADAP ISOLASI AGAROSA RUMPUT LAUT Glacilaria sp.

2017 ◽  
Vol 4 (2) ◽  
pp. 115
Author(s):  
Siti Martinah ◽  
RTM Sutamihardja ◽  
Lilis Sugiarti
Keyword(s):  
Polyethylene Glycol ◽  
Key Words ◽  
Neutral Condition ◽  
High Yield ◽  
Acid Base ◽  
Peg 6000 ◽  
Parameter Test ◽  
Galactose Content

Optimation of Polyethylene Glycol (PEG) 6000 Treatment on Isolation of Agarose Seaweeds of Glacilaria sp         Isolation of agarose from seaweeds (Glacilaria sp) had been done in acid, base, and neutral condition by using polyethylene glycol (PEG) method. Glacilaria sp, Seaweeds that used in this research was cultured by farmer in Muara Gembong, Bekasi. It used PEG with concentration 10%, 15%, 20%, 25%, & 30%. The scope of this research ware determination of seaweeds, seaweeds water content, isolation of agarose from seaweeds, and quality test of agarose. Parameter test of agarose quality include  the strength of gel, the content of sulfat, measurement of galactose content and its proksimat. The high yield was 61,27% r  in PEG 30%. The best quality of agarose was resulted in PEG 20% because of the highest strength of gel was 379,04 g/cm2.The lowest sulfat concentration was 1,33% and galactose was 17,39%.Key words: polyethylene glycol, PEG 6000, agarose, seaweeds, glacilaria sp ABSTRAK         Isolasi agarosa dari rumput laut Glacilaria sp telah dilakukan dalam suasana asam, basa dan netral dengan metode polyethylene glikol (PEG). Rumput lauat yang digunakan adalah Gracilaria sp yang dibudidayakan oleh petani di Muara Gembong, Bekasi dengan perlakuan konsentrasi PEG 10%, 15%, 20%, 25% dan 30%. Ruang lingkup penelitian ini meliputi uji determinasi rumput laut, penetapan kadar air rumput laut, isolasi agarosa dari rumput laut, serta uji kualitas agarosa. Parameter pengujian kualitas agarosa terdiri dari kekuatan gel, kandungan sulfat, dan pengukuran kandungan galaktosa serta proksimatnya. Isolasi agarosa perlakuan konsentrasi polyethylene Glikol (PEG) 6000 pada rumput laut Glacilaria sp diperoleh rendemen paling tinggi terdapat pada perlakuan PEG 30% yaitu sebesar 61,27%. Akan tetapi untuk kualitas agarosa yang paling baik terdapat pada perlakuan konsentrasi PEG 20% karena diperoleh kekuatan gel paling tinggi sebesar 379,04 g/cm2. Kadar sulfat yang paling rendah sebesar 1,33% dan kadar galaktosa sebesar 17,39%.Kata Kunci: polyethylene glikol, PEG 6000, agarosa, rumput laut, glacilaria sp

scholarly journals Concentration effect of polyethylene glycol in evaluation of grain legumes for drought tolerance

2020 ◽  
Vol 27 (2) ◽  
Author(s):  
Nadiia Vus ◽  
Antonina Vasylenko ◽  
Vyacheslav Lutenko ◽  
Lyubov Kobyzeva ◽  
Olha Bezuhla ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Drought Tolerance ◽  
Genetic Resource ◽  
Grain Legumes ◽  
Osmotic Concentration ◽  
Peg 6000 ◽  
Drought Tolerant ◽  
Pea Seeds ◽  
Maximum Differentiation ◽  
Selection Of

The article covers selection of differentiating concentrations of PEG-6000 for assessing genetic resource collections of pea, chickpea and lentil. The germinability of 4 accessions of each crop in 5, 10, 15, 20 and 25% PEG-6000 solutions was evaluated. The results showed that 25% PEG-6000 completely inhibited growth processes in all the crops; 5 and 10% PEG-6000 did not affect the germinability of lentil seeds; and the maximum differentiation was observed at an osmotic concentration of 20%. In chickpea, there were no seedlings even in 20% PEG-6000. In 15% PEG-6000, seeds of drought-tolerant accessions UD0500022 and Dnіprovskyi Vysokoroslyi only sprouted; and 5 and 10% solutions had the maximum differentiating effect. Pea germination in PEG-6000 solutions of different concentrations demonstrated that only one accession could germinate in 20 and 15% solutions. Two accessions gave seedlings in 10% PEG-6000, and 5% solution had almost no effect on the germinability of pea seeds.

scholarly journals Stevia rebaudiana (Bert) Bertoni: influence of osmotic stress and seed priming on seed germination under laboratory conditions

2017 ◽  
Vol 39 (3) ◽  
pp. 379 ◽  
Author(s):  
Keila Regina Hossa ◽  
José Walter Pedroza Carneiro ◽  
Terezinha Aparecida Guedes ◽  
Alessandro Lucca Braccini
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Stevia Rebaudiana ◽  
Seed Priming ◽  
Initial Time ◽  
Peg 6000 ◽  
Bedding Plants ◽  
Normal Seedling ◽  
First Results

 The foremost factor necessary for plant growers cultivating large acreages of Stevia rebaudiana (Bert) Bertoni is the production of qualitative bedding plants. The objective of this study was to evaluate the influence of osmotic-priming on the uniformity of seed germination. First, we evaluated the percentage of normal seedlings from two seed samples harvested in 2011 and 2012. The seeds harvested in 2012 produced 71.4% normal seedlings and thus they were used in the next experiments. The seeds were subjected to osmotic stress using five concentrations of polyethylene glycol (PEG-6000) at -0.2, -0.4, -0.6, -0.8, and -1.0 MPa in contrast with distilled water. Based on these first results, only -0.8 and -1.0 MPa were evaluated in the third experiment. The seeds were immersed in both concentrations of polyethylene glycol (PEG-6000) for imbibing at 20ºC for four, five, six, and seven days. Thereafter, we evaluated the time to the first normal seedling (Ti), time to the last normal seedling (Tf), percentage normal seedlings at the initial time (Pi) and percentage of normal seedlings at the end of every treatment (Pf). Osmotic priming increased the percentage of normal seedlings of the Stevia rebaudiana and reduced the time to the first and last germination events. 

scholarly journals In Vitro Screening of Sugarcane Cultivars (Saccharum spp. Hybrids) for Tolerance to Polyethylene Glycol-Induced Water Stress

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 598
Author(s):  
César A. Hernández-Pérez ◽  
Fernando Carlos Gómez-Merino ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
In Vitro Screening ◽  
Sugarcane Crop ◽  
Drought Tolerant ◽  
Solid Culture Medium ◽  
Use Efficiency ◽  
Semi Solid

Water stress caused by drought affects the productivity of the sugarcane crop. A breeding alternative is the selection of drought-tolerant sugarcane cultivars. The objective of this study was the in vitro screening of cultivars tolerant to water stress using polyethylene glycol (PEG) as a stressing agent. Cultivars (cv) Mex 69-290, CP 72-2086, Mex 79-431 and MOTZMex 92-207 were subjected to different concentrations of PEG 6000 (0, 3, 6 and 9% w/v) using Murashige and Skoog semi-solid culture medium. At 30 days of culture, different developmental variables and dry matter (DM), total protein (TP), proline (Pr) and glycine-betaine (GB) contents were evaluated. The results showed reduced development in cv CP 72-2086, Mex 79-431 and MOTZMex 92-207 with increasing PEG concentration. The cv Mex 69-290 showed tolerance to osmotic stress of −0.45 MPa using 3% PEG. Overall, TP content decreased with increasing PEG concentration, while DM, Pr and GB contents rose with increasing PEG concentration in all evaluated cultivars. Our results suggest that cv Mex 69-290 has a slight tolerance to water stress and could be used for rainfed cultivation with low rainfall or reduced irrigation for better water use efficiency. In conclusion, the in vitro screening technique of cultivars tolerant to PEG-induced water stress is an alternative for early determination of drought stress in sugarcane.

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