scholarly journals OPTIMASI PERLAKUAN POLYETHYLENE GLIKOL (PEG) 6000 TERHADAP ISOLASI AGAROSA RUMPUT LAUT Glacilaria sp.

2017 ◽  
Vol 4 (2) ◽  
pp. 115
Author(s):  
Siti Martinah ◽  
RTM Sutamihardja ◽  
Lilis Sugiarti
Keyword(s):  
Polyethylene Glycol ◽  
Key Words ◽  
Neutral Condition ◽  
High Yield ◽  
Acid Base ◽  
Peg 6000 ◽  
Parameter Test ◽  
Galactose Content

Optimation of Polyethylene Glycol (PEG) 6000 Treatment on Isolation of Agarose Seaweeds of Glacilaria sp         Isolation of agarose from seaweeds (Glacilaria sp) had been done in acid, base, and neutral condition by using polyethylene glycol (PEG) method. Glacilaria sp, Seaweeds that used in this research was cultured by farmer in Muara Gembong, Bekasi. It used PEG with concentration 10%, 15%, 20%, 25%, & 30%. The scope of this research ware determination of seaweeds, seaweeds water content, isolation of agarose from seaweeds, and quality test of agarose. Parameter test of agarose quality include  the strength of gel, the content of sulfat, measurement of galactose content and its proksimat. The high yield was 61,27% r  in PEG 30%. The best quality of agarose was resulted in PEG 20% because of the highest strength of gel was 379,04 g/cm2.The lowest sulfat concentration was 1,33% and galactose was 17,39%.Key words: polyethylene glycol, PEG 6000, agarose, seaweeds, glacilaria sp ABSTRAK         Isolasi agarosa dari rumput laut Glacilaria sp telah dilakukan dalam suasana asam, basa dan netral dengan metode polyethylene glikol (PEG). Rumput lauat yang digunakan adalah Gracilaria sp yang dibudidayakan oleh petani di Muara Gembong, Bekasi dengan perlakuan konsentrasi PEG 10%, 15%, 20%, 25% dan 30%. Ruang lingkup penelitian ini meliputi uji determinasi rumput laut, penetapan kadar air rumput laut, isolasi agarosa dari rumput laut, serta uji kualitas agarosa. Parameter pengujian kualitas agarosa terdiri dari kekuatan gel, kandungan sulfat, dan pengukuran kandungan galaktosa serta proksimatnya. Isolasi agarosa perlakuan konsentrasi polyethylene Glikol (PEG) 6000 pada rumput laut Glacilaria sp diperoleh rendemen paling tinggi terdapat pada perlakuan PEG 30% yaitu sebesar 61,27%. Akan tetapi untuk kualitas agarosa yang paling baik terdapat pada perlakuan konsentrasi PEG 20% karena diperoleh kekuatan gel paling tinggi sebesar 379,04 g/cm2. Kadar sulfat yang paling rendah sebesar 1,33% dan kadar galaktosa sebesar 17,39%.Kata Kunci: polyethylene glikol, PEG 6000, agarosa, rumput laut, glacilaria sp

scholarly journals Determination of the Effect of Humic Acid on Growth and Development Parameters of Parsley (Petroselinum sativum Hoffm.) Grown in Boron Soil

2018 ◽  
Vol 47 (1) ◽  
pp. 183-193 ◽  
Author(s):  
Tuncay TURSUN ◽  
Sener AKINCI ◽  
Esin BOZKURT
Keyword(s):  
Humic Acid ◽  
Growth Parameters ◽  
Dry Weight ◽  
High Yield ◽  
Yield And Quality ◽  
Icp Ms ◽  
Growth Analyses ◽  
Nutrient Solutions

Boron is an important micronutrient, required for all plant growth, and critical for high yield and quality of crops. The aim of the present research was to determine the effects of boron on pot-grown parsley (Petroselinum sativum Hoffm.). The experimental design consisted of four treatments using Hoagland-Arnon (1950) nutrient solutions with two different boron concentrations (B1 - 15 ppm and B2 - 150 ppm), each with and without 10 ml humic acid addition (HB1 and HB2), and controls with full strength Hoagland-Arnon solutions. Growth analyses of the parsley revealed that 15 ppm boron application caused an increase in root length leaf fresh and dry weight root fresh and dry weight and leaf area compared to control values. 150 ppm B (B2) concentration decreased all growth parameters compared to controls. The two humic acid treatments (HB1 and HB2) did not increase any of those growth parameters either in controls (C) or in the two boron (B1 and B2) concentrations. Analysis by (ICP-MS) revealed that B content in the leaves increased gradually in B1 and B2, as well as in both humic treatments where in HB2 it increased to 99.38% compared to B1. In the leaves, Mn, Zn and Fe contents behaved the same as B, increasing in all treatments, with the amounts in HB2 being significantly greater than in C, B1 and B2 leaves.

scholarly journals Determination of NABE in urine of high-yield dairy cows in early lactation in conditions of moderate heat stress

2010 ◽  
Vol 64 (5-6) ◽  
pp. 349-358
Author(s):  
Natalija Fratric ◽  
Ivan Vujanac ◽  
Horea Samanc ◽  
Danijela Kirovski ◽  
Dragan Gvozdic ◽  
...  
Keyword(s):  
Heat Stress ◽  
Dairy Cows ◽  
High Yield ◽  
Early Lactation ◽  
Acid Base Balance ◽  
Acid Base ◽  
Urine Ph ◽  
Base Balance ◽  
Daily Milk Production

The work presents the results of investigations of the effect of moderate heat stress on the acidobasal status in high-yield dairy cows in early lactation. Improving performance in high-yield dairy cows increases their inclination toward metabolic disorders. The most likely to be affected is the acid-base balance, in particular when cows are exposed to heat stress. Investigations so far have shown that the taking of urine samples and their analysis is the best and fastest way to diagnose disorders in the acid-base balance. Investigations were carried out on 7 clinically healthy cows of the Holstein- Friesian breed in the phase of early lactation, 30 to 40 days (on days 30, 33 and 40 ) following calving during the summer period, during the month of July, when there were significant variations in daily and nightly temperatures. The cows were in the second and fourth lactation, the annual milk yield was 8000 L milk per cow. The average daily milk production in the early phase of lactation ranged from 35 to 40 L. The cows were fed mixed rations (TMR) twice daily. Lucerne hay in limited quantities was given to the cows prior to the mixed feed ration. The ration for this animal category was optimized on the grounds of the daily milk production. The balance of cations and anions in the feed ration stood at 95 mEq/kg DM. The results clearly demonstrate the cows' response to moderate heat stress through the defense parameters in urine (urine pH, NABE (net-acid-base-excretion), acids, bases, ammonium ion (NH4). The determination of the kidney NABE yields more correct data than the urine pH on acidotic conditions. The results of examinations of the urine pH do not show any digressions from physiological values and are approximately the same in all cows during the investigated periods. Normal NABE values are from 100-200 mmol/L. Burdening with acid products results in a NABE range from 0-100mmol/L, and metabolic acidosis results in NAB<0 mmol/L. NABE in the cows in this experiment revealed that the organism is burdened by acid metabolic products and it stood at 58.28?27.96 mmol/L on day 30, 69.28?29.89 mmol/L on day 33, and 60.60?26.88mmol/L on day 40. Consequently, it seems that NABE values are decreased in cases of acidosis due to stepped up H+ excretion and stepped up bicarbonate reabsorption.

scholarly journals Assessment of the Suitability of Aromatic and High-Bitter Hop Varieties (Humulus lupulus L.) for Beer Production in the Conditions of the Małopolska Vistula Gorge Region

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 104
Author(s):  
Barbara Sawicka ◽  
Mateusz Śpiewak ◽  
Anna Kiełtyka-Dadasiewicz ◽  
Dominika Skiba ◽  
Bernadetta Bienia ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Essential Oils ◽  
Humulus Lupulus ◽  
High Yield ◽  
Brewing Industry ◽  
Monoterpene Hydrocarbons ◽  
Humulus Lupulus L ◽  
Beer Production

The aim of the study was to assess the yield of cones and the quality of six hop varieties (“Iunga”, “Lubelski”, “Sybilla”, “Magnum”, “Lomik”, “Marynka”) in terms of their suitability for beer production, under the conditions of the Małopolska Vistula Gorge Region (21°53′ E; 51°16′ N) (2015–2017). The scope of the research included the determination of the yield of cones and their physicochemical properties, as well as determination of the contents of hop resins and essential oils. In bitter varieties, the ratio of alpha-acids to beta-acids was stable, while, in aromatic varieties, it was variable. In the essential oils of the studied hop varieties, compounds with myrcene and α-humulene were identified and dominated the profiles. “Iunga”, “Sybilla”, “Lubelski”, and “Lomik” were rich in monoterpene hydrocarbons, mainly myrcene, while there was a bit more α-humulene in the “Marynka” variety. “Magnum” was characterized by an even distribution of monoterpenes and sesquiterpenes. The “Magnum variety turned out the most useful for the brewing industry in this region. The Małopolska Vistula Gorge Region is a region where hops not only achieve a high yield of cones, but also good-quality. The bitterness and aroma content of the hops in this region is high.

scholarly journals Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

2021 ◽  
Vol 8 (3) ◽  
pp. 57-66
Author(s):  
Ratiba Bousba ◽  
Rabah Bounar ◽  
Narimene Sedrati ◽  
Randa Lekhal ◽  
Chourouk Hamla ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Durum Wheat ◽  
Crop Production ◽  
Germination Rate ◽  
Inhibitory Effect ◽  
Wheat Genotype ◽  
Peg 6000 ◽  
Germination Parameters

Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was generated by polyethylene glycol (PEG) 6000 and mannitol, related to drought stress and growth of Waha durum wheat genotype. Two tests were carried out in a growth chamber; the first comprises seed germination into Petri dishes in the presence of different concentrations of the two osmoticums (0, 5, 10, 15 and 20 % of PEG6000 and mannitol). The second test was carried out in nutrient solution BD medium. Our results shows that Both PEG -6000 and mannitol reduced germination. Therefore, a rapid increase was observed in the rate of germination both for the control plants and the plants subjected to a concentration of 5 g/L and 10 g/L and changes in proportion to the time. For the concentration of 15 g/L and 20 g/L, this phase is very short, which explains the reduced germination rate due to the inhibitory effect of the two osmoticums on germination. In this study, PEG-6000 treatments resulted in an increase of some proteins and a decrease of others. Waha displayed 12 bands for control plants, 40 bands for PEG-6000 stressed plants (all treatments) and 35 bands for mannitol treatments.

scholarly journals Determination of Criteria and Drought Tolerance of Prope-Legitimate Cocoa Seed Through Polyethylene Glycol Induction

2020 ◽  
Vol 36 (2) ◽  
pp. 120-130
Author(s):  
Maera Zasari ◽  
Agung Wahyu Susuilo ◽  
Ade Wachjar ◽  
Sudarsono Sudarsono
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Selection Criteria ◽  
Plant Materials ◽  
Peg 6000 ◽  
Drought Tolerant ◽  
Germination Ability ◽  
Main Plot ◽  
Susceptible Group

The availability of drought tolerant rootstock becomes one of solution to obtain tolerant cocoa clone at drought condition. The production of rootstock is easier and faster by using prope-legitimate seeds as plant materials. Seed germination selection phase can accelerate the obtainment of tolerant cocoa rootstocks. The tolerance trait of prope-legitimate seeds can be determined by seed germination on media induced by polyethylene glycol (PEG) solution. To determine the tolerant seeds, it is effective by using a specific character as selection criteria. This study aimed to obtain criteria and selection character to determine droughttolerant characteristic of prope-legitimate seed through PEG 6000 induction in the germination phase. The research was conducted at the Agronomy Laboratoryof the Indonesian Coffee and Cocoa Research Institute, Jember, East Java, Indonesia, used split plot design with five replications as experimental design. The main plot was solution of 0% and 6% PEG 6000, while the subplot were prope-legitimate seeds of 13 cocoa clones. The result showed that germination ability and sprout fresh weight were suitable as selection criteria to determine tolerant characteristic of prope-legitimate seeds to drought stress. Based on the value of  tress susceptible index and clustering analysis, the tested prope-legitimate seeds were divided into three groups. The group of tolerant genotype consisted of Sca 06, while the group of medium tolerant were ICCRI 03, Sulawesi 02, KW 641, TSH 858, KEE 02, Sulawesi 03, and KW 617. The susceptible group consisted of six genotypes, namely ICS 60, KKM 22, KW 516, Sulawesi 01, and MCC 02. 

ANALYSIS OF COMMERCIAL CAJUPUT OILS EXTRACTED FROM MELALEUCA CAJUPUTI POWELL BY GAS CHROMATOGRAPHY

2019 ◽  
pp. 7-10
Author(s):  
Thi Hoai Nguyen ◽  
Xuan Huyen Trang Hoang
Keyword(s):  
Gas Chromatography ◽  
Economic Development ◽  
Chemical Composition ◽  
Key Words ◽  
Melaleuca Cajuputi ◽  
Main Components ◽  
Main Component ◽  
Production Facilities

Background: Cajuput oil is a traditional and famous product of Thua Thien Hue, it is considered as a potential product, contributing to the economic development of Thua Thien Hue. Currently, there are many different products of cajuput oil in the market. However, the quality of cajuput oils in the production facilities has not been considered. The aim of the present study was to apply GC to the determination of the main components of commercial cajuput oils from M. cajuputi species in the Thua Thien Hue province. Materials and method: The chemical composition of 10 commercial cajuput oils extracted from leaves of Melaleuca cajuputi Powell collected in the Thua Thien Hue province was determined by gas chromatography. Results and conclusion: The major componentsofthe oils wereα-pinene, β-pinene, limonene, 1,8-cineoleand terpineol. The result obtained demonstrate that 1,8-cineole is the main component,present in 10 samples with values ranging from 15 to 59%. Key words: Cajuput oil, Melaleuca cajuputi, 1,8-cineole, α-pinene, β-pinene, limonene, terpineol

Tingkat Pemahaman Kompetensi Guru Pada Mahasiswa Pendidikan Geografi Sebagai Calon Guru Geografi

2020 ◽  
Vol 21 (1) ◽  
pp. 102-117
Author(s):  
Novia Zalmita ◽  
Muhajirah Muhajirah ◽  
Abdul Wahab Abdi
Keyword(s):  
Prospective Teachers ◽  
Prospective Teacher ◽  
Quantitative Description ◽  
Population Data ◽  
Geography Education ◽  
Teacher Competence ◽  
Education Department ◽  
Long Time

One that influences human resource indicators is education. The teacher is a profession as a job of academic specialization in a relatively long time in college. Understanding related to teacher competence is very important to have by a prospective teacher because it can affect the quality of performance as a professional teacher. The teacher's competence is known as pedagogic, professional, social and personality competencies. The issue in this study is how the competency of the teacher of the Department of Geography Education FKIP Unsyiah as a prospective teacher of geography? The purpose of this study was to determine the competence of teachers in the Department of Geography Education FKIP Unsyiah as prospective geography teachers. Quantitative description approach is used in this study to find answers to the issue. The population in this study were students of the Department of Geography Education FKIP Unsyiah class of 2015 and 2016 who had been declared to have passed the Micro Teaching and Magang Kependidikan 3 course totaling 50 people. Because the population is small and can be reached, the determination of the sample using total sampling techniques so that the sample in this study is the whole population. Data collection is done by distributing test questions to respondents. The data was analyzed using the descriptive statistics percentage formula. The results of the study indicate that the level of teacher competence of Geography Education Department students as prospective teachers is in the moderate category, namely as many as 22 respondents (44%). A total of 12 respondents (24%) were in the high category, 15 respondents (30%) were in the low category and 1 respondent (2%) were in the very low category.

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