scholarly journals Microbiological landscape of the periodontal pocket in inflammatory diseases in the periodontal tissues

2021 ◽  
Vol 25 (4) ◽  
pp. 332-338
Author(s):  
Stanislav V. Miklyaev ◽  
Olga M. Leonova ◽  
Andrey V. Sushchenko ◽  
Anton D. Kozlov ◽  
Ismail F. Agarizaev ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Inflammatory Diseases ◽  
Aggressive Periodontitis ◽  
Periodontal Pocket ◽  
Moderate Degree ◽  
Periodontal Tissues ◽  
Yeast Fungi ◽  
Microbiological Methods ◽  
Localized Aggressive Periodontitis ◽  
Group 2

Relevance. The study of the microbiological composition of periodontal pockets in inflammatory diseases of periodontal tissues is of great importance directly for the effective treatment and understanding of the pathogenetic stages of these diseases. Objective: to study the microbiological landscape of periodontal pockets with inflammatory diseases of periodontal tissues. Materials and Methods. 62 patients aged 20 to 45 years were selected and divided into groups depending on the form of periodontitis: with localized aggressive periodontitis - group №1, group №2 - with chronic generalized periodontitis, group № 3 - with exacerbation of chronic generalized periodontitis. The material for the study was taken from the periodontal pockets and examined by cultural and microbiological methods. Results and Discussion. It was found that the proportion and number of microbial associations in the periodontal pockets of different depths differs. In localized aggressive periodontitis, mainly facultative Gr+ (A. actinomycetemcomitans) and Gr- (St. aureus, Str. mitis) anaerobes were found in the periodontal pockets no more than 4 mm. In generalized chronic periodontitis of moderate degree, the same microorganisms were present in the periodontal pockets as in the localized form, but Str. mitis was more common among facultative aerobes. With the exacerbation of generalized chronic periodontitis from facultative anaerobes, St. aureus was more common and the proportion and number of A. actinomycetemcomitans significantly increased. Conclusion. According to the results of the study, the microorganisms that inhabit the periodontal pockets in inflammatory diseases of periodontal tissues mainly belong to facultative Gr+ and Gr - anaerobes, obligate Gr+ and Gr-anaerobes and yeast fungi.

scholarly journals Comparative evaluation of expression of VEGF (vascular endothelial growth factors) in periodontal tissues of healthy, chronic and aggressive periodontitis patients: an immunohistochemical study

2020 ◽  
Vol 9 (1) ◽  
pp. 784-788
Keyword(s):  
Growth Factors ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Vascular Endothelial ◽  
Healthy Controls ◽  
Periodontal Tissues ◽  
Inflammatory Status

Periodontitis is a chronic inflammatory disease of the vascularized supporting tissues of the teeth. Angiogenesis (neovascularization) is the budding of new capillaries and is thought to be an essential process in the development of chronic inflammatory diseases. Inflamed tissues (such as gingiva coincident in periodontal disease) have evidence of enhanced expression of inflammatory mediators, many of which can promote angiogenesis. Of the various cytokines and growth factors that are involved in angiogenesis, the most potent agent that acts specifically on vascular epithelium is Vascular Endothelial Growth Factor. Even though angiogenesis is a prominent feature of both inflammation and healing, information about its role in periodontal lesions is limited. Hence the aim of the present study was the immunohistochemical evaluation of the expression of VEGF in the gingival tissues of chronic and aggressive periodontitis patients compared to the healthy controls. The present study was carried out in a total of 45 subjects with age range of 18-55 years, reporting to the Department of Periodontology, Rajarajeswari Dental College and Hospital, Bangalore. Gingival tissue samples were collected from all the 45 subjects and categorized into three groups based on their clinical findings as follows: Group 1 (Healthy), Group II (Chronic Periodontitis), Group III (Aggressive Periodontitis). Following sample collection, immunohistochemical staining of tissues was carried out and evaluation was done to compare the grades of expression of VEGF in the three groups. The expression of VEGF in blood vessels was also quantitatively evaluated. The results were statistically analyzed using Kruskal Wallis ANOVA and Mann Whitney test. There was a statistically significant higher expression of VEGF in both chronic periodontitis and aggressive periodontitis group as compared to the control group. Aggressive periodontitis cases showed higher grades of expression of VEGF compared to the chronic periodontitis cases and healthy controls. However, the difference in expression of VEGF was not statistically significant between the two forms of periodontitis. The presence of VEGF in both chronic and aggressive periodontitis clearly indicates the potent role played by VEGF as an inflammatory agent in the initiation and progression of periodontal diseases. Thus, VEGF might be used as a potential vascular marker for the assessment of severity and inflammatory status in periodontal disease.

scholarly journals Condition of platelet factor of hemostasis system in pathogenesis of chronic periodontitis progression

2019 ◽  
Vol 27 (2) ◽  
pp. 209-218
Author(s):  
Evgeniya V. Kondyurova ◽  
Tat`yana I. Vlasova ◽  
Vladimir A. Trofimov ◽  
Aleksey P. Vlasov ◽  
Ruslan A. Adamchik ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Chronic Periodontitis ◽  
Cellular Component ◽  
Moderate Degree ◽  
Platelet Factor ◽  
Functional Changes ◽  
Periodontal Tissues ◽  
Hemostasis System ◽  
Platelet Membranes ◽  
Oxidative Processes

Аim. Тo evaluate the structural and functional condition of platelets as a cellular component of the hemostasis system in chronic generalized periodontitis of different severity, and to establish its role in the progression of the disease. Materials and Мethods. Clinical studies included 83 patients (47 men and 36 women) with chronic generalized periodontitis, comparable in age and sex, divided into three groups according to the severity of the disease: the first group (n=21) – periodontitis of mild severity; the second group (n=36) – moderate degree of severity; the third group (n=26) – severe periodontitis. Methods of the study included evaluation of the structural and functional characteristics of periodontal tissues, general hygienic condition of the oral cavity, the severity of the inflammatory process according to index parameters; study of microcirculation of periodontal tissues, determination of the evidence of oxidative processes in saliva, blood plasma, platelets, study of lipid composition of platelets. Results. In chronic generalized periodontitis, pathological changes in the lipid composition of platelet membranes (an increase in the percentage of lysophospholipids and free fatty acids) were associated with systemic activation of oxidative processes and of phospholipase enzyme systems, which was accompanied by an increase in their aggregation activity.The degree of increase in the activity of platelets was associated with the severity of microcirculatory disturbances in the periodontal tissues. The condition of the cellular component of the hemostasis system correlated with the severity of the pathological process in the periodontium. In severe degree of chronic periodontitis, structural and functional changes in platelets were maximal. Conclusion. In chronic periodontitis, the systemic activation of oxidative processes leads to a modification of the lipid composition of platelet membranes, which contributes to increase in their aggregation activity playing an important role in the induction of hypercoagulation, microcirculation disturbances and progression of inflammation in the periodontal tissues.

scholarly journals mRNA Expression of MMP-28 (Epilysin) in Gingival Tissues of Chronic and Aggressive Periodontitis Patients: A Reverse Transcriptase PCR Study

2013 ◽  
Vol 35 ◽  
pp. 113-118 ◽  
Author(s):  
P. Padmavati ◽  
S. Savita ◽  
B. M. Shivaprasad ◽  
Krishna Kripal ◽  
K. Rithesh
Keyword(s):  
Reverse Transcriptase ◽  
Mrna Expression ◽  
Chronic Periodontitis ◽  
Aggressive Periodontitis ◽  
Gingival Tissue ◽  
Tissue Samples ◽  
Normal Tissues ◽  
Periodontal Tissues ◽  
Probing Depth ◽  
The Difference

Background and Objectives. Matrix metalloproteinases degrade extracellular membrane and also release bioactive fragments and growth factors, thus influencing fundamental biological and pathological processes. Epilysin (MMP-28) differs from most other MMPs as it is expressed in a number of normal tissues, suggestive of functions in tissue homeostasis. The aim of the present study was to quantitatively evaluate and compare the mRNA expression of epilysin (MMP-28) in gingival tissues of healthy patients and of patients affected by chronic or aggressive periodontitis.Methods. A total of 60 subjects, 20 periodontally healthy subjects, 20 with chronic periodontitis, and 20 with aggressive periodontitis, were included in this study. Periodontal status was evaluated by measuring gingival index, probing depth and clinical attachment level. mRNA expression of MMP-28 was determined by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) in gingival tissue samples collected.Results. Relative quantification of mRNA expression of MMP-28 was highest in healthy tissues () when compared to subjects with chronic periodontitis () and aggressive periodontitis (), but the difference was not statistically significant.Conclusion. mRNA expression of MMP-28 was highest in healthy tissues when compared to diseased periodontal tissues suggesting that MMP-28 could act as a biomarker for periodontal health.

Effect on the inflammatory periodontal disease on quality of life of the individual and recovery possibilities

10.12737/4794 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-4
Author(s):  
Беленова ◽  
I. Belenova ◽  
Кобзева ◽  
Galina Kobzeva
Keyword(s):  
Quality Of Life ◽  
Chronic Periodontitis ◽  
Inflammatory Diseases ◽  
Dental Disease ◽  
Complex Treatment ◽  
Actual Problem ◽  
Periodontal Tissues ◽  
Spectrum Region ◽  
The Impact

Analysis of the current literature shows that treatment and prevention of inflammatory diseases of periodontal tissues remains an actual problem of dentistry in the XXI century. Existing approaches to the treatment, prevention and rehabilitation of patients with inflammatory diseases of parodont have certain disadvantages. The purpose of this study was to obtain positive results from the use of modulated diode light in the red spectrum region in the complex treatment of chronic periodontitis. The study shows the use of opportunities of determination of socio-dental indicators the social impact of dental disease in the clinical assessment of the status of periodontal tissues in the patients with chronic inflammatory diseases of gum. 59 patients were divided into 2 groups. The first group received traditional therapy and photodynamic therapy. The second group was a control. Assessment of the effect of dental disease on quality of life was carried out in all patients. The results of the study showed that after the complex treatment of chronic generalized periodontitis using modulated diode light in the red spectrum region, changes in the assessment of the impact of dental disease on quality of life are of stable positive. The results of the research motivate the necessity of applying and evaluating the impact of dental disease on quality of life in the rehabilitation of patients with chronic generalized parodontitis. The results of this study showed that after the complex treatment of chronic periodontitis using modulated diode light in the red spectrum region, changes in psychological status of patients have positive dynamics.

ANALYSIS OF THE CONTENTS OF THE PERIODONTAL POCKET IN CHRONIC PERIODONTITIS

2021 ◽  
pp. 51-55
Author(s):  
Станислав Валерьевич Микляев ◽  
Ольга Михайловна Леонова ◽  
Андрей Валерьевич Сущенко ◽  
Антон Дмитриевич Козлов
Keyword(s):  
Oral Cavity ◽  
Inflammatory Diseases ◽  
Periodontal Diseases ◽  
Pathological Process ◽  
Economic Problem ◽  
The Body ◽  
Periodontal Pocket ◽  
Periodontal Tissues ◽  
Normal Microflora ◽  
Obligate Anaerobic

Микрофлора человека - сложная саморегулирующая система, способная восстанавливаться при грамотной коррекции. Исследования последних лет показали, что нарушение нормального микробиоценоза полости рта растет из года в год и у жителей Российской Федерации превышает 90 %, вызывая при этом воспалительные заболевания в тканях пародонта, и, как правило, сопровождается дисбиозом полости рта, выраженность которого соответствует степени поражения пародонта. Одно из ведущих мест в развитии данной патологии занимает резидентная облигатно-анаэробная и микроаэрофильная микрофлора полости рта. Изучение микробиологического состава пародонтальных карманов (ПК) при воспалительных заболеваниях тканей пародонта (ВЗТП) имеет большое значение непосредственно для эффективного лечения и понимания патогенетических этапов этих заболеваний. Достаточно серьезной проблемой современной стоматологии как в нашей стране, так и за рубежом являются заболевания пародонта. Указанная патология снижает принятый ВОЗ критерий качества жизни и приводит к преждевременной потере зубов и представляет серьезную медицинскую, социальную и экономическую проблему. Первичным фактором, вызывающим поражение пародонта, являются бактерии зубного налета. Этиологическая структура инфекционных процессов в последнее десятилетие значительно изменилась, что связано с постоянной эволюцией микробов и вовлечением в патологический процесс условно-патогенных микробов, которые могут выступать в качестве комменсалов в составе нормальной микрофлоры и проявлять свою патогенность при снижении иммунного статуса организма The human microflora is a complex self-regulating system that can be restored with proper correction. Recent studies have shown that the violation of the normal microbiocenosis of the oral cavity increases from year to year and in the residents of the Russian Federation exceeds 90 %, causing inflammatory diseases in the periodontal tissues, and, as a rule, is accompanied by dysbiosis of the oral cavity, the severity of which corresponds to the degree of periodontal damage. One of the leading places in the development of this pathology is occupied by the resident obligate-anaerobic and microaerophilic microflora of the oral cavity. The study of the microbiological composition of periodontal pockets (PC) in inflammatory diseases of periodontal tissues (VSTP) is of great importance directly for the effective treatment and understanding of the pathogenetic stages of these diseases. Quite a serious problem of modern dentistry both in our country and abroad are periodontal diseases. This pathology reduces the WHO-accepted quality of life criterion and leads to premature tooth loss, and is a serious medical, social and economic problem. The primary factor that causes periodontal disease is plaque bacteria. The etiological structure of infectious processes has changed significantly in the last decade, which is due to the constant evolution of microbes and the involvement of conditionally pathogenic microbes in the pathological process, which can act as commensals in the normal microflora and show their pathogenicity with a decrease in the immune status of the body

scholarly journals A COMPARATIVE EXAMINATION OF QUANTITATIVE AND SPECIFIC STRUCTURE OF YEAST FUNGI OF THE CANDIDA GENUS CONSIDERING PATIENTS WITH CHRONIC GENERALIZED PERIODONTITIS OF VARYING SEVERITY

2017 ◽  
Vol 72 (2) ◽  
pp. 143-148
Author(s):  
G. V. Volchenkova ◽  
S. V. Kiryushenkova ◽  
A. I. Nikolaev ◽  
V. R. Shashmurina ◽  
L. I. Devlikanova
Keyword(s):  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Age Groups ◽  
Tissue Response ◽  
Periodontal Pocket ◽  
Control Group ◽  
Quantitative Composition ◽  
Group 4 ◽  
Yeast Fungi ◽  
Comparative Examination

Background: Periodontitis is a major reason of tooth loss in people of middle and older age groups that requires more attention of researchers to the problems of etiology, pathogenesis, diagnosis, and therapy of the disease. Currently, the interaction of the microbial content of dental plaque and local tissue response is considered to be the main reason for the development of inflammatory periodontal diseases. At the same time, Russian and foreign researchers mark a high frequency of so-called torpid or resistant to antimicrobial therapy forms of periodontitis.Aim: To study the specific and quantitative composition of a fungi of the Candida genus in the contents of periodontal pockets of patients with varying severity of chronic generalized periodontitis.Materials and methods: A non-randomized non-controlled retrospective research was conducted. We studied the main clinical and radiological indicators of periodontal status and its contamination with fungi of the Candida genus in people with mild, moderate, or severe chronic periodontitis.Results: Research participants enrolled in four groups: 1–3 groups included patients with chronic generalized light, medium and heavy periodontitis; group 4 included twenty-five subjects with no signs of periodontal disease. Among the examined patients from the control group, the frequency allocation of Candida from the contents of the gingival sulcus was 9.09%, patients with periodontal pathology of periodontal pockets ― 64.91%. High contamination with fungal flora (more than 6.0 CFU / mL) was observed in all patients with chronic periodontitis. Total allocated 40 strains of Candida with a predominance of Candida albicans.Conclusions: The results of microbiological research confirmed an increase of contamination of periodontal with fungi of the Candida genus in patients with periodontitis if compared with healthy people. The degree of the contamination with fungi of the Candida genus decreased with increasing severity of periodontitis. It can be assumed that the presence of Candida fungi in the periodontal pocket can be considered as a possible component of the etiology of chronic periodontitis and as a factor aggravating it.

scholarly journals Effect of Non-Surgical Periodontal Therapy on Interleukin-29 Levels in Gingival Crevicular Fluid of Chronic Periodontitis and Aggressive Periodontitis Patients

2013 ◽  
Vol 34 (1) ◽  
pp. 1-7 ◽  
Author(s):  
B. M. Shivaprasad ◽  
A. R. Pradeep
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Aggressive Periodontitis ◽  
Periodontal Therapy ◽  
Scaling And Root Planing ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Crevicular Fluid ◽  
Group 1

Recently discovered interleukin 29 (IL-29) has antiviral properties and its production is induced by herpes viruses. This study was aimed at analyzing the effect of non-surgical periodontal treatment on IL-29 levels in gingival crevicular fluid (GCF) of chronic and aggressive periodontitis patients. A total of 60 participants were divided into healthy group (group 1;n= 20), chronic periodontitis group (group 2;n= 20), and aggressive periodontitis group (group 3;n= 20). GCF samples collected from each subject at baseline and 6–8 weeks after scaling and root planing were quantified for IL-29 levels using ELISA. The mean IL-29 concentration in GCF was found to be highest in group 3 (92.37 pg/μl). The mean IL-29 level in group 1 and group 2 was 36.88 pg/μl and 69.35 pg/μl respectively. After scaling and root planing, the mean concentration of IL-29 in GCF was increased to 85.99 pg/μl in group 2 and to 114.64 pg/μl in group 3. Results of the present study indicate that antiviral IL-29 level was highest in GCF of aggressive periodontitis patients and least in subjects with healthy periodontium, while that of chronic periodontitis lying in between. After non-surgical periodontal therapy, IL-29 levels increased both in chronic and aggressive periodontitis patients and deserve further investigation as a potential therapeutic agent in treating periodontitis.

Comparative analysis of polymorphic variants of IL-17A and MMP-1 genes with the risk of developing chronic periodontitis in petrochemical workers

2020 ◽  
Vol 60 (10) ◽  
pp. 687-693
Author(s):  
Iskander I. Zaidullin ◽  
Denis O. Karimov ◽  
Lilija K. Karimova ◽  
Milyausha F. Kabirova ◽  
Rasima R. Galimova ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Clinical Manifestations ◽  
Control Group ◽  
Periodontal Tissues ◽  
Dental Examination ◽  
Number Of Patients ◽  
Increased Risk ◽  
Harmful Substances

The susceptibility to the development and progression of inflammatory periodontal diseases, which depends on genetic and external factors (smoking, stress, oral hygiene), varies widely. In the development of these diseases, an important role is played not only by the presence of periodontal pathogenic microorganisms, but also by the presence of congenital or acquired immunodeficiency, immunoregulatory defects. The immune system plays a key role in the physiological and pathological processes of periodontal tissues. In this regard, IL17, produced by CD4+ Th cells, which has both Pro-inflammatory and protective activity, is of particular interest in the pathogenesis of periodontitis. The aim of study was to identify the relationship between polymorphic loci of the IL-17A (rs2275913) and MMP-1 (rs1799750) genes and clinical manifestations of chronic periodontitis in petrochemical workers. Dental examination was performed in 92 ethylene oxide production workers with chronic periodontitis and 74 patients with chronic periodontitis who did not come into contact with chemical factors (control group). Genotyping of polymorphisms rs2275913 of the IL17A gene and rs1799750 of the MMP1 gene was performed by allele-specific real-time polymerase chain reaction (PCR). Hygienic assessment of the degree of air pollution of the working area with harmful substances was carried out by gas chromatography according to the guidelines for the determination of harmful substances in the air № 5098-89, № 3119-84. When comparing the results of studies of both groups, there were no statistically significant differences in the frequency distributions of allelic variants and genotypes of the IL-17A and MMP-1 genes. The AA/AG genotypes of the IL-17A gene were associated with an increased risk of severe disease compared to the GG genotype in workers in the main group (OR=6.1; 95% CI 1.33-28.5; p=0.021) and in the control group (OR=7.26; 95% CI 1.34-39.25; p=0.016). Carriers of the A allele in the control group increased the risk of severe chronic periodontitis by 2.4 times compared to carriers of the G allele (OR=2.41; 95% CI 1.19-4.87; p=0.014). During the dental examination of employees of the ethylene oxide plant, the clinical course of periodontal diseases was more severe in comparison with the control group, and the number of patients with severe periodontitis was twice as high. It was found that the AA/AG genotypes of the IL-17A gene and the carrier of the A allele are associated with increased susceptibility to the development of severe chronic periodontitis. The association between the MMP-1 gene polymorphism and the risk of severe forms of chronic periodontitis has not been established. A risk factor for the development of inflammatory periodontal diseases in employees of the petrochemical complex is a complex of harmful production factors.

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