scholarly journals Physical and oxidative stability of n-3 delivery emulsions added seaweed-based polysaccharide extracts from Nordic brown algae Saccharina latissima

2021 ◽  
Author(s):  
Ditte Hermund ◽  
Ioanna Anagnostara ◽  
Xiaoru Hou ◽  
Maria Mikkelsen ◽  
Nanna Rhein-Knudsen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Stability ◽  
Sodium Alginate ◽  
Brown Algae ◽  
Physical Stability ◽  
Droplet Size Distribution ◽  
Saccharina Latissima ◽  
Mixed Composition ◽  
G Ratio

Enriching foods with long-chain (LC) n-3 polyunsaturated fatty acids (PUFAs) requires a delivery emulsion system, which is both thermodynamically and oxidatively stable. The antioxidant and stabilizing properties of three types of polysaccharide extracts from brown algae Saccharina latissima with mixed composition of polysaccharides (SA: 98% Sodium alginate, SF: 90% alginate and 9% fucoidan, SL: 14.5% fucoidan, 9.5% laminarin and co-extracted non-polysaccharides) were evaluated. SA, SF, SL and REF (added commercial sodium alginate) all showed in vitro ferrous ion chelating ability in the order: SA(99%)>SL(78%)>REF(31%)>SF(16%). The difference in antioxidant activity between SA, REF and SF appeared related to structural differences of alginate (M/G ratio). A storage trial was conducted using 70% (w/w) fish oil-in-water delivery emulsions added sodium caseinate (NaCas) (0.23 wt%) as emulsifier in combination with SA, SF, SL or commercial NaAlg (REF) in different concentrations (C1=0.1, C2=0.2 C3=0.3 and C4=0.4 wt%). A control with only NaCas were included (CON). The physical (e.g. creaming and droplet-size distribution) and oxidative (peroxide value and volatiles) stability of the emulsions, were evaluated (12 days, dark at 20˚C). Acceptable physical stability (creaming index, CI) was found for, REF (all concentrations), SF=0.2 wt% (C2), SL and SA=0.3 wt% (C3) and 0.4 wt% (C4). In general, the oxidative stability decreased by adding REF, SA and SF (except for REF at C1), as prooxidant activity was observed. However, SA showed antioxidant activity against formation of 2-ethylfuran. SL showed antioxidant activity in decreasing formation of volatile compounds in emulsions when added in concentrations above 0.2 wt%.

scholarly journals Antioxidant and antimitotic activities of sulfated polysaccharide from marine brown algae Padina tetrastromatica

2015 ◽  
Vol 7 ◽  
Author(s):  
Geena Mariya Jose ◽  
Anitha Radhakrishnan ◽  
G Muraleedhara Kurup
Keyword(s):  
Antioxidant Activity ◽  
Brown Algae ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sulfated Polysaccharide ◽  
Sulfated Polysaccharides ◽  
Scavenging Activity ◽  
Padina Tetrastromatica ◽  
Nitric Oxide Radical

<p class="Default">Antioxidants play a central role in the prevention of carcinogenesis. The most natural compounds exhibit their protective effects by eliciting antioxidant potential. Sulfated polysaccharide was isolated from the brown algae <em>Padina tetrastromatica</em>, then purified and evaluated for its composition and <em>in vitro </em>antioxidant and antimitotic activities. Both ethanolic sulfated polysaccharide (ESPS) and ethanolic sulfated polysaccharide-column purified (ESPS-CP) exhibited considerable amount of carbohydrates (11.2% and 17.6%), sulfate (11.4% and 7.4%), fucose (5.5% and 15.7%), uronic acid (4.7% and 11.8%), xylose (0.5% and 0.03%) and sulfated polysaccharide (2.4% and 12.7%) content. The FTIR analysis and phytochemical screening also confirmed the presence of sulfated polysaccharides. In the <em>in vitro</em> antioxidant activity determination using DPPH (1-1-diphenyl 2-picryl hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity and reducing power, ESPS showed more activity than ESPS-CP. In the case of nitric oxide radical scavenging, ESPS-CP was found to be more effective. At a concentration of 2mg/ml, both samples were potent antioxidants with significant IC<sub>50</sub> values. The antimitotic studies such as mitotic index in onion root tips and sprouting assay in green gram seeds also proved that both the extracts are able to prevent mitosis. The extrapolation of these results can find opportunities in therapeutic regiments of cancer.<strong></strong></p>

scholarly journals β-Sitosterol Loaded Nanostructured Lipid Carrier: Physical and Oxidative Stability, In Vitro Simulated Digestion and Hypocholesterolemic Activity

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 386
Author(s):  
Yasamin Soleimanian ◽  
Sayed Amir Hossein Goli ◽  
Jaleh Varshosaz ◽  
Lorenzo Di Cesare Mannelli ◽  
Carla Ghelardini ◽  
...  
Keyword(s):  
Oxidative Stability ◽  
Oral Delivery ◽  
Physical Stability ◽  
Density Lipoprotein ◽  
Water Soluble ◽  
Nanostructured Lipid Carrier ◽  
Lipid Mixture ◽  
Simulated Digestion

The objective of the present study was to explore the potential of nanostructured lipid carriers (NLCs) for improving the oral delivery of β-sitosterol, a poorly water-soluble bioactive component with hypocholesterolemic activity. Two β-sitosterol formulations with different solid lipid compositions were prepared by melt emulsification, followed by the sonication technique, and the effect of storage conditions and simulated digestion on the physical, chemical and oxidative stability, bioaccessibility and release were extensively studied. Both NLC preparations remained relatively stable during the four weeks of storage at different conditions (4, 25 and 40 °C), with more superior stability at lower temperatures. The in vitro digestion experiment indicated a high physical stability after exposure to the simulated mouth and stomach stages and an improved overall β-sitosterol bioaccessibility at the end of the digestion. The NLCs presented an increased solubility and gradual release which could be justified by the remarkable affinity of β-sitosterol to the complex lipid mixture. An in vivo study demonstrated an improved reduction in the total cholesterol and low-density lipoprotein cholesterol plasma levels in mice compared with the drug suspension. These investigations evidenced the potential of the developed NLC formulations for the enhancement of solubility and in vivo performance of β-sitosterol.

scholarly journals Uji Stabilitas Dan Aktivitas Antioksidan Mikroemulsi Likopen Tomat (Solanum lycopersicum L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 10-17
Author(s):  
Evi Sulastri ◽  
Mohamad Ikram ◽  
Yuliet Yuliet
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Tween 80 ◽  
Antioxidant Power ◽  
Solubility In Water ◽  
Solanum Lycopersicum L ◽  
Globule Size ◽  
Ic50 Value ◽  
Physical And Chemical

Lycopene is a class of keratonoid that have been proven has antioxidant activity but has limited solubility in water. So as to boost their solubility, in this study conducted in the form of microemulsion formulations. This study aims to get a tomato lycopene extract microemulsion which is stable and has antioxidant effects. The research was initiated to test the antioxidant power of tomato lycopene extract in vitro against DPPH. Tomato lycopene extract microemulsion is formulated based on the optimization of the composition of the base consisting of the VCO as the oil phase, tween 80 as surfactant and glycerin as cosurfactant. Preparations produced were tested for antioxidant activity and their physical and chemical stability testing which include: organoleptic, viscosity, globule size and pH during 28 days of storage. The results showed that tomato lycopene extract formula microemulsion stable composition of tomato lycopene extract 0.3%, VCO 15%, tween 80% and 35% glycerin. The test results obtained physical stability of the microemulsion formula elected tomato lycopene extract has a distinctive scent of aromatic, orange, thick, globule size of <5 μm, viscosity (600, 33 ± 69.29 to 746 cPs ± 8.32), and pH ( 6.27 ± 0.20 to 6.79 ± 0.10). As very strong antioxidant activity with IC50 value of 29.07 ppm.

Isolation of Fucoxanthin from Brown Algae and Its Antioxidant Activity: In Vitro and 5% Fish Oil-In-Water Emulsion

2018 ◽  
Vol 95 (7) ◽  
pp. 835-843 ◽  
Author(s):  
Sabeena Farvin Koduvayur Habeebullah ◽  
Alagarsamy Surendraraj ◽  
Charlotte Jacobsen
Keyword(s):  
Antioxidant Activity ◽  
Fish Oil ◽  
Brown Algae ◽  
Water Emulsion ◽  
Oil In Water ◽  
Oil In Water Emulsion

scholarly journals Uji Aktivitas Antioksidan Minyak Argan (Argania spinosa L.) Dalam Bentuk Sediaan Mikroemulsi

2021 ◽  
Vol 1 (1) ◽  
pp. 30-39
Author(s):  
Nur Ain Thomas ◽  
Adiva Sidangoli
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
P Value ◽  
Freeze Thaw ◽  
Argania Spinosa ◽  
Globule Size ◽  
Ic50 Value ◽  
Dpph Method ◽  
Oil Water

Argan oil (Argania spinosa L.) is an oil that has benefits as a natural antioxidant which is good for health. Microemulsion is a thermodynamically stable delivery system;  transparent;  has a small globule size and consists of a mixture of oil, water, surfactant and co-surfactant which has great potential in delivering dermal active substances by increasing transdermal permeability in topical drug delivery. This research aimed to formulate argan (Argania spinosa L.) oil into microemulsion dosage form and determine the antioxidant activity using DPPH method. The study began with the optimization of base into several concentration variations of surfactant and co-surfactant. The formula made into 7 namely F1, F2, F3, F4, F5, F6 and F7, evaluated using organoleptic test and centrifugation test. The results showed the base of F6 which had the clear, transparent physical appearance and no separation occured. The F6 base was then made into microemulsion dosage with 3 concentration variations of argan (Argania spinosa L.) oil, namely F1 (0.5%), F2 (1%) and F3 (1.5%). The 3 formulas were tested for the physical stability which included pH test, viscosity test, centrifugation test and freeze-thaw test.  The testing of antioxidant activity carried out by in vitro using the DPPH method which then calculated for the IC50 value on t0 and t28. From the results of the physical stability test at room temperature and freeze-thaw, the 3 formulas met the organoleptic, pH and centrifugation tests. The antioxidant activity values of IC50 were F1 (t0 = 291.14 g/mL; t28 = 230.43 g/mL), F2 (t0 = 89.02 g/mL; t28 = 129.01 g/mL), F3 (t0 = 81.07 g/mL; t28 = 116.30 g/mL). The statistical test result of T test showed the p value = 0,896, (0.05), which indicated that there was no significance difference in the antioxidant activity result between the 3 formulas on t0and t28.

scholarly journals Physical and oxidative stability of n‐3 delivery emulsions added seaweed‐based polysaccharide extracts from Nordic brown algae Saccharina latissima

2021 ◽  
Author(s):  
Ditte B. Hermund ◽  
Ioanna Anagnostara ◽  
Xiaoru Hou ◽  
Maria Dalgaard Mikkelsen ◽  
Nanna Rhein‐Knudsen ◽  
...  
Keyword(s):  
Oxidative Stability ◽  
Brown Algae ◽  
Saccharina Latissima

scholarly journals Impact of Pleurotus ostreatus β-Glucans on Oxidative Stability of Active Compounds Encapsulated in Powders during Storage and In Vitro Digestion

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1219
Author(s):  
Francesca Gallotti ◽  
Anaïs Lavoisier ◽  
Christelle Turchiuli ◽  
Vera Lavelli
Keyword(s):  
Oxidative Stability ◽  
Size Distribution ◽  
Pleurotus Ostreatus ◽  
Accelerated Aging ◽  
Droplet Size Distribution ◽  
In Vitro Digestion ◽  
Bimodal Distribution ◽  
Wall Material ◽  
Acacia Gum

Polyunsaturated fatty acids and α-tocopherol were encapsulated in powders by spray drying using maltodextrins DE 12 as wall material and different emulsifiers (Tween®20, acacia gum or β-glucans-rich extracts from Pleurotus ostreatus). The aim was to study the effects of the surfactants on: (a) the oil droplet size distribution and α-tocopherol stability during in vitro digestion, and (b) the oxidative stability during 15 days of accelerated storage. Acacia gum sample had the most stable particle size distribution up to the gastric phase, but showed a significant α-tocopherol degradation prior to the intestinal stage. On the contrary, β-glucan-samples displayed a bimodal distribution in the oral and gastric phases but retained α-tocopherol up to the beginning of the intestinal stage. At the end of intestinal stage, no α-tocopherol was found in the samples. The storage study showed that β-glucans improved the oxidative stability of the powders, which displayed 82% α-tocopherol retention after 5 days under accelerated conditions (60 °C), corresponding to 310 days at 20 °C, while acacia gum and Tween® 20 did not delay α-tocopherol degradation. Results highlight the potential antioxidant activity of β-glucans used as emulsifying agents during in vitro digestion and accelerated aging conditions.

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