scholarly journals Antioxidant and antimicrobial activities of Penicillium sp. lectins

2019 ◽  
Vol 71 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Ram Singh ◽  
Amandeep Walia
Keyword(s):  
Biomedical Applications ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Peroxide Radical ◽  
Gram Positive Bacteria ◽  
Penicillium Sp ◽  
Biological Potential ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Antioxidant And Antimicrobial Activities

Lectins are a diverse group of proteins of non-immune origin that interact specifically with glycans. Owing to their specificity, they can mediate various cellular and molecular recognition processes. To explore information on biological activities of lectins from Penicillium duclauxii, P. proteolyticum and P. griseoroseum, they were investigated for their antioxidant and antimicrobial activities. Penicillium sp. lectins exhibited moderate antioxidant activity. P. duclauxii, P. proteolyticum and P. griseoroseum lectins inhibited DPPH with an IC50 value of 71.42, 75.04 and 82.11 ?g/mL, respectively. P. duclauxii, P. proteolyticum and P. griseoroseum lectins inhibited the hydrogen peroxide radical with IC50 values of 198.57, 209.76 and 215.31 ?g/mL, respectively. P. duclauxii and P. proteolyticum lectins exhibited potent antibacterial activity against Gram-negative bacteria. P. griseoroseum lectin inhibited only Gram-positive bacteria. Penicillium sp. lectins did not exhibit antifungal activity. The biological potential of Penicillium sp. lectins will help to understand their biomedical applications. This is the first report on the antioxidant and antimicrobial activities of purified lectins from Penicillium sp.

scholarly journals Anthelminthic, Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and FTIR Analyses of Vernonia camporum Stem-Bark

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Clement Osei Akoto ◽  
Akwasi Acheampong ◽  
Yaw Duah Boakye ◽  
Bernard Asante ◽  
Samuel Ohene ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Dilution Method ◽  
Ic50 Values ◽  
Hexane Extracts ◽  
Anti Inflammatory ◽  
Antioxidant And Antimicrobial Activities

Vernonia camporum is used ethnomedicinally to treat diseases such as malaria, fever, hypertension, mouth sores, pains, inflammation, and skin rashes in Africa. This study aims at investigating the biological activities (anthelminthic, anti-inflammatory, antioxidant, and antimicrobial) and separating some of the biologically active components in the methanol crude extract of the stem-bark of Vernonia camporum. Phytochemical screening, anthelminthic (Lumbricus terrestris (earthworm)), anti-inflammatory (egg albumin denaturation), antioxidant (DPPH and H2O2 scavenging and total antioxidant capacity (TAC)), and antimicrobial (agar and broth dilution method) assays were performed on the extracts. Column chromatography and FTIR spectroscopic analysis were employed to separate and analyze the least polar constituents of the methanol extract. The preliminary phytochemical analyses revealed the presence of secondary metabolites such as alkaloids, phenols, tannins, saponins, terpenoids, flavonoids, steroids, phytosterols, proteins and amino acids, phlobatannins, glycosides, cardiac glycosides, coumarins, and anthocyanins. The extracts (methanol and hexane) showed a higher concentration-dependent anthelminthic activity. The extracts exhibited high concentration-dependent anti-inflammatory activities with IC50 values of 35.83 ± 3.984 and 53.91 ± 5.8413 µg/mL, respectively. The IC50 values for methanol and hexane extracts in the DPPH assay were 17.70 ± 3.430 and 29.54 ± 1.5437 µg/mL, and those of the H2O2 assay were 243.20 ± 0.1528 and 352.20 ± 12.64 µg/mL, respectively. TAC results for methanol and hexane extracts were recorded as 31.592 ± 1.682 and 30.232 ± 0.445 gAAE/100 g, respectively. The extracts were observed to exhibit antimicrobial activity against test organisms with MICs ranging from 0.1953 to 25.00 mg/mL. Chromatographic separations gave four fractions. FTIR analysis indicated the presence of various functional groups in purified fractions of the methanol extract that confirms the presence of the phytochemicals identified in the screening test. The results indicate that both extracts of V. camporum possess anthelminthic, anti-inflammatory, antioxidant, and antimicrobial activities supporting the traditional usage of this plant.

scholarly journals Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Kamel Msaada ◽  
Nidhal Salem ◽  
Olfa Bachrouch ◽  
Slim Bousselmi ◽  
Sonia Tammar ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

Antioxidant and antimicrobial activities of stem bark extracts of Oroxylum indicum Vent. (Bignoniaceae) ‐ A medicinal plant of northeastern India

2011 ◽  
Vol 1 (3) ◽  
pp. 152-157
Author(s):  
Vimal Kumar ◽  
A. K. Chaurasia ◽  
Ashok Naglot ◽  
Reji Gopalakrishnan ◽  
B. J. Gogoi ◽  
...  
Keyword(s):  
Ethyl Acetate Extract ◽  
Water Extract ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Chloroform Extract ◽  
Antioxidant Ability ◽  
Oroxylum Indicum ◽  
Ic50 Value ◽  
Trolox Equivalent ◽  
Antioxidant And Antimicrobial Activities

The antioxidant activity of extracts of Oroxylum indicum stem bark wasevaluated using different methods. The ethyl acetate extract (EAE) showedthe lowest IC50 value (0.76 mg/ml) with the highest inhibition of DPPH. In theFRAP assay, the hexane extract (HE) showed the highest ferric reducing abilitywhereas the EAE showed better antioxidant ability with lowest IC50 value(0.80mg/ml) in βCL assay. The EAE and HE showed highest ascorbic acidequivalent (6.60 ± 0.22) and trolox equivalent (2.04 ± 0.07) values in antioxidativecapacity of ACW and ACL in PCL assay. During antimicrobial evaluationHE, Chloroform extract (CE) and EAE were found effective against thetested bacteria while the water extract (WE) showed better activity (14.25 ±0.478) against yeast.

Composition, Antioxidant and Antimicrobial Activities of the Leaf Essential Oil of Machilus Japonica from Taiwan

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700
Author(s):  
Chen-Lung Ho ◽  
Yu-Chang Su
Keyword(s):  
Essential Oil ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Disc Diffusion ◽  
Gram Positive Bacteria ◽  
Leaf Essential Oil ◽  
Inhibition Zones ◽  
Main Components ◽  
Broth Dilution ◽  
Antioxidant And Antimicrobial Activities

The chemical composition, and antioxidant and antimicrobial activities of the essential oil isolated from the leaf of Machilus japonica from Taiwan have been investigated. The essential oil from the fresh leaves was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 97 compounds were identified, representing 100% of the oil. The main components identified were α-phellandrene (14.5%), α-pinene (12.8%), thymol (12.6%), β-pinene (8.3%), α-terpineol (6.5%) and carvacrol (6.0%). The antioxidant activity of the oil was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capability test. The results showed that the IC50 was 51.8 μg/mL. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast, with inhibition zones of 48~54 mm and MIC values of 16.12~32.25 μg/mL, respectively. For the antioxidant and antimicrobial activities of the oil, the active source compounds were determined to be thymol and carvacrol.

scholarly journals Sesquiterpenes-Rich Essential Oil from Above Ground Parts of Pulicaria somalensis Exhibited Antioxidant Activity and Allelopathic Effect on Weeds

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 399 ◽  
Author(s):  
Abdulaziz Assaeed ◽  
Abdelsamed Elshamy ◽  
Abd El-Nasser El Gendy ◽  
Basharat Dar ◽  
Saud Al-Rowaily ◽  
...  
Keyword(s):  
Essential Oil ◽  
Biological Activities ◽  
Allelopathic Effect ◽  
Bidens Pilosa ◽  
Specific Chemical ◽  
Genetic Characteristics ◽  
Dactyloctenium Aegyptium ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Main Components

Pulicaria genus (fleabane) is characterized by its fragrant odor due to the presence of essential oil (EO). According to the literature reviews, the EO of Pulicaria somalensis O.Hoffm. (Shie) is still unexplored. For the first time, 71 compounds were characterized in EO derived from above-ground parts of P. somalensis collected from Saudi Arabia. Sesquiterpenes represented the main components (91.8%), along with minor amounts of mono-, diterpenes, and hydrocarbons. Juniper camphor (24.7%), α-sinensal (7.7%), 6-epi-shyobunol (6.6%), α-zingiberene (5.8%), α-bisabolol (5.3%), and T-muurolol (4.7%) were characterized as main constituents. The correlation analysis between different Pulicaria species showed that P. somalensis has a specific chemical pattern of the EO, thereby no correlation was observed with other reported Pulicaria species. The EO showed significant allelopathic activity against the weeds of Dactyloctenium aegyptium (L.) Willd. (crowfoot grass) and Bidens pilosa L. (hairy beggarticks). The IC50 value on the germination of D. aegyptium was double that of B. pilosa. The IC50 values on the root growth of B. pilosa and D. aegyptium were 0.6 mg mL−1 each, while the shoot growths were 1.0 and 0.7 mg mL−1, respectively. This variation in the activity could be attributed to the genetic characteristics of the weeds. Moreover, the EO exhibited significant antioxidant effects compared to ascorbic acid. Further studies are necessary to verify if these biological activities of the EO could be attributable to its major compounds.

scholarly journals Phytochemical characterization, and antioxidant and antimicrobial activities of white cabbage extract on the quality and shelf life of raw beef during refrigerated storage

RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41430-41442
Author(s):  
Momna Rubab ◽  
Ramachandran Chelliah ◽  
Kandasamy Saravanakumar ◽  
Jong-Rae Kim ◽  
Daesang Yoo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Antimicrobial Activities ◽  
Refrigerated Storage ◽  
White Cabbage ◽  
Brassica Vegetables ◽  
Cabbage Extract ◽  
Antioxidant And Antimicrobial Activities ◽  
Quality And Shelf Life

Brassica vegetables are well-characterized, containing a wide-spectrum of phenolic compounds that are responsible for their diverse biological activities like antioxidant and antimicrobial activities.

scholarly journals PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIMICROBIAL ASSESSMENT OF PLUCHEA INDICA (L.) LESS EXTRACT AS AN ACTIVE INGREDIENT IN NATURAL LOTION BAR

2021 ◽  
pp. 51-57
Author(s):  
SIRIKHWAN TINRAT
Keyword(s):  
Broad Spectrum ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Reducing Ability ◽  
Phytochemical Compounds ◽  
Antioxidant And Antimicrobial Activities

Objective: To screen phytochemical compounds and study antioxidant and antimicrobial activities of Pluchea indica (L.) Less (PI) leaves extracts for assessment the moisturizing lotion bar formulation containing it’s as a bioactive ingredient. Methods: PI leaves were extracted in different solvents. Crude extracts were screened phytochemical compounds and evaluated antioxidant and antimicrobial activities before formulating to antibacterial moisturizing lotion bar. Results: 95% ethanolic extract displayed high antioxidant activities. DPPH radical scavenging capacity with IC50 was 6.80±0.04 mg/ml (61.37±0.41%) and the ferric reducing ability power was 381.20±0.10 mg AAE/100 gDW. Additionally, 95% ethanolic extract served as a broad spectrum bacteriostatic agents against gram-positive and gram-negative species with an inhibition zone of 6.50±0.00-46.67±0.58 mm. It also was simultaneously as a broad spectrum bactericidal agents (MIC and MBC value of 25-200 mg/ml) by broth micro-dilution method. The screened phytochemical constituents (saponins, tannins, flavonoids, terpenoids, steroids, and alkaloids) in PI leaves extract may be responsible for high biological activities. Thus, 95% ethanolic extract was the most appropriate bioactive ingredient for formulated the product development. Conclusion: The developed moisturizing lotion bar with 0.0041% of 95% ethanolic extract was the most eligible formula that showed good appearance, odor, color, homogeneity, after feel and stability. This extract-containing lotion bar formulation achieved a high level of customer satisfaction by sensory evaluation and development of this specific formulation should be subject to further clinical investigation.

scholarly journals Synthesis and Antimicrobial Activities of N-(Heteroaryl-substituted)-p-toluenesulphonamides

2014 ◽  
Vol 2014 ◽  
pp. 1-5
Author(s):  
Ozoh Chinwe Francisca ◽  
Okoro Uchechukwu Chris ◽  
Ugwu David Izuchukwu
Keyword(s):  
Biological Activities ◽  
Condensation Reaction ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Gram Positive Bacteria ◽  
New Class ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal ◽  
C Nmr

A new class of N-(heteroaryl-substituted)-p-toluenesulphonamides has been synthesized exhibiting antibacterial and antifungal properties. The condensation reaction of p-toluenesulphonyl chloride 1 with appropriate substituted amino pyridines 2a–g in acetone furnished N-(heteroaryl-substituted)-p-toluenesulphonamides 3a–g. These derivatives were characterized by IR, 1H-, and 13C-NMR spectroscopy and were screened in vitro against gram-positive bacteria, gram-negative bacteria, and fungi organisms using agar-diffusion method. Results indicated improved biological activities over reference drugs such as Tetracycline (TCN) and Fluconazole (FLU).

scholarly journals Phenolic profile and biological potential of wild blackberry (Rubus discolor) fruits

2021 ◽  
Vol 45 (2) ◽  
pp. 215-222
Author(s):  
Ivona Velickovic ◽  
Zeljko Zizak ◽  
Natasa Simin ◽  
Kristina Bekvalac ◽  
Marija Ivanov ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Penicillium Verrucosum ◽  
Biological Potential ◽  
Ic50 Values ◽  
Ethanol Extracts

The berries of Rubus discolor are considered a rich source of phytochemicals which could play an important role in the prevention of prevalent contemporary chronic diseases. Thus, the goal of the presented study was to determine the profile of phenolic acids and anthocyanins of aqueous and ethanol extracts of R. discolor fruit, and their free radical scavenging, antiproliferative, antidiabetic and antimicrobial activities. LC-MS/MS analyses confirmed the presence of 11 phenolic acids with protocatechuic and gallic acids being the major compounds. Additionally, cyanidin- glucoside/galactoside was the most abundant among the five identified anthocyanins. The ethanol extract was more efficient in scavenging free radicals than the aqueous extract. The absence of antiproliferative activity was observed for both extracts. However, they inhibited carbohydrate hydrolysing digestive enzymes associated with type-2 diabetes. Furthermore, the obtained results for ?-glucosidase inhibitory activity (IC50 values 44.52 and 80.72 ?g/mL, for the aqueous and ethanol extracts, respectively) indicate significantly higher activity than the positive control, GlucobayR (233.38 ?g/mL). The ethanol extract was more effective against all of the examined bacteria (Bacillus cereus, Staphylococcus aureus and Enterobacter cloacae) than the aqueous extract. On the contrary, the aqueous extract showed better antifungal properties, particularly against Trihoderma viride and Penicillium verrucosum var. cyclopium. The quantified phenolics and presented bioactivities of R. discolor fruit extracts candidate them as a potential source of bioactive compounds which might be used in the food, pharmacy and cosmetic industries.

scholarly journals Cytotoxic Activity-Guided Isolation from Achillea monocephala, and Biological Activities of its Different Extracts

2020 ◽  
Vol 8 (1) ◽  
pp. 7-14
Author(s):  
Turgut Taşkın ◽  
Eray M. Güler ◽  
Şeyda Şentürk ◽  
Damla D. Çelik ◽  
Turan Arabacı ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Antimicrobial Agents ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Cytotoxic Activities ◽  
Active Compounds ◽  
Antioxidant Power ◽  
Biological Potential

Background: The genus Achillea is one of the most important genus of the Asteraceae family and many species of Achillea are used in traditional medicine to treat several ailments. Aim: The aim of the current research was to evaluate in vitro cytotoxic activities of n-hexan, chloroform, ethyl acetate and methanol extracts and to isolate the active compounds from the extract showing the strongest cytotoxic activity. In addition to this, it was aimed to evaluate the biological activities (cytotoxic, antioxidant, anti-urease, anticholinesterase, antimicrobial) of different extracts and active compounds from Achillea monocephala. Methods and Materials: The in vitro antioxidant, cytotoxic, anti-urease, anticholinesterase and antimicrobial activities of different extracts from A. monocephala aerial parts were examined. The structures of the active compounds were determined by NMR techniques, UV, IR and LC-MS/MS analysis and their biological potential was examined. Results: The chloroform extract showed strong and selective cytotoxic activity on the cancer cell lines (MDA-MB-231, MCF-7). Besides, this extract exhibited stronger antimicrobial activity than other extracts. Therefore, through activity-guided procedures, luteolin, naringenin and 8-hydroxy-salvigenin compounds were isolated from this extract. The methanol extract showed stronger antioxidant (DPPH, ABTS, CUPRAC) and anticholinesterase activity than other extracts. The n-hexan extract exhibited the highest anti-urease activity. In this study, it was determined that the isolated compounds had a strong biological activity. Naringenin compound had stronger ABTS radical cation scavenging and ferric reducing/antioxidant power, cytotoxic and antimicrobial activity than other compounds. 8-hydroxy-salvigenin compound showed the highest urease and acetylcholinestease enzyme inhibition. Conclusion: The results of this study suggest that the extracts and isolated compounds from the A. monocephala may be used as antioxidant, cytotoxic, anti-urease, anticholinesterase and antimicrobial agents in the future.

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