scholarly journals Does the Use of a “Walking Bleaching” Technique Increase Bone Resorption Markers?

2018 ◽  
Vol 43 (3) ◽  
pp. 250-260 ◽  
Author(s):  
C Bersezio ◽  
P Vildósola ◽  
M Sáez ◽  
F Sánchez ◽  
R Vernal ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Root Resorption ◽  
Gingival Crevicular Fluid ◽  
Good Condition ◽  
Study Groups ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carbamide Peroxide ◽  
Tooth Color ◽  
Periodontal Tissues ◽  
Crevicular Fluid

SUMMARY Objective: This randomized clinical trial evaluated the effect of 35% hydrogen peroxide in comparison with 37% carbamide peroxide in a nonvital bleaching technique of “walking bleaching” (four sessions of treatment) on periodontal markers: nuclear factor kappa B-ligand (RANK-L—process of root resorption marker) and interleukin 1β (IL-1β—inflammatory response marker). Methods and Materials: Fifty volunteers presenting with discoloration of nonvital teeth and endodontic treatment in good condition participated. Fifty teeth were randomly divided into two study groups according to bleaching gel: HP = 35% hydrogen peroxide (n=25) and 37% carbamide peroxide (n=25). Nonvital bleaching was performed with a walking bleaching technique consisting of four sessions of bleach application. Gingival crevicular fluid samples were taken in order to quantify the RANK-L and IL-1β levels by enzyme-linked immunosorbent assay. Samples were obtained from six periodontal sites for each bleached tooth: three vestibular and three palatine (mesial, middle, and distal) at seven time periods: baseline, after each of the four sessions of nonvital bleaching, at one week, and at one month after nonvital bleaching. Tooth color variations were analyzed in each session by VITA Bleachedguide 3D-MASTER (ΔSGU). Results: Significant increments in the RANK-L and IL-1β levels were detected in each evaluated time compared with baseline (p<0.05); however, no differences were detected between hydrogen peroxide and carbamide peroxide on increments of the biomarkers studied. The change of color was effective for both nonvital bleaching therapies (p<0.05). Conclusions: Nonvital bleaching induced a significant increment in the RANK-L and IL-1β levels in periodontal tissues around bleached, nonvital teeth.

scholarly journals WNT-5a and SOST Levels in Gingival Crevicular Fluid Depend on the Inflammatory and Osteoclastogenic Activities of Periodontal Tissues

Medicina ◽  
2021 ◽  
Vol 57 (8) ◽  
pp. 788
Author(s):  
Georgios S. Chatzopoulos ◽  
Massimo Costalonga ◽  
Kim C. Mansky ◽  
Larry F. Wolff
Keyword(s):  
Wnt Signaling ◽  
Gingival Crevicular Fluid ◽  
Diagnostic Category ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Periodontal Tissues ◽  
Receptor Activator ◽  
Crevicular Fluid ◽  
Periodontal Tissue Remodeling

Background and Objectives: Wnt signaling leads to stimulation of osteoblasts and it reduces osteoclastogenesis and bone resorption via the regulation of the osteprotegrin and receptor activator of nuclear factor kappa-Β ligan (RANKL). Wnt signaling pathways are regulated by their physiological antagonists such as sclerostin (SOST) as well as WNT-5a. The aim of this study was to determine the total amount of Sclerostin and WNT-5a in the gingival crevicular fluid (GCF) in sites with a continuum from a healthy to diseased periodontium. Materials and Methods: In this cross-sectional study, a total of 20 patients with generalized periodontitis, 10 subjects with gingivitis as well as 14 individuals with a healthy periodontium were recruited upon clinical and radiographic periodontal examination. In patients diagnosed with periodontitis, GCF samples were collected from periodontitis, gingivitis and healthy sites, while gingivitis patients provided samples from gingivitis and healthy sites. In healthy patients, only healthy sites were sampled. Protein total amount of SOST and WNT-5a were quantified by sandwich enzyme-linked immunosorbent assay (ELISA). Results: A total of 108 GCF samples were collected from a total of 44 individuals. When all periodontitis (n = 51), gingivitis (n = 12) and healthy (n = 45) sites were analyzed regardless of the patient diagnosis, periodontitis sites demonstrated significantly elevated WNT-5a total amounts (p = 0.03) when compared to gingivitis sites. Gingivitis sites demonstrated a trend of more total SOST (p = 0.09) when compared to periodontitis and healthy sites. Within each patient diagnostic category, sites showed similar SOST and WNT-5a total amounts (p > 0.05). Conclusions: WNT-5a levels in GCF depend on the stage of periodontitis sites. SOST trended higher in the GCF of gingivitis sites but similar in chronic periodontitis and healthy sites. WNT-5a and SOST play a crucial role in periodontal tissue remodeling and depend on the inflammatory and osteoclastogenic activities.

scholarly journals Biomarker for Asymptomatic Apical Periodontitis in Gingival Crevicular Fluid: aMMP-8

2020 ◽  
Vol 14 (02) ◽  
pp. 239-244 ◽  
Author(s):  
Teodora Karteva ◽  
Neshka Manchorova-Veleva
Keyword(s):  
Gingival Crevicular Fluid ◽  
Apical Periodontitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cross Sectional ◽  
Significance Level ◽  
Periodontal Tissues ◽  
Diagnostic Potential ◽  
Potential Biomarker ◽  
Crevicular Fluid

Abstract Objectives Asymptomatic apical periodontitis (AAP) is one of the most widespread chronic inflammatory diseases in the field of dental medicine. Active matrix metalloproteinase (aMMP-8) previously demonstrated diagnostic potential as a biomarker for AAP in gingival crevicular fluid (GCF). The aim of this study was to determine the levels and diagnostic accuracy of aMMP-8 in GCF from teeth with AAP. Materials and Methods In this cross-sectional study, GCF samples were obtained from teeth with AAP (sample group, n = 31) and their contralateral teeth (control group, n = 31). Clinical and cone beam computed tomography (CBCT) parameters were used for the diagnosis and assessment of AAP and the determination of clinically healthy marginal periodontal tissues. One pool GCF sample per tooth was obtained from the whole crevice’s perimeter. aMMP-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). Statistical Analysis Wilcoxon signed ranks test and Spearman rank correlation coefficient (rs) were used as statistical tools. The significance level was set at p < .05. Results The two groups demonstrated biomarker levels corresponding to a healthy marginal periodontal tissue. aMMP-8 levels were statistically and significantly higher in the samples collected from teeth with AAP. Lesions with greater volume showed correspondingly larger diameters. No statistically significant correlation between aMMP-8 levels and lesions’ volume or diameter was discovered. Conclusion GCF composition is modified by AAP only to a minimal extent. Further research is needed to substantiate the utilization of aMMP-8 as a potential biomarker for the diagnosis of the disease as well as to explore its relationship with other biomarkers.

scholarly journals Levels of matrix metalloproteinase-7 and osteopontin in human gingival crevicular fluid during initial tooth movement

2015 ◽  
Vol 5 ◽  
pp. 77-82 ◽  
Author(s):  
Dhaval Oswal ◽  
Ravindranath B. Sable ◽  
Amol Patil ◽  
Alpana Moge ◽  
Shama Aphale
Keyword(s):  
Matrix Metalloproteinase ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Peak Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Time Interval ◽  
Human Teeth ◽  
Orthodontic Force ◽  
Periodontal Tissues ◽  
Crevicular Fluid

Purpose During orthodontic treatment, the early response of periodontal tissues to mechanical stress involves several metabolic changes that allow tooth movement. The purpose of this investigation was to evaluate osteopontin (OPN) and matrix metalloproteinase (MMP)-7 in the gingival crevicular fluid (GCF) of human teeth exposed to orthodontic force. Materials and Methods GCF samples were obtained from 15 healthy orthodontic patients (age, 12-22 years). In each patient, the left maxillary canine having the fixed orthodontic appliance was used as the test tooth, and its antagonist, with no appliance, was the control. Orthodontic force, 75 g was applied using a 16 × 22 beta titanium closing loop. The GCF sampling on the disto-buccal aspects of experimental and control tooth was performed at specific time interval with sterilized absorbent paper point. Processing was carried out with enzyme-linked immunosorbent assay to detect OPN and MMP-7 levels. Results The peak level of OPN was seen after 1 h application of orthodontic force which was 1280.36 pg/ml ± 185.02. The peak level of MMP-7 was seen at 0 h which was 598.3 pg/ml ± 107.5. The levels of OPN after 1 h increased to 1280.36 pg/ml ± 185.02, and they decreased at 24 h to 1012.86 pg/ml ± 168.47 (P = 0.001). The levels of MMP-7 after 1 h decreased to 478 pg/ml ± 99.7 which increased at 24 h to 526.9 pg/ml ± 99.2. Conclusions Orthodontic forces affect both OPN and MMP-7 protein levels on the compression side in a time-dependent fashion.

scholarly journals Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

2021 ◽  
Vol 11 (2) ◽  
pp. 521
Author(s):  
Simina Chelărescu ◽  
Petra Șurlin ◽  
Mioara Decusară ◽  
Mădălina Oprică ◽  
Eugen Bud ◽  
...  
Keyword(s):  
Young Adults ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Early Stage ◽  
Statistical Tests ◽  
Orthodontic Tooth Movement ◽  
Adolescents And Young Adults ◽  
Periodontal Tissues ◽  
Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

scholarly journals Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Oya Türkoğlu ◽  
Elif Azarsız ◽  
Gülnur Emingil ◽  
Necil Kütükçüler ◽  
Gül Atilla
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Study Groups ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Proteinase 3 ◽  
Cathepsin C ◽  
Probing Depth ◽  
Inflammatory Processes ◽  
Crevicular Fluid

Aim. Cathepsin C is the activator of the polymorphonuclear leukocyte-derived proteinase 3, which contributes to inflammatory processes. The aim of the present study was to investigate gingival crevicular fluid (GCF) proteinase 3 and cathepsin C levels in periodontal diseases.Design. Eighteen patients with chronic periodontitis (CP), 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with gingivitis, and 18 healthy subjects were included in the study. Periodontal parameters including probing depth, clinical attachment level, papilla bleeding index, and plaque index were assessed in all study subjects. GCF proteinase 3 and cathepsin C levels were analyzed by ELISA.Results. GCF proteinase 3 total amount was significantly higher in diseased groups compared to control group, after adjusting ageP<0.05. No differences were found in GCF cathepsin C levels among the study groupsP>0.05. Periodontal parameters of sampling sites were positively correlated with GCF proteinase 3 total amountsP<0.01but not with cathepsin C total amountsP>0.05.Conclusions. Elevated levels of GCF proteinase 3 in CP, G-AgP, and gingivitis might suggest that proteinase 3 plays a role during inflammatory periodontal events in host response. However, cathepsin C in GCF does not seem to have an effect on the pathogenesis of periodontal diseases.

scholarly journals The Effects of Orthodontic Forces during Canine Retraction Using Self-ligating Brackets on Gingival Crevicular Fluid Enzyme Activity, Canine Movement and Root Resorption

2015 ◽  
Vol 44 (2) ◽  
pp. 249-256 ◽  
Author(s):  
Rohaya Megat Abdul Wahab ◽  
Zulham Yamamoto ◽  
Albira Sintian ◽  
Nurfathiha Abu Kasim ◽  
Intan Zarina Zainol Abidin ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Root Resorption ◽  
Gingival Crevicular Fluid ◽  
Canine Retraction ◽  
Crevicular Fluid ◽  
Orthodontic Forces

scholarly journals Combination of at-home and in-office bleaching techniques: case series

2021 ◽  
Vol 69 ◽  
Author(s):  
Marcela Alvarez FERRETTI ◽  
Matheus KURY ◽  
Beatriz Curvello de MENDONÇA ◽  
Marcelo GIANNINI ◽  
Vanessa CAVALLI ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Case Series ◽  
Tooth Bleaching ◽  
Central Incisor ◽  
Carbamide Peroxide ◽  
Tooth Sensitivity ◽  
Tooth Color ◽  
Shade Guide ◽  
At Home

ABSTRACT Tooth bleaching is one of the most conservative aesthetic techniques. At-home and in-office bleaching may be performed, as well as the combination of both techniques. As this combination may be done in different manners, distinct orders of combinations were proposed in this report. A 24 years-old man, whose upper central incisor and canine shades were A2 and A3 respectively, was initially treated by chair-side with 40-min application of 40% hydrogen peroxide (HP) for two sessions. Afterwards, an two-week overnight at-home bleaching was performed with 10% carbamide peroxide (CP). The shade of upper central incisors changed to 1M1 and canine was B1. Conversely, a 30 years-old woman was firstly conducted with at-home bleaching (overnight 4-weeks 10% CP) and, subsequently, with a single appointment of in-office bleaching for 45 minutes (35% HP). The shade of her upper central incisor changed from A1 to 0.5M1 and the upper canine from A3 to B1. The VITA classical (A1-D4) shade guide and VITA Bleached guide 3D-MASTER were used to determine the tooth color during the treatments. In both bleaching treatments, patients reported no significant tooth sensitivity and the final outcomes met their expectation.

Six-month Follow-up of the Effect of Nonvital Bleaching on IL-1β and RANK-L: A Randomized Clinical Trial

2019 ◽  
Vol 44 (6) ◽  
pp. 581-588 ◽  
Author(s):  
C Bersezio ◽  
J Estay ◽  
M Sáez ◽  
F Sánchez ◽  
R Vernal ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Hydrogen Peroxide ◽  
Randomized Clinical Trial ◽  
Gingival Crevicular Fluid ◽  
Color Change ◽  
Double Blind ◽  
Time Points ◽  
Periodontal Tissues ◽  
Significant Difference ◽  
Spearman Test

SUMMARY Objectives: It has been reported that bleaching generates an increase in the activity of osteoclasts in vitro. We quantified the RANK-L and IL-1β biomarkers in a double-blind, randomized clinical trial evaluating the in vivo effect of hydrogen peroxide (35%) and peroxide carbamide (37%) six months after whitening. Methods and Materials: Fifty volunteers participated, each with color change in a nonvital tooth. Fifty teeth were randomly divided into two groups (n=25), and the teeth were bleached using either 35% hydrogen peroxide (G1) or 37% carbamide peroxide (G2). Intracoronal bleaching was carried out by a technical “walking bleach” over four sessions. Gingival crevicular fluid samples were collected and used to quantify the IL-1β and RANK-L secreted levels. Samples of six periodontal sites (three vestibular and three palatal) were collected for up to six months (at the beginning of the study [baseline] and at one week, one month, and six months posttreatment). The color change was visually monitored using the Vita Bleached Guide (ΔSGU). Results: Comparing each time to baseline assessment, a significant increase in the levels of IL-1β and RANK-L across time points was detected (p&lt;0.05). The color change was 4 in G1 and G2, and a statistically significant difference (p&lt;0.05) was found at the month time point between the groups. Using the Spearman test, a strong correlation (&gt;0.8) between the IL-1β and RANK-L levels in both groups at all time points was detected. Conclusions: Nonvital bleaching using a technical walking bleach induces an increase in the IL-1β and RANKL production in periodontal tissues, which persists for six months after treatment. Both biomarkers were highly correlated in both groups and at all time points.

scholarly journals CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Claudia Dellavia ◽  
Elena Canciani ◽  
Giulio Rasperini ◽  
Giorgio Pagni ◽  
Matteo Malvezzi ◽  
...  
Keyword(s):  
Surgical Procedures ◽  
Early Phase ◽  
Gingival Crevicular Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wound Fluid ◽  
Protein Levels ◽  
Inflammatory Phase ◽  
Crevicular Fluid ◽  
Open Flap Debridement ◽  
Periodontal Healing

Objectives. Cementogenesis seems to be significantly compromised during tissue inflammation. In dental practice, surgical procedures are performed with the aim to regenerate periodontium including cementum. However, inflammation that occurs during the initial healing phases after surgery may impair regeneration of this tissues. The aim of the present study was to assess if surgical procedures designed to regenerate periodontium might affect levels of cementum protein-1 (CEMP-1) in periodontal wound fluid during early phase of healing. Materials and Methods. In 36 patients, 18 intrabony periodontal defects were treated with regenerative therapy (REG group) and 18 suprabony periodontal defects were treated with open flap debridement (OFD group). In the experimental sites, gingival crevicular fluid was collected immediately before surgery, and periodontal wound fluid was collected 4, 7, 14, and 21 days after surgery. CEMP-1 levels were detected by indirect enzyme-linked immunosorbent assay technique. Results. At the analysis, it resulted that there was a significant average difference in CEMP-1 values between the REG and OFD groups at baseline (p=0.041), the CEMP-1-modeled average in the OFD group was lower by 0.45 ng/ml. There was a significant trend in CEMP-1 over time, and this trend was different among the 2 groups: the REG group showed a statistically significant rising CEMP-1 trend (0.18 ng/ml a week p=0.012), while the OFD had a trend that was significantly lower (-0.22 ng/ml a week compared to the REG group trend p=0.023), the OFD group lost on average 0.05 ng/ml a week. In REG sites, GCF protein levels resulted also related to clinical parameters. Conclusions. During the initial inflammatory phase of periodontal healing, CEMP-1 levels decrease regardless of the surgical protocol applied. The surgical procedures used to regenerate periodontal tissue are able to reverse this trend and to induce significant increase of CEMP-1 in periodontal wound fluid after the first week postop.

Sign in / Sign up

Export Citation Format

Share Document