scholarly journals Modulation of BTEX Bio - Remedial Activity by Molecular Triggering from Facultative Anaerobic Activity

2019 ◽  
Vol 4 (2) ◽  
pp. 1-9
Author(s):  
Chang HD
Keyword(s):  
Humic Acid ◽  
Carbon Source ◽  
High Efficiency ◽  
Lipid Membrane ◽  
Reducing Power ◽  
Carbon Chain ◽  
Ferrous Ions ◽  
Facultative Anaerobic ◽  
Acti Vity ◽  
One Year

BTEX (Benzene, Toluene, Ethylbenzene, Xylene) is widely reside in fuel and painting process, such as industrial metal painting. Due to their volatile character, it is easily emitted and recalcitrant in disintegration through aromatic property. These vapor inhaled and slow corruption the lipid membrane of the lungs tissue. Benzene and derivatives are thus more mutagenic and carcinogenic evaluated. The leakage of oil and spoil the soil and groundwater which arouse publi c concern about health respect. Therefore, it is crucial to develop high - efficiency of pollutant transformation, with aims to instant treatment of their toxicity or start remediation process. This study had screened several active isolates from bio - fixed b iofilms consortia were carried. The investigation concerns the supplement of electronic molecules for the remediation effect of BTEX were tried and the cultivation status of facultative anaerobic state were addressed. In highlight of finding with high acti vity consortium was enriched from humic acid eluted from compost of kitchen waste, add additional 400 mg/L BTEX compound over almost one year. Active isolate were compared by using different carbon source as electron donors with 31.2 μM substrate concentra tion. After the basal carbon source as humic acid and molasses was extra added and compared, remedial activity of the strain with 3 - 3> 3 - 1> 2 - 6 were compared. As a second level analysis, the remedial effects shown: methanol>glucose>indole acetic acid>indol e, indicated that the electromotive force might influenced by the grafting carbon chain, the length of carbon grafting, the influence of reducer as ferrous ions in medium, also the free benzoic compound interacted, dynamic directing the direction of electr on flux from humic acid or molasses, overall, humic acid, generally less conversion, believed only support electron mediation in other reaction. While molasses, macromolecular and exhibits a reducing power with glucose and methanol. However, it causes inva lid electronic flux into aromatic conversion but competitively switch to oxidative respiration. Resulted an invalid co - metabolism. The significant of remedial effect by grafting aromatics and soluble humic acid were demonstrated in this report.

Antioxidant Activity, Phenolic Content and Hematoprotective Effects of Cleome arabica Polyphenolic Leaf Extract

2019 ◽  
Author(s):  
C. Tigrine ◽  
A. Kameli
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Reducing Power ◽  
Hematological Toxicity ◽  
Protective Effects ◽  
Ferrous Ions ◽  
Polyphenolic Extract ◽  
Cleome Arabica

In this study a polyphenolic extract from Cleome arabica leaves (CALE) was investigated for its antioxidant activity in vitro using DPPH•, metal chelating and reducing power methods and for its protective effects against AraC-induced hematological toxicity in vivo using Balb C mice. Results indicated that CALE exhibited a strong and dose-dependent scavenging activity against the DPPH• free radical (IC50 = 4.88 μg/ml) and a high reducing power activity (EC50 = 4.85 μg/ml). Furthermore, it showed a good chelating effects against ferrous ions (IC50 = 377.75 μg/ml). The analysis of blood showed that subcutaneous injection of AraC (50 mg/kg) to mice during three consecutive days caused a significant myelosupression (P < 0.05). The combination of CALE and AraC protected blood cells from a veritable toxicity. Where, the number of the red cells, the amount of hemoglobin and the percentage of the hematocrite were significantly high. On the other hand, AraC cause an elevation of body temperature (39 °C) in mice. However, the temperature of the group treated with CALE and AraC remained normal and did not exceed 37.5 °C. The observed biological effects of CALE, in vitro as well as in vivo, could be due to the high polyphenol and flavonoid contents. In addition, the antioxidant activity of CALE suggested to be responsible for its hematoprotective effect.

scholarly journals Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

2011 ◽  
pp. 279-288 ◽  
Author(s):  
Anita Klaus ◽  
Maja Kozarski ◽  
Miomir Niksic
Keyword(s):  
Antioxidant Activity ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Reducing Power ◽  
Hot Water ◽  
Ferrous Ions ◽  
Medicinal Mushrooms ◽  
Water Extracts ◽  
Hot Water Extract

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ? 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ? 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ? 0.1 at 20 mg/ml, and 0.3 ? 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ? 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ? 2.5% at 10 mg/ml. Gl-I chelated 81.6 ? 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ? 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

Bioavailability of a nonylphenol isomer in dependence on the association to dissolved humic substances

2004 ◽  
Vol 50 (5) ◽  
pp. 277-283 ◽  
Author(s):  
R. Vinken ◽  
A. Höllrigl-Rosta ◽  
B. Schmidt ◽  
A. Schäffer ◽  
P.F.-X. Corvini
Keyword(s):  
Humic Acid ◽  
Carbon Source ◽  
Humic Substances ◽  
Organic Compounds ◽  
Sole Carbon Source ◽  
Equilibrium Dialysis ◽  
Fulvic Acids ◽  
Degrading Bacterium ◽  
Bacterium Strain ◽  
Dissolved Humic Substances

Humic substances are important environmental components since they represent a very large part of organic compounds on earth. According to many reports, dissolved humic substances are a determinant parameter for the bioavailability of xenobiotic compounds. For the present bioavailability studies, two kinds of dissolved humic substances, a commercially available humic acid and fulvic acids isolated from peat were used. As the relevant xenobiotic, a defined branched nonylphenol isomer, 4(3′,5′-dimethyl-3′-heptyl)-phenol (p353NP) was synthesised according to Friedel-Crafts alkylation. Equilibrium dialysis studies were implemented in order to investigate the association between 14C-labelled p353NP and dissolved humic substances. The biodegradability in the presence of dissolved humic substances was examined in experiments with the nonylphenol degrading bacterium strain Sphingomonas TTNP3 and with p353NP as sole carbon source. The results showed that p353NP-humic acid associates were formed in high amounts, whereas no adducts with fulvic acids occurred. In the degradation studies with Sphingomonas TTNP3, no effects of dissolved humic substances on the bioavailability of p353NP could be observed. It was assumed that the association between nonylphenol and humic acids occurs rapidly and is reversible. Thus, the formation of "labile" complexes did not influence biodegradation rates, which were quite low.

scholarly journals Antioxidant Activity and Total Phenolic Content in Shiitake Mycelial Exudates

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Weihuan Huang ◽  
Joo-Shin Kim ◽  
Hau Yin Chung
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Shiitake Mushroom ◽  
Ferrous Ions

Exudates (DE) secreted from two shiitake mushroom mycelia (strains 1358 and L5458) were evaluated for their antioxidative properties and phenolic content. 1358DE and L5458DE showed distinct antioxidant activity in different in vitro assays, including scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radical, superoxide anions and hydrogen peroxide; the ability to chelate ferrous ions; reducing power; hemolysis inhibition activity in rat erythrocyte; and lipid peroxidation inhibition (IC50 values of 1358DE and L5458DE were 3.3 and 132.6; 44.5 and > 1000; 26.9 and 53.7; 153.6 and >175.0; 176.0 and 521.0; 26.7 and 746.4; 47.8 and 736.9; and 3.1 and > 1000 μg/mL, respectively). Their total phenolic content was 237.33 and 24.08 mg gallic acid equivalent (GAE)/g of dry DE, respectively. Overall, these results show that 1358DE generally possesses better antioxidant properties than L5458DE, possibly due to its larger total phenolic content. Shiitake mushroom mycelial exudates, particularly of 1358DE, could be a good source of natural antioxidants.

scholarly journals Analgesic and Antioxidant Activities of 4-Phenyl-1,5-benzodiazepin-2-one and Its Long Carbon Chains Derivatives

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Terence Nguema Ongone ◽  
Redouane Achour ◽  
Mostafa El Ghoul ◽  
Latyfa El Ouasif ◽  
Meryem El Jemli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Carbon Chain ◽  
Immersion Test ◽  
Tail Immersion ◽  
Reducing Power Assay

The aim of this work is to deepen the pharmacological effect of 4-phenyl-1,5-benzodiazepin-2-one derivatives which have a similar structure to nonionic surfactants: 4-phenyl-1,5-benzodiazepin-2-one is the hydrophilic head, and the carbon chain is hydrophobic tail. The antinociceptive activity of 4-phenyl-1,5-benzodiazepin-2-one derivatives was determined using acetic acid-induced writhing and tail immersion tests. In addition, the in vitro antioxidant activities of the tested derivatives were determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferric reducing power assay. A single oral administration of these compounds at the doses of 50 and 100 mg/kg significantly reduced the number of abdominal writhes induced by acetic acid injection. Acute pretreatment with 4-phenyl-1,5-benzodiazepin-2-one derivatives at the dose of 100 mg/kg caused a significant increase in the tail withdrawal latency in the tail immersion test. Additionally, a significant scavenging activity in DPPH and reducing power was observed in testing antioxidant assays. Finally, we carried out a study of the antioxidant activity of these derivatives. The results of this study reveal that these compounds have a low antioxidant activity compared to the BHT. It decreases with the polarity of the molecule. The present study suggests that 4-phenyl-1,5-benzodiazepin-2-one derivatives possess potent antinociceptive and antioxidant effects, which suggest that the tested compounds may be useful in the treatment of pain and oxidation disorders.

scholarly journals Biological Treatment Of Simulated Humic Acid Wasre Water In A Laboratory Scale Aerobic Reactor

REAKTOR ◽  
2017 ◽  
Vol 7 (1) ◽  
pp. 33
Author(s):  
Yunardi Yunardi
Keyword(s):  
Humic Acid ◽  
Carbon Source ◽  
Activated Sludge ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Domestic Wastewater ◽  
Ammonium Nitrogen ◽  
Synthetic Wastewater ◽  
Waste Water Treatment Plant ◽  
Organic Carbon Concentration

A laboratory study was  conducted to determine the feasibility of activated sludge reactor for treating humic acid waste waterand examine the effect of the presence of glucose as the second carbon source on treatment performance. Activated sludge obtained from a waste water treatment plant treating domestic wastewater was used as a seed for reactors. Synthetic wastewater containing humid acid as the sole carbon source and glucose as the second carbon source were used as feeds for the reactors operated in a fill-and-draw mode. The result showed  tht the presence of glucose in the wastewater enhanced the production of higher MLSS (mixed liquor suspended solids) than that without of glucose. The TOC (Total Organic Carbon) concentration in both reactors fluctuated greatly until the end of the experiment due to inability of keeping the input TOC at desired level. However the decrease in TOC suggested that there are some microorganisms capable of degrading the humic acid. Addition of glucose to the simulated humic acid wastewater improved the capability of microbes in degrading the acids. Most ammonium nitrogen in the wastewater was converted to nitrate nitrogen. Although the performance was lower compared to that of conventional system, activated sludge process was capable of degrading wastewater containing humic acids.Keywords : activated sludge, ammonium nitrogen, fill and draw, glucose, humic acid, wastewater

Biological Groundwater Denitrification: Laboratory Studies

1988 ◽  
Vol 20 (3) ◽  
pp. 189-195 ◽  
Author(s):  
M. I. M. Soares ◽  
S. Belkin ◽  
A. Abeliovich
Keyword(s):  
Carbon Source ◽  
Marked Decrease ◽  
Vacuum Treatment ◽  
Partial Recovery ◽  
Column Efficiency ◽  
Laboratory Studies ◽  
Sand Column ◽  
Carbon To Nitrogen Ratio ◽  
Nitrogen Ratio ◽  
One Year

Laboratory denitrification studies were conducted in a sand column using sucrose as a biodegradable carbon source. The denitrification capacity of the system was followed for more than one year. Column efficiency was dependent on the flow rate and on the carbon to nitrogen ratio in the treated water. After a few months of operation, visible accumulation of gas in the active zone was accompanied by a marked decrease in column permeability. Vacuum treatment restored permeability to its original level; only partial recovery was achieved by simply allowing the slow natural release of gas. He suggest that under the conditions tested, clogging resulted from the accumulation of microbiologically produced gas.

scholarly journals Surveying Denitrification Efficacy in Up-Flow Packed Bed Bioreactor Operated under Heterotrophic Condition Using Autotrophic Bacteria

2019 ◽  
Author(s):  
Ali Asghar Neshat ◽  
Abdomajid Gholizadeh ◽  
Babak Jahed ◽  
Pouria Nikvand
Keyword(s):  
Acetic Acid ◽  
Carbon Source ◽  
High Efficiency ◽  
Nitrate Removal ◽  
Packed Bed ◽  
Critical Issue ◽  
Pilot Scale ◽  
Aqueous Environment ◽  
Packed Bed Bioreactor ◽  
Autotrophic Bacteria

Introduction: The biological denitrification process is an interesting cost-effective technique to remove nitrate from water supplies. Acetic acid can be used as a carbon source in this process, but its consumption rate is a critical issue and, in some cases, it is quite different from stoichiometric constants. The current study aimed to investigate the nitrate removal in an up-flow packed bed bioreactor. Furthermore, various parameters affecting this process were investigated and optimized. In this study, the autotrophic bacteria were used for the heterotrophic process. Materials and Methods: Initially, the autotrophic bacteria were cultured and used for the following heterotrophic conditions in distinct reactors. A pilot-scale anoxic up flow bioreactor packed was constructed using the polyethylene media and applied to remove nitrate from the aqueous environment. Consequently, the effects of hydraulic retention times (HRT) and different acetic acid concentrations as carbon source were evaluated. During the study, the amounts of alkalinity, pH, temperature, and nitrate were checked. Results: The designed bioreactor removed an average of over 88% of nitrate, while the acetic acid consumption was 2 mg/mg NO3-N, which was lower than the stoichiometric constant for heterotrophic process. Moreover, in the three studied HRTs (1.5, 3, and 5 h), the Alkalinity increased from 14.2 to 19.8 %. Conclusion: The results of this study showed high efficiency in nitrate removal via heterotrophic denitrification using acetic acid as carbon source for autotrophic bacteria.

scholarly journals Efficiency of Photocatalytic Degradation of Humic Acid Using Magnetic Nanoparticles (Fe-doped TiO2@Fe3O4) in Aqueous Solutions

Health Scope ◽  
2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Hossein Moein ◽  
Gholamreza Nabi Bidhendi ◽  
Naser Mehrdadi ◽  
Hossein Kamani
Keyword(s):  
Magnetic Nanoparticles ◽  
Humic Acid ◽  
Aqueous Solutions ◽  
Photocatalytic Degradation ◽  
High Efficiency ◽  
Sol Gel ◽  
Reaction Times ◽  
Doped Tio2 ◽  
Natural Organic Matters ◽  
Uv Lamp

Background: Among water pollutants, Natural Organic Matters (NOMs) are highly important for making problems in water treatment plants. Objectives: The main objective of this study was to investigate the efficiency of photocatalytic degradation of humic acid using magnetic nanoparticles (Fe-doped TiO2@Fe3O4) in aqueous solutions. Methods: In the present experiment, Fe-doped TiO2@Fe3O4 nanoparticles were synthesized by the sol-gel method, and SEM, XRD, and DRS analyzes were utilized to characterize the synthesized nanoparticles. The effects of various variables, including pH (3 - 11), initial concentration of humic acid (20 - 80 mg/L), and concentration of nanoparticles (250 - 2000 mg/L) at different reaction times (15 - 60 min) were investigated on the photocatalytic degradation of humic acid. Results: The maximum degradation efficiency of humic acid at pH 3, the initial humic acid concentration of 5 mg/L, nanoparticle dose of 400 mg/L, and reaction time of 60 min using a 15-W bare UV lamp. Conclusions: Due to the high efficiency of photocatalytic degradation, it is proposed to use for the removal of humic acid from water resources.

scholarly journals Phytochemical Characterization, Antioxidant and In Vitro Cytotoxic Activity Evaluation of Juniperus oxycedrus Subsp. oxycedrus Needles and Berries

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 502 ◽  
Author(s):  
Reda Ben Mrid ◽  
Najat Bouchmaa ◽  
Youssef Bouargalne ◽  
Btissam Ramdan ◽  
Khalid Karrouchi ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reactive Oxygen Species Generation ◽  
Reducing Power ◽  
Breast Cancer Cell Lines ◽  
Ferrous Ions ◽  
Cytotoxic Effects ◽  
Juniperus Oxycedrus

In order to evaluate the antioxidant properties of aqueous and methanol extracts of needles and berries of Juniperus oxycedrus subsp. oxycedrus (Joo) species, various antioxidant capacity assessment tests (free radical scavenging assays (DPPH• and ABTS•+ tests), ferrous ions (Fe2+) chelating activity and reducing power assay (FRAP) were conducted. In all of the tests, the extracts exhibited strong antioxidant activity. Furthermore, in-vitro cytotoxic activity assays of the methanolic extracts showed potent cytotoxic effects against two breast cancer cell lines (MDA-MB-468 and MCF-7), with no cytotoxicity towards normal cells (PBMCs). Reactive oxygen species generation was presumed to be a potential reason for the observed cytotoxic effects. According to all the above, and considering its appropriate composition of mineral elements and phenolic compounds, Joo could offer a beneficial and natural source of bioactive compounds that can be either used on the preventive side as it could potentially be used in the clinic without toxicity.

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