scholarly journals Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women

2020 ◽  
Vol 5 (4) ◽  
pp. 38-45
Author(s):  
A. V. Shabaldin ◽  
N. S. Deeva ◽  
A. S. Sukhikh ◽  
G. V. Vavin
Keyword(s):  
Functional Activity ◽  
Protein Concentration ◽  
Heart Defects ◽  
Peripheral Blood Lymphocytes ◽  
Reproductive Age ◽  
Immune Disorders ◽  
Hla Dr ◽  
Multiparous Women ◽  
Donor Lymphocytes ◽  
Apparently Healthy

Aim. To obtain a purified IgG preparation from the plasma of multiparous women and to evaluate its functional activity towards HLA-DR and HLA-G molecules.Materials and Methods. IgG preparation was prepared by means of affinity chromatography using the DEAE Affi-Gel Blue system. The purity of the isolated preparation was assessed by immunoelectrophoresis. Functional activity of the IgG preparation against HLA-DR and HLA-G molecules was assessed in peripheral blood lymphocytes isolated from 14 apparently healthy men of reproductive age by flow cytometry.Results. A 30 mL IgG fraction was obtained with a protein concentration of 4.3 g/l and a residual albumin less than 0.1 g/l. The protein concentration in the obtained fraction corresponded to the lower limit of IgG concentration in human serum. Immunoelectrophoresis showed that the IgG was the only antibody fraction in the chromatographic washout. Purified IgG fraction suppressed the expression of HLA-DR and HLA-G in donor lymphocytes.Conclusion. The purified IgG fraction from multiparous women can have a significant preventive and therapeutic effect against immune disorders in the mother-embryo system and therefore might halt the development of congenital heart defects by blocking HLA-DR and HLA-G in the lymphocytes.

Experience of using chlorhexidine in obstetrics and gynecology

2018 ◽  
pp. 46-50
Author(s):  
O.V. Kravchenko ◽  
Keyword(s):  
Pregnant Women ◽  
Key Words ◽  
Functional Activity ◽  
High Efficiency ◽  
Reproductive Age ◽  
Obstetrics And Gynecology ◽  
Laboratory Examination ◽  
Control Groups ◽  
Treatment Results ◽  
Regenerative Activity

The objective: generalization of the experience of using chlorhexidine drugs in obstetric and gynecological practice. Materials and methods. The efficacy of using Depantol and Hexicon for non-specific colpitis in patients of adolescent, reproductive age, pregnant women, women in perimenopause, as well as in patients after cervical, vaginal and perineal operations was evaluated and generalized. The surveyed groups contained 30 to 50 patients. The diagnosis of nonspecific vaginitis was verified by clinical and laboratory examination. Criteria for recovery were complete clinical and bacterial sanation. The effectiveness of therapy was evaluated immediately after treatment and after 1 and 3 months. The data were compared with the appropriate control groups or with the condition prior to treatment. Results. High efficiency of chlorhexidine preparations in the treatment of nonspecific colpitis in patients of different age categories and conditions was noted. The experience of using Depantol and Gecikon in obstetrics and gynecology is generalized, priorities are determined in the application of these drugs. Conclusion. Preparations of chlorhexidine Geksikon and Depantol are highly effective against the main pathogens of inflammatory nonspecific vulvovaginitis. They do not interfere with the functional activity of lactobacilli, which helps maintain a normal vaginal biocenosis. The drug Geksikon with success can be recommended both for the treatment of gynecological patients of reproductive age, and pregnant women. The use of Depantol, due to the regenerative activity of the dexpanthenol component, is a priority in the treatment of infectious processes in adolescence, perimenopausal age and in patients after operations on the cervix, vagina and perineum. Key words: chlorhexidine preparations, obstetrics and gynecology, Depantol, Geksikon.

scholarly journals FEATURES OF SYSTEM IMMUNITY IN WOMEN WITH ENDOMETRIOSIS AND GENITAL INFECTION

2019 ◽  
Vol 21 (5) ◽  
pp. 895-910
Author(s):  
L. F. Zaynetdinova ◽  
L. F. Telesheva ◽  
A. V. Koryaushkina ◽  
S. V. Kvyatkovskaya ◽  
E. A. Mezenceva ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immunity ◽  
Functional Activity ◽  
Statistical Processing ◽  
Mycoplasma Genitalium ◽  
Reproductive Age ◽  
Genital Infection ◽  
Blood Monocytes ◽  
Women Of Reproductive Age ◽  
Systemic Immunity

External genital endometriosis is an inflammatory, estrogen-dependent disease that develops predominantly in women of reproductive age and is characterized by the presence of pain syndrome and infertility. Today, endometriosis is one of the most common gynecological diseases in women of reproductive age, however, the etiology and pathogenesis of it are not completely clear. Violations of systemic immunity are most important in the pathogenesis of endometriosis. The literature data on the features of the immune response in endometriosis in combination with genital infection are few and contradictory. Purpose – to study the features of systemic immunity in women with external genital endometriosis and pathogens of genital infection.A total of 159 women with external genital endometriosis were examined. The main lymphocyte subpopulations, the functional activity of neutrophils and peripheral blood monocytes, and the content of cytokines in the blood serum were studied. A study of systemic immunity was performed in women with 1-2 and 3-4 stages of endometriosis, as well as depending on the presence of pathogens of genital infection. The presence of Chlamydia trachomatis, Ureaplasma spp., Mycoplasma genitalium, HSV1, 2/CMV, HPV in the endometrium, peritoneal fluid, and endometrioid heterotopies was determined. Statistical processing was performed using the IBM SPSS Statistics Version 22.2 statistical analysis software package.According to the results of the study, it was found that women with endometriosis of stages 1-2 show signs of systemic inflammation with a predominance of the Th2 type of immune response and inhibition of cellular immunity. A particular feature of HPV was an increase in T-NK lymphocytes, a decrease in IL-2 and neutrophil functional activity. The presence of Ureaplasma spp./Mycoplasma genitalium was characterized by a decrease in cellular immunity and an increase in T-NK cells. Only with HPV and Ureaplasma spp./Mycoplasma genitalium decreased synthesis of IL-2, IL-6. With 3-4 stages, the most significant changes in immunity were found in groups of women with genital infection. When HPV – a high level of IgA, increased IgM, IL-8. With Ureaplasma spp./Mycoplasma genitalium – inhibition of cellular immunity, high levels of IgA, reduction of neutrophil phagocytic activity.Thus, in women with endometriosis in the presence of pathogens of genital infection revealed features that may contribute to the development and progression of the disease. 

HLA-DR expression on monocytes as a prognostic criterion for the development of pyoinflammatory processes at various stages of surgical treatment

2016 ◽  
Vol 94 (5) ◽  
pp. 379-382
Author(s):  
Mariya V. Chepeleva
Keyword(s):  
Monoclonal Antibodies ◽  
Surgical Treatment ◽  
Periprosthetic Infection ◽  
Relative Content ◽  
Immune Disorders ◽  
Flow Cytofluorometry ◽  
Posttraumatic Osteomyelitis ◽  
Hla Dr ◽  
Prognostic Criterion ◽  
Tubular Bones

We studied relative content of CD14+HLA-DR in 214 patients including those with aseptic instability and stable implants of the knee and the hip, periprosthetic infection of the hip, chronic posttraumatic osteomyelitis of long tubular bones, and osteomyelitis generalization (sepsis). We determined HLA-DR expression on monocytes by laser flow cytofluorometry using Beckman Coulter Epics XL (USA) cytometer and monoclonal antibodies (Immunotech Company, France). It has been demonstrated, that the decrease in HLA-DR expression on monocytes can be one of the mechanisms of increasing immune disorders in patients with orthopedic pathology and monitoring HLA-DR monocytic expression can be used as a prognostic criterion of developing pyoinflammatory processes at the stages of surgical treatment.

scholarly journals Induction of Cell Death in Human Immunodeficiency Virus-Infected Macrophages and Resting Memory CD4 T Cells by TRAIL/Apo2L

2001 ◽  
Vol 75 (22) ◽  
pp. 11128-11136 ◽  
Author(s):  
Julian J. Lum ◽  
André A. Pilon ◽  
Jaime Sanchez-Dardon ◽  
Barbara N. Phenix ◽  
John E. Kim ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Leucine Zipper ◽  
Cell Types ◽  
Peripheral Blood Lymphocytes ◽  
Viral Rna ◽  
Hla Dr ◽  
Immunodeficiency Virus ◽  
P24 Antigen ◽  
Latent Hiv

ABSTRACT Because the persistence of human immunodeficiency virus (HIV) in cellular reservoirs presents an obstacle to viral eradication, we evaluated whether tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L) induces apoptosis in such reservoirs. Lymphocytes and monocyte-derived macrophages (MDM) from uninfected donors do not die following treatment with either leucine zipper human TRAIL (LZhuTRAIL) or agonistic anti-TRAIL receptor antibodies. By contrast, such treatment induces apoptosis of in vitro HIV-infected MDM as well as peripheral blood lymphocytes from HIV-infected patients, including CD4+ CD45RO+ HLA-DR− lymphocytes. In addition, LZhuTRAIL-treated cells produce less viral RNA and p24 antigen than untreated controls. Whereas untreated cultures produce large amounts of HIV RNA and p24 antigen, of seven treated CD4+ CD45RO+ HLA-DR− cell cultures, viral RNA production was undetectable in all, p24 antigen was undetectable in six, and proviral DNA was undetectable in four. These data demonstrate that TRAIL induces death of cells from HIV-infected patients, including cell types which harbor latent HIV reservoirs.

Tu1296 P-Glycoprotein 170 (P-GP) Functional Activity in Peripheral Blood Lymphocytes (PBL) According Clinical Response in IBD Patients Treated With Anti-TNFs

2015 ◽  
Vol 148 (4) ◽  
pp. S-851
Author(s):  
Alicia M. Sambuelli ◽  
Anibal H. Gil ◽  
Silvia M. Negreira ◽  
Catalina M. Cortada ◽  
Maricel I. Bellicoso ◽  
...  
Keyword(s):  
Clinical Response ◽  
Peripheral Blood ◽  
Functional Activity ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
P Glycoprotein

scholarly journals Normal uterine size in women of reproductive age in northern Islamic Republic of Iran

2004 ◽  
Vol 10 (3) ◽  
pp. 437-441
Author(s):  
S. Esmaelzadeh ◽  
N. Rezaei ◽  
M. Hajiahmadi
Keyword(s):  
Body Mass Index ◽  
Body Mass ◽  
Reproductive Age ◽  
Islamic Republic ◽  
Women Of Reproductive Age ◽  
Cross Sectional ◽  
Nulliparous Women ◽  
Multiparous Women ◽  
Islamic Republic Of Iran ◽  
Ultrasonographic Assessment

Todetermine the efficacy of ultrasonographic assessment of uterus size in women of reproductive age, we conducted a cross-sectional analytic study of 231 women aged 15-45 years in Babol, northern Islamic Republic of Iran. Mean uterus size was 86.6 mm x 49.6 mm x 40.6 mm overall, 72.8 mm x 42.8 mm x 32.4 mm for nulliparous women and 90.8 mm x 51.7 mm x 43.0 mm for multiparous women. Mean age was 31.7 +/- 9.6 years and mean body mass index [BMI] was 24.7 +/- 4.0 kg/m2. Uterus size was significantly associated with parity and age; but not with BMI. Our findings show a greater mean uterus size than reported by others. Ultrasonographic measurement of uterus size is valuable for predicting pathologies associated with abnormal uterine size

FEATURES OF THE FUNCTIONAL STATE OF PLATELETS AT THE PRE-CLINICAL STAGE OF PREECLAMPSIA

2020 ◽  
pp. 39-43
Author(s):  
Grigorieva N.A. ◽  
Glukhova T.N.
Keyword(s):  
Pregnant Women ◽  
Varicose Veins ◽  
Heart Defects ◽  
Main Group ◽  
The State ◽  
Reproductive Age ◽  
Average Volume ◽  
Hemostasis System ◽  
Stage Of Development ◽  
Autoimmune Pathology

The only effective method of treating pregnant women with developed preeclampsia is timely delivery, so it is an urgent task to establish predictors of preeclampsia in order to predict its development.The aim of the work is to evaluate the platelet link of the hemostasis system in the 2nd trimester of pregnancy in patients with preeclampsia that developed after 34 weeks of pregnancy, to establish informative markers for predicting the development of preeclampsia after 34 weeks of pregnancy A survey of 210 apparently healthy women of active reproductive age (18-34 years) was carried out. Patients with multiple pregnancies, autoimmune pathology, arterial hypertension, heart defects, varicose veins, diabetes mellitus, hereditary thrombophilia were excluded from the study. All patients received voluntary informed consent to use the survey data for scientific purposes. In 20 patients, the course of pregnancy was complicated by the development of moderate preeclampsia after 34 weeks of pregnancy, these patients constituted the main group. 190 women with physiological pregnancy made up the comparison group. The study of the state of the platelet link of the hemostasis system was carried out using a VS-3000 + hematological analyzer, the number of platelets, their average volume, the root-mean-square value of the spread of platelet sizes, and the percentage of megalothrombocytes were determined. At the preclinical stage of development of preeclampsia (at 20-22 weeks), a decrease in the number of platelets and an increase in functional activity in the form of an increase in the rate of platelet anisocytosis, the average volume of platelets and the number of megalothrombocytes were found in pregnant women of the main group. Determination of indicators of the state of the platelet hemostasis at 20-22 weeks of gestation can be used to predict the development of moderate preeclampsia after 34 weeks of gestation.

Engraftment of human peripheral blood lymphocytes in normal strains of mice

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2368-2381 ◽  
Author(s):  
I Lubin ◽  
H Segall ◽  
H Marcus ◽  
M David ◽  
L Kulova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
Peripheral Blood ◽  
Scid Mouse ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Internal Organs ◽  
Human Peripheral Blood Lymphocytes ◽  
Hla Dr ◽  
Human Pbls

Transplantation of bone marrow from SCID mice into lethally irradiated normal mice can potentially endow the normal recipients with characteristics typical of the immune-deficient SCID mouse. In the present study, we investigated whether intraperitoneal grafting of human peripheral blood lymphocytes (PBLs), which has been documented in the SCID mouse, can also be achieved in irradiated BALB/c mice radioprotected with SCID bone marrow. Evaluation of different radiation protocols suggested that, considering the quality of engraftment and rate of survival, optimal results were obtained with split dose total body irradiation (TBI; 4 Gy followed 3 days later by 10 Gy). Monitoring of mouse T cells in peripheral blood indicated an inverse correlation between the presence of such cells and the engraftment of human CD45+ cells in the peritoneum. Also, engraftment of human PBLs in nude BALB/c mice, conditioned with the same radiation protocol, was significantly higher than that achieved in their normal counterparts. Further improvement of human PBL engraftment was found when the mice were thymectomized 2 weeks before conditioning with split TBI. After transplantation of 80 x 10(6) human PBLs in such recipients, a marked engraftment of human T cells and B cells in the peritoneum cavity could be detected for at least 2 months, whereas significant amounts of human Ig could be detected for more than 3 months. Migration of human PBLs into internal organs such as spleen, liver, kidney, and lungs (and into thymus in nonthymectomized mice) was found within a few days of grafting and also persisted for 2 to 3 months. The majority of the engrafted lymphocytes were single-positive CD4+ and CD8+ T lymphocytes, about 50% of which were activated, as judged by their expression of HLA- DR. Staining with anti-CD25 antibody was lower compared with that found with anti-HLA-DR. CD20+ B cells were detected in all of the above- mentioned internal organs, but were mainly concentrated in the spleen. CD14+ monocytes could be detected only during the first week posttransplant of PBLs. Total human Ig in peripheral blood reached an average of 2.8 mg/mL 14 days posttransplant, and continued to be significant for several months. In vitro transformation by Epstein-Barr virus of human B cells from different tissues could be established 30 days after transplantation and led to outgrowth of two IgG+ cell lines, two IgM+ cell lines, and one IgA+ cell line producing 0.6 to 4.2 micrograms/mL human Ig in the supernatant.

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