scholarly journals REPELLENCY OF CELERY ESSENTIAL OILS (Apium graveolens L.) AGAINST Spodoptera frugiperda (Lepidoptera: Noctuidae) IN THE LABORATORY

2021 ◽  
Vol 21 (2) ◽  
pp. 91-96
Author(s):  
Trisnani Alif ◽  
Fita Fitriatul Wahidah

Repellency of celery essential oil (Apium graveolens L.) against Spodoptera frugiperda (Lepidoptera: Noctuidae) in thelaboratory. Essential oil is one of the ingredients that has repellent ability to herbivorous insects. Celery is one of plantscontaining essential oil which may also have the repellent ability. This study was aimed to determine the differences in therepellent ability of celery essential oils compounds that were taken from 3 different locations against S. frugiperda in thelaboratory. This research was arranged in a factorial completely randomized design (CRD) with two factors. The first factorwas the origin of the celery plants (Malang, Surabaya, and Lamongan) and the second factor was the concentration level ofessential oils (1000, 2000, and 3000 ppm) which was repeated three times. The reliability test was carried out using filter papermethod. The results showed that the essential oil extracted from celery originated from Malang with 3000 ppm concentrationhad the highest percentage level of repellency (level 5) that reached 93.33% of repellency. Location and concentration factorshad no different effect on S. frugiperda larvae repellency. There was no correlation between location and concentration on therepellency of S. frugiperda larvae.

Author(s):  
Andrew Setiawan Rusdianto ◽  
Andi Eko Wiyono ◽  
Felly Halsa Fiana

Aromatherapy is a therapy that utilizes steam from the essential oils of certain plants. Essential oils are oils produced from plant parts, such as roots, bark, stems, flowers, leaves, and seeds that have volatile properties at room temperature without undergoing decomposition by means of distillation. This study aims to determine the effect of a comparison of the concentration level of essential oils on physical properties and consumer preferences and to find out the aromatherapy massage oil formulation that produces the best massage oil. This study uses a completely randomized design (CRD) with 1 factor, namely the difference in the ratio of the essential oil of the nightly flower to the essential oil of lime. The experiment was carried out 2 times. The usual dilution was 1 ml of essential oil in 50 ml of carrier oil. The treatments are P1 (0.2 ml of nightly essential oil: 0.8 ml of lime essential oil), P2 (0.4 ml of nightly essential oil: 0.6 ml of lime essential oil), P3 (0.5 ml nightly essential oils: 0.5 ml lime essential oil, P4 (0.6 ml nightly essential oils: 0.4 ml lime essential oil), and P5 (0.8 ml nightly essential oils), 2 ml of lime essential oil). The parameters observed were pH, specific gravity, viscosity, refractive index, color, and hedonic test.


2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Juliana Dantas de Oliveira ◽  
Daniely Karen Matias Alves ◽  
Mayker Lazaro Dantas Miranda ◽  
José Milton Alves ◽  
Marcelo Nogueira Xavier ◽  
...  

ABSTRACT: Campomanesia adamantium is a native fruit species of the Cerrado and is used in food and medicines and as bee pasture. The chemical composition of essential oils obtained from plants of the same species have varying constituent proportions due to the influence of extractive factors, environmental, genetic and ontogenetic. This study aimed to identify the influence of hydrodistillation time on the content and chemical composition of essential oil extracted from the leaves of C. adamantium . Treatments consisted of five extraction times (1, 2, 3, 4, and 5h) using Clevenger with five replications in a completely randomized design. It was observed that after two hours of hydrodistillation, the essential oil content remains constant. Regarding the chemical constituents of essential oil, variation of the proportions of the compounds tested occurred at all hydrodistillation times. The compounds spathulenol oxygenated sesquiterpenes and caryophyllene oxide were the majority in the five hydrodistillation times.


2016 ◽  
Vol 29 (3) ◽  
pp. 642-647 ◽  
Author(s):  
ANA CLÁUDIA VIEIRA DOS SANTOS ◽  
CARROMBERTH CARIOCA FERNANDES ◽  
LUCAS MARTINS LOPES ◽  
ADALBERTO HIPÓLITO DE SOUSA

ABSTRACT The potential insecticidal of oils from southwestern Amazon plants against Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) was investigated. Initial bioassays were performed with undiluted oils from 11 plant species. The efficacy of the oils was evaluated against eggs and third -instar caterpillars of S. frugiperda. The oils of Copaifera sp. (Leguminosae), Orbignya phalerata (Arecaceae), and Carapa guianensis (Meliaceae) displayed a high efficacy against the caterpillars and were used in subsequent concentration-response bioassays, at concentrations established through preliminary tests. The highest nonlethal concentrations of oils and the lowest lethal concentrations were calculated. A completely randomized design was adopted in both bioassays. The LC50 of the oils varied from 7.50 to 60.84% (v/v). Copaifera sp. oil had the highest toxicity and was 6.84-fold more toxic than O. phalerata oil and 8.11-fold more toxic than Carapa guianensis oil. In general, oils from Copaifera sp., O. phalerata, and Carapa guianensis were effective in controlling S. frugiperda caterpillars under laboratory conditions, and are good candidates for use in integrated management programs of corn pests.


2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Juliana Oliveira de Melo ◽  
Arie Fitzgerald Blank ◽  
Alisson Marcel Souza de Oliveira ◽  
Thiago Matos Andrade ◽  
Maria de Fátima Arrigoni-Blank ◽  
...  

Lippia gracilis, popularly known in Brazil as ‘alecrim-de-tabuleiro’, is used for many purposes, especially as antimicrobial and antiseptic. The drying process of aromatic and medicinal plants aims to minimize the loss of active principles and slow their deterioration, which may greatly influence the yield and chemical composition of some species. The objective of this study was to evaluate the effect of drying times (0, 2, 4, and 8 days) on the content and chemical composition of the essential oil of L. gracilis accessions LGRA-106, LGRA-109, and LGRA-201. The leaves were dried at 40 oC, and essential oil was extracted by hydrodistillation. Chemical analysis was performed by GC/MS. The experiment was carried out in a completely randomized design with three replications. The accessions of L. gracilis LGRA-106, LGRA-109, and LGRA-201 presented higher essential oil at four days of drying time. The accession LGRA-201 showed the highest essential oil yields at four and eight days of drying, with mean values of 0.038 and 0.029 mL g-1, respectively. The drying time did not influence the contents of thymol, methyl-thymol, γ-terpinene, and carvacrol in the essential oils of L. gracilis, but affected the contents of β-caryophyllene, p-cymene, and carvacrol acetate. The essential oils of the three accessions analyzed in this study revealed different chemical profiles.


2017 ◽  
Vol 19 (2) ◽  
pp. 39
Author(s):  
Supyani Supyani ◽  
Salim Widono ◽  
Ulfaizah Ulfaizah

<p>Production of cowpea has declined, one of them caused by virus infection that caused mosaic disease which was still difficult to controlled. This study aimed to determine the effectivity of antiviral compounds on lemongrass essential oils to development of mosaic disease and the relationship to growth and development of cowpea that infected. This study used Completely Randomized Design (CRD) with two factors, they are the time of application and concentration of lemongrass essential oil. Data were analyzed using F test and DMRT at level of 5%. Results showed that application of lemongrass essential oil at concentration of 1,8% before planting (soaking seeds) tend to delaying the emergence of mosaic disease symptoms. Application of lemongrass essential oil at all of concentration that used, can inhibiting the disease intensity up to 31.39%. However, apllication of lemongrass essential oils in plants infected with a virus that causes mosaic has not able to improve plant growth and development.</p>


2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 2933
Author(s):  
Ana Paula de Queiroz ◽  
Bruna Magda Favetti ◽  
Pamela Gislaine Luski ◽  
Jaciara Gonçalves ◽  
Pedro Manuel Oliveira Janeiro Neves ◽  
...  

Abstract: Release of egg parasitoids for biological control of pests is a promising technique in integrated pest management (IPM). However, there is a lack of information on the performance of parasitoid females of different ages, and specifically on the behavior of the parasitoid Telenomus remus towards pest eggs at different stages of embryonic development. Thus, the relationships between host age, parasitoid age, and parasitism by T. remus on Spodoptera frugiperda eggs were evaluated. Three separate bioassays were performed, each in a completely randomized design. In the first bioassay, T. remus females grouped by age in days (ranging from 1 to 10 days old) were offered 100 ± 20 eggs of S. frugiperda for 24 hours. In the second bioassay, 100 ± 20 eggs of S. frugiperda (24, 48 or 72 hours old) were offered to females of T. remus for 24 hours. In the third bioassay, 24, 48- and 72-hour-old host eggs of S. frugiperda were offered to T. remus females in a choice test. The variables evaluated were: number of parasitized eggs, parasitoid emergence (%), and sex ratio of progeny in bioassays 1 and 2, and the number of eggs parasitized in bioassay 3. The age of T. remus females did not affect the number of S. frugiperda eggs parasitized or emergence of the progeny. However, the sex ratio was more male-biased in the progeny of 1- and 2-day-old females compared to older wasps. In bioassay 2, the highest parasitism was observed in 24- and 48-hour-old eggs. Percentage emergence and sex ratios were not influenced by the ages of the eggs tested. Telenomus remus preferred to parasitize 24-hour-old eggs in bioassays 3. Overall, the age of T. remus females tested did not affect the parasitism of S. frugiperda eggs, but the number of eggs parasitized decreased with increasing host age.


Author(s):  
Alice M. N. de Araújo ◽  
José V. de Oliveira ◽  
Solange M. França ◽  
Daniela M. do A. F. Navarro ◽  
Douglas R. e S. Barbosa ◽  
...  

ABSTRACT This study had the following objectives: to identify and quantify the constituent compounds of essential oils from Cymbopogon winterianus Jowitt, Eucalyptus globulus Labill., Eucalyptus staigeriana F. Muell. ex F.M. Bailey, Foeniculum vulgare Mill., Ocimum basilicum L., Ocimum gratissimum L., and Piper hispidinervum C. DC., investigate their toxicity and repellency to S. zeamais and evaluate the toxicity of P. hispidinervum to immature S. zeamais. Individual tests for each essential oil were conducted with a completely randomized design with five concentrations for each oil and four replicates. Gas chromatography-mass spectrometry revealed the presence of citronellal in C. winterianus, 1,8-cineole in E. globulus, limonene in E. staigeriana, limonene in F. vulgare, linalool in O. basilicum, (E)-anethole in O. gratissimum, and safrole in P. hispidinervum. The median lethal concentration required to kill 50% of the insect (LC50) in contact and ingestion toxicity tests ranged from 5.12 to 78.89 μL 40g-1 corn in P. hispidinervum and C. winterianus, respectively. In adult fumigation tests, the LC50 ranged from 2.1 to 19.4 µL L-1 air, and in immature fumigation tests, the egg stage was susceptible to essential oil, whereas larval and pupal phases were tolerant. All of the oils repelled S. zeamais.


2016 ◽  
Vol 46 (5) ◽  
pp. 764-770 ◽  
Author(s):  
Cristina Batista de Lima ◽  
Luana Lopes Assumpção Rentschler ◽  
João Tavares Bueno ◽  
Ana Cláudia Boaventura

ABSTRACT: The present study aims to investigate garlic, pepper and coriander plant extract as well as neem and orange peel essential oil effective ness to control Alternaria alternata and Alternaria dauci and their efficiency during carrot seeds germination and emergence. A completely randomized design was used in three different experiments. The first experiment evaluated the effect of plant extracts and essential oils on the incidence of A. alternata and A. dauci . It was done by means of a factorial design applied to five treatments (garlic, pepper, coriander, and neem and orange peel essential oils) at three concentrations (10, 20 and 30%).They also evaluated the controls untreated and with fungicide (Thiram).The second experiment evaluated the effect of the treatment at30% concentration on the germination and emergence of seedlings assessed on trays, and in the third experiment, the presence of A. alternata and A. dauci on the pericarp, endosperm and on the embryo. A. alternata showed higher incidence than A. dauci. The garlic extract and the orange essential oil showed the potential to control A. dauci and A. alternata, because their lower concentrations were able to sufficiently reduce the incidence of these fungi and because they do not affect carrot seeds germination and emergence. A. alternata conidia were found on the embryo (8%), pericarp (17%) and endosperm (31%).


2019 ◽  
Vol 112 (5) ◽  
pp. 2167-2176 ◽  
Author(s):  
Torranis Ruttanaphan ◽  
Wanchai Pluempanupat ◽  
Chutikan Aungsirisawat ◽  
Polnarong Boonyarit ◽  
Gaelle Le Goff ◽  
...  

Abstract Essential oils are well known to act as biopesticides. This research evaluated the acute toxicity and synergistic effect of essential oil compounds in combination with cypermethrin against Spodoptera litura Fabricius (Lepidoptera: Noctuidae). The effects of distillation extracts of essential oils from Alpinia galanga Zingiberaceae (Zingiberales) rhizomes and Ocimum basilicum Lamiaceae (Lamiales) leaves; one of their primary essential oil compounds 1,8-cineole; and linalool were studied on second-instar S. litura by topical application under laboratory conditions. The results showed that A. galanga had the highest control efficiency, whereas1,8-cineole provided a moderate efficacy. The mixtures of linalool, 1,8-cineole, O. basilicum, or A. galanga with cypermethrin were synergistic on mortality. Activity measurements of the main detoxification enzymes show that linalool and 1,8-cineole inhibit the activity of cytochromes P450 and carboxylesterases, which could explain their synergistic effect. Based on our results, the use of these mixtures represents an ideal eco-friendly approach, helping to manage cypermethrin resistance of S. litura.


2020 ◽  
Vol 80 (4) ◽  
pp. 617-628
Author(s):  
Kássio E.S. Sombra ◽  
Caio V.S. de Aguiar ◽  
Sabrina J. de Oliveira ◽  
Marianne G. Barbosa ◽  
Guilherme J. Zocolo ◽  
...  

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