scholarly journals Mitigating Effect of Vitamin-E on Copper Sulphate-Induced Toxicity in African Catfish (Clarias gariepinus)

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
O. I. Azeez ◽  
S. F. Braimah
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Copper Sulphate ◽  
Biochemical Parameters ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Fish Feed ◽  
Antioxidant Defence System ◽  
Liver And Kidney ◽  
Radical Generation

Copper sulphate is widely used not only in livestock production especially in the treatment of foot rot in small ruminants but also in aquaculture as algaecide and an ectoparasiticide in hatcheries. Meanwhile, it is a common environmental contaminant of water bodies, with carcinogenic, mutagenic and teratogenic effects in humans and animals. The present study was therefore designed to evaluate the toxic effects of copper sulphate and the protective activities of vitamin E on haematological and biochemical parameters as well as oxidative stress status in the African catfish (Clarias gariepinus) Sixty juvenile African catfish with an average weight of 120g were used for the study. They were assigned into six groups (A-F) consisting of ten fish per group. Group A served as the control group and was fed with normal pelletized fish feed only, group B was fed with Vitamin E-supplemented feed only, groups C and D were exposed to copper sulphate (20mg/L and 5mg/L) respectively, plus normal pelletized fish feed while groups E and F were exposed to copper sulphate (20mg/L and 5mg/L), respectively, plus Vitamin E-supplemented feed (240mg/kg feed). Blood samples were collected for haematology and plasma biochemical parameters while gills, liver and kidney samples were collected for evaluation markers of oxidative stress. Exposure to copper sulphate led to a significant decrease in PCV, RBC, Hb concentration, MCV, MCH and total WBC when compared with the unexposed control and those fed with vitamin E-supplemented feed. Furthermore, exposure to copper sulphate caused liver and kidney damages and cell impairment by increasing plasma ALT, AST and ALP activities. It also led to increased oxidative stress as the concentrations of antioxidant endogenous enzymes - GPx, GST and GSH were depleted while potentiating lipid peroxidation and hydroxyl radical generation. The changes in the haematological, biochemical and antioxidant parameters were restored in the fish fed with vitamin E-supplemented feed. In conclusion, the study showed that exposure to copper sulphate is toxic to African catfish, causing anaemia and liver damage through free radical generation and depletion of antioxidant defence system. Vitamin – E supplementation is therefore recommended during the use of CuSO4 in aquaculture. Release of the compound to the environment must however be avoided at all cost.

scholarly journals Protective Effect of Vitamin E on Potassium Dichromate-induced Haematoxicity and Oxidative Stress in African Catfish (Clarias gariepinus)

2020 ◽  
pp. 18-31
Author(s):  
O. I. Azeez ◽  
S. F. Braimah
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Potassium Dichromate ◽  
Clarias Gariepinus ◽  
Haemoglobin Concentration ◽  
African Catfish ◽  
Antioxidant Defence System ◽  
Antioxidant Parameters ◽  
Radical Generation ◽  
And Oxidative Stress

Effect of potassium dichromate was studied experimentally in African Catfish (Clarias gariepinus) with and without vitamin E in induced haematoxicity and oxidative stress. Blood samples were collected for haematology and plasma biochemical parameters while gills, liver and kidney samples were collected for evaluation of markers of oxidative stress. Exposure to potassium dichromate led to a significant decrease in PCV, RBC, haemoglobin concentration, MCV, MCH and total WBC when compared with the unexposed     control and those fed with vitamin E-supplemented feed. Nephrotoxicity was also observed as evidenced by increases in plasma creatinine levels. Exposure to potassium dichromate also led to increased oxidative stress as the concentrations of antioxidant endogenous enzymes - GPx, GST and GSH were depleted while potentiating lipid peroxidation and hydroxyl radical generation. The changes in the haematological, biochemical and antioxidant parameters were restored in the fish fed with vitamin E-supplemented feed. The study showed exposure to potassium dichromate to be toxic to African catfish, causing anaemia and kidney damage with free radical generation and depletion of GSH and other antioxidant defence system but the toxic effect can be minimised along with weight gain in fish supplemented by vitamin – E in aquaculture.

Vitamin E as antioxidant in female african catfish (Clarias gariepinus) exposed to chronic toxicity of atrazine

2012 ◽  
Vol 16 (2) ◽  
pp. 83-98 ◽  
Author(s):  
Shadia Kadry ◽  
Afnan Amer ◽  
Mohamed Marzouk ◽  
Magdy Hanna ◽  
Amal Azmy ◽  
...  
Keyword(s):  
Vitamin E ◽  
Chronic Toxicity ◽  
Clarias Gariepinus ◽  
African Catfish

Eudragit L-100 Capsules Aromatize and Quaternerize Chitosan for Insulin Nanoparticle Oral Delivery During Toxic Oxidative Stress in Rat Liver and Kidney

2020 ◽  
Vol 8 (3) ◽  
pp. 239-254 ◽  
Author(s):  
Reza Mahjub ◽  
Farzane K. Najafabadi ◽  
Narges Dehkhodaei ◽  
Nejat Kheiripour ◽  
Amir N. Ahmadabadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oral Administration ◽  
Oral Delivery ◽  
Biochemical Parameters ◽  
Kidney Injury ◽  
Regular Insulin ◽  
Treated Group ◽  
Histopathological Change ◽  
Diabetic Rats ◽  
Liver And Kidney

Background: Insulin, like most peptides, is classified as a hydrophilic and macromolecular drug that is considered as a low permeable and unstable compound in the gastrointestinal (GI) tract. The acidic condition of the stomach can degrade insulin molecules. Moreover, the presence of proteolytic activities of some enzymes such as trypsin and chymotrypsin can hydrolyze amide-bonds between various amino-acids in the structures of peptides and proteins. However, due to its simplicity and high patient compliance, oral administration is the most preferred route of systemic drug delivery, and for the development of an oral delivery system, some obstacles in oral administration of peptides and proteins including low permeability and low stability of the proteins in GI should be overcome. Objective: In this study, the effects of orally insulin nanoparticles (INPs) prepared from quaternerized N-aryl derivatives of chitosan on the biochemical factors of the liver in diabetic rats were studied. Methods: INPs composed of methylated (amino benzyl) chitosan were prepared by the PEC method. Lyophilized INPs were filled in pre-clinical capsules, and the capsules were enteric-coated with Eudragit L100. Twenty Male Wistar rats were randomly divided into four groups: group1: normal control rats, group 2: diabetic rats, group 3: diabetic rats received capsules INPs(30 U/kg/day, orally), group 4: the diabetic rats received regular insulin (5 U/kg/day, subcutaneously). At the end of the treatment, serum, liver and kidney tissues were collected. Biochemical parameters in serum were measured using spectrophotometric methods. Also, oxidative stress was measured in plasma, liver and kidney. Histological studies were performed using H and E staining . Results: Biochemical parameters, and liver and kidney injury markers in serum of the diabetic rats that received INPs improved significantly compared with the diabetic group. INPs reduced oxidative toxic stress biomarkers in serum, liver and kidney of the diabetic treated group. Furthermore, a histopathological change was developed in the treated groups. Conclusion: Capsulated INPs can prevent diabetic liver and oxidative kidney damages (similar regular insulin). Therefore oral administration of INPs appears to be safe. Lay Summary: Although oral route is the most preferred route of administration, but oral delivery of peptides and proteins is still a challenging issue. Diabetes Mellitus may lead to severe complications, which most of them are life-threatening. In this study, we are testing the toxicity of oral insulin nanoparticles in kidney and liver of rats. For this investigation, we will prepare insulin nanoparticles composed of a quaternized derivative of chitosan. The nanoparticles will be administered orally to rats and the level of oxidative stress in their liver and kidney will be determined. The data will be compared to the subcutaneous injection of insulin.

scholarly journals Effect of Humic Substances as Feed Additive on the Growth Performance, Antioxidant Status, and Health Condition of African Catfish (Clarias gariepinus, Burchell 1822)

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2266
Author(s):  
Markéta Prokešová ◽  
Milena Bušová ◽  
Mahyar Zare ◽  
Hung Quang Tran ◽  
Eliška Kučerová ◽  
...  
Keyword(s):  
Growth Performance ◽  
Humic Substances ◽  
Biochemical Parameters ◽  
Antioxidant Status ◽  
Clarias Gariepinus ◽  
Feed Additive ◽  
African Catfish ◽  
Growth Promoter ◽  
Total N ◽  
Positive Effects

In the present study, a possible nature immunostimulant and growth promoter—humic substances (HS) originating from Siberian leonardite mineraloid—were tested on juvenile Clarias gariepinus performance. Feed additive was applied onto commercial pelleted feed at four HS levels—0, 1, 3, and 6% w/w (HS0, HS1, HS3, HS6, respectively). Diets were tested in five repetitions (in total, n = 1800 individuals, mean body weight 28.1 ± 6.2 g) for 56 days. Growth and production parameters, fish condition and somatic indices, and overall mortality were evaluated after 14, 28, 42, and 56 days of exposure. Whereas, plasma samples were collected only after 0, 28, and 56 days, when fish health status was assessed with biochemical parameters (total proteins, TP; alanine aminotransferase, ALT; aspartate aminotransferase, AST; lactate dehydrogenase, LDH; cholesterol, CHOL; triglycerides, TAG) and fish antioxidant status with glutathione (reduced glutathione, GSH; oxidized glutathione, GSSG; glutathione ratio GSH/GSSG). Although a significantly positive effect of HS feed additive on growth performance was not found in the present study, moderately positive effects were found regarding biochemical parameters (ALT, AST, LDH, CHOL, and TAG) and antioxidants (GSH/GSSG ratio) that were improved especially in the HS3 group.

scholarly journals TEPUNG DAUN SINGKONG (Monihot utilissima) TUA SEBAGAI SUMBER PROTEIN ALTERNATIF DALAM FORMULA PAKAN IKAN LELE (Clarias gariepinus)

2016 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Syahrizal Syahrizal ◽  
Muarofah Ghofur ◽  
. Safratilofa ◽  
Rahmat Sam
Keyword(s):  
Survival Rate ◽  
Fish Meal ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Fish Feed ◽  
Daily Growth ◽  
Protein Substitute ◽  
Feed Formulation ◽  
Cassava Leaves ◽  
Alternative Protein

AbstractThe feed as a source of energy for the growth of fish is a component of the most important costs 40-89% and the quality should be good. The solution is through research. Research in the form of meal cassava leaves (Monihot utilissima) parents as a source of alternative protein substitute for fish meal in feed formulation catfish (Clarias gariepinus). The design used Complete Random Design with 4 treatments and 3 repetitions. The results showed that for the growth and the survival between treatments were not significant (P <0.5), meaning that all treatments were no differences can be categorized and feed ingredients of flour cassava leaves can replace most of the presence of meal fish  in fish feed formulas African catfish. Growth of the best catfish are on treatment A (55% meal cassava leaf: 00% fish meal) with daily growth of 8.27 grams was 2.61% and the B (40%% meal cassava leaves: 15% meal fish) 5.28 gram with daily growth is 1.86%, followed by C (15%%  meal cassava leaves: 15% meal fish ) 1:51% and D (0% meal cassava leaves : 55% meal fish ) 1:33%. Catfish survival rate was not significant (P <0.5), and relatively equally well A (96.17%), B (94.77) and C (95.92) and the best in treatment for D (96.37 ). As users are advised to wear formulations in treatment B (40% meal fish and 15%  meal cassava leaves old). Keywords: Catfish, Ffeed, Meal fish, Meal cassava leaves AbstrakPakan sebagai sumber energi bagi pertumbuhan ikan  merupakan komponen biaya yang paling besar 40-89%  dan kualitasnya harus baik. Solusinya melalui penelitian. Penelitian berupa tepung daun  singkong (Monihot utilissima) tua sebagai sumber protein alternatif penganti tepung ikan dalam formulasi pakan ikan lele (Clarias gariepinus). Rancangan digunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 3 kali ulangan. Hasil penelitian menunjukan bahwa untuk pertumbuhan dan kelulusan hidup antar perlakuan tidak signifikan (P < 0,5), artinya semua perlakuan  tidak ada perbedaan dan dapat dikatagorikan bahan pakan dari tepung daun singkong dapat mengantikan sebagian keberadaan tepung ikan dalam formula pakan ikan lele dumbo. Pertumbuhan ikan lele terbaik terdapat pada perlakuan A (55% tepung daun singkong : 00% tepung ikan) 8,27 gram dengan pertumbuhan harian adalah 2.61% dan  pada B (40%  % tepung daun singkong : 15% tepung ikan) 5,28 gram dengan pertumbuhan harian adalah 1.86%, diikuti C (15% % tepung daun singkong :15% tepung ikan) 1.51% dan D (0 % tepung daun singkong : 55% tepung ikan) 1.33%. Tingkat kelangsungan hidup ikan lele tidak signifikan (P < 0,5), dan  relatif sama baiknya A (96,17%), B (94,77) dan C (95,92) dan terbaik pada perlakuan untuk D (96,37). Sebagai pengguna disarankan memakai formulasi pada perlakuan B (40% Tepung ikan dan 15% tepung daun singkong tua). Kata kunci: Ikan lele, Pakan, Tepung ikan, Tepung  Daun Singkong Tua

scholarly journals Hepatic Histomorphological Changes and Oxidative Stress Profile of Clarias gariepinus (Burchell, 1822) Juveniles Fed with Sodium Propanoate-Preserved Diets

2018 ◽  
Vol 10 (4) ◽  
pp. 460-465
Author(s):  
Patrick E. ABA ◽  
Ifeanyi E. UZOCHUKWU ◽  
Nelson I. OSSAI ◽  
Ifeanyi G. EKE
Keyword(s):  
Oxidative Stress ◽  
Biological Effects ◽  
Clarias Gariepinus ◽  
Basal Diet ◽  
Serum Levels ◽  
Control Group ◽  
Stress Profile ◽  
Fish Feed ◽  
Inflammatory Parameters ◽  
Significant Difference

Sodium propanoate is in the list of approved feed preservatives. However, there is dearth of information on its biological effects on the C. gariepinus. The present study investigated the effect of sodium propanoate-preserved feed on the hepatic histomorphometric changes, oxidative stress and inflammatory parameters of C. gariepinus juveniles. One hundred juveniles of mixed sexes, assigned into 5 groups of 20 fish per group, with each group consisting of 2 replicates of 10 fish, were used for the investigation. Group A juveniles were fed basal diet, while groups B-E received basal diet incorporated with sodium propanoate at the rate of 25, 50, 75 and 100 g/15 kg of feed respectively. Treatments were done two times daily for 8 weeks. Samples (sera and liver) were collected on the last day for evaluation of a few biochemical parameters (malondialdehyde values, catalase activity, C-reactive protein levels) and histomorphometric alterations in the liver. Results indicated that fish in groups D and E had higher catalase activities, lower serum levels of C-reactive proteins and an intact hepatic histomorphormetry when compared with the control group. There was no significant difference in the plasma malondialdehyde values in all the groups. It was concluded that preservation of fish feed with sodium propanoates improved antioxidant status of C. gariepinus and protected liver histology.

Negative impacts of ultraviolet-A radiation on antioxidant and oxidative stress biomarkers of African catfish Clarias gariepinus

2015 ◽  
Vol 14 (7) ◽  
pp. 1337-1345 ◽  
Author(s):  
Ahmed Th. A. Ibrahim
Keyword(s):  
Oxidative Stress ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Aquatic Species ◽  
Ultraviolet A ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Negative Impacts ◽  
And Oxidative Stress

The present study was carried out to evaluate the ultraviolet-A (UVA) effects on biochemical, oxidative stress and antioxidant changes using aquatic species.

scholarly journals GROWTH OF AFRICAN CATFISH (Clarias gariepinus) JUVENILES FED GRADED LEVELS OF ROASTED RICE CHAFF AS ENERGY SOURCE

2020 ◽  
Vol 4 (2) ◽  
pp. 386-391
Author(s):  
Ahmed Salihu Dan-kishiya ◽  
C. C. Ekpenwa
Keyword(s):  
Energy Source ◽  
Body Weight ◽  
Low Cost ◽  
Block Design ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Fish Feed ◽  
Feed Conversion ◽  
Crude Fibre ◽  
Crude Lipid

The high cost of Maize as energy source in fish feed has necessitated the need to search for low cost and locally available indigenous sources. A six months feeding trial was conducted to examine the effects of replacing maize with roasted rice chaff as a dietary energy source on the growth of juveniles of Clarias gariepinus using Completely Randomized block Design with five different treatments. The fishes were subjected to five different inclusion levels of roasted rice chaff meal (0%, 25%, 50%, 75% and 100%). The trial was conducted in a 50litres plastic bowl with replicates and feeding was done initially at 3% body weight, but the quantity of feeds was adjusted based on the new weight gain. The measurements of the fishes were carried out biweekly according to standard methods and procedures. The Roasted rice chaff meal (RRCM) contains 5.93% crude protein, 14.53% crude fibre, 0.92% moisture content, while Ash content, crude lipid and carbohydrates had 19.98%, 3.28% and 55.36% respectively. Fishes fed 100% RRCM recorded the best growth performance in body weight (435.10 ± 20.60gm), total length (45.30 ± 0.70cm), Specific growth rate (1.44 ± 0.01), Feed Conversion Ratio (2.50 ± 0.04) and Feed Intake (92.00 ± 3.40). While the least growth ware observed in 0% RRCM. However, all the physico-chemical parameters measured are within the recommended range for fish culture. Therefore, the result obtained shows that roasted rice chaff can replace maize as a source of energy in Clarias gariepinus.juveniles diets without any negative effect.

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