scholarly journals Antifungal properties of sodium peroxide and sodium hypochlorite as a denture cleanser for full acrylic denture in vitro

2008 ◽  
Vol 20 (1) ◽  
Author(s):  
Gantini Subrata
Keyword(s):  
Sodium Hypochlorite ◽  
Contact Time ◽  
Optimum Concentration ◽  
Sodium Peroxide ◽  
Antifungal Effect ◽  
Low Concentration ◽  
Base Solution ◽  
Denture Cleansers ◽  
Antifungal Efficacy

Widely used materials are reported as denture cleansers are peroxide and hypochlorite. Many contradictions on the effectiveness of the commercial peroxide base solution against Candida albicans (C. albicans). Low concentration sodium hypochlorite (0.5%) is used as a household sanitizer. But it is still unknown whether it has an antifungal effect, what is the optimum concentration and contact time to destroy the yeast. The purpose of this study is to examine the antifungal efficacy of commercial peroxide-based soaking solution and low concentration sodium hypochlorite against C. albicans, to determine the optimum concentration and contact time, and at the end, to obtain an effective denture soaking solution which is safe to use, easy to get, affordable and could be used to destroy C. albicans on dentures. The research conducted was an in vitro practical test for surface disinfectant. Sixty plates of acrylic which were already incubated with C. albicans are immersed in peroxide and hypochlorite base soaking solution in different concentration and contact time. The result showed that peroxide base was not effective to C. albicans and hypochlorite base solution can destroy C. albicans in 10 minutes at a concentration of 0,125%. Thus, the use of low concentration sodium hypochlorite as a denture soaking solution can be suggested.

scholarly journals Antibacterial and Antifungal Capacity of Three Commercially Available Mouthwashes with Different Concentrations of Chlorhexidine

2021 ◽  
pp. 380-391
Author(s):  
Daniel Chavarría-Bolaños ◽  
Vicente Esparza-Villalpando ◽  
Karol Ramírez
Keyword(s):  
Escherichia Coli ◽  
Dental Plaque ◽  
Inhibitory Effect ◽  
Low Doses ◽  
Antifungal Effect ◽  
Antibacterial And Antifungal ◽  
Antiseptic Solutions ◽  
Antifungal Efficacy ◽  
Support Evidence

Chlorhexidine was introduced almost seven decades ago and has a myriad of applications in dentistry. Few studies have evaluated the antimicrobial and antifungal capacity of different concentrations of chlorhexidine mouthwashes. Therefore, the aim of this study, was to evaluate in vitro, the antibacterial and antifungal capacity of three commercially available mouthwashes in Costa Rica, with different concentrations of chlorhexidine, 0.12%, 0.06%, and 0.03%. The experimental method selected was the Kirby-Bauer method to evaluate the antibacterial and antifungal effect of each compound by measuring the inhibitory effect on Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Candida albicans strains, exposed to the antiseptic solutions. All samples showed some degree of antibacterial and antifungal effect. Even though we provide in vitro results, our findings are of relevance since all the species used in our experiment are microorganisms that may be present in dental plaque. Our results further support evidence that oral hygiene regimens may include mouthwashes with low doses of chlorhexidine and maintain reasonable antibacterial and antifungal efficacy.

scholarly journals Antifungal Effect of Silver Nitrate on Prosthodontic Dentures

2020 ◽  
Vol 24 (2) ◽  
pp. 96-101
Author(s):  
Mahmut Sertac Ozdogan ◽  
Mustafa Gumusok ◽  
Mihriban Yucel ◽  
Bedia Dinc ◽  
Muzaffer Mizrak ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Silver Nitrate ◽  
Sodium Hypochlorite ◽  
Acrylic Resin ◽  
Chlorhexidine Digluconate ◽  
Promising Alternative ◽  
Antifungal Effect ◽  
Colony Forming Unit ◽  
Colony Counting

SummaryBackground/Aim: Although there are studies about the antimicrobial activity of silver, there is no study evaluating it as a denture disinfectant. The purpose of this study was to explore the effectiveness of 6 disinfectant solutions (50% vinegar, 100% vinegar, 1% silver nitrate, 2% silver nitrate, %1 sodium hypochlorite, 0,12% chlorhexidine digluconate) in the disinfection of acrylic resin specimens contaminated in vitro by Candida albicans, as measured by residual colony-forming unit (CFU).Material and Methods: 66 pieces of 10mmx2mm acrylic resin disc samples were prepared and incubated in 1x106 cell/ml suspension of C. albicans ATCC 18804 for 24 h (one of them as a control, n=11/group). The specimens were then transferred into tubes containing 10 ml of the tested disinfectants and kept for 10 minutes in the disinfectant. After washing with saline, the specimens were vortexed to remove the microorganisms adhered to the surfaces. Colony counting of the collected microorganisms was performed on Sabouroud dextrose medium using 10−1, 10−2 and 10−3 dilutions. The results were analysed using Kruskal-Wallis and Mann-Whitney U tests (p<0,05).Results: The results showed that 1% sodium hypochlorite, %1 silver nitrate and 2% silver nitrate were the most effective against Candida Albicans (p<0,05), followed by 100% vinegar, 0,12% chlorhexidine digluconate and 50% vinegar (p<0,05).Conclusions: Within the boundaries of this study, we conclude that 1% silver nitrate is a promising alternative disinfectant to 1% sodium hypochlorite and performs better compared to 0,12 % chlorhexidine gluconate, 50% and 100% vinegar.

scholarly journals Kennel Disinfectants forMicrosporum canisandTrichophytonsp.

2015 ◽  
Vol 2015 ◽  
pp. 1-3 ◽  
Author(s):  
Karen A. Moriello
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Hypochlorite ◽  
Contact Time ◽  
Growth Control ◽  
Calcium Hypochlorite ◽  
Good Efficacy ◽  
Antifungal Efficacy ◽  
Potassium Peroxymonosulfate

The antifungal efficacy of commonly used kennel disinfectants for large surfaces was tested using naturally infective material from untreated animals (M. canisandTrichophytonsp.) soaked and macerated but unfiltered leaving visible fluorescing hairs and/or scales in the test inoculum to create a robust challenge. Disinfectants included sodium hypochlorite (1 : 32 and 1 : 100), enilconazole (1 : 100), accelerated hydrogen peroxide (1 : 16), potassium peroxymonosulfate (1% and 2%), and calcium hypochlorite “dry bleach.” Disinfectants were tested at a 1 : 10, 1 : 5, and 1 : 1 dilution of test inoculum to disinfectant with a 10 min contact time. Good efficacy was defined as a disinfectant resulting in no growth. Control plates grew >300 colonies of each pathogen per plate. Enilconazole, sodium hypochlorite (all dilutions), accelerated hydrogen peroxide, and 2% potassium peroxymonosulfate (but not 1%) inhibited all growth of both pathogens at 1 : 10, 1 : 5, and 1 : 1 dilutions. Calcium hypochlorite showed no antifungal efficacy (>300 colonies per plate). Enilconazole (1 : 100), sodium hypochlorite (1 : 32 or 1 : 100), accelerated hydrogen peroxide (1 : 16), and 2% potassium peroxymonosulfate are recommended for decontamination of kennels exposed to dermatophyte pathogens.

scholarly journals Amphotericin B Colloidal Dispersion Combined with Flucytosine with or without Fluconazole for Treatment of Murine Cryptococcal Meningitis

1998 ◽  
Vol 42 (3) ◽  
pp. 528-533 ◽  
Author(s):  
DeAnn M. Diamond ◽  
Madeline Bauer ◽  
Barbra E. Daniel ◽  
Mary Ann E. Leal ◽  
Debra Johnson ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Cryptococcal Meningitis ◽  
Sole Source ◽  
Colloidal Dispersion ◽  
Intracerebral Injection ◽  
Triple Combination ◽  
Antifungal Effect ◽  
The Brain ◽  
Antifungal Efficacy

ABSTRACT Studies with animals and in vitro studies have demonstrated that flucytosine plus amphotericin B or fluconazole has significantly improved mycologic activity against meningitis caused byCryptococcus neoformans compared to the activity of amphotericin B or fluconazole used alone. However, few doses have been tested in combination. This study evaluated the antifungal efficacy of amphotericin B colloidal dispersion (ABCD) combined with flucytosine with and without fluconazole in a murine model of cryptococcal meningitis. The following dosages were tested: ABCD at 0 to 12.5 mg/kg of body weight given intravenously 3 days/week, flucytosine at 0 to 110 mg/kg/day, and fluconazole at 0 to 50 mg/kg/day. Meningitis was established in male BALB/c mice by intracerebral injection of C. neoformans. Treatment with flucytosine with or without fluconazole dissolved in the sole source of drinking water was started on day 2; animals were sacrificed at 16 days, and the numbers of fungal colonies in the brain were quantified. A survival rate of 100% was achieved with ABCD plus flucytosine without fluconazole; however, the addition of fluconazole was required to prevent weight loss (P < 0.00001) and to achieve the maximum antifungal effect (P < 0.00001). The only region of dose combinations for which the 99% confidence intervals were less than 100 CFU/g of brain was defined by ABCD at 5.0 to 7.5 mg/kg combined with flucytosine at 20 to 60 mg/kg/day and fluconazole at 30 to 40 mg/kg/day. The triple combination of ABCD plus flucytosine and fluconazole was necessary to achieve the greatest antifungal activity.

scholarly journals In vitro virucidal activity of sodium hypochlorite against canine parvovirus type 2

2018 ◽  
Vol 146 (15) ◽  
pp. 2010-2013 ◽  
Author(s):  
A. Cavalli ◽  
M. Marinaro ◽  
C. Desario ◽  
M. Corrente ◽  
M. Camero ◽  
...  
Keyword(s):  
Organic Matter ◽  
Sodium Hypochlorite ◽  
Contact Time ◽  
Canine Parvovirus ◽  
Sodium Hypochlorite Solution ◽  
Virucidal Activity ◽  
Short Contact Time ◽  
Canine Parvovirus Type 2

AbstractCanine parvovirosis is a very contagious, severe and often lethal infectious disease of dogs caused by canine parvovirus type 2 (CPV-2). Parvoviruses are very resistant to several disinfectants while are sensitive to halogens such as sodium hypochlorite which is often used for decontamination of veterinary clinics and animal housing facilities due to its broad spectrum of activity. If compliance with vaccination programmes and with proper disinfection plans is ensured, there should be no continuous, nor frequent, CPV-2 outbreaks in kennels and veterinary clinics. However, a continuous spread of CPV-2 infections is observed, even in kennels where an appropriate vaccination programme is applied, and this imposes a re-evaluation of disinfection protocols using sodium hypochlorite. The aim of the present study was to determine the effect of concentration, contact time and presence of organic matter on the virucidal activity of sodium hypochlorite against several CPV-2 strains. A sensitive in vitro assay capable of measuring the infectivity of CPV-2 was employed to determine the efficacy of three different concentrations of sodium hypochlorite. The data indicate that using a 0.75% sodium hypochlorite solution for a short contact time (1 min) can reduce significantly the CPV-2 titres and that even lower concentrations, i.e. 0.37%, can efficiently inactivate the viruses provided that the contact time is extended to 15 min. Results also confirm the importance of cleaning before disinfection since the presence of organic matter totally abrogated the virucidal activity of sodium hypochlorite solutions against the three CPV-2 strains.

Antifungal Efficacy of Sodium Hypochlorite and Four Intracanal Medicaments: An in vitro Evaluation

2014 ◽  
Vol 4 ◽  
pp. 143-146
Author(s):  
Aravind Kudva ◽  
Harish Kumar Shetty ◽  
Sneha R Bhat ◽  
Shravan Kini
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Evaluation ◽  
Antifungal Efficacy

Investigation of in vitro antifungal efficacy of selected extracts of Greek natural products on Malassezia sps

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
A Velegraki ◽  
K Graikou ◽  
S Kritikou ◽  
M Varsani ◽  
I Chinou
Keyword(s):  
Natural Products ◽  
Antifungal Efficacy

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

The In Vitro Antibiofilm Activity of Water Leaf Extract of Papaya (Carica papaya L.) against Pseudomonas aeruginosa

2017 ◽  
Vol 2 (3) ◽  
pp. 150-163
Author(s):  
Ekajayanti Kining ◽  
Syamsul Falah ◽  
Novik Nurhidayat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contact Time ◽  
Cell Attachment ◽  
Water Extract ◽  
Opportunistic Pathogen ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Bacterial Survival ◽  
Optimum Conditions

Pseudomonas aeruginosa is one of opportunistic pathogen forming bacterial biofilm. The biofilm sustains the bacterial survival and infections. This study aimed to assess the activity of water extract of papaya leaves on inhibition of cells attachment, growth and degradation of the biofilm using crystal violet (CV) biofilm assay. Research results showed that water extract of papaya leaves contains alkaloids, tanins, flavonoids, and steroids/terpenoids and showed antibacterial activity and antibiofilm against P. aeruginosa. Addition of extract can inhibit the cell attachment and was able to degrade the biofilm of 40.92% and 48.058% respectively at optimum conditions: extract concentration of 25% (v/v), temperature 37.5 °C and contact time 45 minutes. With a concentration of 25% (v/v), temperature of 50 °C and the contact time of 3 days, extract of papaya leaves can inhibit the growth of biofilms of 39.837% v/v.

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