scholarly journals Influence of Selected Surface Disinfestants, Fungicides, and Temperature on Seed Germination and Initial Growth of Southern Seaoats (Uniola paniculata)

2005 ◽  
Vol 23 (1) ◽  
pp. 4-8 ◽  
Author(s):  
Tyler L. Burgess ◽  
Frank A. Blazich ◽  
David L. Nash ◽  
Betsy Randall-Schadel
Keyword(s):  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Seedling Growth ◽  
Seed Treatment ◽  
Initial Growth ◽  
Measured Variable ◽  
Significant Treatment ◽  
Uniola Paniculata ◽  
Total Percentage ◽  
Chlorine Bleach

Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and treated with the following selected surface disinfestants, fungicides, or combinations of these chemicals: nontreated (control), 1.3% sodium hypochlorite [NaOCl (chlorine bleach)], 2.6% sodium hypochlorite, RTU® (12.6% thiram + 0.34% thiabendazole), RTU®-PCNB (24% pentachloronitrobenzene), 1.3% sodium hypochlorite and RTU®, 2.6% sodium hypochlorite and RTU®, 1.3% sodium hypochlorite and RTU®-PCNB, or 2.6% sodium hypochlorite and RTU®-PCNB. Following treatment, seeds were germinated at an 8/16 hr thermoperiod of 35/20C (95/68F). The seed treatments and germination thermoperiod utilized were based on three preliminary trials that investigated the influence of selected surface disinfestants, fungicides, and temperature on seed germination of the species. Germination was recorded every 3 days for 30 days. Seed treatment was highly significant (P = 0.0001) for both total percentage germination and total percentage of decayed seeds. Germination of nontreated seeds was 45%, and four treatments resulted in germination > 80% [RTU®-PCNB (81%), 2.6% sodium hypochlorite and RTU® (83%), 1.3% sodium hypochlorite and RTU® (87%), and 1.3% sodium hypochlorite and RTU®-PCNB (89%)]. A subsequent experiment investigated the effects of the aforementioned treatments with the exception of 1.3% sodium hypochlorite and RTU®, both used alone, on initial seedling growth of the species. Following treatment, seeds were sown in containers filled with a peat-based medium and the containers placed in a growth chamber maintained at an 8/16 hr thermoperiod of 35/20C (95/68F) with long day conditions. Emergence data were recorded every 3 days for 45 days. After 45 days, the study was terminated and additional data recorded to include plant height (height of main stem), leaf number, length and width of the two longest leaves, and top and root dry weights. Surface disinfestant, fungicide, and combination treatments were highly significant (P = 0.0004). Percentage emergence of nontreated seeds was 35% and five of the seven treatments resulted in emergence ≥ 75% [2.6% sodium hypochlorite (75%), 1.3% sodium hypochlorite and RTU® (75%), 1.3% sodium hypochlorite and RTU®-PCNB (76%), 2.6% sodium hypochlorite and RTU®-PCNB (81%), and 2.6% sodium hypochlorite and RTU® (83%)] with negligible effects on seedling growth. There were significant treatment differences regarding some of the variables used to evaluate seedling growth. In most cases these differences were due to seedlings from nontreated seeds having lower values for each measured variable than values for the same variables from treated seeds. Results of both experiments demonstrate the potential value of chemical seed treatment during production of seedling transplants of U. paniculata.

scholarly journals The effect of pre-sowing seed treatment on seedlings growth rate and their excretory activity

2020 ◽  
Vol 11 (1) ◽  
pp. 60-66
Author(s):  
A. I. Bozhkov ◽  
M. K. Kovalova ◽  
Z. A. Azeez ◽  
А. V. Goltvjansky
Keyword(s):  
Growth Rate ◽  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Seedling Growth ◽  
Root Exudates ◽  
Seed Treatment ◽  
Running Water ◽  
The Third ◽  
Pea Seeds ◽  
Sowing Seed

The importance of studying pre-sowing seed treatment lies in the possibility of regulating the rate of seed germination, the intensity of their growth and obtaining root exudates in biotechnology. The effect of three pre-sowing treatment methods was examined (control – washing with running water; the first method – washing with 0.05% sodium permanganate solution; the second method – 30 se­conds in 70% ethyl alcohol (C2H5OH) and 30 minutes in 5% sodium hypochlorite (NaOCl); the third method – 5 minutes in 70% C2H5OH and 40 minutes in 5% NaOCl) on the growth rate, germination rate, excretion rate of seeds of wheat and peas and composition (of protein, carbohydrate, amino acid content) of root exudates from the first to the third day of growth in order to obtain root exudates. It was revealed that the same pre-sowing treatment of wheat and pea seeds has a different effect on the rate and variability of seedling growth from the first to the third day, as well as on the qualitative and quantitative composition of root exudates. It was shown that pre-sowing treatment of wheat and pea seeds for 5 minutes with 70% ethanol followed by treatment with sodium hypochlorite (a “hard” treatment method) accelerates seedling growth and seed germination. This method of treatment reduces the intensity of excretion of root exudates and composition in wheat, but it increases the intensity of excretion in peas. The discovered effects can be explained by hormesis. Additionally, the third method of pre-sowing seed treatment can be used in root technologies for obtaining root exudates.

scholarly journals Germination Behavior of Rauvolfia serpentine (L.) Seeds with the use of Different Seed Treatments

2021 ◽  
Author(s):  
Amit Kumar Tiwari ◽  
Prawal P.S. Verma ◽  
Dipender Kumar ◽  
Sonveer Singh ◽  
Rakesh Kumar ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Seed Treatment ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Hot Water ◽  
Cotton Cloth ◽  
Cow Dung ◽  
Seed Treatments ◽  
Seed Propagation

Background: Sarpagandha is an indigenous medicinal herb of Indian continent. It has many medicinal properties. Due to increasing demand of Sarpagandha, its exploitation is increasing continuously but for lack of cultivation, this plant has been listed in endangered category. Commercially, it is propagated through seeds but the main barrier of seed propagation is its irregular and low germination. Hence, the current study was undertaken to study the effect of growing media and seed treatment methods on seed germination and seedling growth of Sarpagandha to eliminate the inhibitory effect of the hard stony endocarp.Methods: Seed germination and seedling growth experiments of Sarpagandha were carried out with following different seed treatments, T1 (Control), T2 (Water soaking for 24 hours), T3 (water soaking for 48 hours), T4 (Water soaking for 24 hours + cotton cloth wrapping for 24 hours), T5 (GA3 200 ppm), T6 (FYM treatment for 24 hours), T7 (FYM treatment for 48 hours), T8 (Hot water treatment) and T9 (Cow dung treatment for 24 hours). Experiment was laid out in CRD. The data recorded on the various parameters were analyzed at 5% level of significance by using ANOVA.Result: Results indicated highest germination percentage (51), lowest mortality percent (49), minimum days for initiation of germination (23), minimum days taken for germination completion (33), highest speed of germination (3.77), maximum vigour index (1441.44), highest root (11.27 cm) and highest shoot length (17 cm) in T5 (GA3 200 ppm) followed by T3 (water soaking for 48 hours). Indicating, T5 (GA3 200 ppm) to be best seed treatment for higher germination and subsequent seedling growth of Sarpagandha (Rauvolfia serpentine L.).

scholarly journals Studies on effects of seed size on germination and seedling growth of Elaeocarpus and Canarium tree species

2020 ◽  
Vol 16 (2) ◽  
pp. 270-276
Author(s):  
Basavaraj L. Tamadaddi ◽  
G. Prabhuling
Keyword(s):  
Seed Germination ◽  
Seed Size ◽  
Seedling Growth ◽  
Tree Species ◽  
Seed Treatment ◽  
Growth Parameters ◽  
Shoot Length ◽  
Medium Size ◽  
Dry Matter Accumulation ◽  
Collar Diameter

An investigation was undertaken on two medicinally important evergreen tree species of Western Ghats namely, Elaeocarpus munronii and Canarium striuctum to improve seed germination and enhance seedling growth in nursery. The seeds collected from two randomly chosen trees in each species were used for the study. In general seed germination was very poor in both the species. The maximum germination of 4.66 per cent was observed in Elaeocarpus munronii and it was 13.33 per cent in Canarium striuctum. Though big and medium size seeds recorded higher germination per cent than small size seeds in both the species studied, the seed size effect on germination was found to be non-significant. The results of the study revealed that seed size did influence seedling growth in both the species. Seeds of bigger size produced seedlings with higher values of shoot length, root length and collar diameter but their effect on dry matter accumulation was not significant. The effect of seed treatments on seedling growth was not noticed in Elaeocarpus munronii while seed treatment with sulphuric acid in Canarium striuctum showed slight but significant increase in shoot length, however, other growth parameters remained unaffected. The effects of seed size as well as of seed treatment on germination and seedling growth was found to be similar in both the trees species studied.

scholarly journals Effects of sodium hypochlorite on some physiological and cytogenetical parameters in Allium cepa L. exposed to salt stress

2019 ◽  
Vol 48 (2) ◽  
pp. 223-229 ◽  
Author(s):  
Kürşat Çavuşoğlu ◽  
Fadime Doğu ◽  
Dilek Çavuşoğlu
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Sodium Hypochlorite ◽  
Seedling Growth ◽  
Chromosomal Aberrations ◽  
Root Tip ◽  
Fresh Weight ◽  
Allium Cepa L

The effects of sodium hypochlorite (NaClO) on the seed germination, seedling growth (radicle length, radicle number and fresh weight), mitotic activity and chromosomal aberrations of Allium cepa L. germinated under salt stress were studied. Salt stress considerably inhibited the seed germination and seedling growth of A. cepa. Furthermore, it markedly reduced the mitotic index in root tip meristems of the seeds and increased the number of chromosomal aberrations. Whereas, the detrimental effects of salt on the seed germination, seedling growth, mitotic activitiy and chromosomal aberrations were dramatically alleviated in varying degrees by NaClO application.

scholarly journals Investigation of Seed Treatments for Management of Bacterial Leaf Spot of Lettuce

Plant Disease ◽  
2002 ◽  
Vol 86 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Ken Pernezny ◽  
Russell Nagata ◽  
Richard N. Raid ◽  
Janice Collins ◽  
Amanda Carroll
Keyword(s):  
Hydrogen Peroxide ◽  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Seed Treatment ◽  
Xanthomonas Campestris ◽  
Lettuce Seed ◽  
Copper Hydroxide ◽  
Seed Treatments ◽  
Seedborne Inoculum ◽  
Soil Mix

Chemical seed treatments were evaluated for efficacy of disinfestation of lettuce seed that had been inoculated with Xanthomonas campestris pv. vitians. Three concentrations of each chemical were evaluated by treating seed lots for 5 or 15 min. In addition, the effects of each seed treatment on seed germination and early plant growth were examined by observing seed germination rates. Bacteria were not detected when seed were treated with 3 or 5% hydrogen peroxide for 5 or 15 min. Treatment of seed with 0.52% sodium hypochlorite was relatively ineffective at 5 and 15 min. When sodium hypochlorite was used at a 1% concentration for 15 min, the level of bacterial infestation was reduced to 2%. Suspensions of copper hydroxide plus mancozeb also reduced seedborne inoculum to ≤2%. Treatment of seed with copper hydroxide alone, benzoyl peroxide, or calcium peroxide did not reduce seed infestation levels significantly. Seed germination rates were 90% or greater for the majority of seed treatments tested in laboratory assays. Hydrogen peroxide treatments at a concentration of 5% reduced seed germination up to 28% compared with controls. However, no significant differences in germination were observed among control treatments (noninoculated, nontreated seed and inoculated, nontreated seed) and any of the chemical seed treatments when seed were sown in a pasteurized soil mix in the greenhouse.

Effects of five different salts on seed germination and seedling growth of Haloxylon ammodendron (Chenopodiaceae)

2004 ◽  
Vol 14 (4) ◽  
pp. 345-353 ◽  
Author(s):  
Kazuo Tobe ◽  
Xiaoming Li ◽  
Kenji Omasa
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Seed Germination ◽  
Seedling Growth ◽  
Saline Soils ◽  
Initial Growth ◽  
Salt Solutions ◽  
Haloxylon Ammodendron ◽  
Low Concentration ◽  
Low Concentrations

Saline soils contain multiple types of salt, each of which may exert a different effect on seed germination and seedling growth of plants. The effects of five types of salt on the initial growth of Haloxylon ammodendron, a shrub found on both saline and non-saline areas in deserts of China, were investigated. Seeds were incubated at 20°;C in the dark in a solution (0 to –5.1 MPa) of a salt (NaCl, MgCl2, CaCl2, Na2SO4 or MgSO4) or polyethylene glycol (PEG)-6000, or in a salt (NaCl or MgCl2) or PEG solution containing a low concentration of CaCl2. Seed germination, seedling growth and cation (Na+, Mg2+, Ca2+ and K+) contents of seedlings were examined. Each salt had a different effect on seed germination, seedling growth and influx and outflux of cations in the seedlings. In both NaCl and MgCl2 treatments, the addition of low concentrations of CaCl2 favoured seed germination and seedling growth, and reduced K+ outflux from seedlings, but caused no appreciable decrease in the influx of Na+ or Mg2+ into seedlings. Marked abnormalities in seedlings were found only in treatments with Mg2+ salts, but these effects were completely alleviated by a low concentration of Ca2+ (Ca2+/Mg2+ = 0.012). The different responses of the initial growth in H. ammodendron to different isotonic salt solutions were attributed to differences among salt components in membrane permeability, toxicity and effects on functions of the plasma membrane and/or the cell wall.

scholarly journals Overcoming dormancy in Bauhinia scandens seeds

2020 ◽  
Vol 41 (4) ◽  
pp. 1189
Author(s):  
Tatiane Sanches Jeromini ◽  
Thais Soares Pereira ◽  
Cibele Chalita Martins ◽  
Givanildo Zildo da Silva
Keyword(s):  
Seed Germination ◽  
Water Content ◽  
Seedling Growth ◽  
Relative Frequency ◽  
Dry Matter ◽  
Hot Water ◽  
Initial Growth ◽  
Germination Process ◽  
Mean Germination Time ◽  
Medicinal Properties

Bauhinia scandens, an ornamental climbing vine, recently introduced in Brazil, in 2006 which has been gaining prominence due to its anti-tumor medicinal properties. A common feature amongst species of the Fabaceae family is dormancy due to the impermeability of the integument of the seed to water. The demand for seedlings of this species makes it necessary to elucidate the germination process and initial seedling growth. The objective of this study was to determine a method to overcome dormancy, thereby promoting seed germination and initial growth of B. scandens seedlings. The seeds were subjected to one of four treatments, besides the control: mechanical scarification, immersion in water (26 °C for 24 hours), immersion in hot water (86 °C for 24 hours, then 26 °C) and immersion in H2SO4 (95%) for 15, 30, 45, and 60 minutes. The seeds were evaluated for water content, germination (normal, abnormal seedlings, dormant and dead seeds), first count, mean germination time and relative frequency of germination. The seedlings were evaluated for emergence in sand, first count, emergence speed index, length and dry matter mass. B. scandens seeds should be immersed in H2SO4 for 45 and 60 minutes before sowing to overcome dormancy, in order to promote and standardize seed germination and enhance initial growth of the seedlings.

scholarly journals Production of desert rose seedlings in different potting media

2017 ◽  
Vol 23 (3) ◽  
pp. 250 ◽  
Author(s):  
Ronan Carlos Colombo ◽  
Vanessa Favetta ◽  
Deived Uilian de Carvalho ◽  
Maria Aparecida da Cruz ◽  
Sergio Ruffo Roberto ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Pine Bark ◽  
Initial Growth ◽  
Coconut Fiber ◽  
Potting Media ◽  
Emergence Percentage

Over the past decade the desert rose received fame in the flower market due to its striking and sculptural forms; however, the commercial production of these species is quite recent and little is known about its crop management, including substrates recommendation. The objectives of this study were to investigate the effect of different substrates on desert rose seed germination and production of its seedlings. Experiment I: freshly harvested seeds of desert rose were sown in different substrates e.g. sand, coconut fiber, semi-composted pine bark, sand + coconut fiber, semi-composted pine bark + sand and coconut fiber + semicomposted pine bark. These substrates were evaluated to study the emergence percentage of seeds, initial growth of seedlings and seedling emergence speed index (ESI). Experiment II: desert rose from the experiment I were transferred to plastic pots filled with the same substrates as in experiment I. The pH and electrical conductivity (EC) of the substrates were noted every 30 days while the growth parameters of seedlings were recorded after 240 days. Results from experiment I showed higher germination rate and seedling growth in substrates containing semi-composted pine bark. Similarly, in experiment II, better quality seedlings were observed in substrates containing semi-composted pine bark. Thus, for desert rose seed germination and seedling growth, it is recommended to use substrates containing semi-composted pine bark.

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