Effects of treatment with polysulfated glycosaminoglycan on serum cartilage oligomeric matrix protein and C-reactive protein concentrations, serum matrix metalloproteinase-2 and -9 activities, and lameness in dogs with osteoarthritis

Abstract The objective of this trial was to develop an index system to identify inflammation in Labrador Retrievers using a pressure walkway system. Gait analysis data can be difficult to interpret between treatment groups or for identifying low grade inflammation. To calculate the Total Inflammation Index™, the distance away from the ideal score was calculated for four parameters for each dog, including gait lameness score, total pressure index, step/stride ratio, and hind reach. These values were equally weighted and added together to produce the Total Inflammation Index™. For validation, the Total Inflammation Index™ values were compared to biomarker data for inflammation including cartilage oligomeric matrix protein, interleukin-6, creatine kinase, and c-reactive protein. Forty Labrador Retrievers (20 male/20 female) were used in this trial. All dogs were passed over the pressure walkway (Gait4Dogs; CIR Systems, Inc) to obtain gait analysis at baseline, 24h prior to the first 5km run, 24h after the first 5km run, 24h prior to the final 16km run, and 24h after the final 16km run. All biomarkers and the Total Inflammation Index™ were both significantly lower at the pre-exercise timepoints and elevated after post-exercise timepoints (P < 0.01). The Total Inflammation Index™ had significant correlation between timepoints and all biomarkers, including cartilage oligomeric matrix protein (P < 0.01), interleukin-6 (P < 0.05), creatine kinase (P < 0.01), and c-reactive protein (P < 0.05). The Total Inflammation Index™ appears to be a valid assay to evaluate generalized inflammation in Labrador Retrievers, and is in agreement with inflammatory biomarker values.

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Meta-analysis of serum C-reactive protein and cartilage oligomeric matrix protein levels as biomarkers for clinical knee osteoarthritis

BMC Musculoskeletal Disorders ◽

10.1186/s12891-018-1932-y ◽

2018 ◽

Vol 19 (1) ◽

Cited By ~ 5

Author(s):

Junfeng Zhang

Keyword(s):

Knee Osteoarthritis ◽

Cartilage Oligomeric Matrix Protein ◽

Matrix Protein ◽

Meta Analysis ◽

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein ◽

And Cartilage

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C-reactive protein increases matrix metalloproteinase-2 expression and activity in cultured human vascular smooth muscle cells

Journal of Laboratory and Clinical Medicine ◽

10.1016/j.lab.2005.07.010 ◽

2005 ◽

Vol 146 (5) ◽

pp. 287-298 ◽

Cited By ~ 28

Author(s):

Gabriella Doronzo ◽

Isabella Russo ◽

Luigi Mattiello ◽

Mariella Trovati ◽

Giovanni Anfossi

Keyword(s):

Smooth Muscle ◽

Matrix Metalloproteinase ◽

Vascular Smooth Muscle ◽

Smooth Muscle Cells ◽

Vascular Smooth Muscle Cells ◽

Muscle Cells ◽

Matrix Metalloproteinase 2 ◽

C Reactive Protein ◽

Reactive Protein ◽

Expression And Activity

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Relationship of osteopontin, matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-1, C-reactive protein and target organ damage in patients with essential hypertension

International Journal of Cardiology ◽

10.1016/j.ijcard.2009.09.112 ◽

2009 ◽

Vol 137 ◽

pp. S34

Author(s):

TINGYAN XU ◽

YANXIU LI ◽

SHENGNAN WU ◽

WENQUN NIU ◽

DINGLIANG ZHU ◽

...

Keyword(s):

Essential Hypertension ◽

Matrix Metalloproteinase ◽

Target Organ Damage ◽

Organ Damage ◽

Target Organ ◽

Tissue Inhibitor Of Metalloproteinase ◽

Matrix Metalloproteinase 2 ◽

C Reactive Protein ◽

Reactive Protein ◽

Relationship Of

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Association of Circulating COMP and YKL-40 as Markers of Metabolic Changes of Cartilage with Adipocytokines in Juvenile Idiopathic Arthritis

Metabolites ◽

10.3390/metabo10020061 ◽

2020 ◽

Vol 10 (2) ◽

pp. 61

Author(s):

Katarzyna Winsz-Szczotka ◽

Kornelia Kuźnik-Trocha ◽

Anna Gruenpeter ◽

Magdalena Wojdas ◽

Klaudia Dąbkowska ◽

...

Keyword(s):

Juvenile Idiopathic Arthritis ◽

Clinical Improvement ◽

Cartilage Oligomeric Matrix Protein ◽

Matrix Protein ◽

Metabolic Changes ◽

C Reactive Protein ◽

Human Cartilage ◽

Reactive Protein ◽

Erythrocyte Sedimentation ◽

Before And After

The aim of this study was to evaluate the association of circulating cartilage oligomeric matrix protein (COMP) and human cartilage glycoprotein-39 (YKL-40) as markers of metabolic changes of cartilage, with leptin, adiponectin, and resistin in juvenile idiopathic arthritis (JIA) patients before and after treatment. A significant decrease of COMP and an increase of YKL-4 were found in blood of untreated patients. JIA treatment leading to clinical improvement resulted in normalization of COMP levels only. Concentrations of both markers in treated patients, while showing no clinical improvement, differed from those in controls and patients with remission. The leptin level decreased (p < 0.05) in untreated patients; however, concentrations of adiponectin and resistin increased (p < 0.05) as compared to controls. JIA treatment resulted in normalization of adipocytokine levels in remissive patients but not those with active JIA. Untreated patients showed a correlation between COMP and leptin, adiponectin, and body mass index (BMI) and between YKL-40 and leptin, adiponectin, BMI, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR). In inactive JIA, a correlation between YKL-40 and leptin was shown. Treated patients with an active JIA demonstrated a correlation between COMP and adiponectin and between YKL-40 and leptin, adiponectin, BMI, CRP, and ESR. The results of this work indicate that leptin and adiponectin but not resistin may be involved in the development and progression of joint dysfunction in JIA. Additionally, we suggest that YKL-40 may be a useful biomarker of disease activity and may be used to assess treatment towards remission, as compared to COMP.

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High Serum Cartilage Oligomeric Matrix Protein Determines the Subset of Patients with Early-Stage Rheumatoid Arthritis with High Serum C-Reactive Protein, Matrix Metalloproteinase-3, and MRI-Proven Bone Erosion

The Journal of Rheumatology ◽

10.3899/jrheum.080926 ◽

2009 ◽

Vol 36 (6) ◽

pp. 1126-1129 ◽

Cited By ~ 20

Author(s):

KEITA FUJIKAWA ◽

ATSUSHI KAWAKAMI ◽

MAMI TAMAI ◽

MASATAKA UETANI ◽

SHOICHIRO TAKAO ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Cartilage Oligomeric Matrix Protein ◽

Matrix Protein ◽

Bone Erosion ◽

Early Stage ◽

High Serum ◽

C Reactive Protein ◽

Matrix Metalloproteinase 3 ◽

Reactive Protein ◽

Mri Features

Objective.To identify the significance of serum cartilage oligomeric matrix protein (COMP), a marker of cartilage turnover, in patients with early-stage rheumatoid arthritis (RA) in relation to other serologic variables and magnetic resonance imaging (MRI) features.Methods.Ninety-eight patients with early-stage RA, whose disease duration from onset was less than 2 years, were enrolled. The objective measures at baseline were Disease Activity Score (DAS28), serum C-reactive protein (CRP), serum matrix metalloproteinase-3 (MMP-3), serum antibodies against cyclic citrullinated peptide (anti-CCP), and MRI features of both wrist and finger joints. The MRI features included the number of sites scored positive for synovitis, bone edema, and bone erosion.Results.Serum COMP concentration was not different among groups identified with low, moderate, and high DAS28-CRP values. However, COMP values were statistically high in subjects positive for bone erosions on MRI compared with the subjects who were negative for bone erosions. A positive correlation of COMP with CRP and with MMP-3 values was also identified.Conclusion.Elevation of COMP may reflect joint damage that is dependent on the synovial inflammatory process in early-stage RA.

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