PSV-B-28 Late-Breaking: Development and validation of a Total Inflammation Index™ for identifying inflammation in Labrador Retrievers using a pressure walkway

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 383-384
Author(s):  
Jessica L Varney ◽  
Craig N Coon
Keyword(s):  
Creatine Kinase ◽  
Gait Analysis ◽  
Interleukin 6 ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Low Grade ◽  
C Reactive Protein ◽  
Inflammatory Biomarker ◽  
Reactive Protein ◽  
Labrador Retrievers

Abstract The objective of this trial was to develop an index system to identify inflammation in Labrador Retrievers using a pressure walkway system. Gait analysis data can be difficult to interpret between treatment groups or for identifying low grade inflammation. To calculate the Total Inflammation Index™, the distance away from the ideal score was calculated for four parameters for each dog, including gait lameness score, total pressure index, step/stride ratio, and hind reach. These values were equally weighted and added together to produce the Total Inflammation Index™. For validation, the Total Inflammation Index™ values were compared to biomarker data for inflammation including cartilage oligomeric matrix protein, interleukin-6, creatine kinase, and c-reactive protein. Forty Labrador Retrievers (20 male/20 female) were used in this trial. All dogs were passed over the pressure walkway (Gait4Dogs; CIR Systems, Inc) to obtain gait analysis at baseline, 24h prior to the first 5km run, 24h after the first 5km run, 24h prior to the final 16km run, and 24h after the final 16km run. All biomarkers and the Total Inflammation Index™ were both significantly lower at the pre-exercise timepoints and elevated after post-exercise timepoints (P < 0.01). The Total Inflammation Index™ had significant correlation between timepoints and all biomarkers, including cartilage oligomeric matrix protein (P < 0.01), interleukin-6 (P < 0.05), creatine kinase (P < 0.01), and c-reactive protein (P < 0.05). The Total Inflammation Index™ appears to be a valid assay to evaluate generalized inflammation in Labrador Retrievers, and is in agreement with inflammatory biomarker values.

Carbohydrate Influences Plasma Interleukin-6 but Not C-Reactive Protein or Creatine Kinase Following a 32-Km Mountain Trail Race

2006 ◽  
Vol 16 (1) ◽  
pp. 36-46 ◽  
Author(s):  
Mary P. Miles ◽  
Erin E. Walker ◽  
Stephen B. Conant ◽  
Shelly P. Hogan ◽  
Jessy R. Kidd
Keyword(s):  
Creatine Kinase ◽  
Interleukin 6 ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
C Reactive Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Ad Libitum ◽  
Voluntary Intake ◽  
Exercise Induced

Attenuation of exercise-induced interleukin-6 (IL-6) responses by carbohydrate (CHO) has been demonstrated in studies comparing controlled doses (≥ 0.9 g · kg−1 · h−1) to placebo, but not in studies of voluntary intake. This study sought to determine if attenuation of the IL-6 response during a 32.2-km mountain trail race occurs for high compared to low ad libitum CHO intakes. IL-6, C-reactive protein (CRP), and creatine kinase activity (CK) were analyzed from blood samples collected 12 h pre-, 0, 4, and 24 h post-race. Subjects were grouped into low (n = 14, 0.4 ± 0.1 g · kg−1· h−1) and high (n = 18, 0.8 ± 0.2 g · kg−1 · h−1) CHO intake groups. IL-6 0 h post-race (P < 0.05) was higher in the low (40.2 ± 22.7 pg · mL−1) compared to the high CHO group (32.7 ± 22.1 pg · mL−1). CRP and CK both increased post-race, but no differences were observed between groups. Attenuation of exercise-induced IL-6 is apparent across a range of CHO intakes.

Is there any role of interleukin-6 and high sensitive C-reactive protein in predicting IVF/ICSI success? A prospective cohort study

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
A. Seval Ozgu-Erdinc ◽  
Ilay Gozukara ◽  
Serkan Kahyaoglu ◽  
Saynur Yilmaz ◽  
Omer Hamit Yumusak ◽  
...  
Keyword(s):  
Cohort Study ◽  
Prospective Cohort Study ◽  
Interleukin 6 ◽  
Prospective Cohort ◽  
Clinical Pregnancy ◽  
Low Grade ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Icsi Cycle ◽  
Hs Crp

Abstract Objectives Studies have established a relationship between proinflammatory factors and implantation failure in IVF/ICSI cycles. Likewise, low-grade chronic inflammation is generally blamed for predisposing infertility. In the present study, we aimed to find a relationship between serum IL-6 and hs-CRP levels and IVF/ICSI cycle outcomes. Methods A total of 129 patients who consented to participate and attended the IVF unit of our department for the treatment of infertility have been enrolled in this prospective cohort study. Serum levels of high sensitive C-reactive protein and interleukin 6 have been detected at the beginning of the IVF/ICSI ovulation induction cycle. Cycle outcomes have been compared between patients with and without clinical pregnancy achievement following ART treatments. IVF/ICSI cycle outcomes of these two groups were also comparable except the number of >14 mm follicles, retrieved oocytes, metaphase II oocytes, and fertilized oocytes (2 pronuclei) which were in favor of the clinical pregnancy group. Results Mean serum hs-CRP levels were 3.08 mg/L (0.12–35.04) and 2.28 mg/L (0.09–22.52) patients with and without clinical pregnancy respectively. Mean serum IL-6 levels were 2 pg/mL (1–10.2) and 2 pg/mL (1–76.9) patients with and without clinical pregnancy respectively. Both tests were found to be statistically insignificant in predicting the success of the ART cycle in terms of implantation, clinical pregnancy, miscarriage, and live birth. Conclusions In the present study, we have not found any significant effect of hs-CRP and IL-6 levels in the IVF cycle. However, in the light of this and previous studies, large-scale research may prove the exact influence of these markers on IVF success.

scholarly journals The Use of Liraglutide in Obese Patients With COVID-19: A Case Report

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A38-A39
Author(s):  
Taliê Zanchetta Buani Hanada ◽  
Rafael Silvestre Knack ◽  
Renata Silvestre Knack
Keyword(s):  
Case Report ◽  
General Condition ◽  
Interleukin 6 ◽  
Laboratory Tests ◽  
Low Grade ◽  
Obese Patients ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Good General Condition ◽  
Anti Inflammatory

Abstract Introduction: In December 2019, a new type of coronavirus was discovered in Wuhan, China, characterized by a picture of atypical pneumonia composed of fever, dry cough and progressive dyspnea. Autopsy analyzes of patients with Covid-19 were performed, and hyperactivation of cytotoxic T cells was observed, suggesting an increase in humoral-type immunological signaling, where interleukin 6 (IL-6) is a mediator present that can fit as a potential critical agent for exacerbation of inflammatory conditions. In addition, not only interleukin-6, but also serum C-reactive protein (CRP) and ferritin have been recognized as strong predictors of COVID-19 severity. Recent studies have shown that the use of liraglutide has antioxidant and anti-inflammatory effects in vitro. Thus, the present case report discusses the possible anti-inflammatory properties of the antidiabetic drug liraglutide (Saxenda), in Covid-19. Clinical Case: JCMS, 45 years, male, married, obese grade 2 (BMI: 38.2), sought medical service on August 18, 2020, bringing a positive result of the RT-PCR test for Covid-19, performed in last day. The patient was in good general condition, reporting mild headache and adynamia. He was prescribed to start a treatment with Saxenda (6mg/ml - started 0.6mg/day at week 1, with a gradual increase up to 3mg/day at week 5); Jardiance (25mg / day); Fluimicil (600mg); Ivermectin (6mg) and Colchicine (0.5mg every 12 hours). In addition, collection of laboratory tests was requested. Examination results: IL6: &lt;1.5 pg/mL; Ferritin: 819 ng/ml; C-reactive protein: 5.1mg/L. On August 24, the patient was tachycardic (HR 120–140 bpm) associated with headache and fever (38 °C). Azithromycin (500mg), dexamethasone (4mg) and dipyrone (1g) were prescribed. Collection of laboratory tests was requested. Examination results: IL6: 9.3 pg/mL; Ferritin: 1085 ng/ml; C-reactive protein:23.9mg/L. On September 3, the patient was in good general condition, eupneic, afebrile, with no complaints to declare. On September 6, the collection of laboratory tests was requested. Examination results: IL6: &lt;1.5 pg/mL; Ferritin: 687 ng/ml; C-reactive protein: 1.7mg/L. Conclusion: Based on the described report, it is possible to observe a good clinical and laboratory evolution of the patient with Covid-19 who, among the drugs used, made use of liraglutida. Diabetes and obesity are considered significant risk factors for morbidity and mortality by COVID-19, since they are a condition of low-grade chronic inflammation and in these conditions, inflammatory markers such as CRP, IL-6 and ferritin have strong signs of alteration. Thus, the possible beneficial effect of the administration of liraglutide in obese patients is highlighted, as a potential anti-inflammatory effect, especially in the COVID-19 era.

scholarly journals Association of C-reactive protein and interleukin-6 with new-onset fatigue in the Whitehall II prospective cohort study

2012 ◽  
Vol 43 (8) ◽  
pp. 1773-1783 ◽  
Author(s):  
H. J. Cho ◽  
M. Kivimäki ◽  
J. E. Bower ◽  
M. R. Irwin
Keyword(s):  
Cohort Study ◽  
Interleukin 6 ◽  
Large Scale ◽  
Short Form ◽  
Low Grade ◽  
Continuous Variables ◽  
C Reactive Protein ◽  
Phase 3 ◽  
Reactive Protein ◽  
New Onset

BackgroundAlthough basic research on neuroimmune interactions suggests that inflammatory processes may play a role in the development of fatigue, population-based evidence on this association is limited. This study examined whether plasma C-reactive protein (CRP) and interleukin-6 (IL-6), biomarkers of systemic inflammation, predict fatigue onset.MethodThe Whitehall II study is a large-scale cohort study conducted in 20 civil service departments in London. Plasma CRP and IL-6 were measured in 4847 non-fatigued participants at phase 3 (1991–1993, aged 39–63 years). Fatigue was assessed using the Vitality subscale of the 36-item Short Form Health Survey (SF-36) at phase 3 and phase 4 (1995–1996).ResultsDuring a mean follow-up of 3.1 years, 957 new fatigue cases (19.7%) were identified using the pre-established cut-off score of ⩽50 on the Vitality subscale. CRP values were dichotomized as low (<1.0 mg/l ) or high (⩾1.0 mg/l) using the Centers for Disease Control/American Heart Association recommendations. Similarly, IL-6 values were also dichotomized as low (<1.5 pg/ml) or high (⩾1.5 pg/ml). After full adjustment for sociodemographic and biobehavioral covariates, the odds ratios for new-onset fatigue were 1.28 [95% confidence interval (CI) 1.09–1.49,p = 0.003] for high CRP and 1.24 (95% CI 1.06–1.45,p = 0.008) for high IL-6. Similar results were found when CRP and IL-6 were treated as continuous variables.ConclusionsPlasma CRP and IL-6 were prospectively associated with new-onset fatigue, supporting the hypothesis that low-grade inflammation has a role in the development of fatigue.

scholarly journals Interleukin-6 and highly sensitive C-reactive protein in obese adolescents

2012 ◽  
Vol 52 (4) ◽  
pp. 219
Author(s):  
Michael Kasenda ◽  
Suryadi Tantura ◽  
Sarah Warouw
Keyword(s):  
Interleukin 6 ◽  
Cross Sectional Study ◽  
Low Grade ◽  
C Reactive Protein ◽  
Tissue Mass ◽  
Cross Sectional ◽  
Obese Adolescents ◽  
Positive Correlation ◽  
Reactive Protein ◽  
Inflammatory State

Background Childhood obesity is a major health concern.Obesity is due to an expansion of adipose tissue mass. This tissueproduces pro􀁞inflarmnatory cytokines, such as interleukin􀁞6 (IL--6).IL􀁞6 is considered to be the chief stimulator of the production ofhighly sensitive C􀁞reactive protein (hsCRP) in the liver. Bothmolecules are responsible for the chronic low􀁞grade inflammatorystate in obese individuals.Objective To assess a correlation between IL􀁞6 and hsCRP inobese adolescents.Methods This cross􀁞sectional study was conducted from Marchto June 2011 in Manado. Subjects were obese and normal bodymass index (BMI) teens aged 13􀁞18 years. Serum glutamicoxaloacetic transaminase (SOOT) and serum glutamic pyruvictransaminase (SOPT) levels were measured to rule out liverimpairment. IL􀁞6 and hsCRP levels were also measured. Data wasanalyzed by Pearson's correlation and linear regression to test forcorrelation between IL--6 and hsCRP levels.Results There was a strongly positive correlation between IL􀁞6and hsCRP levels in obese adolescents (r=0.79 with P<O.OOl).IL--6 and hsCRP levels were not significantly associated in subjectswith normal BMI.Conclusions There was a strongly positive correlation betweenIL--6 and hsCRP levels in obese adolescents, suggestive of anongoing, chronic, low􀁞grade inflammatory state. [PaediatrIndanes.2012;52:219-22].

scholarly journals Association of Circulating COMP and YKL-40 as Markers of Metabolic Changes of Cartilage with Adipocytokines in Juvenile Idiopathic Arthritis

Metabolites ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 61
Author(s):  
Katarzyna Winsz-Szczotka ◽  
Kornelia Kuźnik-Trocha ◽  
Anna Gruenpeter ◽  
Magdalena Wojdas ◽  
Klaudia Dąbkowska ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Clinical Improvement ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Metabolic Changes ◽  
C Reactive Protein ◽  
Human Cartilage ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Before And After

The aim of this study was to evaluate the association of circulating cartilage oligomeric matrix protein (COMP) and human cartilage glycoprotein-39 (YKL-40) as markers of metabolic changes of cartilage, with leptin, adiponectin, and resistin in juvenile idiopathic arthritis (JIA) patients before and after treatment. A significant decrease of COMP and an increase of YKL-4 were found in blood of untreated patients. JIA treatment leading to clinical improvement resulted in normalization of COMP levels only. Concentrations of both markers in treated patients, while showing no clinical improvement, differed from those in controls and patients with remission. The leptin level decreased (p < 0.05) in untreated patients; however, concentrations of adiponectin and resistin increased (p < 0.05) as compared to controls. JIA treatment resulted in normalization of adipocytokine levels in remissive patients but not those with active JIA. Untreated patients showed a correlation between COMP and leptin, adiponectin, and body mass index (BMI) and between YKL-40 and leptin, adiponectin, BMI, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR). In inactive JIA, a correlation between YKL-40 and leptin was shown. Treated patients with an active JIA demonstrated a correlation between COMP and adiponectin and between YKL-40 and leptin, adiponectin, BMI, CRP, and ESR. The results of this work indicate that leptin and adiponectin but not resistin may be involved in the development and progression of joint dysfunction in JIA. Additionally, we suggest that YKL-40 may be a useful biomarker of disease activity and may be used to assess treatment towards remission, as compared to COMP.

High Serum Cartilage Oligomeric Matrix Protein Determines the Subset of Patients with Early-Stage Rheumatoid Arthritis with High Serum C-Reactive Protein, Matrix Metalloproteinase-3, and MRI-Proven Bone Erosion

2009 ◽  
Vol 36 (6) ◽  
pp. 1126-1129 ◽  
Author(s):  
KEITA FUJIKAWA ◽  
ATSUSHI KAWAKAMI ◽  
MAMI TAMAI ◽  
MASATAKA UETANI ◽  
SHOICHIRO TAKAO ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Bone Erosion ◽  
Early Stage ◽  
High Serum ◽  
C Reactive Protein ◽  
Matrix Metalloproteinase 3 ◽  
Reactive Protein ◽  
Mri Features

Objective.To identify the significance of serum cartilage oligomeric matrix protein (COMP), a marker of cartilage turnover, in patients with early-stage rheumatoid arthritis (RA) in relation to other serologic variables and magnetic resonance imaging (MRI) features.Methods.Ninety-eight patients with early-stage RA, whose disease duration from onset was less than 2 years, were enrolled. The objective measures at baseline were Disease Activity Score (DAS28), serum C-reactive protein (CRP), serum matrix metalloproteinase-3 (MMP-3), serum antibodies against cyclic citrullinated peptide (anti-CCP), and MRI features of both wrist and finger joints. The MRI features included the number of sites scored positive for synovitis, bone edema, and bone erosion.Results.Serum COMP concentration was not different among groups identified with low, moderate, and high DAS28-CRP values. However, COMP values were statistically high in subjects positive for bone erosions on MRI compared with the subjects who were negative for bone erosions. A positive correlation of COMP with CRP and with MMP-3 values was also identified.Conclusion.Elevation of COMP may reflect joint damage that is dependent on the synovial inflammatory process in early-stage RA.

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