scholarly journals Rhizosphere Bacterial Communities of Arundo Donax Grown in Soil Fertilised with Sewage Sludge and Agricultural by-Products

2019 ◽  
Vol 65 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Katarína Ondreičková ◽  
Marcela Gubišová ◽  
Jozef Gubiš ◽  
Lenka Klčová ◽  
Miroslav Horník
Keyword(s):  
Sewage Sludge ◽  
Bacterial Communities ◽  
Statistical Tests ◽  
Treatment Plant ◽  
Intergenic Spacer ◽  
Principal Component ◽  
Arundo Donax ◽  
Toxic Substances ◽  
Molecular Fingerprinting ◽  
By Products

Abstract Application of sewage sludge to soil is a potentially inexpensive source of nutrition for plants, but may contain undesirable and toxic substances, e.g. heavy metals. Alterations in microbial communities can serve as an environmental indicator of possible soil contamination. We used two molecular fingerprinting methods (Automated Ribosomal Intergenic Spacer Analysis, ARISA and Terminal Restriction Fragment Length Polymorphism, T-RFLP) to study changes in the genetic diversity of bacterial communities in the rhizosphere of Arundo donax L. cultivated in the soil fertilised with additive based on sewage sludge from wastewater treatment plant and agricultural by-products represented by crushed corn hobs and wastes from grain mill industry. The metagenomic DNA extracted from rhizosphere samples were collected in August and November 2014. The amount of mgDNA was statistically higher in samples with additive than in control samples without it in both dates. The Venn diagrams showed that operational taxonomic units which were common to all samples were represented in 32.8% in ARISA and 43.4% in T-RFLP. However, based on Principal component analysis and subsequent PERMANOVA statistical tests did not confirm significant differences in the rhizosphere of control plants and plants grown in the soil supplemented with sewage sludge in dose 5 and 15 t/ha present in the additive.

scholarly journals Monitoring of Rhizosphere Bacterial Communities in Soil with Sewage Sludge Addition Using Two Molecular Fingerprinting Methods: Do These Methods Give Similar Results?

2016 ◽  
Vol 62 (2) ◽  
pp. 52-61 ◽  
Author(s):  
Katarína Ondreičková ◽  
Alžbeta Žofajová ◽  
Michaela Piliarová ◽  
Jozef Gubiš ◽  
Martina Hudcovicová
Keyword(s):  
Sewage Sludge ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Intergenic Spacer ◽  
Diversity Indices ◽  
Maturity Stage ◽  
Molecular Fingerprinting ◽  
Rhizosphere Bacterial Communities ◽  
Fingerprinting Methods ◽  
Soil Additive

Abstract In this study, bacterial genetic diversity from the rhizosphere of barley and wheat were studied. The plants were sown in pots with aliquot amount of 15 t/ha concentration of soil additive derived from sewage sludge and agricultural by-products represented by wastes from grain mill industry and crushed corn cobs. The plants sown in pots without the addition of soil additive represented control samples. The rhizosphere samples were collected on two dates (plant flowering and maturity) and the composition of bacterial communities were detected using two molecular fingerprinting methods – automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (T-RFLP). Microbial biomass expressed as the amount of metagenomics DNA was higher in soils with addition of soil additive, except during maturity stage in barley rhizosphere. Nevertheless, statistically significant differences between control and sludge samples were not detected in any case. Similarly, no changes were detected in the composition of bacterial community between control and sludge samples in barley and wheat rhizosphere by using cluster analysis. Only minor temporal changes in the composition of bacterial community between flowering and maturity periods were observed. These changes were related to the samples collected in the plant maturity stage. In this stage, plants were completely mature and their impact on the rhizosphere bacterial communities in the form of root exudates was limited. Statistically significant differences between ARISA and T-RFLP methods were detected in all measured values of diversity indices. Despite these differences, both methods gave results leading to similar conclusions.

Effects of high temperature melting on the porosity and microstructure of slags from domestic sewage sludge

2000 ◽  
Vol 41 (8) ◽  
pp. 99-105 ◽  
Author(s):  
Z. Abu-Kaddourah ◽  
A. Idris ◽  
M. J. Noor ◽  
F. R. Ahmadun
Keyword(s):  
High Temperature ◽  
Sewage Sludge ◽  
Thermal Treatment ◽  
Cooling Rate ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Domestic Sewage ◽  
Holding Time ◽  
By Products ◽  
Temperature Melting

Sewage treatment plant sludges continue to pose great problems in terms of volume, odour and method of disposal. Thermal treatment of sewage sludge is considered as an attractive method in reducing sludge volume, which at the same time produces reusable by-products. Studies on high temperature melting of sewage sludge, above 1200°C, show promising results where by stable and inert by-products are produced. This paper presents a part of a big project on thermal treatment of sewage sludge, carried out in University of Putra Malaysia, this part highlights the changes in the microstructure of domestic sewage sludge when subjected to different meltingprocedures; heating temperature, cooling rate, and holding time. The heating temperatures ranged between 1250°C–550°C, and the rate of cooling was between 2.5°C–10°C per minute. Using XRD analyses, itwas found that the melted slags were amorphous despite the different melting procedures applied. The SEM microphotographs indicated that some crystallization had occurred under the melting procedures; 1350°C with a cooling rate of 5°C per minute and a holding time of 45 minutes, 1400°C with a cooling rate of 2.5°C per minute and holding time of 45 minutes, and 1400°C with a cooling rate of 5°C per minute and without holding time.

scholarly journals Determination of the Bacterial Compositions in the Bio-Drying Process of Sewage Sludge from Wastewater

2021 ◽  
Author(s):  
Joseph Ikenna Ubah ◽  
Ogwueleka Tochukwu Chibueze ◽  
C. I Ofeoshi
Keyword(s):  
Sewage Sludge ◽  
Bacterial Communities ◽  
Treatment Plant ◽  
Free Water ◽  
Staining Technique ◽  
Organic Content ◽  
Wastewater Sludge ◽  
Water Evaporation ◽  
Drying Process ◽  
Plate Technique

Abstract The aim of this study is to determine the bacterial compositions during the bio-drying process of sewage sludge. Bio-dried products and sewage sludge were randomly collected from the Wupa wastewater/sludge treatment plant in Idu Industrial area Abuja, Federal Capital Territory, Nigeria. A mixture of sewage sludge and sawdust that were bio–dried and cured before this experiment were composition of the bio–dried product. The bacterial communities were analyzed in the laboratory using Pour Plate Technique to examine the total bacterial present in the sewage sludge bio-drying material (SSBM) and the bacterial were later isolated using other techniques like Oxidase test, Catalase test, Grams Staining technique and Spread Plate Technique. Evaporation of free water and water formation was determined by humidity gauge and moisture-oven drying method. From the results obtained, Acinetobacter was the most abundant bacterial during the initial and final thermophilic phases (43% and 37%) respectively. Bacillus was the most abundant amid the cooling stage (53%). The water evaporation and water generation rate were credited to the degradation of organic content of the SSBM and heat produced by bacterial activities. From this study, there is a clear indication that the bacterial density grades that increase the temperature of the SSBM during the bio–drying process reshaped the bacterial communities.

scholarly journals Within- and between-Lake Variability in the Composition of Bacterioplankton Communities: Investigations Using Multiple Spatial Scales

2004 ◽  
Vol 70 (1) ◽  
pp. 214-223 ◽  
Author(s):  
Anthony C. Yannarell ◽  
Eric W. Triplett
Keyword(s):  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Bacterial Communities ◽  
Spatial Scales ◽  
Bacterial Community Composition ◽  
Intergenic Spacer ◽  
Molecular Fingerprinting ◽  
Concentration Gradients ◽  
Lake Productivity ◽  
Organic Carbon Concentration

ABSTRACT This study examined the similarity of epilimnetic bacterial community composition (BCC) across several within- and among-lake spatial scales, and the environmental factors giving rise to similar bacterial communities in different lakes were also explored. Samples were collected from 13 northern and southern Wisconsin lakes representing gradients in lake size, productivity, dissolved organic carbon and humic acid contents, and pH. Hypotheses regarding patchy distribution of bacterial communities in lakes were tested by comparing samples collected from nearby (tens of meters) and distant (hundreds of meters) sampling sites in the same lake. BCC was characterized by using a molecular fingerprinting technique, automated ribosomal intergenic spacer analysis (ARISA). Overall, samples collected at the 10-m, 100-m, and between-lake scales differed by 13, 17, and 75%, respectively. Variation at these last two scales was significant. The development of within-lake variation in BCC appeared to depend on the isolation of water by lake shoreline features such as bays or narrow constrictions. ARISA profiles from northern lakes had fewer peaks and were less similar to each other than were those of the southern lakes, suggesting that regional features do not necessarily lead to the development of similar bacterial communities. Lakes at similar positions on productivity and dissolved organic carbon concentration gradients had similar bacterial communities, and bacterial diversity was positively correlated with lake productivity and water temperature. Factorial studies taking into account these gradients, as well as regional spatial scales, should provide much insight into the nature of aquatic bacterial biogeography.

EVALUATION OF BIOMETHANE YIELDS FROM HIGH-ENERGY ORGANIC WASTE AND SEWAGE SLUDGE: A PILOT STUDY FOR A WASTEWATER TREATMENT PLANT

2019 ◽  
Vol 18 (9) ◽  
pp. 2023-2034 ◽  
Author(s):  
Agnieszka A. Pilarska ◽  
Krzysztof Pilarski ◽  
Boguslawa Waliszewska ◽  
Magdalena Zborowska ◽  
Kamil Witaszek ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Pilot Study ◽  
Sewage Sludge ◽  
Wastewater Treatment Plant ◽  
Organic Waste ◽  
Treatment Plant ◽  
High Energy

Production of fuels on the basis of sewage sludge through conditioning using high calorific value fractions

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
Karl-Georg Schmelz ◽  
Anja Reipa ◽  
Hartmut Meyer
Keyword(s):  
Sewage Sludge ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Calorific Value ◽  
Fluidised Bed ◽  
Sludge Treatment ◽  
Heating Value ◽  
Fine Coal ◽  
Used Cars ◽  
Plastic Components

Emschergenossenschaft and Lippeverband operate 59 wastewater treatment plants which produce approx. 100,000 Mg TS of sewage sludge each year. Using sludge pressure pipelines, about 60 % of this sludge are transported to the central sludge treatment plant in Bottrop. The digested sludges are conditioned using fine coal and polymers and are dewatered using membrane filters. By adding coal, the heating value of the sludge is raised which enables autothermal combustion of the dewatered sludges in fluidised bed furnaces at the central sludge treatment plant. In order to replace coal, a fossil fuel, as conditioning agent, experiments were conducted using alternative materials with high heating values. The addition of shredder fluff agglomerates proved to be particularly successful. Shredder fluff agglomerates are a residue from the recycling of used cars and are generated in a multistage process (e.g. Volkswagen-SiCon Process) by separating the light shredder fraction (plastic components etc.) from the total shredder fluff. The fibrous material is outstandingly suitable for improving the dewaterability and for sufficiently raising the heating value of the dewatered sludge in order to enable autothermal combustion. Since first experiments showed very positive results, a full-scale long-term test-run will take place in 2007.

Sludge minimization by disintegration at different wastewater treatment plants

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
Luchien Luning ◽  
Paul Roeleveld ◽  
Victor W.M. Claessen
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
New Technologies ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Biological Degradation ◽  
Ongoing Research ◽  
Sludge Minimization

In recent years new technologies have been developed to improve the biological degradation of sewage sludge by anaerobic digestion. The paper describes the results of a demonstration of ultrasonic disintegration on the Dutch Wastewater Treatment Plant (WWTP) Land van Cuijk. The effect on the degradation of organic matter is presented, together with the effect on the dewatering characteristics. Recommendations are presented for establishing research conditions in which the effect of sludge disintegration can be determined in a more direct way that is less sensitive to changing conditions in the operation of the WWTP. These recommendations have been implemented in the ongoing research in the Netherlands supported by the National Institute for wastewater research (STOWA).

Development of a bio-monitoring system for toxicants in water with fish

2001 ◽  
Vol 1 (2) ◽  
pp. 9-17
Author(s):  
Y.-H. Lee ◽  
H.-K. Lee ◽  
C.-H. Chang ◽  
W.-H. Kim
Keyword(s):  
Monitoring System ◽  
Treatment Plant ◽  
Ccd Camera ◽  
Water Treatment Plant ◽  
Abnormal Behavior ◽  
Time Interval ◽  
Toxic Substances ◽  
Bio Monitoring ◽  
Index Value ◽  
A Charge

A bio-monitoring system for toxicants in water has been developed and verified for actual applications. This system is based on the motionality of five Acheilognathus lanceolata, a fish known to be very sensitive to toxic substances, moving around in an aquarium. Their movements are continuously monitored with a charge coupled device (CCD) camera and analyzed to find and quantify any abnormal behavior in their motional characteristics in comparison with the pre-acquired data. That is, the images of fish captured by a CCD camera are digitalized to identify the location of fish in a constant time interval and the locations of each fish were then analyzed mathematically with a personal computer using the equations proposed to determine the motional characteristics such as floatness, fledness and mobility(agility). These data are then converted by means of fuzzy estimation to an index value, defined as the contamination index (CI), by which the system provides the information about the overall toxic strength of the toxicant in the water flowing into the aquarium. If the fish are exposed to toxicant(s), the CI value will be proportional to the strength of its toxicity. The pilot test was performed in a water treatment plant for six months in order to verify the reproducibility of the system over the unstable conditions such as highly turbid water after rainfall as well as in normal conditions. The test results revealed that this monitoring system has good reproducibility and sensitivity, proving our approach, described in this paper, is reliable. As a result, this approach seems to enable us to make a quick and easy detection of toxic substances contained in water, therefore, this system can be applied to a source of water supply as a toxicant watching system.

Generic monitoring tools for water characteristics assessment

2003 ◽  
Vol 3 (1-2) ◽  
pp. 351-357
Author(s):  
S. Le Bonté ◽  
M.-N. Pons ◽  
O. Potier ◽  
S. Chanel ◽  
M. Baklouti
Keyword(s):  
Municipal Wastewater ◽  
Principal Component ◽  
Toxic Substances ◽  
Capacity Curves ◽  
Water Characteristics ◽  
Monitoring Tools ◽  
Visible Spectra ◽  
On Line ◽  
Mining Procedure

An adaptive principal component analysis applied to sets of data provided by global analytical methods (UV-visible spectra, buffer capacity curves, respirometric tests) is proposed as a generic procedure for on-line and fast characterization of wastewater. The data-mining procedure is able to deal with a large amount of information, takes into account the normal variations of wastewater composition related to human activity, and enables a rapid detection of abnormal situations such as the presence of toxic substances by comparison of the actual wastewater state with a continuously updated reference. The procedure has been validated on municipal wastewater.

Some Recent Developments in Sewage Sludge Pumping

1988 ◽  
Vol 20 (4-5) ◽  
pp. 227-236
Author(s):  
J. H. Lohmann ◽  
W. F. Garber
Keyword(s):  
Sewage Sludge ◽  
Treatment Plant ◽  
Rheological Characteristics ◽  
Volatile Content ◽  
Collection System ◽  
Positive Displacement ◽  
Low Viscosity ◽  
Recent Developments ◽  
Cylinder Piston ◽  
Pumping Equipment

The pumping of sewage sludge solids is reviewed in terms of handling high and low viscosity materials with solids concentrations varying from about 4 % to perhaps 50 %. Knowledge of rheological characteristics including thixotropy is necessary in designing pumping systems with sludge solids percentages, volatile content, particle size, abrasives content and temperatures being variables which could change pump types found suitable from facility to facility. The characteristics of a sewage collection system is also important in determining pumping parameters needed in a treatment plant. Solids handling equipment from other industries have made significant contributions to sewage solids pumping in recent years. Included are moyno-type progressing cavity screw-centrifugal, recessed impeller rotation piston positive displacement, and two cylinder piston S-transfer positive displacement pumps. Pumping equipment used in the F.R.G. and in Southern California in the U.S.A. is remarkably similar. These types of pumps offer the possibility of designing optimum solids dewatering and transfer systems.

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