scholarly journals Germination and Growth in Control and Primed Seeds of Pepper as Affected by Salt Stress

2014 ◽  
Vol 47 (3) ◽  
pp. 83-95 ◽  
Author(s):  
H. Aloui ◽  
M. Souguir ◽  
S. Latique ◽  
C. Hannachi

Abstract Salinity is an important abiotic stress which can affect crop production in the world. One of the simplest methods for improving salinity tolerance of plants is seeds priming. This experiment was conducted to evaluate the effects of seeds priming with three solutions (KCl , NaCl and CaCl2) in germination and later growth of three pepper (Capsicum annuum L.) cultivars: Beldi, Baklouti and Anaheim Chili. Seeds germination was conducted in a completely randomized design under seven salinity levels (0, 2, 4, 6, 8, 10 and 12 g L-1) at room temperature for primed and control seeds. Plants derived from these germinated seeds (control and primed) were transplanted and cultivated in a greenhouse for 4 months and were irrigated permanently with seven salinity levels (0, 2, 4, 6, 8, 10 and 12 g L-1). The results showed that salinity affected all parameters under study like total germination percentage and chlorophyll level (a and b). As well, proline content increased as response to increasing salinity. The plants derived and grown from primed seeds showed a considerable tolerance to salt stress and gave better results. In fact, priming improved the salt resistance of pepper owing to more chlorophyll and proline accumulation. These results suggest that seed priming induced possible physiological adjustments in pepper seeds, especially in the early stages of development, and could be used as a suitable tool for improving germination and growth characteristics under salt stress conditions.

2021 ◽  
pp. 76-82
Author(s):  
Getachew Shumye Adilu ◽  
Yohannes Gedamu Gebre

Salinity adversely affects 20-30% of the irrigated area in the world. Tomato is sensitive to salinity. It is one of the most severe abiotic factors of many agricultural crops and it becoming the main problem in Ethiopia. This study was conducted to evaluate the effects of different salinity levels on the seed germination parameters of tomato varieties. It was laid out in a completely randomized design with three replicates. The treatment included four tomato varieties (Sirinka, Weyno, ARP D2, and Roma VF) and five salinity levels (1 dS m-1, 2 dS m-1, 3 dS m-1, 4 dS m-1, and control). Fifty seeds were placed in a Petri dish over a moistened germination paper for germination and seedlings and allowed to grow for 14 days. The germination rate, speed and energy of tomato seeds were significantly (p < 0.001) affected by the combined effect of variety and salinity. The shortest mean germination time, the highest mean germination rate, and the highest speed of germination were recorded in the ARP D2 variety in the control treatment. The lowest first and last days of germination, and the uncertainty of germination were recorded from ARP D2. However, an increase in the days of germination and in the uncertainty of germination, and a decrease in the germination index and total germination percentage trends were observed with increasing salinity levels. The highest level of salinity (4 dS m-1) affected the germination of tomato varieties. Among the four tested tomato varieties, ARP D2 and Roma VF were tolerant to salinity.


2015 ◽  
Vol 7 (1) ◽  
pp. 96-101 ◽  
Author(s):  
Ali SEPEHRI ◽  
Saeed NAJARI ◽  
Hossein Reza ROUHI

In order to investigate the effect of hydro-priming on seed germination with distilled water on germination of five Alfalfa cultivars under salinity stress, an experiment was conducted as a factorial experiment based on a completely randomized design with three replications. Seven levels of hydro-priming and salinity of NaCl including prime and non-salinity, prime and 50 mM salinity, prime and 100 mM salinity, prime and 150 mM salinity, prime and 200 mM salinity, prime and 250 mM salinity and without prime and salinity and five alfalfa varieties, including ‘Hamedani’, ‘Isfahani’, ‘Bami’, ‘Yazdi’ and ‘Ghareh Yonjeh’ were used. The results showed that the main effect of prime, salinity and cultivars and their interaction in all studied traits were significantly affected at the 5% probability level. Priming treatments in non-salinity of all cultivars were the highest. In all cultivars, final germination percentage, length and weight of radicle, plumule and seedling, germination rate and time, relative radicle elongation, vigor index and stress index, were significantly improved in response to priming in salinity levels of 50-200 mM, compared to control. Radicle produced higher length and weight than the plumule in hydro- priming and salinity treatments. ‘Hamedani’ cultivar in most of studied characteristics had a better response than others. The lowest response to salinity stress and priming was observed in ‘Yazdi’ cultivar.


2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Muna A. Yousif ◽  
Nasseam .AH. Hassan ◽  
Thaer Turky Abdul kareim

"A factorial experiment with completely randomized design (CRD)with three replicates was carried out at agronomy laboratory of the Directorate of seed testing and certification / Branch of Salahaldeen to study the effect of salinity levels of irrigation water )0 ،1.75 and 2 Mm ) and grains affected by the use of mechanical seeds in seed germination and seedling growth for two varieties of wheat bread(IPA99 and Sham 6). The characteristics of germination percentage ،speed germination ،length of plumule and radical ،pure live seed percentage and seed vigor studied. The observed results are as follows: Significant differences are found between cultivars. (IPA99) cultivar gave the highest tolerance to water salinity and seed fracture. However ،salinity levels and seed fracture caused a significant decrease in all features. As for interactions ،cultivars and seed mechanical damage interactions were significant in all characteristics. While the interactions of varieties and levels of salinity were significant in the characteristics of the speed of germination and length of plumule . Interactions of salinity levels and seed mechanical damage did not reach the significant limit except for the characteristic of the plumule’s length, which reached the highest value at 7.697 cm . At the same time, the triple interactions (cultivars ،seed mechanical damage and levels of salinity) were significant except for both germination percentage and pure live seed percentage. The best triple interaction was (IPA99) with 1.75 Mm and undamaged seed, which gave the best values in: germination percentage ،length of plumule and radical ،pure live seed percentage and seed vigor. The values of the above were as follows: 98.67% ،8.707 cm ،10.01 cm ،94.72 ،18.45 respectively."


2018 ◽  
Vol 7 (4) ◽  
pp. 2139
Author(s):  
Savita J. ◽  
Somveer Jakhar

Salinity is one of the limiting environmental factors for crop production. Chickpea has special importance among the legumes especially in arid and semi-arid regions and is sensitive to salinity. Therefore, it becomes necessary to make a plan to mitigate the salinity effect on this plant. For this purpose, an experiment was conducted in the net house of Department of Botany, Kurukshetra University, Kurukshetra to investigate the role of sulfosalicylic acid (SSA) at different concentrations (10-4, 10-5 and 10-6 M) in overcoming salinity stress imposed on chickpea plants in natural conditions. Different salinity levels (0, 50 mM, 100 mM and 150 mM) were applied and caused a significant reduction in morphological and yield parameters. Our main findings are as follows: (1) Salt stress has detrimental effects on growth and physiology of plants. (2) Application of SSA at 10-5 M was the most significant concentration in modulating the inhibitory effects of salt stress.


2015 ◽  
Vol 67 (3) ◽  
pp. 993-1000 ◽  
Author(s):  
Lydia Shtereva ◽  
Roumiana Vassilevska-Ivanova ◽  
Tanya Karceva

An experiment was carried out hydroponically under laboratory conditions to investigate the effect of salt stress on several physiological and biochemical parameters of three sweet corn (Zea mays L. var. saccharata) genotypes: lines 6-13, C-6 (pollen source) and their heterotic F1 hybrid ?Zaharina?. The degree of salinity tolerance among these genotypes was evaluated at three different sodium chloride (NaCl) concentrations: 0 mM, 100 mM, 125 mM and 150 mM. Seed germination, plant growth and biochemical stress determining parameters such as malondialdehyde (MDA), proline content and hydrogen peroxide (H2O2) levels were compared between seedlings of lines and hybrid. The obtained results indicated that both lines and hybrid have similar responses at different salinity levels for all examined traits. All the seedlings? growth parameters, such as germination percentage, root length, shoot length, root and shoot fresh and dry weight, decreased with increasing salinity level. MDA, proline and H2O2 increased at different saline conditions in comparison to the control. Based on the results, of the three genotypes examined, the hybrid Zaharina, followed by line C-6, was more salt-sensitive than line 6-13 in salt stress condition.


Author(s):  
Panayiota PAPASTYLIANOU ◽  
Ilias TRAVLOS ◽  
Ioannis ROUSSIS ◽  
Dimitrios BILALIS

Teff is an annual grass originated in Ethiopia, grown for both human food and animal feed. Salinity and moisture stress are the main limiting factors of agricultural development on arid and semi-arid regions. The aim of this study was to investigate the effect of salinity due to NaCl on germination of teff. A set of experiments was conducted under a completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 25 °C) in the dark for 10 days. In order to create salt stress conditions during the germination process, seven solutions were used (0 as control, 80, 160, 240 and 320, 400 and 480 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The presence of NaCl reduced germination, especially above 240 mM for 15 °C and 400 mM for 25 °C. The mean germination time increased with higher NaCl levels.


2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.


2017 ◽  
Vol 15 (2) ◽  
pp. 182-187
Author(s):  
D Das ◽  
MA Ali ◽  
TA Sarkar ◽  
MY Ali

The experiment was conducted at the Agronomy Laboratory of Agrotechnology Discipline, KhulnaUniversity, Khulnato determine the effect of salt stress on germination and seedling growth of indigenous Aman rice varieties during June, 2015 to December, 2015.  The salt tolerant landraces will be used as genetic resource for variety development program in future. The factorial experiment consists of two factors such as ten indigenous Aman rice varieties and four levels of salt solutions viz EC (dS m−1) of 5, 10 and 15 with control (distilled water). The experiment was laid out in Completely Randomized Design (CRD) with three replications. The interaction effects between variety and salinity on germination and seedling growth parameters of indigenous Aman rice varieties were varied significantly. It was found that germination capacity, energy, speed, seedling vigor index, root length and shoot length of rice were decreased gradually with increasing salinity. The germination energy and germination speed of most varieties were drastically reduced at 10 dS m−1 or higher salinity levels. The germination capacity of Hatibazor, Boushohagi, Shadagotal and Moinamoti were recorded 97.66 %, 99.33%, 98.0 % and 97.33%, respectively at 15 dS m−1 water salinity in laboratory condition. The results revealed that the indigenous Aman rice varieties named Boushohagi, Shadagotal, Hatibazor, Moinamoti, Motha were performed better over other varieties considering of germination and seedling growth parameters.J. Bangladesh Agril. Univ. 15(2): 182-187, December 2017


2020 ◽  
Vol 33 (3) ◽  
pp. 853-859
Author(s):  
CARLA DE SOUZA ALMEIDA ◽  
HUGO ROLDI GUARIZ ◽  
MARÍLIA ALVES BRITO PINTO ◽  
MARINEIDE FERREIRA DE ALMEIDA

ABSTRACT Salt stress negatively affects plant development, mainly in arid and semiarid regions, promoting changes in their physiology and productivity. The objective of this study was to evaluate the germinative potential of creole maize (Zea mays L.) and fava bean (Phaseolus lunatus L.) seeds under different salt stress conditions. The seeds were collected in rural areas of the municipalities of Guanambi, Candiba, and Brumado, which are within the Serra Geral region in the state of Bahia, Brazil. A completely randomized experimental design was used; the treatments consisted of three salts (CaCl2, MgCl2, and NaCl) and four salinity levels (osmotic potentials of -0.3, -0.9, and -1.2 MPa). Seed physical analyses included moisture and purity, and physiological analyses included germination percentage, germination speed index, average germination time, and relative germination frequency. The storage of creole maize and fava bean seeds in plastic bottles and under adequate humidity ensures that seeds will have moisture content and physical purity within ideal values. Salt stresses induced by NaCl and CaCl2 salts at osmotic potentials of -0.3 to -1.2 MPa hinder the creole maize and fava bean seed germination, and their germination are null for both salts at osmotic potentials lower than -0.3 MPa. The creole maize and fava bean seeds show germination of 70% under salt stress induced by MgCl2 at osmotic potential of -0.3 MPa, making the germination of both species viable.


2014 ◽  
Vol 2 (2) ◽  
pp. 88-92 ◽  
Author(s):  
Jafar Abili ◽  
Sajjad Zare

Salinity is one of the major stresses in arid and semi-arid regions causing adverse effects at physiological, biochemical, and molecular levels, limiting crop productivity. In this research, three canola cultivars (Licord, Talayeh, Zarfam) were compared at 5 salinity levels (control, 50, 100, 150 and 200 mM) for their catalase, guaiacol peroxidase, superoxide dismutase activity,proline and yield in a completely randomized design with 3 replications. In our study, we found that NaCl concentrations greater than 150 and 200 mM caused the irreversible disorders. Increased salt concentrations led to significant changes in the levels of antioxidative enzymes and proline in three canola cultivars. Also, yield rates in three varieties decreased in the presence of NaCl concentrations.


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