Effect of Storage Containers and Lengths of Storage on the Germination, Moisture Content and Pest Infestation of Wheat Seed

2018 ◽  
Vol 51 (3) ◽  
pp. 13-23
Author(s):  
M. Golam Azam ◽  
M. Sohidul Islam ◽  
K. Hasan ◽  
M. Kaum Choudhury ◽  
M. Jahangir Alam ◽  
...  
Keyword(s):  
Moisture Content ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Plastic Container ◽  
Level Of Invasion ◽  
Randomized Design ◽  
Two Factors ◽  
Storage Containers ◽  
Wheat Seeds

Abstract A laboratory experiment was conducted at the Department of Agronomy, Hajee Mohammad Danesh Science and Technology University, Dianjpur-5200, Bangladesh to evaluate the storage containers and duration of seed storage on the germinability and health of wheat seeds. The experiment was carried out in two factors, viz. three storage containers naming i) sealed tin container, ii) plastic container, iii) gunny bag, and four storage periods of i) 15 days, ii) 30 days, iii) 45 days, and iv) 60 days. Completely randomized design (CRD) was used in this experiment with eight replications. The results revealed that the germination percentages (GP) of the seeds stored in the gunny bag decreased quickly from 66.1 to 32.8% due to contained with high moisture content in seed. But, slowly decreasing trends of GP from 80.4% to 69.2% was observed in the sealed tin container seeds with lesser moisture content than that of gunny bag and plastic container. The reduction of GP was so higher of 50.38% in the seeds contained in gunny bag than that of only 13.93% in the seeds contained sealed tin container. Wheat seeds stored in sealed tin container, plastic container and gunny bag significantly increased moisture content in ambient condition for 60 DAS. The moisture content of the seeds stored in gunny bag was found to rise remarkably more than other containers. This escalation of seed moisture content was closely related to the surrounding environmental conditions, like temperature and relative humidity where seeds were stored. The rate seed deterioration in gunny bag and plastic container paralleled the level of invasion by storage insect was found. During storage period, insect infected the seeds, and the insect bitten seeds were also found higher in gunny bag and plastic container, but lower in sealed container. Wheat seeds should be stored in air tight sealed container and drying should be done after some days of storage (45-60 DAS).

scholarly journals Model Dinamik Vigor Daya Simpan Benih Kedelai pada Penyimpanan Terbuka

2016 ◽  
Vol 34 (3) ◽  
pp. 219
Author(s):  
Ari Wahyuni ◽  
M. R. Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Initial Moisture Content ◽  
Seed Viability ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Input Model ◽  
Seed Storability

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>

scholarly journals Effect of storage containers and time on seed quality of wheat

1970 ◽  
Vol 33 (3) ◽  
pp. 469-477 ◽  
Author(s):  
PK Malaker ◽  
IH Mian ◽  
KA Bhuiyan ◽  
AM Akanda ◽  
MMA Reza
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Seed Quality ◽  
Storage Period ◽  
Bipolaris Sorokiniana ◽  
Monthly Interval ◽  
Curvularia Lunata ◽  
Seed Moisture Content ◽  
Black Point ◽  
Wheat Seeds

A study was undertaken to determine the prevalence of black point and percentages of germination, moisture content and different fungi associated with wheat seeds during storage in different types of container. Seeds of a widely cultivated variety Kanchan were stored in five types of containers viz., ‘dole'(bamboo made), earthen pitcher, tin container, polyethylene bag and refrigerator (10°C) for ten months at room temperature. Samples were taken at monthly interval beginning from the month of April (prior to storage). The seed moisture content and black point severity were found highest in dole resulting in the lowest percentage of seed germination. The highest germination percentage was observed under storage in refrigerator followed by polyethylene bag, tin container and earthen pitcher. The moisture content and black point infection increased and seed germination decreased with the increase of storage period. Prior to storage in April, the seed germination was 95%, which decreased to about 75% at the end often months of storage (in January). Various fungal flora associated with wheat seeds differed in their prevalence depending on the length of storage period and types of container used for storage. The population of field fungi viz., Alternaria alternata, Aiternaria triticina, Bipolaris sorokiniana, Cladosporium cladosporioides, Curvularia lunata, Epicoccum purpurascens and Fusarium spp. decreased while that of storage fungi viz., Aspergillus, Chaetomium, Nigrospora, Penicillium and Rhizopus increased with the progress of storage period. Key Words: Storage, seed quality, wheat. doi:10.3329/bjar.v33i3.1606 Bangladesh J. Agril. Res. 33(3) : 469-477, September 2008

scholarly journals VIABILITY OF OAT AND BARLEY SEEDS DURING STORAGE

2018 ◽  
Vol 48 (3) ◽  
pp. 28-33 ◽  
Author(s):  
A. Ya. Sotnik
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Initial Moisture Content ◽  
Storage Period ◽  
Seed Storage ◽  
Glass Bottle ◽  
Seed Moisture Content ◽  
The Third ◽  
Plastic Bottle ◽  
Standard Paper

The work presents the evaluation results of the viability of oat and barley collection varieties during storage in the laboratory conditions at the temperature of 20-25o C. Seed storage covered the period of 2006-2017. The research included 24 varieties of barley and 29 varieties of chaffy oats of domestic and foreign breeding collection. The seeds were stored in different packaging materials such as standard paper package; glass bottles of 0.25 liter and plastic bottles of 0.5 liter. The initial seed moisture content was 8.5-9.0 per cent. The varieties were divided into groups taking into account the initial laboratory seed germinating ability. For oats the fi rst group included 10 varieties with initial germination of 96-99%; the second group included 12 varieties with initial germination of 91-95%; the third group included 7 varieties with initial germination of 86-90%. For barley the fi rst group consisted of 5 varieties with initial germination of 91-99%; the second group consisted of 10 varieties with initial germination of 81-90%; the third group consisted of 9 varieties with initial germination of 71-80%. Determination of seed quality was carried out in 7 and 11 years’ time. The initial moisture content of seeds remained without signifi cant changes in all packaging types during the storage period of 11 years. It was also noted that germination of oat and barley seeds during 7 years of storage did not differ in any type of package. The average value in all three groups of oats was 93.2%. After 11 years of storage, germination of oat differed a little, depending on the type of package. In a paper bag it was 80.4%; in a glass bottle – 88.2%; in a plastic bottle – 85.1%. The average initial germination of three groups of barley in 7 years’ time of storage was 84.7%. After 11 years of storage, germination of barley was 53.2% in a paper bag; 63.3% in a glass bottle; 61.8% in a plastic bottle It was revealed that oat and barley seed germination in 7 years’ time did not differ in any type of package. After 11 years, germination of oats was 5.8% higher, and germination of barley was 8.6% higher in a sealed plastic bottle than in a standard paper bag. Differences in seed germinating ability between the glass and plastic bottles were not observed.

scholarly journals Pola Penurunan Viabilitas dan Pengembangan Metode Pendugaan Vigor Daya Simpan Benih Pepaya (Carica Papaya L.)

2017 ◽  
Vol 7 (3) ◽  
pp. 146
Author(s):  
Astryani Rosyad ◽  
M. Rahmad Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Aging Time ◽  
Seed Quality ◽  
Initial Moisture Content ◽  
Accelerated Aging ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Vigor ◽  
Develop Model ◽  
Papaya Seed

<p>ABSTRACT<br />Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method<br />between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research<br />was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage.<br />Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulation</p><p>ABSTRAK<br />Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode<br />pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian<br />Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual.<br />Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih,<br />simulasi</p>

scholarly journals Physiological quality of pink pepper seeds (Schinus terebinthifolia Raddi.) as a function of storage

2021 ◽  
Vol 38 (1) ◽  
pp. 144-157
Author(s):  
Thaísa De Oliveira Silveira ◽  
Madelon Rodrigues Braz ◽  
Gilmara Pires de Moura Palermo ◽  
Tiago Böer Breier
Keyword(s):  
Moisture Content ◽  
Storage Time ◽  
Storage Period ◽  
Seed Storage ◽  
Dry Weight ◽  
Native Forest ◽  
Alternative Source ◽  
Physiological Quality ◽  
And Storage

Seeds of native forest species for food and seedling production have a growing demand that impacts the need for studies involving seed storage in order to maintain good levels of germination quality. Thus, scientific analyzes that explore the physiological potential of the seeds of these species are necessary, especially for the pink pepper (Schinus terebinthifolia Raddi.), a species used as an alternative source of income for traditional communities and which has been arousing interest due to the consumption of its fruits as a condiment in the national and international market. This work aimed to assess the physiological quality of pink pepper seeds according to the storage period and seed size. The seeds came from a rural settlement, a pioneer in the extraction of pink pepper, in the state of Rio de Janeiro, Brazil. The seeds were evaluated for moisture content, germination, first count, length and dry weight of the germinated seedlings, during the period of time that they remained stored and the size of the seeds. The results allowed to conclude that: i) the germinative percentage of the stored pink pepper seeds decreased over the months; ii) the moisture content of the seeds decreased over the months evaluated iii) the different sizes of seeds did not show differences in germination and storage time and iv) values of length and dry weight of the seedlings did not differ according to the period of storage.

Seed storage behaviour of 101 woody species from the tropical rainforest of southern China: a test of the seed-coat ratio–seed mass (SCR–SM) model for determination of desiccation sensitivity

2014 ◽  
Vol 62 (4) ◽  
pp. 305 ◽  
Author(s):  
Qin-ying Lan ◽  
Ke Xia ◽  
Xiao-feng Wang ◽  
Jun-wei Liu ◽  
Jin Zhao ◽  
...  
Keyword(s):  
Moisture Content ◽  
Seed Coat ◽  
Tropical Rainforest ◽  
Southern China ◽  
Seed Mass ◽  
Seed Storage ◽  
Woody Species ◽  
Seed Moisture Content ◽  
Seed Desiccation ◽  
Desiccation Sensitivity

The Xishuangbanna tropical rainforest in Yunnan Province is the greatest biodiversity hotspot in China. However, the biodiversity of this region is under threat, making seed conservation through seed and/or germplasm banking particularly urgent and crucial. Seed desiccation sensitivity limits the possibility of seed banking of 47% of tropical rainforest species. Thus, knowing if a species has desiccation-sensitive seeds is an important first step in seed banking; however, often resources are limited, making it difficult to determine storage behaviour for all the species in a region. Prediction of seed sensitivity using the SCR–SM model based on seed-coat ratio (SCR) and seed dry mass (SM) might be an alternative for determining desiccation sensitivity of seeds of each species. Here, seed-desiccation sensitivity of 101 woody species from the Xishuangbanna tropical forest were analysed using this model, and physiological determinations were made for a total of 25 species. Seed storage behaviour for 59 species was used for model validation, and storage behaviour of 88% of these species was successfully predicted. Seed storage behaviour of 83% of the 59 species was successfully predicted using the 1000-seed weigth–moisture content (TSW–MC) criteria, which include seeds with 1000-seed weight >500 g and seed moisture content at shedding of 30 –70%. The two predictive methods were subsequently used to predict seed desiccation sensitivity for another 42 species from Xishuangbanna whose storage behaviour was uncertain. Our results indicated that ~50% of the species in Xishuangbanna are likely to have desiccation-sensitive seeds.

Reimposition of conditional dormancy during air-dry storage of prechilled Sitka spruce seeds

1998 ◽  
Vol 8 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Steve K. Jones ◽  
Peter G. Gosling ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Water Potential ◽  
Seed Storage ◽  
Sitka Spruce ◽  
Picea Sitchensis ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Dry Storage ◽  
Low Water Potential ◽  
Negative Exponential

AbstractPrechilling seeds of Sitka spruce (Picea sitchensis[Bong.] Carr.) at 4°C with 30% moisture content for 12–14 weeks (84–98 d) removed conditional dormancy (i.e. they were then able to germinate at 10°C). The non-dormant status was preserved after redrying to 6% moisture content. However, conditional dormancy was gradually reimposed during subsequent air-dry storage at 4°C and 6% seed moisture content in all five seed lots tested. Further investigations with one seed lot showed that reimposition was reversed by a second prechill treatment, but was reimposed again during subsequent air-dry storage. The trend of dormancy reimposition within seed lots over time was quantified by negative exponential relations between ability to germinate at 10°C and duration of air-dry storage. The progress of dormancy reimposition was influenced by seed storage moisture content and was most rapid at 4–10%. At higher moisture contents (15 and 20%) the rate of the reimposition of conditional dormancy was much reduced, while at moisture contents of 25 and 30% further loss in dormancy occurred. Thus it is clear that dormancy reimposition occurred during storage at low water potential rather than solely during desiccation from high to low water potential.

scholarly journals Pengaruh Konsentrasi Asap Cair Bambu Tabah (Gigantochloa nigrociliata Buse-Kurz) dan Lama Perendaman Terhadap Kesegaran Pisang Cavendish (Musa Acuminata)

2021 ◽  
Vol 9 (1) ◽  
pp. 45
Author(s):  
K Justikha Natalia Praja ◽  
Pande Ketut Diah Kencana ◽  
I Gusti Ketut Arya Arthawan
Keyword(s):  
Weight Loss ◽  
Storage Temperature ◽  
Storage Period ◽  
Soaking Time ◽  
Average Value ◽  
Liquid Smoke ◽  
Randomized Design ◽  
Two Factors ◽  
Four Levels ◽  
Natural Preservatives

ABSTRAK Buah pisang Cavendish merupakan salah satu produk hortikultura yang rentan mengalami kemunduran fisiologis dan sangat rentan terhadap kerusakan fisik setelah panen. Penggunaan berbagai konsentrasi asap cair bambu tabah dengan lama perendaman yang berbeda dapat digunakan sebagai pengawet alami. Buah pisang yang digunakan adalah pisang Cavendish yang berasal dari Kabupaten Buleleng, dalam satu sisir berisi paling sedikit10 buah dengan panjang rata-rata 18.89 cm dan berat rata-rata 125  gram (g). Tujuan penelitian ini adalah menemukan kombinasi perlakuan terbaik dari perlakuan konsentrasi asap cair dan lama perendaman untuk menjaga kesegaran dan masa simpan pada buah pisang Cavendish selama penyimpanan. Rancangan percobaan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) faktorial dengan dua faktor, yaitu faktor pertama adalah konsentrasi asap cair bambu tabah dan faktor kedua adalah lama perendaman. Faktor pertama terdiri dari empat taraf konsentrasi yaitu 1%, 3%, 5%, dan 7%. Faktor kedua terdiri dari tiga taraf lama perendaman yaitu 10 menit, 20 menit dan 30 menit dengan suhu penyimpanan menggunakan suhu ruang (28±2oC).  Parameter yang diamati dalam penelitian ini adalah laju respirasi, susut bobot, kekerasan, TPT, tingkat kerusakan, dan organoleptik. Hasil interaksi terbaik ditunjukkan pada perlakuan 7% konsentrasi asap cair bambu tabah dan perendaman 10 menit, dengan nilai rata-rata parameter laju respirasi 51.18 ml CO2/kg.jam, nilai rata-rata susut bobot dengan nilai 17.61%, nilai rata-rata kekerasan 3.82 kg, nilai rata-rata TPT 15.65 %Brix, nilai tingkat kerusakan 2.40, nilai rata-rata organoleptik rasa sebesar 4.67, aroma sebesar 4.54 dan warna sebesar 3.87. Disimpulkan bahwa kombinasi perlakuan A4B1 (konsentrasi asap cair bambu tabah 7% dengan perendaman selama 10 menit) adalah kombinasi terbaik untuk menjaga kesegaran dan memperlambat penurunan kualitas buah pisang Cavendish hingga akhir periode penyimpanan hari ke-15. ABSTRACT Cavendish banana one of the horticultural products that are prone to physiological setbacks and very vulnerable to physical damages after harvest. Various concentrations of tabah bamboo liquid smoke with different soaking times can use as natural preservatives. The bananas used in this study were Cavendish bananas from Buleleng, every bunch consists of at least ten banana with a length of 18.59 cm and a weight of 125 ± 5 g. This study aims to find the best treatment combination of the treatmentoof liquid smoke concentration and soaking time to maintain freshness and shelf life of Cavendish bananas during storage. The experimental design used in this study was a Completely Randomized Design (CRD) with two factors, the first factor was the concentration of tabah bamboo liquid smoke, and the second factor was the soaking time. The first factor consists of four levels of concentration namely 1%, 3%, 5%, and 7%. The second factor consists of three levels of soaking time, namely 10 minutes, 20 minutes and 30 minutes with storage temperature using room temperature (28 ± 2oC).  The parameters observed in this study are: repiration rate, weight loss, hardness, level of damages, TPT and organoleptics. The best interaction results are shown in the treatment of 7% concentration of tabah bamboo liquid smoke with 10 minutes of soaking with an average value of respiration rate parameters of 51.18 ml CO2 / kg.hour, an average value of weight loss with a value of 17.61%, an average value of hardness of 3.82 kg, the average value of TPT was 15.65% Brix, the value of the level of damage was 2.40, the average organoleptic value of taste was 4.67, aroma was 4.54 and color was 3.87. The conclus of this study indicates that the combination of A4B1 (7% tabah bamboo liquid smoke concentration with soaking for 10 minutes) is the best combination to keep freshness and the quality of green bananas until the storage period of the 15th day.

scholarly journals Effects of different post-harvest treatments on bio-chemical characters and diseases of litchi in storage

2020 ◽  
Vol 8 (1) ◽  
pp. 57
Author(s):  
Fakhar Uddin Talukder ◽  
Md. Sohanur Rahman ◽  
Md. Kamrul Hassan
Keyword(s):  
Low Temperature ◽  
Vitamin C ◽  
Ambient Temperature ◽  
Disease Incidence ◽  
Storage Period ◽  
Post Harvest ◽  
Randomized Design ◽  
Two Factors ◽  
Soluble Solid ◽  
Chemical Characters

Objective of the study was to evaluate the effects of different post-harvest treatments on bio-chemical characters and diseases of litchi (Litchi chinensis Sonn, var. Bombai). The experiment consisted of two factors. Factor A: Temperature viz. T1: Ambient temperature, T2: 4ºC temperature; Factor B: PP bags (Polypropylene bag) viz. P1: Control (unwrapped), P2: 50 micro meter (µm) PP bag, P3: 75µmm PP bag, P4: 100µm PP bag. The experiment was conducted in completely randomized design (CRD) with three replications. Significant variation was observed in total soluble solid, PH of fruit pulp, vitamin c content, percent disease incidence and disease severity during the storage period. TSS contents increased up to the 6th day of storage and there after declined. pH values were maximum (4.14) in the fruits kept in 100µ polypropylene bag at ambient temperature. Vitamin C continent decreased with the increase of storage period. Disease incidence and severity progress with the storage period. Among the treated and untreated fruits, 75µm pp bag at low temperature (4ºC) treatment exhibited better storage performance. More research should be conducted by using other litchi cultivar like Bedana, China-3 etc. Various technologies have been devised to minimize the post-harvest losses of litchi, one of such technologies is the use of PP bag & low temperature.  

scholarly journals Aplikasi Bakteri Probiotik untuk Meningkatkan Mutu Fisiologi dan Kesehatan Bibit Cabai (Capsicum annuum L.)

2016 ◽  
Vol 44 (2) ◽  
pp. 176 ◽  
Author(s):  
Anna Tefa ◽  
Eny Widajati ◽  
Muhamad Syukur ◽  
Dan Giyanto
Keyword(s):  
Storage Period ◽  
Seed Storage ◽  
Colletotrichum Acutatum ◽  
Seed Coating ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Seedling Vigour ◽  
Chilli Pepper ◽  
Control Seed ◽  
Randomized Design

ABSTRACT<br /><br />The use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period.<br /><br />Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period

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