scholarly journals Lacticaseibacilli and Lactococci from Slovak Raw Goat Milk and their Potential

2021 ◽  
Vol 52 (2) ◽  
pp. 19-28
Author(s):  
A. Lauková ◽  
M. Pogány Simonová ◽  
M. Tomáška ◽  
M. Kološta ◽  
M. Drončovský ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Enzyme Production ◽  
Skim Milk ◽  
Goat Milk ◽  
Inhibition Zone ◽  
Low Ph ◽  
Low Grade ◽  
Inhibition Activity ◽  
Bacteriocin Activity ◽  
Additional Testing

Abstract Strains potential such as bacteriocin activity, biofilm formation ability, growth in skim milk, susceptibility to antibiotics, tolerance to bile and low pH as well as enzyme production was tested in the species Lacticaseibacillus paracasei and Lactococcus lactis detected in Slovak raw goat milk. The strains showed mostly low-grade biofilm formation ability, susceptibility to antibiotics and sufficient tolerance to oxgall/bile. Lacticaseibacillus (Lcb.) paracasei ZM-1, ZM-2 and Lactococcus (Lc.) lactis PD MO 1/8 showed high tolerance to pH 3 (67 %, 83 % and 63 %, respectively). The strains showed bacteriocin activity against the principal indicator Enterococcus avium EA5 (inhibition zone ranging 5–24 mm). A concentrated substance of Lcb. paracasei LPa ML 12/1 (pH 6.3) inhibited EA5 strain (inhibition activity 100 AU ml–1). Lcb. paracasei ZM-1 and ZM-2 also produced a high amount of β-galactosidase (40 nmol). Although the strains indicated their beneficial potential, additional testing is needed; some tests are in processing for further possible application of selected strains in dairy.

scholarly journals Susceptibility to Bacteriocins in Biofilm-Forming, Variable Staphylococci Isolated from Local Slovak Ewes’ Milk Lump Cheeses

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1335 ◽  
Author(s):  
Andrea Lauková ◽  
Monika Pogány Simonová ◽  
Valentína Focková ◽  
Miroslav Kološta ◽  
Martin Tomáška ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Basic Research ◽  
Inhibition Zone ◽  
Low Grade ◽  
Inhibition Activity ◽  
Treatment Benefit ◽  
Staphylococcus Equorum ◽  
Ewe's Milk ◽  
Staphylococcus Simulans ◽  
High Inhibition

Seventeen staphylococci isolated from 54 Slovak local lump cheeses made from ewes’ milk were taxonomically allotted to five species and three clusters/groups involving the following species: Staphylococcus aureus (5 strains), Staphylococcus xylosus (3 strains), Staphylococcus equorum (one strain) Staphylococcus succinus (5 strains) and Staphylococcus simulans (3 strains). Five different species were determined. The aim of the study follows two lines: basic research in connection with staphylococci, and further possible application of the bacteriocins. Identified staphylococci were mostly susceptible to antibiotics (10 out of 14 antibiotics). Strains showed γ-hemolysis (meaning they did not form hemolysis) except for S. aureus SAOS1/1 strain, which formed β-hemolysis. S. aureus SAOS1/1 strain was also DNase positive as did S. aureus SAOS5/2 and SAOS51/3. The other staphylococci were DNase negative. S. aureus SAOS1/1 and SAOS51/3 showed biofilm formation on Congo red agar. However, using quantitative plate assay, 12 strains out of 17 showed low-grade biofilm formation (0.1 ≤ A570 < 1), while five strains did not form biofilm (A570 < 0.1). The growth of all strains, including those strains resistant to enterocins, was inhibited by nisin and gallidermin, with high inhibition activity resulting in the inhibition zone in size from 1600 up to 102,400 AU/mL (arbitrary unit per milliliter). This study contributes to microbiota colonization associated with raw ewe’s milk lump cheeses; it also indicates bacteriocin treatment benefit, which can be used in prevention and/or elimination of staphylococci.

scholarly journals Enterococcal Species Associated with Slovak Raw Goat Milk, Their Safety and Susceptibility to Lantibiotics and Durancin ED26E/7

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 681
Author(s):  
Andrea Lauková ◽  
Valentína Focková ◽  
Monika Pogány Pogány Simonová
Keyword(s):  
Biofilm Formation ◽  
Goat Milk ◽  
Surface Protein ◽  
Human Consumption ◽  
Low Grade ◽  
Pathogenic Potential ◽  
Aggregation Substance ◽  
Enterococcal Species ◽  
Traditional Dairy Products ◽  
Original Information

Goat milk has become a popular item of human consumption due to its originality. Enterococci are ubiquitous bacteria, and they can also be found in traditional dairy products. This study focuses on the safety of enterococci from Slovak raw goat milk and on their susceptibility to lantibiotic bacteriocins and durancin ED26E/7, which has not previously been studied. Biofilm formation ability in enterococci, virulence factor genes, enzyme production and antibiotic profile were investigated. Samples of raw goat milk (53) were collected from 283 goats in Slovakia. MALDI-TOF mass spectrometry identified three enterococcal species: Enterococcus faecium, E. hirae and E. mundtii, with dominant occurrence of the species E. faecium. Low-grade biofilm formation ability (0.1 ≤ A570 < 1.0) was found in four strains of E. faecium.Gelatinase, hyaluronidase, aggregation substance and enterococcal surface protein genes were absent in these enterococci. Gene efaAfm (adhesin) was detected in five E. faecium strains. However, it was not detected in biofilm-forming strains. Enterococci detected in Slovak raw goat milk were found not to have pathogenic potential; four strains even produced high amounts of useful β-galactosidase. The strains were susceptible to lantibiotic bacteriocin treatment and to durancin ED26E/7 as well, which represents original information in dairy production.

scholarly journals Enterococcus mundtii Isolated from Slovak Raw Goat Milk and Its Bacteriocinogenic Potential

2020 ◽  
Vol 17 (24) ◽  
pp. 9504
Author(s):  
Andrea Lauková ◽  
Valentína Focková ◽  
Monika Pogány Simonová
Keyword(s):  
Food System ◽  
Skim Milk ◽  
Goat Milk ◽  
Acid Tolerance ◽  
Storage Conditions ◽  
Indicator Strain ◽  
No Production ◽  
Inhibition Activity ◽  
C Storage ◽  
Enterococcus Mundtii

Enterococci are lactic acid bacteria. Most of them can adapt well to the food system due to their salt and acid-tolerance. Moreover, many enterococcal species have been found to produce antimicrobial substances of proteinaceous character, i.e., bacteriocins/enterocins. In this study, Enterococcus mundtii EM ML2/2 with bacteriocinogenic potential was identified in Slovak raw goat milk. This strain demonstrated inhibition activity against up to 36% of Gram-positive indicator bacteria, and in concentrated form the bacteriocin substance (pH 6.3) showed the highest inhibition activity (1600 AU/mL) against the principal indicator strain E. avium EA5. Semi-purified substance (SPS) EM ML2/2 produced inhibition activity up to 3200 AU/mL. Concentrated bacteriocin substance and SPS maintained active (inhibition activity up to 100 AU/mL) for three months under −20 °C storage conditions. The strain showed susceptible antibiotic profile, and it did not form biofilm. No production of damaging enzymes was noted. It was nonhemolytic, as well as DNase, and gelatinase-negative. It grew well in skim milk, and it was salt and acid-tolerant. The bacteriocin potential of E. mundtii species isolated from Slovak raw goat milk has not previously been detected, so this is an original contribution which may stimulate addtitional research and application studies.

scholarly journals Susceptibility to Enterocins and Lantibiotic Bacteriocins of Biofilm-Forming Enterococci Isolated from Slovak Fermented Meat Products Available on the Market

2020 ◽  
Vol 17 (24) ◽  
pp. 9586
Author(s):  
Andrea Lauková ◽  
Anna Kandričáková ◽  
Eva Bino
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Food Industry ◽  
Meat Products ◽  
Low Grade ◽  
Enterococcus Hirae ◽  
Conventional Antibiotics ◽  
Enterocin A

This study investigated eight types of Slovak dry fermented meat products (salami and sausages) that are available on the market and were produced by three different producers in different regions of Slovakia. The total counts of enterococci in these products ranged from 2.0 up to 6.0 cfu/g (log10). Three species were identified among the 15 selected enterococcal strains; Enterococcus faecium (8 strains), Enterococcus faecalis (3) and Enterococcus hirae (4). They were hemolysis-negative (γ-hemolysis) with a biofilm-forming ability, which was evaluated as low-grade biofilm formation, susceptible to conventional antibiotics and mainly susceptible to lantibiotic bacteriocins, namely, gallidermin and nisin; they even showed a higher susceptibility to gallidermin than to nisin. They were also susceptible to enterocin–durancin, but most strains showed resistance to enterocin A/P. This study indicated that bacteriocins can play a key role in preventing and/or protecting from undesirable bacterial multiplication or contamination in the food industry and that they have great potential for further experimental applications.

scholarly journals Chemical sanitizers to control biofilms formed by two Pseudomonas species on stainless steel surface

2012 ◽  
Vol 32 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Danila Soares Caixeta ◽  
Thiago Henrique Scarpa ◽  
Danilo Florisvaldo Brugnera ◽  
Dieyckson Osvani Freire ◽  
Eduardo Alves ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stainless Steel ◽  
Biofilm Formation ◽  
Skim Milk ◽  
304 Stainless Steel ◽  
Stainless Steel Surface ◽  
Aisi 304 ◽  
Pseudomonas Species ◽  
Sodium Dichloroisocyanurate ◽  
Different Temperatures

The biofilm formation of Pseudomonas aeruginosa and Pseudomonas fluorescens on AISI 304 stainless steel in the presence of reconstituted skim milk under different temperatures was conducted, and the potential of three chemical sanitizers in removing the mono-species biofilms formed was compared. Pseudomonas aeruginosa cultivated in skim milk at 28 °C presented better growth rate (10.4 log CFU.mL-1) when compared with 3.7 and 4.2 log CFU.mL-1 for P. aeruginosa and P. fluorescens cultivated at 7 °C, respectively. Pseudomonas aeruginosa formed biofilm when cultivated at 28 °C. However, only the adhesion of P. aeruginosa and P. fluorescens was observed when incubated at 7 °C. The sodium dichloroisocyanurate was the most efficient sanitizer in the reduction of the adhered P. aeruginosa cells at 7 and 28 °C and those on the biofilm, respectively. The hydrogen peroxide was more effective in the reduction of adhered cells of P. fluorescens at 7 °C.

scholarly journals Streptococcus mutans Lacking sufCDSUB Is Viable, but Displays Major Defects in Growth, Stress Tolerance Responses and Biofilm Formation

2021 ◽  
Vol 12 ◽  
Author(s):  
Kassapa Ellepola ◽  
Xiaochang Huang ◽  
Ryan P. Riley ◽  
Jacob P. Bitoun ◽  
Zezhang Tom Wen
Keyword(s):  
Stress Tolerance ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Nitrosative Stress ◽  
Growth Stress ◽  
Low Ph ◽  
Luciferase Reporter ◽  
Deficient Mutant ◽  
Oxidative And Nitrosative Stress ◽  
Isoleucine Leucine

Streptococcus mutans appears to possess a sole iron-sulfur (Fe-S) cluster biosynthesis system encoded by the sufCDSUB cluster. This study was designed to examine the role of sufCDSUB in S. mutans physiology. Allelic exchange mutants deficient of the whole sufCDSUB cluster and in individual genes were constructed. Compared to the wild-type, UA159, the sufCDSUB-deficient mutant, Δsuf::kanr, had a significantly reduced growth rate, especially in medium with the absence of isoleucine, leucine or glutamate/glutamine, amino acids that require Fe-S clusters for biosynthesis and when grown with medium adjusted to pH 6.0 and under oxidative and nitrosative stress conditions. Relative to UA159, Δsuf::kanr had major defects in stress tolerance responses with reduced survival rate of &gt; 2-logs following incubation at low pH environment or after hydrogen peroxide challenge. When compared to UA159, Δsuf::kanr tended to form aggregates in broth medium and accumulated significantly less biofilm. As shown by luciferase reporter fusion assays, the expression of sufCDSUB was elevated by &gt; 5.4-fold when the reporter strain was transferred from iron sufficient medium to iron-limiting medium. Oxidative stress induced by methyl viologen increased sufCDSUB expression by &gt; 2-fold, and incubation in a low pH environment led to reduction of sufCDSUB expression by &gt; 7-fold. These results suggest that lacking of SufCDSUB in S. mutans causes major defects in various cellular processes of the deficient mutant, including growth, stress tolerance responses and biofilm formation. In addition, the viability of the deficient mutant also suggests that SUF, the sole Fe-S cluster machinery identified is non-essential in S. mutans, which is not known in any other bacterium lacking the NIF and/or ISC system. However, how the bacterium compensates the Fe-S deficiency and if any novel Fe-S assembly systems exist in this bacterium await further investigation.

scholarly journals Efektivitas Infusa Daun Talok (Muntingia calabura) dalam Menghambat Pertumbuhan Bakteri Klebsiella pneumoniae

2019 ◽  
Vol 2 (3) ◽  
pp. 223
Author(s):  
Fitri Nadifah ◽  
Desto Arisandi ◽  
Siti Fatimah ◽  
Dani Setiawan
Keyword(s):  
Experimental Study ◽  
Urinary Tract Infection ◽  
Klebsiella Pneumoniae ◽  
Inhibition Zone ◽  
Inhibition Activity ◽  
Strong Activity ◽  
Research Goal ◽  
Agar Method ◽  
Water Treatments ◽  
Muntingia Calabura

Klebsiella pneumoniae is one of the most bacteria found in urine of Urinary Tract Infection (UTI) Patients. Study in many countries has found that this species become resistant to more than one antibiotics. This research goal is to know the effectiveness of talok (Muntingia calabura) infused water inhibiting the growth of K. pneumoniae. This was an experimental study using K. pneumoniae bacteria isolated from urine samples using diffuse agar method. The concentration of talok infused water was varied from 25-100% and the antibiotics used was cefotaxime, meropenem and chloramphenicol. The inhibition activity was measured by the diameter of inhibition zone. The results show that K. pneumoniae is still give sensitive response to antibiotic treatments. While the inhibition zone from talok infused water treatments can be categorized in middle and strong activity. From this study we can conclude that talok infused water potential in inhibiting the growth of K. pneumoniae. Future study still needed to develop this potential, especially for the bacteria that has been resistant to some antibiotics.

scholarly journals Occurrence of Enterocin Genes in Enterococci from Slovak Milk Product Žinčica

2019 ◽  
Vol 50 (3) ◽  
pp. 197-202 ◽  
Author(s):  
A. Lauková ◽  
V. Strompfová ◽  
M. Tomáška ◽  
M. Kološta
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Listeria Monocytogenes ◽  
Dairy Product ◽  
Inhibition Zone ◽  
The Other ◽  
Milk Product ◽  
Inhibition Activity ◽  
Gene Detection ◽  
P Gene

Abstract Žinčica is a popular Slovak dairy product made from ewes’ milk. It is a by-product resulting during ewes’ lump cheese processing. Microbiota in Žinčica have rarely been studied, especially enterococci; however, they can produce beneficial substances – bacteriocins. In this study, the presence of Enterocins (Ents) genes were analysed in enterococci from Žinčica and partially also the inhibition activity. Samples of Žinčica were collected from different agrofarms producing ewes’ lump cheese (34) in Central Slovakia. In the enterococci tested, Ent P gene was the most frequently detected (in 6 out of 7 enterococci), followed by Ent A and Ent L50B genes. Ent B gene was detected only in E. faecium 30E1. On the other hand, E. faecalis 31E2 did not contain Ent genes, although it showed inhibition activity against the indicator strains Enterococcus avium EA5, Staphylococcus aureus SA5, Listeria monocytogenes CCM4699 (inhibition zone sizing up to 20 mm). E. faecium 30E1 contained genes of four Ents; however, it showed no inhibition activity. Growth of the four indicators was inhibited due to the antimicrobial activity of E. faecium 32E1 with Ent P gene detection. This is the first study reporting on the occurrence of Ent genes in enterococci from Žinčica.

scholarly journals Prevalence of Tonsillitis caused by Streptococcus spp. Among children and the effect of some Lactic acid bacterial (LAB) strain on it

2016 ◽  
Vol 13 (2) ◽  
pp. 218-227
Author(s):  
Baghdad Science Journal
Keyword(s):  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Bacterial Isolate ◽  
Inhibition Zone ◽  
Laboratory Culture ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Str System

In this study 100 samples were collected from infected children with acute and chronic tonsillitis who attended to Al-Yarmook Teaching Hospital (ENT consultation clinic) from 5/12/2013 to 1/3/2014. The result of laboratory culture was positive in 67 samples. Depending on their cultural, morphological and biochemical characterization of bacterial isolate of them were identified as (37.31%) belonged to Streptococcus pyogenes and the diagnosis is confirmed by the use of Remel Rapid STR System, (34.32%) belonged to S.parasanguinis, (11.94%) S.mitis, (11.94%) S.oralis and (4.47%) S.thoraltensis . Results confirmed that cup assay gave highest inhibition zone after 24 hrs compare with well diffusion methods for suspension of L.acidophilus gave highest inhibition zone after 48 hrs for incubation, while ahigh inhibition zone revealed for suspension of L.fermentum after 24 hrs incubation. the study included also the measurement of the inhibition activity for bacteriocins produced by L.acidophilus bacteria against pathogenic bacteria on nutrient agar by well diffusion method in which results revealed stability of the bacteriocins produced towards PH which kept its activity with PH 4-6 for 24 hrs, and the highest stability was with PH 4, however it lost a lot of its activity with acidic PH less than 2 and alkaline PH as 8. The treatment of bacteriocins with salts such as Nacl it revealed little effect in inhibition zone within 1 & 2% concetrations. The salt MgSo4 & Kcl showed reduction in the inhibitory activity in the low concentration, however the higher concentration of salt caused great reduction and 5% concentration led to loss of inhibitory activity for bacteriocins completely.

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