scholarly journals OPTIMASI METODE EKSTRAKSI KUERSETIN DARI DAUN KELOR (Moringa oleifera L.)

Jurnal Kimia ◽  
2020 ◽  
pp. 19
Author(s):  
N. P. L. Laksmiani ◽  
I W. A. Widiantara ◽  
K. D. Adnyani ◽  
A. B. S. Pawarrangan
Keyword(s):  
Qualitative Analysis ◽  
Extraction Method ◽  
Moringa Oleifera ◽  
Extraction Methods ◽  
Polyphenol Compounds ◽  
Moringa Oleifera Leaves ◽  
Method Optimization

Moringa plants is very abundant in Indonesia. Moringa leaves are a source of polyphenol compounds, namely flavonoids. One of the main flavonoids found in Moringa leaves is quercetin. To be able to increase the concentration of quercetin from moringa plants, extraction is necessary. This study aims to determine the most optimal extraction method in extracting quercetin from Moringa leaves. Optimization of quersein extraction methods from Moringa leaves was carried out using maceration, soxhletation, and reflux methods. The solvent used was methanol acidified with 1,2 N HCl. Determination of the concentration of quercetin in the extract used a validated TLC-densitometry method. The highest rendement obtained from the maceration extraction method was 24.08 % w/w. Extraction by reflux and sochletation methods yielded rendement of 23.44 % w/w and 10.12 % w/w respectively. Qualitative analysis of quercetin in the extract was carried out by comparing its Rf value with the standard quercetin which is 0,4. The quercetin level of the extract from maceration extraction was the highest, which was 24.45 % w/w. Extraction using the soxhletation method obtained quercetin as much as 20.95 % w/w.   Keywords: quercetin, Moringa oleifera leaves, extraction method, optimization

scholarly journals Comparison of Babcock and Ether Extraction Methods for Determination of Fat Content of Cream: Collaborative Study

1996 ◽  
Vol 79 (4) ◽  
pp. 907-916 ◽  
Author(s):  
Joanna M Lynch ◽  
David M Barbano ◽  
J Richard Fleming
Keyword(s):  
Extraction Method ◽  
Collaborative Study ◽  
Extraction Methods ◽  
Fat Content ◽  
Official Method ◽  
Ether Extraction ◽  
Aoac Official ◽  
The Difference ◽  
Aoac Official Method

Abstract A modified Mojonnier ether extraction method for determination of the fat content of cream was developed based on the method for milk (AOAC Official Method 989.05). The cream Babcock method (AOAC Official Method 920.111 B-C) was modified to harmonize with the milk Babcock method (AOAC Official Method 989.04) and to clarify procedural details. Using the AOAC collaborative study format, 10 laboratories tested 9 pairs of blind duplicate heat-treated cream samples with a fat range of 30-45% using both methods. The statistical performance (invalid and outlier data removed) was as follows: mean % fat = 37.932, sr = 0.125, sR = 0.151, RSDr = 0.330, RSDR = 0.398, r = 0.354, and R = 0.427 for the ether extraction method. For the Babcock method, mean % fat = 38.209, sr = 0.209, SR = 0.272, RSDr = 0.548, RSDR = 0.712, r = 0.592, and R = 0.769. Average test results for fat from the Babcock method were 0.277% (absolute fat) greater than for the Mojonnier ether extraction method. The difference between methods, as a percentage of the average fat content of the samples, was 0.73%. This agrees with differences observed between the 2 methods for milk when 10 to 17 laboratories tested 7 milk samples in blind duplicate at bimonthly intervals over a 4-year period (average difference 0.029% fat, 0.78% as a percentage of average fat content). The Mojonnier ether extraction and Babcock methods for fat in cream have been adopted by AOAC INTERNATIONAL. The new Babcock method replaced the AOAC Official Method 920.111 B-C.

Determination of Chemical Composition and Nutritional Values of Moringa oleifera Leaves

2011 ◽  
Vol 10 (3) ◽  
pp. 264-268 ◽  
Author(s):  
Charles Windepagnagd Yameogo ◽  
Marcel Daba Bengaly ◽  
Aly Savadogo ◽  
Philippe Augustin Nikiema ◽  
Sabadenedyo Alfred Traore
Keyword(s):  
Chemical Composition ◽  
Moringa Oleifera ◽  
Nutritional Values ◽  
Moringa Oleifera Leaves

scholarly journals A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves

2021 ◽  
Vol 20 (11) ◽  
pp. 2371-2379
Author(s):  
Yanqin Zhu ◽  
Qinhong Yin ◽  
Yaling Yang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Moringa Oleifera ◽  
Accurate Determination ◽  
Gradient Elution ◽  
Hplc Method ◽  
Array Detector ◽  
Moringa Oleifera Leaves

Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) and high performance liquid chromatography with ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD and UPLC-UV methods were applied for the accurate determination of eleven major polyphenols in Moringa oleifera leaves. The chromatographic conditions of the eleven polyphenols was determined on two C18 column by gradient elution with 0.5 % phosphoric acid solution -acetonitrile as the eluate, and at a flow rate of 1.0 and 0.5 mL/min for HPLC-DAD and UPLC-UV methods, respectively. Detector parameter of UPLC-UV was fixed at 203 nm. The assay methods were validated systematically.Results: The instrumental methods (HPLC-DAD and UPLC-UV) had good linearity, precision,repeatability and recovery. For both methods, quantification limits of UPLC-UV (0.057 - 0.363 μg/mL) were lower than those of UPLC-UV (0.094 - 1.532 μg/mL). The UPLC method with a shorter running time and more sensitive detection was applied in comparing to the HPLC method. After optimization and evaluation, the baseline of 11 compounds was separated effectively within 68 and 34 min, respectively.Conclusion: The developed HPLC-DAD and UPLC-UV assays were successfully utilized for thesimultaneous analysis of eleven major polyphenols and can readily be utilized as quality control tools for Moringa oleifera leaves in China, with UPLC-UV method showing better separation, lower organic solvent usage and shorter analytical period.

Screening for Anti-Diabetic Peptides from Moringa oleifera leaves

2021 ◽  
pp. 5886-5890
Author(s):  
Thiruchelvi. R ◽  
Priyadharshini. S ◽  
Rajakumari K.
Keyword(s):  
Moringa Oleifera ◽  
Protein Extraction ◽  
Extraction Methods ◽  
Clinical Syndrome ◽  
Yeast Cells ◽  
Diabetic Patients ◽  
Small Peptides ◽  
Small Proteins ◽  
Uptake Assay ◽  
Moringa Oleifera Leaves

Moringa oleifera commonly called as Drumstick tree is a multipurpose tree and it is widely present in the places like India, Asia, Africa, etc. Its leaves are emetic and their juices are used for medicinal purposes. Diabetes mellitus is a clinical syndrome with insufficient insulin secretion and abnormal glucose tolerance. It is a group of diseases which results in too much sugar in the blood. Leaves from Moringa oleifera were collected, analysed for their anti- diabetic activity. First the proteins are extracted using protein extraction methods, estimated using Lowry’s method, and then anti- diabetic activity was checked using Glucose uptake assay by yeast cells, where the plant extract (small proteins) was able to uptake glucose. SDS-PAGE was carried out to check the protein’s size and molecular weight. The characterization of Moringa oleifera conducted in this study shows that the small peptides from the leaves of this plant can contribute significantly in the daily recommended intake of it, since it serves as a rich source of all minerals, vitamins etc. This study has confirmed that the small peptides from the leaves of M.oleifera can fight against diabetics and the result indicated that Murungai (Moringa) leaves are suitable source of green leaf to reduce the diabetic complications in diabetic patients.

Comparative Potential Qualitative and Quantitative Phytochemical Evaluation of Neem and Moringa Oleifera Leaf Plants in Ozoro, Delta State, Nigeria

2019 ◽  
pp. 269-274
Author(s):  
Uwague A
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Azadirachta Indica ◽  
Moringa Oleifera ◽  
Active Ingredients ◽  
Leaf Sample ◽  
Standard Methods ◽  
Qualitative And Quantitative ◽  
Moringa Oleifera Leaves

This study was aimed at determining the qualitative and quantitative phytochemicals present in Neem (Azadirachta indica) and Moringa (oleifera) leaves using standard methods. The result of the analysis reveals the phytochemicals as follows; Alkaloid and glycoside were found to be moderately present while flavonoid and steroid were found to be present whereas saponin was not present in the qualitative analysis of the neem (Azadirachta indica) leaf sample while in that of moringa (oleifera), flavonoid, saponin and steroid were found to be moderately present while alkaloid and glycoside were just present. In the quantitative analysis, Neem (Azadirachta indica) was found to contain 4.00% of saponin while that of moringa (oleifera) was 19.0%, flavonoid was found to contain 2.10% in neem while that of moringa was 11.2%, alkaloid was found to contain 14.5% in neem while that of moringa was 8.00%, glycoside was found to contain 0.27% in neem while that of moringa was 0.18%, steroid was found to contain 0.03% in neem while moringa was found to contain 0.12%. The phytochemicals are present but occurs in different degrees, hence further research and identification of these active ingredients should be carried out through various techniques.

Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves

2019 ◽  
Vol 115 ◽  
pp. 319-327 ◽  
Author(s):  
Gabriele Rocchetti ◽  
Francesca Blasi ◽  
Domenico Montesano ◽  
Silvia Ghisoni ◽  
Maria Carla Marcotullio ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Extraction Methods ◽  
Phenolic Profile ◽  
Moringa Oleifera Leaves ◽  
Conventional Extraction

scholarly journals Study on the Effect of Different Extraction Methods in Four Pharmacopoeia on the Content and Structural Transformation of Ginsenoside and the Optimization of Extraction Process

2021 ◽  
Author(s):  
Kai Sun ◽  
Hua Jiang ◽  
Pingya Li ◽  
Lei Xu ◽  
Yaling Deng ◽  
...  
Keyword(s):  
United States ◽  
Structural Transformation ◽  
Extraction Method ◽  
The United States ◽  
Extraction Process ◽  
Extraction Methods ◽  
Detection Methods ◽  
Content Determination ◽  
Q Exactive

Abstract Background: In recent years, ginseng products are widely used in various fields. More and more people pay attention to the extraction methods and quality evaluation of ginseng. At present, China, the United States, Europe, Japan and Korea have the quality standards and content determination methods of ginseng. However, due to the different treatment methods adopted before the determination of ginseng samples, the content limits of the index components, such as ginsenoside Rb1, Rg1 and Re are also different. There have been literature analyzed the similarities and differences of ginseng content detection methods in pharmacopoeias of different countries, but the comparison of the effects of different methods on ginsenoside content and structural transformation has not been reported.Methods: In this paper, ginsenosides in ginseng were extracted according to four national Pharmacopoeia, and analyzed quantitatively and qualitatively by UPLC-Q-Exactive-MS and HPLC-UV. Finally, a simple and feasible extraction method was optimized by response surface method. Results: Twelve kinds of ginsenosides in ginseng were quantitatively analyzed by using the methods of four national pharmacopoeia. Among them, the contents of Rg1, Re and Rd were high, and they were the highest by using unheated J/KP (Japan/Korea Pharmacopoeia) method. Ten kinds of ginsenosides were determined by heated CP (China Pharmacopoeia), USP (the United States Pharmacopoeia) and EP (European Pharmacopoeia) method, and seven kinds of ginsenosides were determined by unheated J/KP method. In the following UPLC-Q-Exactive-MS study, 34, 36, 21 and 19 ginsenosides were identified by CP, USP, EP and J/KP method, respectively. In the optimization of ginsenoside extraction process, an efficient extraction method was selected from the solvent, extraction time, solid-liquid ratio and other factors. In conclusion, through the qualitative and quantitative comparison of CP, USP and EP samples after heating, it can be seen that ginsenoside heating will increase the content of rare saponins, and the heating time is directly proportional to the content of rare saponins. Conclusion: The pretreatment method has a significant effect on the content determination of ginseng. The analysis of the preparation method and process optimization of the four Pharmacopoeia can provide important reference for the revision of ginseng standard.

scholarly journals Determination of Suitable Extraction Method for the Available Iron (Fe) Content of Calcareous Soils

2018 ◽  
Vol 6 (1) ◽  
pp. 51
Author(s):  
Aydın Adiloğlu ◽  
Sevinç Adiloğlu ◽  
Mehmet Rüştü Karaman
Keyword(s):  
Extraction Method ◽  
Calcareous Soils ◽  
Correlation Coefficients ◽  
Triticum Aestivum L ◽  
Extraction Methods ◽  
Research Area ◽  
Biological Indices ◽  
Fe Content ◽  
Plant Grown

The aim of this research was to determine the most suitable extraction method for the available iron contents of calcareous soils in Trakya Region, Turkey. For this purpose ten calcareous soil samples were taken from research area and five extraction methods (Lindsay and Norvell, Wear and Evans, Olson, Soltanpur and Mehlich methods) were used and three biological indices (dry matter yield, Fe concentration, Fe uptake) were compared. The plant biological indices were determined with wheat (Triticum aestivum L.) plant grown under greenhouse conditions. At the end of the experiment, the highest correlation coefficients (r) were determined between the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA, 0.005 M DTPA + 1 M NH4HCO3 methods and the biological indices. The correlation coefficients (r) for the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA method and the three biological indices were 0.648**, 0.780** and 0.656** respectively. For the 0.005 M DTPA + 1 M NH4HCO3 method, these coefficients were determined 0.595**, 0.637** and 0.625**, respectively. Consequently, these extraction methods were suggested for the determination of the available Fe contents of the calcareous soils in Trakya Region, Turkey.

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