scholarly journals Jersey İneklerinde STAT5A Geninin Süt Verimi ve Bileşenleri Üzerine Etkisinin Araştırılması

Author(s):  
Soner Çankaya ◽  
Mehmet Ülker ◽  
Özden Çobanoğlu ◽  
Eser Kemal Gürcan ◽  
Ertuğrul Kul ◽  
...  

In this study, the effect of STAT5A gene was investigated on milk yield and components (fat, protein) in Jersey cows. Because, this gene is a member of the signal transducers and activators of transcription the factor (STAT) family and thought to be the impact on milk yield and components. As a material in the research, 100 Jersey cows raised in Samsun Karakoy State Farm were used. Milk samples were collected 12 times with one month intervals after calving, and these samples were performed by ultrasonic milk analyzer for milk fat and protein content. In addition, test day milk yield (TDMY) of each cow were also recorded in the control days. C/G polymorphism with PCR-RFLP method result in DNA isolation from blood samples taken from cows was determined at 8.exon at position 12195. Genotype distribution was detected as 69% CC, 28% CG and 3% GG. Allele frequencies were found as 0.83 for the C allele and 0.17 for G allele. As a result of the polymorphism identified in STAT5A gene, statistically no difference were detected among genotypes for TDMY, 305 day milk yield (305 DMY), milk fat and protein rates and milk fat and protein yield. As a result, no significant differences were found between the milk yield and compositions association with STAT5A

2013 ◽  
Vol 58 (No. 7) ◽  
pp. 321-327 ◽  
Author(s):  
X.P. An ◽  
J.X. Hou ◽  
H.B. Zhao ◽  
L. Bai ◽  
J.Y. Peng ◽  
...  

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C&gt;T and g.6852C&gt;T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C<sup>&ndash;</sup> allele were 0.79&ndash;0.85, and frequencies of C<sup>+</sup> allele were 0.21&ndash;0.15. At STAT5A g.6852C&gt;T locus, frequencies of C allele were 0.70&ndash;0.72, and frequencies of T allele were 0.30&ndash;0.28. Compared with goats with DGAT1 C<sup>&ndash;</sup>C<sup>&ndash;</sup>, those with C<sup>&ndash;</sup>C<sup>+</sup> genotype had greater milk fat (P &lt; 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype (P &lt; 0.05). The results showed that does with C<sup>&ndash;</sup>C<sup>&ndash;</sup>CT and C<sup>&ndash;</sup>C<sup>+</sup>CT yielded more milk than those with C<sup>&ndash;</sup>C<sup>&ndash;</sup>CC(P &lt; 0.05). In addition, does with C<sup>&ndash;</sup>C<sup>+</sup>CT had the highest milk fat in comparison with other combination genotypes (P &lt; 0.05). &nbsp;


2021 ◽  
Vol 73 (3) ◽  
pp. 639-646
Author(s):  
C. Bondan ◽  
J.A. Folchini ◽  
L. Guimarães ◽  
M. Noro ◽  
R. Zanella ◽  
...  

ABSTRACT This study aimed to determine the impact of different post-partum disorders on milk yield and composition. One hundred and fifteen Holstein cows from a commercial dairy farm located in the state of Rio Grande do Sul in southern Brazil were monitored up to 62 days post-partum. During this period, body condition score evaluation and animal clinical examination were conducted. Percentages of fat, protein, and lactose, as well as somatic cells score, were determined in milk samples. The AST activity and concentrations of NEFA, calcium, and BHBA, were analyzed in blood samples. The occurrence of clinical disorders was identified in 30 (26%) cows. Subclinical disorders were identified in 64 (56%) cows. Only 21 (18%) cows did not suffer any kind of disorder within the studied period. In this study, no significant differences were found in milk production, protein, and somatic cell count in clinical, subclinical, and healthy cows. Milk fat and the fat: protein quotient (F:P) were higher in cows with clinical disorders and the 6 to 21 days in milk, and lactose were lower in cows with clinical disorders and the 22 to 42 days in milk (P<0.05).


Author(s):  
M. I. Kuziv ◽  
E. I. Fedorovych

The major components of economically useful traits in cattle dairy productivity, which conducted the selection is milk productivity and reproductive ability of cows. Studies conducted on animals Ukrainian black and white dairy cattle in the «Milk River» farm in the Sokal and Brody offices breeding reproducers «Breeder» Lviv region and plant breeding «Yamnytsya» Ivano–Frankivsk region. Ukrainian black and white dairy breed in the western region of Ukraine is characterized by good reproductive ability. Depending on the husbandry and lactation duration, service period lasted within 87.4 – 121.5 day, intercalving period – within 364 – 402.6 day, dry period within – 47.3 – 72.9 day, the fertility index was 46.3 – 48.0, coefficient of reproductive ability – within 0.92–0.99,  output of calves per 100 cows – within 91.8 – 98.8. The duration of pregnancy in cows of different  farms and lactations were in range 277 – 283.5 day and meet the requirements of the physiological norm. It has been shown that the milk yield of cows depends on the parameters of reproductive capacity. The correlation between duration of service–period and intercalving period, and milk yield, milk fat in most cases was positive. Correlation dry period duration and parameters of milk yield changed from negative to positive. Depending on the husbandry, lactation and index the impact share of service–period duration on milk yield was 7.0 – 29.7, for intercalving period – 8.8 – 31.8, for dry period – 9.2 – 23.8% respectively.


Author(s):  
N. Kosyachenko ◽  
Marina Abramova ◽  
M. Lapina

Abstract. Aim. The study of the influence of crossbriding on the growth and development of heifers and subsequent productive and reproductive qualities of cows on the first lactation. Methods. Using statistical methods, correlation analysis and ANOVA for evaluated genetic indicators of economic-valuable traits of dairy cattle. In particular was studied live weight of heifers from birth to 18 months, first insemination age and first fecundation age, live weight at fecundation, frequency of insemination, first calving age, days open, live weight at first lactation, milk yield for 305 days (kg), fat (%, kg) and protein (%, kg) in milk. Results. Relationship between productive indicators and the genotype of the animal is established. When assessing the strength of the influence of the factor «blood on the Holstein breed» it was found that the greatest impact can be traced at the age of 6 and 18 months, and is respectively 12.7 % and 17.1 %.The reproductive qualities of the first-calf cows decreased with the increase percent of blood in the Holstein breed. Evaluation of the impact of crossbreeding on milk production at first lactation has revealed significant strong influence on milk yield for 305 days of first lactation, and related signs the amount of milk fat and protein. According to qualitative indicators, the low influence of the Holstein blood fraction was found, while to a greater extent on the fat content than on the protein content in milk. Evaluation of phenotypic and genotypic correlations of milk yield and quality components of milk revealed average negative phenotypic and low negative genotypic correlation in all groups. The exception was in heifers of Yaroslavl breed with percent of Holstein blood less than 50 %, in which the relationship of yield x protein was positive. The relationship between milk yield and fat and protein content in milk has the opposite moderate character and strength. Practical significance. Studies was established that breeding of animals of Holstein and Yaroslavl breeds in one herd allows to receive an optimum ratio of milk production with optimal percentage fat and protein. For this herd among animals of the improved genotypes of the Yaroslavl breed, the use of genotypes with a percent of blood in the Holstein breed from 75.1 % to 87.6 % is optimal, which should be taken into account when selecting bulls to the breeding program.


2017 ◽  
Vol 53 ◽  
pp. 222-227
Author(s):  
T. M. Komendant

Objective: to study the dependence of productive longevity of cows of black and motley breed on genetically determined factors - linearity and bloodiness in Holstein breed. Methods: zootechnical, biological, statistical. The materials of the studies were pedigree cards of cows  and bulls, bull catalogs, zootechnical reports on pedigree work with the herd, the cattle base of the Grodno region, and also registers of rearing and rearing of the litter. To study the effect on the longevity of cows and their lifelong productivity of the "linear accessory" factor, all animals that were eliminated in 2006-2014 were divided into groups according to the lines: Adema 25437, Annas Adema 30587, Vis Eidial 933122, Montvik Chiftein 95679, Nico 31652 , Pabst Governera 882933, Reflection Sovering 198998, S. Sensation 1267271, Hiltes Adema 37910. Depending on the bloodiness of the Holstein breed, the animals were divided into four groups: I - up to 25% of Holstein genes, II- 25,0 – 49,9% of the genes of the Holstein genus, III - Holstein blood 50% and more, IV - purebred black-motley cows. Indicators of milk productivity (life-time milk yield (kg) and lifetime yield of milk fat (kg), milk yield for 1 day of lactation (kg), total duration of the lactation period (days) were studied in the experimental animals, and the duration of economic use (lactations). Main results. The results obtained during the statistical processing of data indicate that the cows belonging to the Pabst Governera line 882933 differed in the longest period of economic use among the abandoned animals. Duration of use in animals of this line was 8.43 lactations, which is more than for cows of other lines at 1,72-6,67 lactation. It should be noted that cows belonging to the Adem 25437 lines (6,71 lactations) and S. Sensation 1267271 (5,49 lactations) were also characterized by a rather long period of economic use. Due to the long period of use of the cow, the Pabst Governera line 882933 had the highest indicator of lifelong milk yield – 51535,58 kg of milk and the yield of milk fat – 1914,03. But as for milk for one day of lactation, here the leadership belonged to individuals belonging to the line Vis Eidial 933122 and amounted to 21,19 kg of milk per day. Also, a fairly high indicator of milk yield for one day of lactation was observed in animals belonging to the line Montvik Chiftain 95679 – 21,02 kg of milk. As a result of the research it was found that the largest term of economic use was characterized by low-breed hybrids with Holstein breed (up to 25%) – 3,63 lactations. This group of animals exceeded the individuals of the remaining groups by 0,21-0,76 lactations. It is worth noting that purebred black-motley individuals also differed by a long period of productive use – 3,42 lactations. Conclusions. Analysis of the impact on productive longevity and indicators of lifetime productivity of the "linearity" factor among abandoned animals in the "Progress-Vertelishki" made it possible to establish the existence of significant differences in the duration of economic use of cows of different linear affiliation. Cows of the Pabst Governera line 882933 – 8,43 lactations, 51535,58 kg of milk and 1914,03 kg of milk fat were distinguished by the greatest period of productive longevity, and also by the highest milk yield and milk fat content. It was established that the crossing of black and white cattle with Holstein breed positively influenced the productive longevity of animals, but on condition that the blood on the improving rock did not exceed 25%. Low-breed hybrids with Holstein breed exceeded the animals of other groups by the duration of their economic use by 0,21-0,76 lactations. Thus, we recommend breeding cows of black and motley breeds in the "Progress-Vertelishki", taking into account the impact on productive longevity and milk productivity of such factors as linearity and bloodiness in Holstein breed.


1978 ◽  
Vol 45 (3) ◽  
pp. 321-329 ◽  
Author(s):  
C. Lewis Johnson ◽  
David I. Kitchen

SummaryTwO Latin-square experiments using lactating Jersey cows have been made to determine the response to 4 levels of acetic acid given as a supplement to diets fed at Woodman's Standards. The response in milk yield and composition to daily supplementary infusions of acetic acid is curvilinear, the point of inflexion occurring between the levels of 450 and 600 ml acetic acid/d. The direction of responses to the 150-ml level of infusion varied between the experiments. At the 600-ml level of addition the non-significant increases in milk fat, protein and lactose yield led to a significant increase in solids corrected milk yield. The effects of acetic acid infusion on the composition of the milk fat were determined.


1974 ◽  
Vol 25 (4) ◽  
pp. 657 ◽  
Author(s):  
TH Stobbs ◽  
DJ Brett

Jersey cows were used in a change-over design to examine the effect of three levels of energy intake (lucerne hay at 100, 75 and 50% ad lib.) on milk yield, milk composition, fatty acid composition of milk fat, and blood metabolites (non-esterified fatty acids, glucose and total ketones) to determine which measurement was the most accurate indicator of intake of energy. Milk yields averaged 9.9, 8.7 and 7.2 kg/cow/day with relative intakes of 100, 75 and 50% of ad lib. When energy was restricted the proportion of C4–C16 fatty acids in milk fat decreased (72, 69 and 59%), while the proportion of oleic acid increased (15, 18 and 26%). These changes occurred within approximately 6 days on new energy levels. Fore milk and strippings had similar fatty acid proportions. Restriction of energy reduced the solids not fat, protein and casein contents of milk, and increased its butter fat percentage. Non-esterifred fatty acid levels in blood plasma increased with restriction of feed (348, 528 and 579 µ-equiv./l). Glucose and ketone bodies of blood averaged 58 mg/100 ml and 9.1 mg/100 mi respectively, and did not vary between treatments. It is concluded that milk production is the most sensitive indicator of the intake of digestible energy where change-over designs are used. However, when individual animal variation is not removed in the analysis, the intake of energy is most closely correlated with the fatty acid composition of milk fat (r = 0.73 and –0.74 for C4–C16 acids and oleic acid respectively). Significant correlations with the protein to fat and casein to fat ratios of milk were also measured (r = 0.64 and 0.63 respectively). There was a poor relationship between energy intake and blood composition (r = –0.25 for non-esterified fatty acid content).


2006 ◽  
Vol 49 (3) ◽  
pp. 236-249 ◽  
Author(s):  
K. Kovács ◽  
J. Völgyi-Csík ◽  
A. Zsolnai ◽  
I. Györkös ◽  
L. Fésüs

Abstract. The aim of this paper was to study the polymorphisms of bovine growth hormone gene. The authors genotyped 363 Hungarian Holstein-Friesian bull dams from 6 farms all over the country. Two variants (L and V) of the bovine growth hormone gene digested with AluI enzyme were identified in the experiment. Genotyping was carried out by PCR-RFLP method. The frequency data of L and V allele was 0.93 and 0.07 respectively. Distribution of the three genotypes were 87.05% (LL), 12.40% (LV) and 0.55% (VV). The studied population was in H-W equilibrium considering the genotype distribution. SPSS 11.0 for Windows was used to reveal the possible correlations between GH genotypes and production and reproduction traits and further statistical analyses. On the basis of statistical analyses it can be found that VV genotype cows had the longest milking period and LL had the shortest dry period. Both differences were significant. Cows with LV genotype had significantly higher test milking data than LL cows. Furthermore, LV genotype seemed to be advantageous for 305 days lactation milk yield. While milk composition traits, as 305 days milk fat and protein percent showed the opposite tendency, since LL genotyped dams produced significantly higher values in these traits.


2017 ◽  
Vol 19 (74) ◽  
pp. 52-56
Author(s):  
V. V. Fedorovych

It is well known with an increase of milk productivity of cows their reproductive capacity in general became worse. Therefore, it is important to establish optimal parameters of the first age insemination and first calving, duration of service- and inter-calving periods. In view of the above, the purpose of our researches was to study the influence of reproductive capacity parameters of Simmental cows on the formation of milk productivity. The study were conducted on 331 cows in agricultural LLC «Litynskyy» Drohobych raion, Lviv oblast. Evaluation of milk productivity and reproductive capacity of the animals (in 20 years) were performed according to the data of zootechnical accounting for the first, second, third and the best lactations. The differences in rates of milk productivity depending on the parameters of their reproductive capacity were established. Animals with age of first fruitful insemination 18.1–20.0, with first calving age – 27.1–29.0, duration of service period – 101–120 and duration of the inter-calving period – 381–400 days had the best yields and the highest quantity of milk fat. According to the above mentioned indicators of milk productivity they are likely dominated by animals with bigger or smaller indicators of reproductive capacity, but in most cases, this advantage was unreliable. It was found positive highly significant links between indicators of reproductive capacity of cows and milk yield. The highest correlation coefficients were observed between age of first calving and their milk yields, depending on lactation, they were 0.380–0.498, lower – between age of first insemination and milk yield – 0.316–0.456 and the lowest – between service- and inter-calving periods and yield – 0.124–0.335; 0.127–0.331 respectively. The impact of the abovementioned indicators on yield was within 23.13–39.88; 28.63–45.02; 30.98–35.17%, respectively. 


Author(s):  
P.H. Paradkar ◽  
V.M. Loke ◽  
C.G. Godse ◽  
R.A. Vaidya ◽  
A.D.B. Vaidya

Background: Collective evidence of polymorphic β-casein and associated health problems has led to the concern about milk consumption and cow breeding policies worldwide. This association has also engrossed the interest of dairy scientist and industry in evaluation of β casein genotype distribution. With increasing proportion of exotic and crossbred cows in India it is worth while to screen cattle for A1A2 β casein and enhance indigenous cow breeds. Methods: The present study intended to identify β casein genotypes in pure Indian Gir cows and crossbred Holstein and jersey cows from three local dairy farms. We analysed β casein genotypes by PCR-RFLP method in total 95 cows during the period of 2017-2019. Result: All the indigenous Gir cows had fixed A2 allele whereas crossbred Jersey and Holstein Frisian both had A1A2 as the most common genotype (frequency: 0.473 and 0.6 respectively) followed by A2A2 (Frequency 0.368 and 0.333 respectively) and A1A1 (Frequency 0.158 and 0.066 respectively). The results show that in this study group Gir, a native Indian breed has fixed A2 β casein variant whereas crossbred Jersey and Holstein Frisian have A1A2 as a most common genotype. Screening of cattle for â casein genotypes is vital to monitor the frequency of A1 beta casein in native Indian cow breeds.


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