scholarly journals Identification of β Casein Genotypes in Indian Gir and Crossbred Exotic Cows from Mumbai Dairy Farms

2021 ◽  
Author(s):  
P.H. Paradkar ◽  
V.M. Loke ◽  
C.G. Godse ◽  
R.A. Vaidya ◽  
A.D.B. Vaidya
Keyword(s):  
Dairy Farms ◽  
Genotype Frequency ◽  
Study Group ◽  
Milk Consumption ◽  
Genotype Distribution ◽  
Crossbred Cows ◽  
Pcr Rflp ◽  
Beta Casein ◽  
Genotype Screening ◽  
Jersey Cows

Background: Collective evidence of polymorphic β-casein and associated health problems has led to the concern about milk consumption and cow breeding policies worldwide. This association has also engrossed the interest of dairy scientist and industry in evaluation of β casein genotype distribution. With increasing proportion of exotic and crossbred cows in India it is worth while to screen cattle for A1A2 β casein and enhance indigenous cow breeds. Methods: The present study intended to identify β casein genotypes in pure Indian Gir cows and crossbred Holstein and jersey cows from three local dairy farms. We analysed β casein genotypes by PCR-RFLP method in total 95 cows during the period of 2017-2019. Result: All the indigenous Gir cows had fixed A2 allele whereas crossbred Jersey and Holstein Frisian both had A1A2 as the most common genotype (frequency: 0.473 and 0.6 respectively) followed by A2A2 (Frequency 0.368 and 0.333 respectively) and A1A1 (Frequency 0.158 and 0.066 respectively). The results show that in this study group Gir, a native Indian breed has fixed A2 β casein variant whereas crossbred Jersey and Holstein Frisian have A1A2 as a most common genotype. Screening of cattle for â casein genotypes is vital to monitor the frequency of A1 beta casein in native Indian cow breeds.

scholarly journals Jersey İneklerinde STAT5A Geninin Süt Verimi ve Bileşenleri Üzerine Etkisinin Araştırılması

2014 ◽  
Vol 2 (6) ◽  
pp. 289
Author(s):  
Soner Çankaya ◽  
Mehmet Ülker ◽  
Özden Çobanoğlu ◽  
Eser Kemal Gürcan ◽  
Ertuğrul Kul ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Dna Isolation ◽  
Genotype Distribution ◽  
State Farm ◽  
Signal Transducers ◽  
Pcr Rflp ◽  
The Impact ◽  
Jersey Cows ◽  
Stat5a Gene

In this study, the effect of STAT5A gene was investigated on milk yield and components (fat, protein) in Jersey cows. Because, this gene is a member of the signal transducers and activators of transcription the factor (STAT) family and thought to be the impact on milk yield and components. As a material in the research, 100 Jersey cows raised in Samsun Karakoy State Farm were used. Milk samples were collected 12 times with one month intervals after calving, and these samples were performed by ultrasonic milk analyzer for milk fat and protein content. In addition, test day milk yield (TDMY) of each cow were also recorded in the control days. C/G polymorphism with PCR-RFLP method result in DNA isolation from blood samples taken from cows was determined at 8.exon at position 12195. Genotype distribution was detected as 69% CC, 28% CG and 3% GG. Allele frequencies were found as 0.83 for the C allele and 0.17 for G allele. As a result of the polymorphism identified in STAT5A gene, statistically no difference were detected among genotypes for TDMY, 305 day milk yield (305 DMY), milk fat and protein rates and milk fat and protein yield. As a result, no significant differences were found between the milk yield and compositions association with STAT5A

PCR-RFLP Detection od PAI-2 Gene Variants: Prevelence in Ethnic Groups and Disease Relationship in patients Undergoing corony Angiography

1997 ◽  
Vol 77 (05) ◽  
pp. 0955-0958 ◽  
Author(s):  
Carole A Foy ◽  
Peter J Grant
Keyword(s):  
Gene Variants ◽  
Genotype Distribution ◽  
Pima Indians ◽  
Significant Difference ◽  
Pcr Rflp ◽  
History Of ◽  
Caucasian Patients ◽  
Clinical Conditions ◽  
Rflp Assay ◽  
Coronary Atheroma

SummaryPAI-2 is a fibrinolytic inhibitor produced predominantly by monocytes. Most PAI-2 is intracellular making study in clinical conditions difficult. Abnormalities in production may be associated with inflammation and fibrinolysis at sites of tissue damage such as the atherosclerotic plaque.PAI-2 gene variants have been described: variant A consists of Asn120, Asn404 and Ser413 and variant B consists of Asp120, Lys404 and Cys413. We designed a PCR-RFLP assay using primers spanning the region containing Asn/Lys404 and Ser/Cys413. Variant B contains an Mwol restriction site. We analysed 302 Pima Indians and 286 healthy Caucasian volunteers. To investigate relationships between genotype and vascular disease we analysed 333 Caucasian patients undergoing coronary angiography.Gene variant B was more common in the Pimas than in Caucasians (p <0.0001). There was no significant difference in genotype distribution between the volunteers and patients. In the patients there was no association between genotype and either a history of MI or extent of coronary atheroma.

CYP3A4 *1B Gene Polymorphism in Coronary Artery Disease Patients with Obesity Undergoing Statin Treatment

2021 ◽  
Vol 18 ◽  
Author(s):  
Elifcan Gezer ◽  
Mehtap Cevik ◽  
Cansu Selcan Akdeniz ◽  
Ismail Polat Canbolat ◽  
Selen Yurdakul ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Statin Therapy ◽  
Plasma Lipid ◽  
Blood Cholesterol ◽  
Study Group ◽  
Genotype Distribution ◽  
Obese Patients ◽  
Artery Disease

Objective: Coronary artery disease (CAD) is the one of the leading cause of morbidity and mortality worldwide and statins are frequently prescribed in the treatment of CAD to help lower blood cholesterol levels. Since the main enzyme involved in the metabolism of statins is CYP3A4, we aimed to investigate the effect of CYP3A4 * 1B genotypes on plasma lipid profile in Turkish cardiovascular disease subject with and without obesity taking statin. Materials and Methods: The study group consisted of 85 cardiovascular disease patients who were prescribed statins and had routine biochemical analysis data. Polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) assay were performed for genotyping of CYP3A4 *1B (rs2740574) polymorphism. Results: Genotype distribution of CYP3A4 *1B polymorphism was found for homozygous wild (AA) and homozygous polymorphic (GG) genotypes as 94.1% and 5.9% respectively. We did not detect patients with heterozygous genotype in our study group. We found that the mean LDL-c, TG and TC levels were higher in patients with CYP3A4 *1B GG compared to AA genotype. The frequency of CYP3A4 *1B GG genotype frequency (9.5%) was detected higher in the obese patients compared to the non-obese patients (7.7%) (χ2=0.037, p=0.85). Conclusions: Our results demonstrate that CYP3A4 *1B homozygous polymorphic genotype distribution tend to be higher in obese patients compared to non- obese patients with cardiovascular disease which may point *1B allele to have a slight effect on serum lipids during statin therapy. Additional studies with higher samples are needed for evaluating the role of CYP3A4 *1B on lipids in patients under statin therapy.

scholarly journals MDR1 polymorphisms are not related to Xeliri and Xelox chemoresistance in colorectal cancer

2019 ◽  
Author(s):  
Ayat B. Al-Ghafari ◽  
Areej M. Alqahtani ◽  
Suzan N. Alturki ◽  
Huda Abdulaziz Al Doghaither ◽  
Hanaa M. Tashkandi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Drug Response ◽  
Fragment Length Polymorphism ◽  
Protective Role ◽  
Genotype Distribution ◽  
Chain Reaction ◽  
P Glycoprotein ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Polymerase Chain

Abstract Background Multidrug resistance member 1 (MDR1) is located on chromosome 7 and encodes P-glycoprotein (Pgp), which is universally accepted as a drug resistance biomarker. MDR1 polymorphisms may change either the protein expression or function, suggesting its possible association with cancers, including colorectal cancer (CRC). Thus, this study aimed to determine the effects of MDR1 polymorphisms on the drug response of Saudi CRC patients.Methods DNA samples were obtained from 62 CRC patients and 100 healthy controls. The genotypes and allele frequencies of the MDR1 polymorphisms G2677T and T1236C were determined by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP).Results No significant difference was observed in the genotype distribution and allele frequency of T1236C between the CRC the patients and the controls. However, G2677T was found to play a highly significant protective role against the progression of CRC. Moreover, the results showed that none of the genotypes in SNPs T1236C and G2677T affected chemoresistance to Xeliri and Xelox.Conclusions T1236C in the MDR1 gene is not related to CRC risk, and G2677T protects against the development of CRC. Both MDR1 polymorphisms are not associated with the risk of chemoresistance.

CAT-21 A/T Gene Polymorphisms Associated with Breast Cancer Susceptibility

2021 ◽  
Vol 32 (2) ◽  
pp. 79-84
Author(s):  
Kevin Owen ◽  
Siti Syarifah ◽  
Mutiara Indah Sari
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cancer Susceptibility ◽  
Breast Cancer Susceptibility ◽  
Healthy Control Group ◽  
Control Group ◽  
Genotype Distribution ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Healthy Control

Background: Oxidative stress induced cancer cell formation. Gene polymorphism plays roles in carcinogen metabolism, antioxidant and DNA repairing pathway was susceptibility to oxidative stress. This study aim to determine the association between CAT-21 A/T polymorphism with breast cancer susceptibility. Methods: Case control study was conducted on 65 breast cancer patient and 65 healthy control group. The whole blood samples were isolated from 65 breast cancer patients in Haji Adam Malik General Hospital Medan and 65 healthy control group. The CAT-21A/T polymorphism was analyzed by PCR-RFLP procedure. PCR-RFLP product was electrophoresed and visualized in agarose 4%. Results:The AA CAT-21 genotype were lower in breast cancer (BC) than healthy control (HC) group (31/47.7% vs 40/61.5%), in the contrary AT+TT genotype was greater in BC than HC group (34/52.3% vs 25/38.5%) with (p=0.159, OR=1.755, CI=0.874–3.525). A allele CAT-21 were found lower in BC than HC group (89/68.5% vs 105/80.8%) then T allele were greater in BC than HC group (41/31.5% vs 25/19.2%) with (p=0.033, OR=1.935;CI=1.022-3.428). Conclusions: There was significant difference in allele distribution of CAT-21 A/T between case and control group but no in genotype distribution. In this population study showed that allele of CAT -21 A/T polymorphism could represent as a risk factor to breast cancer. Bangladesh J Medicine July 2021; 32(2) : 79-84

Postpartum anoestrous interval in first-lactation beef and dairy-beef crossbred cows

2012 ◽  
Vol 52 (7) ◽  
pp. 478 ◽  
Author(s):  
R. E. Hickson ◽  
R. L. Laven ◽  
N. Lopez-Villalobos ◽  
P. R. Kenyon ◽  
S. T. Morris
Keyword(s):  
New Zealand ◽  
Pregnancy Rate ◽  
Milk Yield ◽  
Beef Breed ◽  
Crossbred Cows ◽  
Subsequent Fertility ◽  
Jersey Cows ◽  
Fertility Traits ◽  
Dairy Breed ◽  
Friesian Cows

In New Zealand, beef-breeding cows are required to wean a calf every year, and the weight of calf at weaning is a major driver of the efficiency of the breeding cow. Weaning weight can be increased by increasing milk yield of the cow such as by incorporating dairy breed genetics into the breeding cows, and by having early born calves which are, therefore, older at weaning. This experiment examined postpartum anoestrous interval and pregnancy rate to rebreeding in 109 beef-breed and beef-cross-dairy breed first-lactation cows suckling their own calves. Cows with a Jersey component had a shorter postpartum anoestrous interval than cows without (90 versus 97–101 days; P < 0.05). Pregnancy rates after 42 days of joining with bulls were 83–97% for straight-bred Angus, Angus-cross-Friesian, Angus-cross-Jersey and Angus-cross-Kiwicross (Friesian-cross-Jersey) cows, but only 41% for straight-bred Friesian cows (P < 0.05). For every day younger heifers reached puberty, postpartum anoestrous interval was reduced by 0.07 ± 0.02 days (P < 0.01) indicating that age at puberty could be used as a predictor of subsequent fertility. The Jersey-component cows offered advantages in fertility traits and may be worthwhile incorporating into beef-breeding cow herds.

scholarly journals Milk Safety Assessment, Isolation, and Antimicrobial Susceptibility Profile of Staphylococcus aureus in Selected Dairy Farms of Mukaturi and Sululta Town, Oromia Region, Ethiopia

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Sema Regasa ◽  
Shimelis Mengistu ◽  
Ashebr Abraha
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Dairy Farms ◽  
Raw Milk ◽  
Penicillin G ◽  
Human Consumption ◽  
Milk Consumption ◽  
Cross Sectional ◽  
Antimicrobial Susceptibility Profile

A cross-sectional study was conducted to estimate the prevalence of Staphylococcus aureus in raw milk and swab, to assess the antimicrobial susceptibility profile, milk handling practice, and its associated risk factors in selected dairy farms of Mukaturi and Sululta Town, Oromia Region, Ethiopia. A total of 247 samples collected from dairy farms were examined using standard microbiological techniques. The antimicrobial susceptibility profiles of the isolates were also investigated. The possible risk factors for Staphylococcus aureus contaminations in milk were evaluated through a structured questionnaire. Overall, 16.6% (n= 41) of the samples were positive for S. aureus. The prevalence of S. aureus was 15.3% from udder milk and 25%, 20%, and 10% from milkers’ hand, milking bucket, and drying towel swab, respectively. The prevalence of Staphylococcus aureus in milk showed statistically significant variation with respect to age (p≤ 0.001), parity (P≤ 0.001), drainage condition of milking area (P=0.035), study sites (P=0.035), and management system (P=0.035). Majority of the isolates were found resistant to penicillin G (97.6%) and amoxicillin (43.9%). According to this study, 12/28(42.9%) Staphylococcus aureus positive raw milk samples had 104-105cfu/ml S. aureus count, which is above the recommended level for human consumption. 47.1% of milking persons store milk at room temperature temporarily (between 6 and 12 hrs) till transport to collection center with no means of cooling aid. From consumers 25.6% had no health risk associated with raw milk consumption or aware of milk borne disease associated with drinking raw milk. Thus, 60.5% of milk users had habit of raw milk consumption. The study revealed a prevalence of S. aureus, poor milk handling practices, raw milk consumption behavior in study area. Proper handling and hygiene decrease milk contamination by S. aureus and make it safe for human consumption.

scholarly journals Evaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy

2017 ◽  
Vol 6 (3) ◽  
Author(s):  
Elisa Massella ◽  
Silvia Piva ◽  
Federica Giacometti ◽  
Gaetano Liuzzo ◽  
Angelo Vittorio Zambrini ◽  
...  
Keyword(s):  
Risk Factor ◽  
Dairy Cattle ◽  
Rapid Method ◽  
Low Cost ◽  
Dairy Farms ◽  
Northern Italy ◽  
Common Variants ◽  
Milk Intolerance ◽  
Beta Casein ◽  
Sequences Analysis

Bovine beta casein A1 is one of the most common variants in dairy cattle breeds; it is considered a risk factor in milk intolerance and in other important human diseases, because of the bioactive peptide beta casomorphin-7 (BCM7) produced by raw or processed A1-milk, but not by A2- milk, during digestion. The aim of this study was to perform a cheap and rapid method to investigate beta casein polymorphism in copious animals. The study included 2 dairy farms with a totally of 1230 cows. Beta casein genotypes were estimated evaluating Exon 7 region of bovine beta casein gene (CSN2) by sequences analysis. In the population included in the study 5 variants (A1, A2, B, F, I) and 13 genotypes (A1A1, A1A2, A1B, A1F, A1I, A2A2, A2B, A2F, A2I, BB, BF, BI, FI) were detected. The method showed high sensibility and specificity, resulted low-cost and few time consuming.

scholarly journals Analysis of Slovak Spotted breed for bovine beta casein A1 variant as risk factor for human health.

1970 ◽  
Vol 60 (4) ◽  
Author(s):  
Martina Miluchová ◽  
Michal Gábor ◽  
Anna Trakovická
Keyword(s):  
Diabetes Mellitus ◽  
Genomic Dna ◽  
Total Population ◽  
Human Consumption ◽  
Effective Number ◽  
Immune Systems ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Beta Casein ◽  
Commercial Kit

The goal of work was identification A1 variant of bovine beta casein which involves ischemic heart disease and diabetes mellitus in human. The digestion of A1beta casein can result in the production of bioactive beta casomorphin-7 (BCM-7); this is not the case with A2. This bioactive peptide has been linked to physiological traits that may elicit effects on components of the vascular and immune systems. The material involved 111 Slovak Spotted breed. Bovine genomic DNA was extracted from whole blood by using commercial kit, and used in order to estimate beta-casein genotypes by means of PCR-RFLP method. The PCR products were digested with DdeI restriction enzyme. In the population included in the study were detected all three genotypes, homozygote genotype A1A1 (14 animals), heterozygote genotype A1A2 (37 animals) and homozygote genotype A2A2 (60 animals). In the total population of cattle homozygotes A2A2-0.5405 were the most frequent, while homozygotes A1A1-0.1261 were the least frequent ones. This suggests a superiority of allele A2 (0.7072) which does not produce BCM-7, and thus is safe for human consumption. The expected homozygosity for gene CSN2 is in the population stated a slight increase in homozygosity (0.5858). This caused a slight decrease in the level of possible variability realization (41.80%), which corresponds to the effective number of alleles (1.7071).

scholarly journals ABCB1 variants C3435T and T129C are not associated with colorectal cancer risk

2019 ◽  
Vol 19 (3) ◽  
pp. 2476-2483 ◽  
Author(s):  
Areej M Al Qahtani ◽  
Ayat B Al-Ghafari ◽  
Huda A Al Doghaither ◽  
Anas H Alzahrani ◽  
Ulfat M Omar ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Saudi Arabia ◽  
Goodness Of Fit ◽  
Allele Frequencies ◽  
Genotype Distribution ◽  
Exact Test ◽  
Weinberg Equilibrium ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
And Control

Background: Colorectal cancer (CRC) is one of the most prevalent cancers in Saudi Arabia that is highly characterized with poor survival rate and advanced metastasis. Many studies contribute this poor outcome to the expression of ABC transporters on the surface of cancer cells.Objectives: In this study, two ABCB1 variants, C3435T and T129C, were examined to evaluate their contribution to CRC risk.Methods: 125 subjects (62 CRC patients and 63 healthy controls) were involved. The DNA was isolated and analyzed with PCR-RFLP to determine the different genotypes. The hardy-Weinberg equilibrium was performed to determine genotype distribution and allele frequencies. Fisher’s exact test (two-tailed) was used to compare allele frequencies between patients and control subjects. Results: The study showed that for SNP C3435T, the population of both CRC patients and controls were out of Hardy-Weinberg equilibrium. Genotype distribution for CRC patients was (Goodness of fit χ2 = 20, df= 1, P≤0.05), whereas, for the controls the genotype distribution was (Goodness of fit χ2 = 21, df =1, P ≤0.05). For SNP T129C, all subjects showed normal (TT) genotype.Conclusion: There was no significant association between ABCB1 3435C>T and 129T>C polymorphisms with CRC risk.Keywords: Colorectal cancer, ABCB1 gene, SNP C3435T, SNP T129C, PCR-RFLP, Saudi Arabia.

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