scholarly journals Functional Quality and Colour Attributes of Two High-Lycopene Tomato Breeding Lines Grown under Greenhouse Conditions

2016 ◽  
Vol 4 (5) ◽  
pp. 365 ◽  
Author(s):  
Ilahy Riadh ◽  
Siddiqui Mohammed Wasim ◽  
Tlili Imen ◽  
Piro Gabriela ◽  
Lenucci Marcello Salvatore ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Rio Grande ◽  
Total Vitamin ◽  
Total Carotenoids ◽  
Breeding Lines ◽  
Functional Quality ◽  
Fresh Cut ◽  
Β Carotene

This study investigates the antioxidant components (total carotenoids, lycopene, β-carotene, total phenols, flavonoids, ascorbic acid and total vitamin C) as well as the hydrophilic and lipophilic antioxidant activities (HAA and LAA) of tomato genotypes consisting of one ordinary cultivar Rio Grande and the two high-lycopene breeding lines HLT-F71 and HLT-F72. The correlation of nutritional value to L∗, a∗, b∗ colour indexes and a∗/b∗ ratio was also investigated in whole and fresh cut tomato fruits. Except for β-carotene content, significant differences were found among cultivars for antioxidants. The berries of both HLT-lines recorded higher antioxidant contents, HAA and LAA (TEAC and FRAP assays) than Rio Grande. Under controlled conditions, HLT-F72 reached the highest levels of total carotenoids (165.5 mg β-ca Eq per kg fw), lycopene (150.1 mg per kg fw) and total phenolics (549.7 mg GAE per kg fw). However, HLT-F72 exhibited the highest levels of ascorbic acid (193.3 mg per kg fw), total vitamin C (271.6 mg per kg fw) and flavonoids (450.5 mg RE per kg fw). Line HLT-F71 showed the highest HAA (148.0 µM Trolox per 100 g fw and 4.2 mM FRAP per g fw) and LAA values (258.5 µM Trolox per 100 g fw and 3.2 mM FRAP per g fw respectively). Colour readings in red ripe fresh cut tomato berries particularly a∗, b∗ and the ratio a∗/b∗ could represent an indicator not only for lycopene but also for other phytochemicals and resulting antioxidant activities. HLT-Lines may serve as a starting point for the development of semi-determinate growth habit tomato cultivars with higher functional quality.

scholarly journals Bioactive compounds and antioxidant activity in tomatoes (Lycopersicon esculentum L.) cultivars in natura and after thermal processing

2020 ◽  
Vol 9 (11) ◽  
pp. e44991110192
Author(s):  
Angela Vacaro de Souza ◽  
Jéssica Marques de Mello ◽  
Vitória Ferreira da Silva Fávaro ◽  
Victória Farias da Silva ◽  
Diogo de Lucca Sartori ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Lycopersicon Esculentum ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Thermal Processing ◽  
Antioxidant Activities ◽  
The Impact ◽  
Β Carotene

The aim of the study was to evaluate the impact of processing by cooking in natura fruits on the content of bioactive compounds - vitamin C and ascorbic acid, phenolic compounds, flavonoids, carotenoids and anthocyanins, lycopene and β-carotene - and on antioxidant activity - DPPH and FRAP - of 9 varieties of commercial and non-commercial tomatoes. The fruits were harvested when they reached the point of physiological maturation, selected and evaluated in natura, and after grinding and cooking for 30 minutes. At the end of the experiment it was found that all bioactive compounds analyzed showed quantitative reductions when the fruits were processed, with cherry cultivars - 7, 8 and 9 - those that showed superior results compared to different bioactive compounds evaluated, proving to be interesting to be better explored. Regarding the antioxidant activities, the processed fruits of these cultivars showed less losses showing potential to be submitted to processing.

scholarly journals Associations between antioxidants and all-cause mortality among US adults with obstructive lung function

2014 ◽  
Vol 112 (10) ◽  
pp. 1662-1673 ◽  
Author(s):  
Earl S. Ford ◽  
Chaoyang Li ◽  
Timothy J. Cunningham ◽  
Janet B. Croft
Keyword(s):  
Lung Function ◽  
Vitamin C ◽  
Smoking Status ◽  
Chronic Obstructive ◽  
Continuous Variables ◽  
Total Carotenoids ◽  
Obstructive Pulmonary Disease ◽  
Health And Nutrition ◽  
All Cause Mortality ◽  
Β Carotene

Chronic obstructive pulmonary disease is characterised by oxidative stress, but little is known about the associations between antioxidant status and all-cause mortality in adults with this disease. The objective of the present study was to examine the prospective associations between concentrations of α- and β-carotene, β-cryptoxanthin, lutein/zeaxanthin, lycopene, Se, vitamin C and α-tocopherol and all-cause mortality among US adults with obstructive lung function. Data collected from 1492 adults aged 20–79 years with obstructive lung function in the National Health and Nutrition Examination Survey III (1988–94) were used. Through 2006, 629 deaths were identified during a median follow-up period of 14 years. After adjustment for demographic variables, the concentrations of the following antioxidants modelled as continuous variables were found to be inversely associated with all-cause mortality among adults with obstructive lung function: α-carotene (P= 0·037); β-carotene (P= 0·022); cryptoxanthin (P= 0·022); lutein/zeaxanthin (P= 0·004); total carotenoids (P= 0·001); vitamin C (P< 0·001). In maximally adjusted models, only the concentrations of lycopene (P= 0·013) and vitamin C (P= 0·046) were found to be significantly and inversely associated with all-cause mortality. No effect modification by sex was detected, but the association between lutein/zeaxanthin concentrations and all-cause mortality varied by smoking status (Pinteraction= 0·048). The concentrations of lycopene and vitamin C were inversely associated with all-cause mortality in this cohort of adults with obstructive lung function.

Streamlined three step total vitamin C analysis by HILIC-UV for laboratory testing

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Michael Fitzpatrick ◽  
Paul Bonnitcha ◽  
Van Long Nguyen
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Laboratory Testing ◽  
Potassium Phosphate ◽  
Internal Standard ◽  
Good Precision ◽  
Hydrophilic Interaction ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Total Vitamin

Abstract Objectives In the clinical setting, the analysis and quantification of vitamin C (ascorbic acid) poses several challenges including analyte instability and poor retention by reverse phase HPLC systems. In this article we describe a rapid hydrophilic interaction chromatography ultraviolet method for the measurement of total vitamin C in plasma which overcomes these issues. Methods Ascorbic acid and the internal standard were separated under isocratic conditions using a Waters BEH-Amide column and a mobile phase containing 0.005 M potassium phosphate in 80% acetonitrile. Results The proposed method was validated and showed good precision (coefficient of variation <5%), accuracy (>99%), and analyte stability after extraction (>24 h). Conclusions The simple sample preparation allows full automation and rapid analytical run times of the assay and is therefore suitable for a high-throughput clinical chromatography laboratory.

Determination of Total Vitamin C by Ion Exclusion Chromatography with Electrochemical Detection

1989 ◽  
Vol 72 (4) ◽  
pp. 681-686
Author(s):  
Hie-Joon Kim
Keyword(s):  
Ascorbic Acid ◽  
Sulfuric Acid ◽  
Vitamin C ◽  
Retention Time ◽  
Acid Content ◽  
High Speed ◽  
Reference Electrode ◽  
Dehydroascorbic Acid ◽  
Total Vitamin

Abstract A rapid and sensitive liquid chromatographic method for determination of total vitamin C in foods and beverages is described. Ascorbic acid and dehydroascorbic acid are extracted with sulfuric acid solution, and the dehydroascorbic acid in the extract is reduced to ascorbic acid by dithiothreitol at pH 7. The reduction is complete in 2 min at room temperature. The resulting total ascorbic acid is separated on an anion exclusion/high speed column with 20mM sulfuric acid as eluant and detected amperometrically with a platinum electrode operating at +0.6-0.8 V vs Ag/AgCl reference electrode. Dithiothreitol (retention time, 3.2 min) does not interfere with the separation and detection of ascorbic acid (retention time, 1.3 min). The dehydroascorbic acid content can be estimated as the difference in ascorbic acid content measured with and without reduction by dithiothreitol. The completeness of the reduction was demonstrated by purposely allowing the oxidation of ascorbic acid in the food extract and determining the total vitamin C after reduction. The determinations of vitamin C content in selected foods and beverages were in good agreement with the expected values. Total analysis time for vitamin C is 10 min and the detection limit is 0.1 ng. The method is specific for vitamin C, and interference by other food constituents is minimal.

Automated Fluorometric Method for the Determination of Total Vitamin C in Food Products

1976 ◽  
Vol 59 (6) ◽  
pp. 1244-1250 ◽  
Author(s):  
Ram B Roy ◽  
Aldo Conetta ◽  
Jerry Salpeter
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Dehydroascorbic Acid ◽  
Food Products ◽  
Total Vitamin ◽  
Automated Method ◽  
Highly Sensitive ◽  
Aoac Method ◽  
Analytical System

Abstract A specific microfluorometric method for the determination of ascorbic acid, dehydroascorbic acid, and total vitamin C in food products has been automated. The procedure developed is an adaptation of the official AOAC method (secs. 43.056–43.062), except that N-bromosuccinimide is used instead of Norit to oxidize vitamin C. Ascorbic acid is selectively oxidized by N-bromosuccinimide before other interfering substances that may be present, so this method is a highly sensitive and specific technique with extensive applicability. The proposed automated method is simple, rapid, reliable, and sufficiently sensitive to analyze as little as 2 × 10−3 to 0.1 mg ascorbic acid/ml. Analytical results obtained for ascorbic acid, dehydroascorbic acid, and total vitamin C in a wide variety of food products are reported. The analytical system developed has the capability of analyzing 50 samples/hr.

scholarly journals Effects of selected agronomical factors on the content of health-promoting compounds in spaghetti squash (Cucurbita pepo L. subsp. pepo)

2012 ◽  
Vol 24 (1) ◽  
pp. 33-39
Author(s):  
Wanda Wadas ◽  
Halina Mioduszewska ◽  
Krzysztof Kalinowski
Keyword(s):  
Ascorbic Acid ◽  
Cucurbita Pepo ◽  
Sowing Date ◽  
Carotenoid Content ◽  
Plant Spacing ◽  
Total Carotenoid ◽  
Total Carotenoids ◽  
Total Carotenoid Content ◽  
Health Promoting ◽  
Β Carotene

Effects of selected agronomical factors on the content of health-promoting compounds in spaghetti squash (Cucurbita pepoL. subsp.pepo)The study examined the effects of the sowing date (5, 15 and 25 May) and plant spacing (1 × 0.6 m, 1 × 0.8 m and 1 × 1 m) on the content of carotenoids and L-ascorbic acid in the fruits of two cultivars of spaghetti squash (‘Makaronowa Warszawska' and ‘Pyza’). The field experiment was carried out in central-eastern Poland. Fully mature spaghetti squash fruits were harvested from the beginning of September to the beginning of October. The content of total carotenoids and β-carotene was the greatest in the fruits of plants sown on 15 May. The sowing date did not affect the content of L-ascorbic acid. Increasing the plant spacing from 1 × 0.6 m to 1 × 1 m resulted in an increase of total carotenoid content, particularly for the spaghetti squash sown on 25 May. Plant spacing did not have a significant effect on the content of β-carotene and L-ascorbic acid. Fruits of the ‘Makaronowa Warszawska’ with light orange flesh contained more total carotenoids and β-carotene than fruits of the ‘Pyza’ with cream-coloured flesh. The content of L-ascorbic acid in the fruits of both cultivars did not differ significantly.

scholarly journals Influence of Emollient on the Preparation and stability of Sodiun Ascorbyl Phospate Cream

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Yanni Dhiani Mardhiani ◽  
Deny Puriyani Azhari ◽  
Silviana Wulansari
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Room Temperature ◽  
Antioxidant Activities ◽  
Skin Barrier ◽  
Penetration Enhancer ◽  
Isopropyl Myristate ◽  
Topical Formulation ◽  
Diffusion Test ◽  
Active Substances

As a type of cosmetic preparation products, cream dosage form is widely used with the addition of active substances having antioxidant activities, such as vitamin C and its derivatives. Sodium ascorbyl phosphate (SAP) can be used in topical formulation due to its more stable properties than ascorbic acid. However, it is difficult to deliver SAP into the dermis in a suficient dose. To overcome the problem, occasionally we can add a penetration enhancer. In some literature, emollients that often added in cosmetic preparations also have another effect as a penetration enhancer. The purpose of this research was to observe wether emollient addition could influence the penetration of SAP in the cream formulation or not. SAP was formulated into four formulations with three different emollients: dimethicone (F1), capric triglyceride (F2), and isopropyl myristate (F3) and a formulation without the addition of emollients (F4). The diffusion test was performed by Franz's diffusion cell method using male wistar rat’s abdominal membrane as a standard model of the skin barrier. The result of stability test showed that SAP cream was stable at room temperature but unstable on freeze thaw condition described by significant different values for all formulas. Nonetheless, the diffusion test showed that F2 with the capric triglyceride as emollient had the highest ability to pass SAP through the membrane, followed by isopropyl miristate. We concluded that emollient addition could influence the penetration of the cream of SAP.Keywords: vitamin c, ascorbic acid, sodium ascorbyl phospate, emollient, penetration enhancer

ASCORBIC ACID AND CAROTENE VALUES OF NATIVE EASTERN ARCTIC PLANTS

1967 ◽  
Vol 45 (10) ◽  
pp. 1859-1862 ◽  
Author(s):  
I. Hoffman ◽  
F. S. Nowosad ◽  
W. J. Cody
Keyword(s):  
Ascorbic Acid ◽  
Vitamin A ◽  
Vitamin C ◽  
Natural Resource ◽  
Native Plants ◽  
Wild Plants ◽  
Far North ◽  
Arctic Plants ◽  
Set Up ◽  
Β Carotene

Leaves, stems, roots, and berries of a number of native plants which were collected in the Ungava Bay region of northern Quebec were analyzed for ascorbic acid (vitamin C) and β-carotene (vitamin A) in a laboratory set up near Fort Chimo.The results indicated that some plants from the Eastern Arctic were remarkably vitamin-rich. These findings demonstrated that wild plants represent a valuable natural resource which might be utilized under survival conditions or as required to ensure proper nutrition in the far North.

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