scholarly journals Comparative Study of the Antioxidant Activity of Holarrhena Floribunda and Picralima Nitida

2021 ◽  
Vol 9 (10) ◽  
pp. 1925-1930
Author(s):  
Alida Edwige Odoh ◽  
Désirée Mariette Yéhé ◽  
Yao Kanga ◽  
Guédé-Noël Zirihi ◽  
Diénéba Koné-Bamba
Keyword(s):  
Antioxidant Activity ◽  
Comparative Evaluation ◽  
Dry Matter ◽  
Ferric Ion ◽  
Total Phenols ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Leaf Extracts ◽  
Tract Infections ◽  
Activity Quantification

Picralima nitida (Stapf) T.Durand & H.Durand and Holarrhena floribunda (G.Don) T.Durand & Schinz are West and Central African plant species belonging to the Apocynaceae family. These two plants are used in traditional Ivorian medicine to treat hypertension, urinary tract infections, diarrhea, gonorrhea, malaria and diabetes. Phytochemical screening and evaluation of antioxidant activity of P. nitida fruit and H. floribunda leaf extracts of each of these two species have already been performed. In the present study, a comparative evaluation of the antioxidant activity of fruit (P. nitida) and leaf (H. floribunda) decoctions was carried out. The decoctions of P. nitida fruits and H. floribunda leaves were rich in secondary metabolites, especially polyphenols which have good antioxidant activity. Quantification of total phenols and flavonoids gave respective values of QP = 15235.632 ± 622 µg GAE / g dry matter and FP = 2.387 ± 0.387% for P. nitida and QH = 68597.701 ± 3171 µg GAE / g dry matter and FH = 17.581 ± 0.379% for H. floribunda. P. nitida showed antioxidant activity against DPPH radical (IC50 = 104.30 ± 3.17 μg / mL) and ferric ion Fe3 + (261.4 ± 36. 87 μmoL Eq Trolox / g extract). H. floribunda showed antioxidant activity against DPPH radical (IC50 = 41.73 ± 0.29 μg / mL) and ferric ion Fe3 + (365 ± 20.36 μmol Eq Trolox / g ExS).

scholarly journals PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF MILLINGTONIA HORTENSIS (L)

2017 ◽  
pp. 162-167
Author(s):  
Janaki A. ◽  
Kaleena P. K. ◽  
Elumalai D. ◽  
Hemalatha P. ◽  
Babu M. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Leaf Extracts ◽  
Micro Organisms ◽  
Dpph Radical Scavenging

Objective: Millingtonia hortensis Linn (Bignoniaceae) is commonly known as cork tree and Akash neem. Aim of studies to determine the antioxidant activity and antibacterial activity.Methods: The antioxidant activity of different solvent extracts were measured by chemical analyses involving the assay of 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and super oxide radical scavenging activity.Results: Phytochemicals (secondary metabolites) screening of methanol, chloroform, ethanol, petroleum ether, aqueous leaf extracts revealed the presence of carbohydrates, tannins, saponins, flavonoids, alkaloids, betacyanins, phenols and coumarins.Conclusion: The presence of these phytochemicals and antioxidant capacity support the use of this plant as an antibacterial agent against the group of micro organisms tested. 

scholarly journals Phytochemical Screening and Assessment of Antibacterial, Antioxidant and Antihelminthic Activities of Sarcocephalus latifolius Leaves

2021 ◽  
Vol 3 (4) ◽  
pp. 89-95
Author(s):  
Jeff B. Iteku ◽  
Ornella V. Makaya ◽  
Samy Ngunde-te-Ngunde ◽  
Clément L. Inkoto ◽  
Emmanuel M. Lengbiye ◽  
...  
Keyword(s):  
Antioxidant Status ◽  
Dry Matter ◽  
Mineral Elements ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Total Polyphenols ◽  
Antioxidant Power

The main objective of our study was to determine the cheminical composition and to assess the antioxidant, antibacterial and antihelminthic activities of the leaves extracts of S. latifolius. The antibacterial activity of S. latifolius leaves extracts was assessed using micro-dilution method. In end, the antioxidant capacity of different plant extracts has been evaluated by determining their ability to reduce iron (III) to iron (II) and DPPH radical. The results obtained revealed that the dry matter of the powder of the S. latifolius leaves is rich in fibers, crude proteins, lipids and carbohydrates. Its water content was moderate (14.3 %); the leaves of S. latifolius are rich in mineral elements like calcium (Ca), magnesium (Mg), potassium (K) and iron (Fe); the plant has the main groups of secondary metabolites such as total polyphenols, flavonoids, tannins, alkaloids, bound and free quinones, steroids and tri-terpenes, while it is poor in anthocyanins, leucoanthocyans and saponins. The bacterial strains used were less sensitive to S. latifolius leaf extracts (MIC greater than or equal to 500 µg/mL). The extracts of this plant showed significant antioxidant and antihelmintic activity, with a strong correlation between the methods used to evaluate the antioxidant potential of DPPH and FRAP. Due to their interesting functional properties, this plant could be used in the formulation of a nutraceutical with strong antioxidant power to improve the antioxidant status of consumers, but also in the management of helminthiases.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Phenolic Content and Antioxidant Activity in Trifolium Germplasm from Different Environments

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 298 ◽  
Author(s):  
Aldo Tava ◽  
Łukasz Pecio ◽  
Roberto Lo Scalzo ◽  
Anna Stochmal ◽  
Luciano Pecetti
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Chemical Structure ◽  
Dry Matter ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Adaptive Strategies ◽  
Phenolic Composition ◽  
Leaf Extracts ◽  
Plant Environment

Phenolics are important mediators in plant-environment interactions. The presence and concentration of phenolic compounds and their antioxidant activity were evaluated in leaves and flowers of a set of Trifolium species originating from contrasting environments encompassing lowland and mountain sites. The current germplasm proved a great reservoir of phenolic compounds, with different chemical structure and, possibly, diversified biological activity. Germplasm groups with specific phenolic composition were observed. In some cases, different patterns bore a taxonomic meaning. Lowland germplasm showed higher concentration of total phenolics in leaves than mountain accessions (50.30 vs. 34.19 mg/g dry matter (DM)), while the latter had higher concentration in flowers (114.16 vs. 57.44 mg/g DM). Outstanding concentration of isoflavones was observed in leaves of lowland germplasm (24.19 mg/g DM), and of both proanthocyanidins and flavonoids in flowers of mountain germplasm (53.81 and 56.62 mg/g DM, respectively). The pattern of phenolic composition in lowland and mountain germplasm was suggestive of different adaptive strategies. Three assays of antioxidant activity were tested, which were characterised by rather different reactivity towards phenolic composition. The scavenging activity was higher for leaf extracts of lowland germplasm, and for flower extracts of mountain germplasm. Besides identifying germplasm of interest, this study also suggested possible links between environmental factors and concentration and composition of phenolic compounds.

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

TOTAL PHENOLS CONTENT AND ANTIOXIDANT ACTIVITY OF DIFFERENT POLARITY CRUDE EXTRACTS OF DODONAEA VISCOSA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (08) ◽  
pp. 79-83
Author(s):  
Maisa R. Shoriqi ◽  
Salem S. Touby ◽  
Mohammad A. Hossain ◽  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Total Phenols ◽  
Dodonaea Viscosa ◽  
Leaf Extracts ◽  
Coarse Powder ◽  
Dry Extract ◽  
Crude Extracts ◽  
Antioxidant Agents

Nowadays, effective medications as antioxidant agents are mandatory for a safe and sustainable environment. Dodonaea viscosa (D. viscosa) is used traditionally by the Omani people to treat rheumatism, toothaches, fever, cold, malaria, headaches, indigestion, ulcers, diarrhea, and constipation, dysmenorrheal and irregular menstruation. This study was carried out to prepare leaf extracts by different solvents and to determine their antioxidant activity and total phenols content. The selected plant was collected locally near the University Campus, Nizwa, Oman. The dried coarse powder was used for the extraction with methanol and it was defatted with water and successively partitioned with different polarity solvents with increasing solvent polarity. The total phenols content and antioxidant activity of the prepared different extracts were assessed by Folin-Ciocalteu reagent (FCR) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) methods. The total phenols content of different extracts was in the range of 3.02-249.93 mg gallic acid/g dry extract. The chloroform extract showed the maximum amount of total phenol compounds (249.93 mg GAE acid/g dry extract) and the minimum content was found in water extract (3.02 mg GAE acid/g dry extract). The leaf crude extracts were obtained to significant levels of antioxidant activity that ranged from percentage of inhibition from 33-85.92 %. The water extract and n-butanol extracts showed significant levels of antioxidant activity (85.92 % and 84.99 %) against the DPPH free radical method. In conclusion, this study showed that different polarities crude extracts of D. viscosa comprise a significant amount of phenols plus antioxidant properties and have possibilities of being potential use of the selected species for a natural source of antioxidants.

scholarly journals Study of Antioxidant Effects of Selected Types of Coffee

2016 ◽  
Vol 60 (3) ◽  
pp. 34-38 ◽  
Author(s):  
J. Hudáková ◽  
D. Marcinčáková ◽  
J. Legáth
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Green Coffee ◽  
Total Phenols ◽  
Dpph Radical ◽  
The Individual ◽  
Dpph Radical Scavenging ◽  
Antioxidant Effects

AbstractCoffee is a rich source of dietary antioxidants which protects the human body against the effects of dangerous free radicals. The aim of this study was to determine and compare the antioxidant activity, content of total phenols and flavonoids in selected types of coffee with respect to the way of their processing. The individual coffees were investigated with regard to their origin and composition. The antioxidant effects were determined by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay. The content of total phenols was analysed by the Folin-Ciocalteu method and the content of flavonoids in the coffee extracts was determined by a colorimetric method. The highest antioxidant activity was exhibited by the extract of unroasted ground 100 % green coffeeArabica(89.55 %), and the high scavenging of free radicals was achieved also by the extracts of roasted ground 100 % coffeesArabicaandRobusta. The highest levels of total phenols (77.54 µg.ml−1) and flavonoids (1.74 µg.ml−1) were measured in the extract of unroasted ground 100 % green coffeeArabica. High levels of these substances were found also in extracts of roasted ground 100 % coffeesArabicaandRobusta. The lowest levels of total phenols (31.24 µg.ml−1) and flavonoids (0.91 µg.ml−1) were detected in the extract of instant coffeeArabica. The processing of coffee by roasting decreased the level of the investigated antioxidant components but considerably improved the taste and aroma, the properties that make coffee one of the most popular drinks in the world.

scholarly journals PHYTOCHEMICAL STUDY AND IN VITRO ANTIOXIDANT ACTIVITIES OF HAMMADA SCOPARIA EXTRACTS FROM SOUTHEASTERN ALGERIA

2018 ◽  
Vol 11 (10) ◽  
pp. 187
Author(s):  
Benkherara Salah ◽  
Bordjiba O
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Condensed Tannins ◽  
Aerial Part ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Phytochemical Screening ◽  
Trolox Equivalent

Objective: This study was carried out to determine the phytochemical constituents and to evaluate the antioxidant potential of the aerial part extracts of Hammada scoparia (Pomel) Iljin to validate the medicinal potential of this Algerian plant species.Methods: Crude extracts were prepared by cold maceration with absolute methanol and distilled water. Preliminary phytochemical screening is carried out to detect the presence of the major secondary metabolites using qualitative characterization methods. Quantitative estimation of total phenols, total flavonoids, flavanols, flavonols, and condensed tannins contents is performed using gallic acid, rutin, catechin, and quercetin as standards. In vitro antioxidant activity was evaluated by the free radical scavenging activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity by scavenging of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay, and the ferric reducing power assay (ferric reducing antioxidant power).Results: Phytochemical screening showed the presence of alkaloids, flavonoids, tannins, terpenes and sterols, saponins, and anthocyanins. Total phenols were present more in aqueous crude extract (ACE) with 336.756±0.855 mg gallic acid equivalent/g DM. Total flavonoids and flavonols were more abundant in methanolic crude extract (MCE) than ACE. However, condensed tannins and flavanols were present less in MCE with only 0.958±0.052 and 4.547±0.055 mg CE/g DM, respectively. The ACE of this plant species had greater antioxidant activity than the other extract by DPPH and ABTS assays (35.823±0.129 and 51.323±0.394 mg trolox equivalent/g DM). The better ferric reducing power (2060.535±2.566 mM Fe+2/g DM) was also recorded with the same extract.Conclusion: ACE of aerial part of H. scoparia (Pomel) Iljin showed a high amount of secondary metabolites. The obtained results confirmed that the extracting solvent influenced the antioxidant property estimations of this plant. Hence, the ACE can be further exploited further for in vitro and in vivo research work.

scholarly journals COMPARATIVE ANALYSIS OF PHYTOCHEMICAL, ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF AZADIRACHTA INDICA LEAVES

2019 ◽  
pp. 151-154
Author(s):  
GAURAV SHARMA ◽  
ANKITA THAKUR ◽  
SOHAN LAL ◽  
ROHIT KUMAR NADDA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Comparative Evaluation ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Objective: The objective of the present study was the analysis of phytochemicals in various extracts of Azadirachta indica leaves, comparative evaluation of antibacterial activity of the various extracts of A. indica leaves against Escherichia coli and Staphylococcus aureus, and comparative evaluation of antioxidant activity in various extracts of A. indica leaves using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Methods: Various extracts were prepared by crushing the samples. Antibacterial susceptibility test, various phytochemical tests for qualitative analysis, and DPPH radical scavenging assay for antioxidant activity were performed. Results: The result suggested that alkaloids, flavonoids, and terpenoids were present in all the four extracts. Tannins were absent in the ethyl acetate extract, and phenols were only present in the ethyl acetate extract. Sterols and phlobatannins were absent in all the four extracts. Saponins were only present in the aqueous extract, and amino acids were only present in the ethyl acetate extract. The bacterial strains S. aureus and E. coli were used against the different extracts of A. indica leaves, i.e., methanol, chloroform, ethyl acetate, and aqueous. Conclusion: The results suggested that bioactive compounds found in leaves of A. indica contribute to its pharmacological activities.

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