scholarly journals Preparation, Characterization of Silver-Nanoparticles from Seed Coat Exudates of Celastrus paniculatus Willd. and Their Bactericidal and Biofilm Inhibition Effects

2019 ◽  
Vol 11 (05) ◽  
Author(s):  
Anil Kumar Moola1 ◽  
Sundararajan Balasubramani ◽  
Ranjitha Kumari Bollipo Diana1 ◽  
Chari Nithya
Keyword(s):  
Silver Nanoparticles ◽  
Seed Coat ◽  
Skin Diseases ◽  
Screening Method ◽  
Bioactive Molecules ◽  
Metallic State ◽  
Face Centered Cubic ◽  
Biofilm Inhibition ◽  
Vis Spectroscopy ◽  
Celastrus Paniculatus

We are reporting a novel approach for preparing silver nanoparticles with seed coat exudates of Celastrus paniculatus Willd, a medicinal plant traditionally used for the treatment of skin diseases, fever, leprosy and neurological disorders. HPTLC study revealed that aqueous, soluble seed coat exudates contain phenolics, alakaloids and flavonoids. The formation, crystalline nature and morphology of the nanoparticles were identified by UV–Vis spectroscopy, X-ray Diffraction (XRD) analysis, Transmission Electron Microscopy (TEM) and selected-area electron diffraction (SAED). Functional group stretching of aqueous soluble extracts was identified by using FTIR. Results revealed that nanosilver particles are spherical, range in size from 10 to 82 nm and crystalize in face-centered cubic structures. Surface-enhanced Raman spectra analysis showed that AgNP are capped with bioactive molecules from exudates and that they may act as precursors of the reduction of silver nitrate from the metallic state (Ag+) to the atomic state (Ag0). We also examined the minimal inhibitory concentration for bacteria Escherichia coli and Bacillus subtilis using a resazurin color assay. Nanosilver strongly inhibited the bacterial growth, leading to MIC values of 40μg/ml and 60μg/ml for the bacteria, respectively. The colony screening method and inhibition kinetics of biofilm formation in the Klebsiella pneumoniae strain were also studied using the tube method and a quantitative microplate assay. SEM analysis and quantification of the EPS revealed a fivefold decrease in concentration in treated compared to untreated. The inhibition response was duly reflected in SEM images.

scholarly journals Green Synthesis and Characterization of Pullulan Mediated Silver Nanoparticles through Ultraviolet Irradiation

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2382 ◽  
Author(s):  
Muhammad Jamshed Khan ◽  
Suriya Kumari ◽  
Kamyar Shameli ◽  
Jinap Selamat ◽  
Awis Qurni Sazili
Keyword(s):  
Silver Nanoparticles ◽  
Uv Irradiation ◽  
Irradiation Time ◽  
Edible Films ◽  
Crystallographic Structure ◽  
Face Centered Cubic ◽  
Selected Area Electron Diffraction ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Lattice

Nanoparticles (NPs) are, frequently, being utilized in multi-dimensional enterprises. Silver nanoparticles (AgNPs) have attracted researchers in the last decade due to their exceptional efficacy at very low volume and stability at higher temperatures. Due to certain limitations of the chemical method of synthesis, AgNPs can be obtained by physical methods including sun rays, microwaves and ultraviolet (UV) radiation. In the current study, the synthesis of pullulan mediated silver nanoparticles (P-AgNPs) was achieved through ultraviolet (UV) irradiation, with a wavelength of 365 nm, for 96 h. P-AgNPs were formed after 24 h of UV-irradiation time and expressed spectra maxima as 415 nm, after 96 h, in UV-vis spectroscopy. The crystallographic structure was “face centered cubic (fcc)” as confirmed by powder X-ray diffraction (PXRD). Furthermore, high resolution transmission electron microscopy (HRTEM) proved that P-AgNPs were covered with a thin layer of pullulan, with a mean crystalline size of 6.02 ± 2.37. The average lattice fringe spacing of nanoparticles was confirmed as 0.235 nm with quasi-spherical characteristics, by selected area electron diffraction (SAED) analysis. These green synthesized P-AgNPs can be utilized efficiently, as an active food and meat preservative, when incorporated into the edible films.

scholarly journals Green Synthesis and Catalytic Activity of Silver Nanoparticles Based on Piper chaba Stem Extracts

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1777 ◽  
Author(s):  
Md. Mahiuddin ◽  
Prianka Saha ◽  
Bungo Ochiai
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ftir Spectroscopy ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Size

A green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet–visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs effectively catalyzed the reduction of 4-nitrophenol (4-NP) and degradation of methylene blue (MB) in the presence of sodium borohydride.

scholarly journals Green Synthesis of Silver Nanoparticles in the Presence of Polysaccharide: Optimization and Characterization

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Margarita I. Skiba ◽  
Victoria I. Vorobyova ◽  
Alexander Pivovarov ◽  
Natalya P. Makarshenko
Keyword(s):  
Silver Nanoparticles ◽  
Sodium Alginate ◽  
Xrd Analysis ◽  
Face Centered Cubic ◽  
Colloidal Solutions ◽  
Ag Nps ◽  
Sem Images ◽  
Spherical Powder ◽  
Stabilizing Agent ◽  
Vis Spectroscopy

The process of obtaining aqueous solutions of silver nanoparticles with the use of a low-temperature nonequilibrium contact plasma and stabilizing agent—polysaccharide (sodium alginate)—has been examined. The synthesized Ag NPs were characterized by using UV-Vis spectroscopy, dynamic light scattering (DLS), scanning electron microscope (SEM), and XRD analysis. The effect of concentration of Ag+, sodium alginate, duration of processing by plasma discharge, and pH of liquid on the production of silver nanoparticles has been studied. The results demonstrated that synthesis provides the formation of silver nanoparticles for investigated concentrations of Ag+ (0.3-3.0 mmol/l) and 5.0 g/l Na-Alg (pH=7–10) within 1–5 minutes. From the SEM images, the silver nanoparticles are found to be almost spherical. Powder XRD results reveal that Ag nanoparticles have a face-centered cubic crystal structure. Zeta potential of plasma-chemically obtained colloidal solutions at various concentrations of Ag+ ions and stabilizing agent varies from −32.8 to −39.3 mV, indicating the moderate stability of synthesized nanoparticles.

scholarly journals Sustainable phyto-fabrication of silver nanoparticles using Gmelina arborea exhibit antimicrobial and biofilm inhibition activity

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Smitha Chandrasekharan ◽  
Gandhimathi Chinnasamy ◽  
Somika Bhatnagar
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Activity Level ◽  
Health Concern ◽  
Inhibition Activity ◽  
Gmelina Arborea ◽  
Biofilm Inhibition ◽  
Vis Spectroscopy

AbstractIncrease in bacterial resistance to commonly used antibiotics is a major public health concern generating interest in novel antibacterial treatments. Aim of this scientific endeavor was to find an alternative efficient antibacterial agent from non-conventional plant source for human health applications. We used an eco-friendly approach for phyto-fabrication of silver nanoparticles (AgNPs) by utilizing logging residue from timber trees Gmelina arborea (GA). GC–MS analysis of leaves, barks, flowers, fruits, and roots was conducted to determine the bioactive compounds. Biosynthesis, morphological and structural characterization of GA-AgNPs were undertaken by UV–Vis spectroscopy, scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDX), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometer (XRD). GA-AgNPs were evaluated for antibacterial, antibiofilm, antioxidant, wound healing properties and their toxicity studies were carried out. Results identified the presence of terpenoids, sterols, aliphatic alcohols, aldehydes, and flavonoids in leaves, making leaf extract the ideal choice for phyto-fabrication of silver nanoparticles. The synthesis of GA-AgNPs was confirmed by dark brown colored colloidal solution and spectral absorption peak at 420 nm. Spherical, uniformly dispersed, crystalline GA-AgNPs were 34–40 nm in diameter and stable in solutions at room temperature. Functional groups attributed to the presence of flavonoids, terpenoids, and phenols that acted as reducing and capping agents. Antibacterial potency was confirmed against pathogenic bacteria Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus by disc diffusion assay, MIC and MBC assay, biofilm inhibition assay, electron-microscopy, cell staining and colony counting techniques. The results from zone of inhibition, number of ruptured cells and dead-cell-count analysis confirmed that GA-AgNPs were more effective than GA-extract and their bacteria inhibition activity level increased further when loaded on hydrogel as GA-AgNPs-PF127, making it a novel distinguishing feature. Antioxidant activity was confirmed by the free radical scavenging assays (DPPH and ABTS). Wound healing potential was confirmed by cell scratch assay in human dermal fibroblast cell lines. Cell-proliferation study in human chang liver cell lines and optical microscopic observations confirmed non-toxicity of GA-AgNPs at low doses. Our study concluded that biosynthesized GA-AgNPs had enhanced antibacterial, antibiofilm, antioxidant, and wound healing properties.

Green Synthesis of Silver Nanoparticles using the Leaves Extract of Filicium decipiens and its Anti- Microbial Activity

2021 ◽  
Vol 10 (3) ◽  
pp. 16-24
Author(s):  
Sherin Monichan ◽  
P. Mosae Selvakumar ◽  
Christine Thevamithra ◽  
M. S. A. Muthukumar Nadar ◽  
Jesse Joel
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Average Diameter ◽  
Diffusion Method ◽  
Face Centered Cubic ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Almost All

Silver nanoparticles has been used since ages, even till now it is exploited in almost all areas like medicine, textiles, industries, cosmetics, purification, dying and many more. There are many approaches which are used to synthesize silver nanoparticles. However, these approaches are either harmful to the environment or very costly. Therefore, green synthesis of silver nanoparticles (AgNPs) using leaves of Filicium decipiens eco-friendly and a very reliable method to procure AgNPs. Characterization of synthesized AgNPs were then done using UV-Vis spectroscopy and fluorescence which confirmed the formation of AgNPs, scanning electron microscope (SEM)confirmed its shape to be round and X-ray diffraction (XRD) determined its crystalline nature as face centered cubic structure. Furthermore, Dynamic Light Scattering (DLS) was also done in order to know the average diameter and zeta potential of AgNPs. However, it did not show potential results due to the aggregates formed during the green synthesis of AgNPs. In addition to this, anti-microbial test against bacteria such as gram negative (Escherichia. Coli) and gram positive (Bacillus.spc) were done using well-diffusion method and also its application of antimicrobial activity was tested over fabric to understand its application in textile industries. In both the cases, AgNPs showed more efficiency in gram negative bacteria than gram- positive.

scholarly journals Synthesis, Characterization, and Evaluation of the Antibacterial Activity ofAllophylus serratusLeaf and Leaf Derived Callus Extracts Mediated Silver Nanoparticles

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Kero Jemal ◽  
B. V. Sandeep ◽  
Sudhakar Pola
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
Eds Analysis ◽  
Vis Spectroscopy ◽  
Absorbance Peak ◽  
Face Centered ◽  
Spectroscopy Studies

Allophylus serratusmediated silver nanoparticles biosynthesis, characterization, and antimicrobial activity were described. The synthesis of silver nanoparticles was confirmed by visual observation: UV-Vis spectrum, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), and Fourier Transform Infra-Red (FTIR). UV-Vis spectroscopy studies showed that the absorption spectra of synthesized silver nanoparticles from leaf and callus extracts had absorbance peak range of 440 nm and 445 nm, respectively. The X-RD pattern revealed the presence of crystalline, dominantly spherical silver nanoparticles in the sample having size ranging from 42 to 50 nm. The XRD peaks 38.2°, 44.1°, 64.1°, and 77.0° for leaf extract and 38.1°, 44.3°, 64.5°, 77.5°, and 81.33° for callus extract can be assigned the plane of silver crystals (111), (200), (220), and (311), respectively, and indicate that the silver nanoparticles are face-centered, cubic, and crystalline in nature. SEM and EDS analysis also confirmed the presence of silver nanoparticles. The FTIR results showed the presence of some biomolecules in extracts that act as reducing and capping agent for silver nanoparticles biosynthesis. The synthesized silver nanoparticles showed significant antibacterial activity againstKlebsiella pneumoniaeandPseudomonas aeruginosa.

scholarly journals Sustainable Synthesis of Silver Nanoparticles Using Marine Algae for Catalytic Degradation of Methylene Blue

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1377
Author(s):  
Chandra Kishore Somasundaram ◽  
Raji Atchudan ◽  
Thomas Nesakumar Jebakumar Immanuel Edison ◽  
Suguna Perumal ◽  
Rajangam Vinodh ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Methylene Blue ◽  
Silver Nanoparticles ◽  
Marine Algae ◽  
Dye Degradation ◽  
Catalytic Degradation ◽  
Attenuated Total Reflection ◽  
Face Centered Cubic ◽  
X Ray ◽  
Vis Spectroscopy

Herein, Sargassum coreanum (marine algae)-mediated silver nanoparticles (AgNPs) were successfully synthesized by a simple reduction method. The synthesized AgNPs were characterized using ultraviolet-visible spectroscopy, attenuated total reflection Fourier transformed infrared spectroscopy, X-ray diffractometry, field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray (EDX) spectroscopy, and high-resolution transmission electron microscopy (HR-TEM) analysis. The acquired colloidal AgNPs were strongly absorbed around 420 nm and displayed brown color under visible light. The XRD pattern of AgNPs exposed their face-centered cubic geometry along with crystalline nature. The HRTEM images of synthesized AgNPs confirmed the mean particle size of 19 nm with a distorted spherical shape, and the calculated interlayer distance (d-spacing value) was about 0.24 nm. Further, the catalytic degradation of methylene blue using sodium borohydride and AgNPs was monitored using UV–vis spectroscopy. The result revealed that AgNPs performed as a superior catalyst, which completely degraded MB in 20 min. The rate constant for MB degradation was calculated to be 0.106 min−1, demonstrating that the marine algae-mediated AgNPs had outstanding catalytic activity. This approach is easy and environmentally benign, which can be applied for environmental-based applications such as dye degradation and pollutant detoxification.

scholarly journals Biosynthesis of Silver Nanoparticles Using Ligustrum Ovalifolium Fruits and Their Cytotoxic Effects

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 627 ◽  
Author(s):  
Bianca Moldovan ◽  
Vladislav Sincari ◽  
Maria Perde-Schrepler ◽  
Luminita David
Keyword(s):  
Silver Nanoparticles ◽  
Synthesis Method ◽  
Cost Effective ◽  
Bioactive Molecules ◽  
Energetic Cost ◽  
Fruit Extract ◽  
Vis Spectroscopy ◽  
Biosynthesized Agnps ◽  
Average Size

The present study reports for the first time the efficacy of bioactive compounds from Ligustrum ovalifolium L. fruit extract as reducing and capping agents of silver nanoparticles (AgNPs), developing a green, zero energetic, cost effective and simple synthesis method of AgNPs. The obtained nanoparticles were characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR), confirming that nanoparticles were crystalline in nature, spherical in shape, with an average size of 7 nm. The FTIR spectroscopy analysis demonstrated that the AgNPs were capped and stabilized by bioactive molecules from the fruit extract. The cytotoxicity of the biosynthesized AgNPs was in vitro evaluated against ovarian carcinoma cells and there were found to be effective at low concentration levels.

scholarly journals Green Synthesis of Silver Nanoparticles using Medicinal Plants BerberisAsiatica and Cassia Fistula and Evaluation of Antioxidant and Anti-bacterial Activities

2021 ◽  
Vol 19 (2) ◽  
pp. 25-32
Author(s):  
Deegendra Khadka ◽  
Rachana Regmi ◽  
Mitesh Shrestha ◽  
Megha Raj Banjara
Keyword(s):  
Silver Nanoparticles ◽  
Nanoparticle Synthesis ◽  
Cost Effective ◽  
Face Centered Cubic ◽  
Related Field ◽  
Cost Effective Method ◽  
Vis Spectroscopy ◽  
Health Related ◽  
Face Centered ◽  
Biosynthesized Agnps

The application of silver nanoparticles in various sectors including health related field is remarkably profound. Nowadays, the research of synthesizing metal nanoparticles (MNPs) using plant extracts is fascinating field as it offers the eco-friendly and cost-effective method for nanoparticle synthesis. In this study, we synthesized silver nanoparticles (AgNPs) using methanolic extract of B.asiatica and C. fistula regarding their ethnomedical importance. The synthesized AgNPs were characterized by UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometer (XRD). UV-vis spectroscopy exhibited the characteristic Surface Plasmon Peak of silver nanoparticle~420 nm.FTIR data were measured to get a preliminary idea on the functional groups responsible for the stabilization of AgNPs. XRD data confirmed the natural crystal structure with a face centered cubic of AgNPs. The antibacterial activity of biosynthesized AgNPs was assessed by testing promptly available gram-positive Staphylococcus aureus and gram-negative Escherichia coli bacterial strain and antioxidant activity was calculated by DPPH assay. The overall outcomes of the studies concluded that the application of the biogenic synthesis of AgNPs of B. asiaticaas an antioxidant and antibacterial agent is more potent showing IC50 value 65.1±1.30 μg/mL and the highest zone of inhibition 15 mm in diameter against S. aureus.

scholarly journals BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM MARINE MACROSCOPIC RED SEAWEED HALYMENIA PORPHYROIDES BOERGESEN (CRYPTON) AND ITS ANTIFUNGAL EFFICACY AGAINST DERMATOPHYTIC AND NON-DERMATOPHYTIC FUNGI

2020 ◽  
pp. 174-181
Author(s):  
VISHNU KIRAN MANAM ◽  
MURUGESAN SUBBAIAH
Keyword(s):  
Silver Nanoparticles ◽  
Tamil Nadu ◽  
Synthesis Method ◽  
Diffusion Method ◽  
Face Centered Cubic ◽  
Red Seaweed ◽  
Vis Spectroscopy ◽  
Dermatophytic Fungi ◽  
Ft Ir ◽  
Antifungal Efficacy

Objective: The current study illustrates the biosynthesis of economically scalable and energy efficient colloidal silver nanoparticles (AgNPs) from marine red seaweed Halymenia porphyroides Boergesen (Crypton) collected from Southeast coast of Tamil Nadu, India, and their antifungal efficacy against dermatophytic and non-dermatophytic fungi was evaluated. Methods: The biosynthesis of silver nanoparticles from marine macroscopic red seaweed H. porphyroides Boergesen were synthesized by green synthesis method and characterized by UV–Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction, thermogravimetric analysis (TGA), scanning electron microscope (SEM), and transmission electron microscopy (TEM). The efficacy of silver nanoparticles against dermatophytic and non-dermatophytic fungi was performed by disk diffusion method. Results: The presence of silver nanoparticles with an average size between 34.3 and 80 nm and exhibiting face-centered cubic structure was confirmed. SEM revealed the morphology of the nanoparticles as spherical and TEM exhibited the nanoparticle distribution. The FT-IR spectra confirmed the presence of potential biomolecules in the seaweed crude extract which is responsible for reducing and capping the bioreduced silver nanoparticles. The UV absorption spectra at 430.5 nm revealed the characteristic spectra of the silver nanoparticles. The purity and the thermal stability of silver nanoparticles were revealed by TGA. Silver nanoparticles showed significant efficacy against dermatophytes and Rhizopus microsporus among non-dermatophytes. Conclusion: Intermediate efficacy was observed against dermatophytes and among non-dermatophytic fungi R. microsporus exhibited better efficacy, whereas Aspergillus flavus were resistant to the biosynthesized silver nanoparticle.

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