scholarly journals Evaluation of phytochemical contents of Emilia coccinea leaves

2017 ◽  
Vol 1 ◽  
pp. 47
Author(s):  
Ajah Obinna ◽  
Unegbu C Chika ◽  
Anyanwu O Ogechi ◽  
Amaralam Eric Chinedu

Emilia coccinea is widely used in folkloric medicine for eye and ear ailments as well as for fever. This present study evaluated the preliminary and quantitative phytochemical properties of E. coccinea leaves using standard procedures. The results revealed the following bioactive compounds Flavonoids (0.90 ± 0.02), Alkaloids (0.94 ± 0.03), Tannins (10.36 ± 0.02), Saponins (2.34 ± 0.02), Oxalate (1.62 ± 0.01), Phenols (0.89 ± 0.02), Terpenoids (0.11 ± 0.01). The high concentration of tannin and moderate concentration of other phytochemical proved that E. coccinea can serve as a vital medicinal plant that could be used for pharmaceutical formulations. 

2020 ◽  
pp. 173-192
Author(s):  
Paul Oluwatimilehin Ogunbamowo ◽  
Musbau Bayonle Olaniyi ◽  
Olamilekan Lanre Awotedu ◽  
Ibraheem Oduola Lawal

Justicia secunda es una planta medicinal que pertenece a la familia Acanthaceae, es muy utilizada en la medicina tradicional para el tratamiento y manejo de una amplia gama de dolencias. En este estudio, la selección/análisis de fitoquímicos, minerales y características micro-morfológicas de las hojas de J. secunda se evaluaron utilizando procedimientos estándar. Los datos generados para el contenido fitomineral y fitoquímico de las hojas así como la prueba quimio-microscópica y los rasgos anatómicos foliares de las hojas que revelaron rasgos característicos distintos serán de utilidad en la sistemática, adecuada identificación, autenticación y estandarización de esta planta para fines medicinales propósitos. Justicia secunda is a medicinal plant that belongs to the family Acanthaceae, it is widely used in traditional medicine for the treatment and management of a wide range of ailments. In this study, the phytochemicals screening/analysis, minerals and micro-morphological characteristics of the leaves of J. secunda were evaluated using standard procedures. The data generated for the phytomineral and phytochemical contents of the leaves as well as the chemo-microscopic test and foliar anatomical features of the leaves which revealed distinct characteristic features will be useful in the systematics, proper identification, authentication and standardization of this plant for medicinal purposes.


Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1217
Author(s):  
Ganbolor Jargalsaikhan ◽  
Jin-Yi Wu ◽  
Yen-Chou Chen ◽  
Ling-Ling Yang ◽  
Ming-Shun Wu

The Mongolian rhubarb—Rheum undulatum L. (RU)—and Rumex crispus L. (RC)—a Taiwanese local rhubarb belonging to the family of Polygonaceae—are principal therapeutic materials in integrative medicine due to their rich quantities of bioactive compounds; however, their phytochemical and antioxidant properties, and anti-cancer activity is poorly investigated. Furthermore, the phytochemical characteristics of both species may be affected by their different geographical distribution and climatic variance. The current study aimed to compare RU with RC extracts in different polarity solvents (n-hexane, ethyl acetate, acetone, ethanol, and water) for their phytochemical contents including the total phenolic content (TPC), total anthraquinone content (TAC), total flavonoid content (TFC), antioxidant and free radical scavenging capacities, and anticancer ability on the HepG2 cell. Except for the n-hexane extract, all of the RU extracts had considerably higher TPCs than RC extracts, ranging from 8.39 to 11.16 mg gallic acid equivalent (GAE) per gram of dry weight, and the TPCs of each extract were also significantly correlated with their antioxidant capacities by ABTS, DPPH, and FRAP assays (p < 0.05). Moreover, there was no remarkable association between the antioxidant capacities and either TACs or TFCs in both the RU and RC extracts. Besides, high-performance liquid chromatography (HPLC) analysis revealed that both the RU and RC extracts contained chrysophanol, emodin, and physcion, and those bioactive compounds were relatively higher in the n-hexane solvent extracts. Additionally, we observed different levels of dose-dependent cytotoxic effects in all the extracts by cell viability assay. Notably, the ethanol extract of RU had a compelling cytotoxic effect with the lowest half-maximum inhibition concentration (IC50-171.94 ± 6.56 µg/mL at 48 h) among the RU extracts than the ethanol extract of RC. Interestingly, the ethanol extract of RU but not RC significantly induced apoptosis in the human liver cancer cell line, HepG2, with a distinct pattern in caspase-3 activation, resulting in increased PARP cleavage and DNA damage. In summary, Mongolian Rhubarb, RU, showed more phytochemical contents, as well as a higher antioxidant capacity and apoptotic effect to HepG2 than RC; thus, it can be exploited for the proper source of natural antioxidants and liver cancer treatment in further investigation.


Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 700
Author(s):  
Bianca R. Albuquerque ◽  
Maria Inês Dias ◽  
Carla Pereira ◽  
Jovana Petrović ◽  
Marina Soković ◽  
...  

Fruit bio-residues can be interesting for the recovery of bioactive molecules, such as phenolic compounds, tocopherols, vitamins, among others. These compounds can be targeted at the food industry and used for the development of functional foods or as food additives. In some cases, fruit epicarps are converted into by-products with non-commercial value, and generally, these fruit parts have a higher content in bioactive compounds than the fruit pulp. From this perspective, S. odorifera, a Brazilian fruit, has an inedible epicarp that could be explored to obtain biological compounds. Therefore, the aims of this study were to evaluate the chemical composition and the antioxidant, anti-proliferative, anti-inflammatory, and antimicrobial bioactivities of this by-product. S. odorifera epicarp showed a total of four organic acids, four phenolic compounds, highlighting the high concentration of anthocyanins (24 ± 1 mg/g dry weight (dw)) and high content of tocopherols (366 ± 2 mg/100 g dw). The hydroethanolic extract showed considerable antioxidant activity (EC50 values of 48.2 ± 0.5 and 27 ± 1 µg/mL for TBARS and OxHLIA assays, respectively), as also antibacterial and antifungal activities (minimal inhibitory concentrations (MICs) ≤ 2.2 mg/mL). The results obtained in this study suggest that Sicana odorifera epicarp represents a reliable option for the development of novel natural-based colorants with functional/bioactive proprieties.


2014 ◽  
Vol 23 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Dinesh Giri ◽  
Sushma Tamta

This protocol has been developed for somatic embryogenesis in Hedychium spicatum. Simultaneously, a method has also been developed for the production of synthetic seeds by using somatic embryos. Direct somatic embryos were developed on cotyledon explants of zygotic embryos on MS supplemented with high concentration of NAA (20.0 µM). Induction of secondary embryogenesis was best in 2,4-D supplemented medium fortified with activated charcoal. Germination of somatic embryos was enhanced by using GA3. Besides this, round and semi-hard beads of somatic embryos (synthetic seeds) could be produced by using 2% Na-alginate and 100 mM calcium chloride and more than 30% germination of synthetic seeds was achieved in MS. Well acclimated plants produced via somatic embryogenesis and/or synthetic seeds were transferred to field where more than 60% survived. This simple study enabled us to obtain a number of plantlets throughout the year each cycle requiring a short period of time. Besides propagation, this study provided an ex situ method for conservation of this vulnerable Himalayan species.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17506Plant Tissue Cult. & Biotech. 23(2): 147-155, 2013  (December)


2019 ◽  
Vol 9 (4-A) ◽  
pp. 373-375
Author(s):  
Anuradha Gauttam ◽  
Nakuleshwer Dutt Jasuja ◽  
Rakesh Kumar

Various traditional systems of medicine enlightened the importance of Indian plants to have a great medicinal value. The present study was aimed to evaluate the Primary Metabolites study of Vetiveria lawsonii, belong to Poaceae family. Extracts were prepared in methanol, ethanol by Soxhlet extraction. Quantitative extraction of preliminary phytochemicals investigation revealed the presence of Carbohydrates (Starch and Total Soluble Sugar), Lipid, Proteins, and Phenol by using UV spectrometer. Experimental medicinal plant Vetiveria lawsonii are showing high concentration of primary metabolites. Hence, we can conclude that the methanol and ethanol extracts of Vetiveria lawsonii was possess primary metabolites. Keywords: - Vetiveria lawsonii; Primary Metabolites.


Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3258 ◽  
Author(s):  
Durante ◽  
Bleve ◽  
Selvaggini ◽  
Veneziani ◽  
Servili ◽  
...  

Olive paste (OP) is a novel by-product of olive mill industry composed of water, olive pulp, and skin. Due to its richness in bioactive compounds, OP exploitation for human consumption has recently been proposed. Starter driven fermented OP is characterized by a well-balanced lipid profile, rich in mono and polyunsaturated fatty acids, and a very good oxidative stability due to the high concentration of fat-soluble antioxidants. These characteristics make OP particularly suitable as a functional ingredient for food/feed industry, as well as for the formulation of nutraceutical products. New types of taralli were produced by adding 20% of fermented OP from black olives (cv Cellina di Nardò and Leccino) to the dough. The levels of bioactive compounds (polyphenols, triterpenic acids, tocochromanols, and carotenoids), as well as the fatty acid profile, were monitored during 180 days of storage and compared with control taralli produced with the same flour without OP supplementation. Taralli enriched with fermented OP showed significantly higher levels of bioactive compounds than conventional ones. Furthermore, enriched taralli maintained a low amount of saturated fatty acids and high levels of polyphenols, triterpenic acids, tocochromanols, and carotenoids, compared to the initial value, up to about 90 days in the usual conditions of retailer shelves.


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