scholarly journals Comparative study for obtaining inulinase and invertase by yeasts

2021 ◽  
Vol 26 (5) ◽  
pp. 3002-3007
Author(s):  
MARIANA-GRATIELA SOARE (VLADU) ◽  
◽  
MARIA-MONICA PETRESCU ◽  
MIHAELA-CARMEN EREMIA
Keyword(s):  
Comparative Study ◽  
Food Industry ◽  
Invertase Activity ◽  
Industrial Applications ◽  
Culture Collection ◽  
Inoculum Size ◽  
Agitation Speed ◽  
Inulinase Activity ◽  
Microelement Composition ◽  
Data Variation

The aim of this comparative study was to obtain a model for production of inulinase and invertase by species Saccharomyces, Candida and Hansenula, strains from culture collection of INCDCF-ICCF, using submerged fermentation in a medium containing inulin as source of C. This model explained the data variation and the actual relationships between the parameters and responses. The dry biomass content as well as the production of inulinase and invertase in the bioprocess medium was influenced by inulin concentration and microelement composition. The main parameters for bioprocesses were: inoculum size 2% (v/v), pH 6, temperature 280 C and 220 rpm agitation speed. Following comparative study for production of extracellular inulinase (exo and endo inulinase) and invertase were obtained for Candida arborea the best results, invertase production having significantly higher concentrations than inulinase (35.92 U/mL invertase activity vs. 8.01 U/mL inulinase activity), on M5 medium. These results could be useful for industrial applications such as food industry, pharmaceutical.

scholarly journals The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry

2020 ◽  
Vol 15 (1) ◽  
pp. 787-796 ◽  
Author(s):  
Marek Kieliszek ◽  
Kamil Piwowarek ◽  
Anna M. Kot ◽  
Katarzyna Pobiega
Keyword(s):  
Food Industry ◽  
Industrial Applications ◽  
Waste Products ◽  
Yeast Biomass ◽  
Wide Range ◽  
Innovative Solutions ◽  
Biomass Management ◽  
Effective Use ◽  
Modern Technologies ◽  
Biomass Of Microorganisms

AbstractCellular biomass of microorganisms can be effectively used in the treatment of waste from various branches of the agro-food industry. Urbanization processes and economic development, which have been intensifying in recent decades, lead to the degradation of the natural environment. In the first half of the 20th century, problems related to waste management were not as serious and challenging as they are today. The present situation forces the use of modern technologies and the creation of innovative solutions for environmental protection. Waste of industrial origin are difficult to recycle and require a high financial outlay, while the organic waste of animal and plant origins, such as potato wastewater, whey, lignin, and cellulose, is dominant. In this article, we describe the possibilities of using microorganisms for the utilization of various waste products. A solution to reduce the costs of waste disposal is the use of yeast biomass. Management of waste products using yeast biomass has made it possible to generate new metabolites, such as β-glucans, vitamins, carotenoids, and enzymes, which have a wide range of industrial applications. Exploration and discovery of new areas of applications of yeast, fungal, and bacteria cells can lead to an increase in their effective use in many fields of biotechnology.

scholarly journals Potential application of gold nanoparticles in food packaging: a mini review

Gold Bulletin ◽  
2021 ◽  
Author(s):  
Saeed Paidari ◽  
Salam Adnan Ibrahim
Keyword(s):  
Gold Nanoparticles ◽  
Potential Application ◽  
Food Industry ◽  
Food Packaging ◽  
Industrial Applications ◽  
Mechanisms Of Action ◽  
Microbial Load ◽  
Food Sector ◽  
The Past ◽  
Industry Applications

AbstractIn the past few decades, there have been remarkable advances in our knowledge of gold nanoparticles (AuNPs) and synthesizing methods. AuNPs have become increasingly important in biomedical and industrial applications. As a newly implemented method, AuNPs are being used in nanopackaging industries for their therapeutic and antibacterial characteristics as well as their inert and nontoxic nature. As with other NPs, AuNPs have privileges and disadvantages when utilized in the food sector, yet a significant body of research has shown that, due to the specific nontoxic characteristics, AuNPs could be used to address other NP flaws. In this mini review, we present synthesizing methods, food industry applications, and mechanisms of action of gold nanoparticles. Regarding the investigations, gold nanoparticles can play a major role to reduce microbial load in foodstuff and therefore can be implemented in food packaging as an effective approach.

scholarly journals Culture-Independent and Culture-Dependent Characterization of the Black Soldier Fly Gut Microbiome Reveals a Large Proportion of Culturable Bacteria with Potential for Industrial Applications

2021 ◽  
Vol 9 (8) ◽  
pp. 1642
Author(s):  
Dorothee Tegtmeier ◽  
Sabine Hurka ◽  
Sanja Mihajlovic ◽  
Maren Bodenschatz ◽  
Stephanie Schlimbach ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Amplicon Sequencing ◽  
Industrial Applications ◽  
Culture Collection ◽  
Rrna Gene ◽  
Organic Substrates ◽  
Culturable Bacteria ◽  
Black Soldier Fly ◽  
Culture Independent ◽  
Culture Dependent

Black soldier fly larvae (BSFL) are fast-growing, resilient insects that can break down a variety of organic substrates and convert them into valuable proteins and lipids for applications in the feed industry. Decomposition is mediated by an abundant and versatile gut microbiome, which has been studied for more than a decade. However, little is known about the phylogeny, properties and functions of bacterial isolates from the BSFL gut. We therefore characterized the BSFL gut microbiome in detail, evaluating bacterial diversity by culture-dependent methods and amplicon sequencing of the 16S rRNA gene. Redundant strains were identified by genomic fingerprinting and 105 non-redundant isolates were then tested for their ability to inhibit pathogens. We cultivated representatives of 26 genera, covering 47% of the families and 33% of the genera detected by amplicon sequencing. Among these isolates, we found several representatives of the most abundant genera: Morganella, Enterococcus, Proteus and Providencia. We also isolated diverse members of the less-abundant phylum Actinobacteria, and a novel genus of the order Clostridiales. We found that 15 of the isolates inhibited at least one of the tested pathogens, suggesting a role in helping to prevent colonization by pathogens in the gut. The resulting culture collection of unique BSFL gut bacteria provides a promising resource for multiple industrial applications.

scholarly journals Bioaccessibility of Marine Carotenoids

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 397 ◽  
Author(s):  
Isabel Viera ◽  
Antonio Pérez-Gálvez ◽  
María Roca
Keyword(s):  
Pharmaceutical Industry ◽  
Human Health ◽  
Industrial Production ◽  
Food Industry ◽  
Industrial Applications ◽  
Point Of View ◽  
Market Area ◽  
Biological Functions ◽  
Determination Methods ◽  
Nutraceutical Properties

The benefit of carotenoids to human health is undeniable and consequently, their use for this purpose is growing rapidly. Additionally, the nutraceutical properties of carotenoids have attracted attention of the food industry, especially in a new market area, the ‘cosmeceuticals.’ Marine organisms (microalgae, seaweeds, animals, etc.) are a rich source of carotenoids, with optimal properties for industrial production and biotechnological manipulation. Consequently, several papers have reviewed the analysis, characterization, extraction and determination methods, biological functions and industrial applications. But, now, the bioaccessibility and bioactivity of marine carotenoids has not been focused of any review, although important achievements have been published. The specific and diverse characteristic of the marine matrix determines the bioavailability of carotenoids, some of them unique in the nature. Considering the importance of the bioavailability not just from the health and nutritional point of view but also to the food and pharmaceutical industry, we consider that the present review responds to an actual demand.

scholarly journals Screening For Yeast Phytase Leads to the Identification of a New Cell-Bound and Secreted Activity in Cyberlindnera jadinii CJ2

2021 ◽  
Vol 9 ◽  
Author(s):  
Claudia Capusoni ◽  
Immacolata Serra ◽  
Silvia Donzella ◽  
Concetta Compagno
Keyword(s):  
High Temperature ◽  
Phytic Acid ◽  
Convenient Method ◽  
Kluyveromyces Marxianus ◽  
Food Industry ◽  
Yeast Species ◽  
Industrial Applications ◽  
Industrial Processes ◽  
Acidic Ph ◽  
Initial Screening

Phytic acid is an anti-nutritional compound able to chelate proteins and ions. For this reason, the food industry is looking for a convenient method which allows its degradation. Phytases are a class of enzymes that catalyze the degradation of phytic acid and are used as additives in feed-related industrial processes. Due to their industrial importance, our goal was to identify new activities that exhibit best performances in terms of tolerance to high temperature and acidic pH. As a result of an initial screening on 21 yeast species, we focused our attention on phytases found in Cyberlindnera jadinii, Kluyveromyces marxianus, and Torulaspora delbrueckeii. In particular, C. jadinii showed the highest secreted and cell-bound activity, with optimum of temperature and pH at 50°C and 4.5, respectively. These characteristics suggest that this enzyme could be successfully used for feed as well as for food-related industrial applications.

Plasma-treated water affects Listeria monocytogenes vitality and biofilm formation

2020 ◽  
Author(s):  
Oliver Handorf ◽  
Viktoria Isabella Pauker ◽  
Thomas Weihe ◽  
Uta Schnabel ◽  
Eric Freund ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Food Industry ◽  
Microwave Plasma ◽  
Industrial Applications ◽  
Bacterial Biofilm ◽  
Treated Water ◽  
Force Microscopy ◽  
Cell Vitality ◽  
Pretreatment Time

Abstract Plasma-generated compounds (PGCs) such as plasma-processed air (PPA) or plasma-treated water (PTW) offer an increasingly important alternative for the treatment of microorganisms in hard-to-reach areas found in several industrial applications including the food industry. To this end, we studied the antimicrobial capacity of plasma-treated water on the vitality and biofilm formation of Listeria monocytogenes, a common food spoilage microorganism. Using a microwave plasma (MidiPLexc), 10 ml of deionized water was treated for 100 s, 300 s and 900 s (pretreatment time) and the bacterial biofilm was subsequently exposed to the PTW for 1 min, 3 min and 5 min (posttreatment time) for each pretreatment time separately. Colony-forming units (CFU), metabolic activity, and cell vitality were reduced for 4.7 log10, 47.9%, and 69.5%, respectively. Live/dead staining and fluorescence microscopy showed a positive correlation between treatment and incubation times and reduction in vitality. Atomic force microscopy indicated a change in the plasticity of the bacteria. These results suggest a promising antimicrobial impact of plasma-treated water on Listeria monocytogenes, which may lead to more targeted applications of plasma decontamination in the food industry in the future.

scholarly journals Invasive Seaweeds in the Iberian Peninsula: A Contribution for Food Supply

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 560
Author(s):  
Diana Pacheco ◽  
Glacio Souza Araújo ◽  
João Cotas ◽  
Rui Gaspar ◽  
João M. Neto ◽  
...  
Keyword(s):  
Iberian Peninsula ◽  
Food Industry ◽  
Algal Species ◽  
Industrial Applications ◽  
Indigenous Species ◽  
Invasion Mechanisms ◽  
Daily Food ◽  
Research Findings ◽  
Non Indigenous Species ◽  
Nutraceutical Properties

The introduction of exotic organisms in marine ecosystems can lead to economic and ecological losses. Globally, seaweeds represent a significant part of these non-indigenous species (NIS), with 407 introduced algal species. Furthermore, the presence of NIS seaweeds has been reported as a major concern worldwide since the patterns of their potential invasion mechanisms and vectors are not yet fully understood. Currently, in the Iberian Peninsula, around 50 NIS seaweeds have been recorded. Some of these are also considered invasive due to their overgrowth characteristic and competition with other species. However, invasive seaweeds are suitable for industrial applications due to their high feedstock. Hence, seaweeds’ historical use in daily food diet, allied to research findings, showed that macroalgae are a source of nutrients and bioactive compounds with nutraceutical properties. The main goal of this review is to evaluate the records of NIS seaweeds in the Iberian Peninsula and critically analyze the potential of invasive seaweeds application in the food industry.

scholarly journals Significance of Inoculum Size in the Lag Time ofListeria monocytogenes

2000 ◽  
Vol 66 (4) ◽  
pp. 1706-1710 ◽  
Author(s):  
Jean-Christophe Augustin ◽  
Agnès Brouillaud-Delattre ◽  
Laurent Rosso ◽  
Vincent Carlier
Keyword(s):  
Listeria Monocytogenes ◽  
Food Industry ◽  
Lag Time ◽  
Inoculum Size ◽  
Nutrient Concentrations ◽  
Predictive Microbiology ◽  
Oflisteria Monocytogenes

ABSTRACT The lag time of Listeria monocytogenes growing under suboptimal conditions (low nutrient concentrations, pH 6, and 6.5°C) was extended when the inoculum was severely stressed by starvation and the inoculum size was very small. Predictive microbiology should deal with bacterial stress and stochastic approaches to improve its value for the agro-food industry.

scholarly journals Pressure Loss in Ducts by Dissipative Splitter Silencers: Comparative Study of Standardized, Numerical and Experimental Results

2021 ◽  
Vol 11 (22) ◽  
pp. 10998
Author(s):  
Bartosz Chmielewski ◽  
Iván Herrero-Durá ◽  
Paweł Nieradka
Keyword(s):  
Finite Element Method ◽  
Comparative Study ◽  
Pressure Loss ◽  
Industrial Applications ◽  
Flow Speed ◽  
Point Of View ◽  
Experimental Measurements ◽  
Sound Waves ◽  
The Finite Element Method ◽  
Input Face

Dissipative splitter silencers are widely used in industry for the reduction of propagated sound waves in ducts. Even though these systems are effective from the acoustics point of view when they are properly designed, they also introduce a pressure loss in the system, due to the modification of the properties of the flow circulating inside the duct. This effect is not desired in some industrial applications, so it is necessary to be able to predict the pressure loss as precisely as possible to design silencers according to the needs. Nevertheless, the prediction made by standards are usually limited to given geometries or flow speed. In this work, we present a comparative study on the results obtained for the pressure loss by means of the standards ISO 14163 and VDI 1801-1, numerical simulations with the finite element method, and experimental measurements. Additionally, two different profile shapes and four input face velocities are tested in order to know the influence of these parameters in the variations of the flow and the accuracy of the prediction of the different methods.

scholarly journals Ameliorating process parameters for zeaxanthin yield in Arthrobacter gandavensis MTCC 25325

2020 ◽  
Author(s):  
Shristi Ram ◽  
Sushma Rani Tirkey ◽  
Madhava Anil Kumar ◽  
Sandhya Mishra
Keyword(s):  
Process Parameters ◽  
Bacterial Isolate ◽  
Regression Coefficient ◽  
Culture Collection ◽  
Inoculum Size ◽  
Harvest Time ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Ccd Matrix ◽  
Central Composite

Abstract The present study aims to escalate the production of prophylactic agent zeaxanthin using a screened potential bacterial isolate. For this purpose, a freshwater bacterium capable of producing zeaxanthin was isolated from Bor Talav, Bhavnagar. The 16S rRNA sequence confirmed the isolate as Arthrobacter gandavensis. The bacterium was also submitted to Microbial Type Culture Collection, CSIR-Institute of Microbial Technology, Chandigarh, India, with the accession number MTCC 25325. The chemo-metric tools were employed to optimise the influencing factors such as pH, temperature, inoculum size, agitation speed, carbon source and harvest time on zeaxanthin yield. Thereafter, 6 parameters were narrowed down to 3 factors and were optimised using the central composite design (CCD) matrix. Maximum zeaxanthin (1.51 mg/g) was derived when A. gandavensis MTCC 25325 was grown under pH 6.0, 1.5% (w/v) glucose and 10% (v/v) inoculum size. A high regression coefficient (R2=0.92) of the developed model indicated the accurateness of the tested parameters. To the best of our knowledge, this is the first report on tailoring the process parameters using chemo-metric optimisation for escalating the zeaxanthin production by A. gandavensis MTCC 25325.

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